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RESPON PENGGUNAAN BERBAGAI BAHAN AKTIVATOR PADA AKTIVASI PARTENOGENESIS OOSIT KAMBING HASIL IVM Kholifah Holil, Eva Ari Wahyuni, Hari Soepriandono Gatot Ciptadi
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Parthenogenetic activation is one method that can be used to determine the quality of IVM oocytes results before further use to other reproductive technologies (IVF and transfer core). In parthenogenetic activation can be used various activators such as ethanol, Ca Ionophore, and Crude Sperm Extract (CSE). Therefore, the aim of this experiment is to know the response use a variety of materials activator of parthenogenetic activation of goat oocytes IVM.The sample used in this experiment was oocytes aspirated from goat ovarian follicles taken from RPH Sukun of Malang. Oocytes were matured for 24 hr in TCM-199 supplemented with fetal bovine serum (FBS), follicle-stimulating hormone (FSH) and lutheinizing hormone (LH) at a temperature of 38,5oC and 5% CO2 in humidified air. After another 30 hours of in vitro maturation, they were then activated by various treatments. The treatment of experiment are treatment 1, activation using ethanol 7% for 7 minute, treament 2, activation using Ca Ionophore 20 µM for 7 minute. Treatment 3, activation using CSE 2,5 µg/ml for 2 hr.Based on the result of research, it is showed that activation by using 7% ethanol for 7 minutes is able to produce cleavage rate of 70.40%. Activation by Ca Ionophore 20 μM for 7 minutes is able to produce cleavage rate of 52.75%. While the use of CSE activation with 2.5 ug / ml for 2 hours produces cleavage rate of 36.33%. Thus it can be concluded that the goat oocyte IVM able to respond to a variety of materials activators on parthenogenetic activation performed. The highest response given by the successive results goat IVM oocytes activated using 7% ethanol for 7 minutes, 20 μM Ca Ionophore for 7 minutes, and the CSE 2.5 microg / ml for 2 hours.Keywords: Parthenogenetic activation, goat oocytes IVM, etanol, calsium ionophore, Crude Sperm Extract (CSE).

ANTIBIOTIKA, RESISTENSI, DAN RASIONALITAS TERAPI Eka Rahayu Utami
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Bacterial resistance toward antibiotics has become international and serious problem. Rasional therapy campaign has common recently, include correct medication, precise dose, fix therapy periode and efficient cost. Microbes being resistance through some different ways for live survival. Many things can cause this resistance. In the end, there are a lot of harmful consequences in health, economic, also public health aspect. Rational therapy, government regulation, and civil education become some crusial point in bacterial resistance conquer strategy.Keywords: antibiotics, resistance, rational therapy.

KANKER LEHER RAHIM (CANCER CERVIX) SEBAGAI PEMBUNUH WANITA TERBANYAK DI NEGARA BERKEMBANG Cahyawati Arisusilo
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Worldwide, cervical cancer is twelfth most common (Edward, 2010) and the fifth most deadly cancer in women (Globocan, 2002). It affects about 16 per 100,000 women per year and kills about 9 per 100,000 per year (WHO, 2006). Approximately 80% of cervical cancers occur in developing countries (Kent, 2010). Worldwide, in 2008, it was estimated that there were 473,000 cases of cervical cancer, and 253,500 deaths per year (NCC, 2008). Cervical cancer is the leading cause of cancer death in most developing countries (Edianto, 2006). Counted as many as 510,000 new cases occur each years and lasts more than 288,000 deaths from the disease worldwide. Low incidence of this disease in women aged under 25 years old, but the incidence increases in women aged 35 to 40 years and reached a maximum at the age of the 50's. Meanwhile, the incidence of cervical cancer it self continues to increase from about 25 per 100,000 in 1988 to around 32 per 100,000 in 1992 (Edianto, 2006).In 2005, the number of women aged 15-64 years in Indonesia is 65 million and the prevalence of cervical cancer is 50 per 100,000 women. This means the number of patients with cervical cancer is approximately 32 500 patients. Patients with stage I as much as 7% or 2275, stage Ib-IIa by 28% or 9100, and a stage IIB-IV by 65% or 21 125 patients (Rasjidi, 2007). This data showed that cancer cervix detected mostly on late stages, so it commonly called silent diseases.The uterine cervix is the lowest portion of a woman's uterus (womb). Most of the uterus lies in the pelvis, but part of the cervix is located in the vagina, where it connects the uterus with the vagina.

MANAJEMEN PENGELOLAAN LAHAN KRITIS PADA DAS BRANTAS HULU BERBASIS MASYARAKAT (Pilot Project Desa Bulukerto, Kota Batu) Sri Harini, Suyono, Elok Mutiara
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Management of critical farm base on society represents a new approach to all researcher of environment this time. In management of farm base on this society, society invited directly start from planning, formulation of policy, its benefit collection and execution. With this direct participation enable society can calculate directly impact economical and environment (natural resources conservation), because this two aspect represent two inseparable aspect in doing study management of SDA, because having very important role in supporting efficacy of program of Participatory Action Research (PAR). Pursuant to result of discussion and dig of information of society hence a success main problem identified with society member pursuant to antecedent study (research preliminary) which have been conducted by researcher related to program management of critical farm base on society taken as focus in enable ness is critical condition survey of forest farm, socialization of UU No. 32 year 2009 about management and protection of environment, socialization and training of system of terasiring pattern and plant real correct at farm with high inclination ( 450) having economic value and with vision of environment. Marginally cycle of PAR conducted at this enable ness program is to use technical method, mapping, transect, diagram of Venn, change schema and of livelihood analysis. By using this approach is expected by society have awareness in managing and exploiting environment real correctly. Besides society can direct control to all impact and policy of the policy because related to source of their residence environment and living. Keywords : Erosion, Critical Farm, Research Preliminary, Participatory Action Research, Mapping, Transect, Diagram of Venn, Change Schema, Analysis livelihood

OPTIMASI PARAMETER ANALITIK BIOSENSOR UREA BERBASIS IMMOBILISASI UREASE DALAM MEMBRAN POLIANILIN Begum Fauziyah
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Biosensor Urea is made from the way immobilization enzyme urase inside of membrane polianilin with adsorption technique. Analytical parameter optimation from biosensor has done. The biggest difference between analit solution and blanko solution get in pH 7, long wave 580 nm and urease/PAn membrane having big difference intensity in optimum pH (pH 7) is urease/PAn membrane with urease variance 1 mg/mL. The result of characterization urease/PAn membrane as biosensor are concentration connection to absorban urease/PAn having correlation coefficient 0.9863; sensitivity 0.0445; detection limitation 0.6 ppm; variation coefficient (Kv) less than 5% with response around 20 minute. Urease/PAn membrane has stability till 7 days with 6 days of daily measuring repetitions. Key Words : polianilin, urease, urea, biosensor

EFEKTIFITAS KOMPRES DINGIN DAN HANGAT PADA PENATALEKSANAAN DEMAM Nurlaili Susanti
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Fever is a symptom that accompanies some infectious and non infectious diseases. Fever cause metabolic consequences such as dehydration, increasing oxygen consumption and metabolic rate. Treatment of fever can reduce patient discomfort and another symptoms such as fatigue, myalgia, diaphoresis and chills.In addition to antipyretic, the use of physical methods to reduce fever has been widely applied. Physical methods of cooling are the treatment of choice for hyperthermia, but their value in the treatment of fever remains uncertain. Fever treated with tepid-water sponging and combined with antipyretic drugs are more effectively than those treated with antipyretic drugs alone. Tepid-water sponging represents a simple, nonsedating method to combat the metabolic impact of shivering and to induce cutaneous vasodilatation that increases heat loss.

PENGARUH RANGGAS PAKSA DAN SUPLEMENTASI TEPUNG BEKICOT TERHADAP PERTUMBUHAN FOLIKEL YOLK AYAM (Gallus turcicus) Kiptiyah, Hartanto, Lisin
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Research is conducted to examine the effect of fast method’s forced molting and snail flour supplementation on yolk follicle growth of chicken (Gallus turcicus). In this research, female chicken is used with 1.5 years of age and 1.2 ± 2 kg body weight. The treatments include the fast method’s forced f molting (without food for 0 hour, 72 hours, and 168 hours, but with drink ad libitum) and snail flour supplements of 6 %, 12 %, and 18 %. The observation is concerned with yolk follicle growth attributes such as ovary weight and yolk follicle sizes, such as large, medium and small. The data obtained is analyzed by ANOVA. Result indicates that the treatment of fast method’s forced molting and snail flour supplementation does not have effect on yolk follicle growth of chicken (Gallus turcicus). It is evident as reviewed from the ovary weight and from yolk follicle sizes of large, medium and small. Such phenomena is revealed through data related to fast method’s forced molting and snail flour supplementation, in which both are without effect on ovary weight and large follicle size, but with effect on medium and small yolk follicle sizes.Keywords: Chicken, forced molting, snail flour, yolk follicle

KADAR BIKARBONAT SALIVA PENDERITA KARIES DAN BEBAS KARIES Arief Suryadinata
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Saliva plays a role as a buffer so that the ups and downs of the degree of acidity (pH) can be retained. salivary buffer capacity is determined by the bicarbonate concentration of 85%, 14% is determined by the concentration of phosphate and 1% by salivary proteins. Bicarbonate is the main component of saliva to neutralize the acid thus inhibiting the caries process. Based on the role of bicarbonate in maintaining the pH of saliva to remain normal, there may be differences in levels of salivary bicarbonate in subjects with caries and caries-free. This is because subjects with dental caries have the potential for acid formation and a decrease in pH higher than subjects with caries-free. This study aims to determine differences in levels of salivary bicarbonate in subjects with caries and caries-free, studies are observational analytic study. Based on the result showed average levels of bicarbonate in the saliva of caries-free sample is 188.9440 ± 7.11846 ppm while in samples with high caries intensity is 150.9905 ± 9.76628 ppm, then the results of Kolmogorov-Smirnov normality test p-value of 0.200 obtained (p 0.05) in the sample with high intensity of caries and caries-free sample group. The results of the statistical test T-test two sample unpaired in getting the value of p = 0.000 (p 0.05), This means there are significant differences between the levels of salivary bicarbonate in subject with caries and caries-free.

BIOMONITORING: SEBAGAI ALAT ASESMEN KUALITAS PERAIRAN AKIBAT LOGAM BERAT KADMIUM PADA INVERTEBRATA PERAIRAN Dominggus Rumahlatu
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

People’s activity in water area has made this area as waste place from a variety of human’s activity. One contaminant thrown to the seaboard is heavy metal, specifically for cadmium (Cd). Naturally, the pollution sources of heavy metal Cd in water area are coming from geological process and some of human’s activity (antropogenic). The effect of Cd’s contamination to invertebrate water are died effect and ecology’s unbalancing of sea organism varieties. It is because Cd cannot be broken up (non degradable) by living organism and it can be accumulated to the environment. One effort to tackling heavy metal Cd contamination in water is doing biomonitoring accumulation as assessment instrument of water quality.Key words: biomonitoring, biomoitoring accumulation, heavy metal Cadmium, invertebrate water

SINTESIS SENYAWA RISINOLEIL DIETANOLAMIDA MELALUI REAKSI AMIDASI ASAM RISINOLEAT DENGAN DIETANOLAMINA Suci Amalia
SAINSTIS SAINSTIS (Vol 1, No 1
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

There was a synthesis of derived amida of risinoleat acid compound through amidasi reaction. Risinoleat Acid obtained through metil risinoleat isolation from jarak oil through trans-esterifikasi reaction using natrium metoksida, and then doing hidrolisis metil risinoleat. Amidasi reaction to risinoleat acid was obtained by adding dietanolamina to the high temperature during 6 hours. Structure confirmation from the result compound is characterized by spektrometer IR and GC-MS.Amidasi risinoleat acid reaction and dietanolamina produce risinoleil dieanolamida compound as like thick liquid, chocolate yellowness coloured with rendemen 45,26 %. The lost of acid karbonil cluster absorption from analysis IR result to alkanolamida compound cannot convince that amida compound is 100% formed. Key words: risinoleat acid, dietanolamina, amidasi reaction, jarak oil

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Roihatul Muti’ah, Apt, M.Kes

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Kota malang,

Jawa timur

INDONESIA

Abstract

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Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Roihatul Muti’ah, Apt, M.Kes

Contact Email mroihatul@yahoo.com

Phone -

Journal Mail Official -

Editorial Address -

Location Kota malang, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Roihatul Muti’ah, Apt, M.Kes

Contact Email
mroihatul@yahoo.com

Phone
-

Journal Mail Official
-

Editorial Address
-

Location
Kota malang,

Jawa timur

INDONESIA