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INDONESIA
JURNAL PEMULIAAN TANAMAN HUTAN
Published by Badan Penelitian dan Penegembangan Kehutanan
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 249 Documents
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KERAGAMAN GENETIK TIMOHO (Kleinhovia hospita Linn.) BERDASARKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD) ILG Nurtjahjaningsih; Lukman Hakim; Yemima Viktory Utomo; Anto Rimbawanto; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.85-93

Abstract

Timoho (Kleinhovia hospita Linn.) has high economic value for medicine and construction. However, information on genetic diversity and conservation genetic have been limited by the lack of genetic data. Acacia mangium (mangium)This study aimed to assess genetic diversity of timoho using RAPD markers. Leaf samples of seedlings were collected from a nursery at CFBTI in Yogyakarta; the seedlings originated from plantation in Gunung Kidul (GK) and arboretum in Faculty of Forestry, GMU (FK). Seven out of 22 screened RAPD markers were stable in amplification and consisted of 61 polymorphic loci; then these markers were used to analyse genetic diversity and population structure of 48 seedlings from GK and FK. The genetic diversity and structure parameters were calculated by GenAlex software. A moderate level of genetic diversity (mean uHE=0,326) and great genetic distance between the two populations (DA=0.119) were found in this species. PCoA analysis showed that the 48 individuals were divided into two clusters according to their originated population. Appropriate conservation strategies were proposed to protect genetic diversity of this species.
RESPON BIBIT GERONGGANG (Cratoxylum arborescens) TERHADAP PENAMBAHAN KOMPOS DARI LIMBAH PULP vs LIMBAH PULP DAN KERTAS DI TANAH GAMBUT Siti Wahyuningsih; Syofia Rahmayanti
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.105-113

Abstract

Geronggang (Cratoxylum arborescens) is able to adapt to ex-burned peatland. However, the viability of germination seed in peat soil is low. Composted sludge of paper and/or pulp wastewater treatment can provide nutrients to plants. This study compares the vegetative growth of geronggang seedlings after the addition of a composted sludge of pulp, and pulp and paper in peat soil after six months of planting. The sludge was air-dried to gain a water content of 60-70%. Decomposers namely Penicillium citrinum and P. oxalicum with a density of 107(spores.ml-1) were added to the sludge. The composting was held for a month. The sludge compost was added to the seedlings with a dosage equal to 2, 4, 6, 8, 10, 12, 14 and 16 (ton.ha-1). Height and diameter increment of the seedlings were recorded for six months and the data was analyzed using ANOVA. Application of pulp sludge compost at a dosage equal to 16 (ton.ha-1) resulted in a better height increment than other treatments after six months of planting. The addition of pulp sludge compost at a dosage equal to 12 (ton.ha) orpulp and paper sludge compost at a dosage equal to 8 (ton.ha-1) resulted in seedlings with a better diameterincrement. However, those dosages which result in a better height and diameter increment of the seedlings were not statistically different from some treatments. In conclusion, pulp sludge compost is better than pulp and paper sludge compost to enhance the growth of geronggang seedlings in peat soil.
KERAGAMAN GENETIK Taxus Sumatrana DARI POPULASI ALAM DI JAMBI MENGGUNAKAN PENANDA RAPD Istiana Prihatini; Dodi Frianto; Eka Novriyanti; ILG Nurtjahjaningsih; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.95-104

Abstract

The natural population of Taxus sumatrana in several regions in southern Asia including Indonesia has experienced a decline in number. In Sumatra, the population with very few trees was found in Gunung Kerinci and Gunung Tujuh, Kerinci Regency, Jambi. Conservation efforts to protect the extinction of this species are being carried out, one of which is by exploring seeds from natural populations to be planted ex situ. Information on the genetic diversity of Taxus is needed to determine a conservation strategy for this species. This researchwas conducted to obtain information on genetic diversity of Taxus sumatrana. Ten RAPD markers were used to analyze the genetic diversity of 44 individuals from two natural populations, namely Gunung Tujuh and Gunung Kerinci. These markers resulted in 104 loci which were used in the RAPD data analysis using GenAlex ver.6.5 program. The results of the data analysis showed that heterozigosity which showed the level of genetic diversity of the populations of the two populations observed was in moderate level and similar, namely 0.33±0.14 (Gunung Tujuh) and 0.35±0.14 (Gunung Kerinci). The genetic distance between the two populations was also in a moderate value (0.097) and the similarities in genetic structure based on the RAPD markers were showinggenetic variety in moderate level (0.908). The results indicate that the two populations, although geographically close have moderate level of genetic structure. Genetic conservation efforts for future Taxus species could increase the number of individuals from both locations and separate them as two distinct populations.
PEMBENTUKAN EMBRIO SOMATIK DARI EKSPLAN DAUN RAMIN, SPESIES TANAMAN LANGKA Yelnititis Yelnititis
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.129-135

Abstract

Ramin (Gonystylus bancanus (Miq.) Kurz) is one of the most valuable timbers in Indonesia and the most over-exploited woody species, on the other hand, the success in propagation techniques are still limited. This species has been listed in Appendix II of CITES since 2004 as the number of trees and populations continuously decline. Tissue culture has been explored for mass propagation, however, this technique still faces the challenge, mainly in shoot elongation regenerated from single node explants.  The purpose  of  these experiments is  to select the best auxin (2,4-D, picloram, dan dicamba) and the best concentration of Benzyl Adenine (BA)  treatment on  somatic embryo  formation. A series of experiments of somatic embryogenesis from leaf explant for ramin were conducted at Tissue Culture Laboratory, Center for Forest Biotechnology and Tree Improvement Yogyakarta. Modified Murashige and Skoog (MS) media supplemented with 0,1 mg/L thiamine; 0,5 mg/l nicotinic acid; 0,5 mg/L pyridoxine; 2.0 mg/L glycine and 100 mg/L myo-inositol were used as growth medium. In this study, three different auxins were used as treatments: 2,4-D (2,4 dichlorophenoxy acetic acid), picloram, and dicamba applied at 6.0 mg/L. The observation was made on the texture of callus formed and the performance of the somatic embryos obtained. The results showed that the texture of callus obtained is compact formed callus and green in color. The best treatment to induce globular somatic embryos is using 6.0 mg/L picloram within eight months. The best treatment to induce globular and torpedo somatic embryos is BA 3.0 mg/L treatment.
PENGARUH BERBAGAI KONSENTRASI GLUTAMIN TERHADAP PERTUMBUHAN TUNAS Aquilaria malaccensis Lamk. SECARA KULTUR JARINGAN Chrisnanda Ayu Melati; Etty Handayani; Toni Herawan
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.145-151

Abstract

Aquilaria malaccensis Lamk. is a type of plant that is threatened with extinction due to the high level of illegal collection from the forest. Tissue culture techniques provide an alternative to vegetative propagation on a large scale for conservation efforts. The addition of glutamine to the culture media was intended to increase the growth of A. malaccensis Lamk shoots. The research was conducted with quantitative research methods using single factor laboratory experiments with four (4) treatments arranged in a completely randomized design (CRD). The treatments were differentiated based on the addition of glutamine, namely, G0: 0 mg / l; G1: 10 mg / l; G2: 20 mg / l; G3: 30 mg / l. Data analysis used Analysis of Variance (Annova) with a level of α = 5%. The results showed that Glutamine could't affect the growth of A. malaccensis Lamk, but there was the best trend in the addition of Glutamine 20 mg/l.
INDUKSI KALUS DARI EKSPLAN BIJI IMMATURE KEPEL (Stelechocarpus burahol (Bl.) Hook.f. & Th.) SECARA IN VITRO Tri Suwarni Wahyudiningsih; Noor Farid; Esna Dilli Novianto; Tia Noviantika
Jurnal Pemuliaan Tanaman Hutan Vol 16, No 1 (2022): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2022.16.1.1-9

Abstract

Endosperm in immature seeds of S. burahol can be used as explants for callus induction which is triploid. The aim of the study was to induce callus from immature seed explants of S. burahol on Murashige & Skoog media with NAA and picloram concentration treatments. The research was carried out from April to October 2021 at the Tissue Culture Laboratory at KBTPH Salaman and the Faculty of Agriculture, Tidar University. The first study used two-factor RAL, the first factor: the concentration of NAA (N0, N2, N4, N6, N8, N10 mg/L). The second factor: seed diameter (D1 = 0.3 cm, D2 = 0.6 cm, D3 = 0.9 cm). Research II used RAL, the single factor was picloram concentration (P0; P0,5; P1; P2; P4; P8 mg/L). Research I. Explants stretched at 3 days after planting and produced 6 callus from 1 week after planting (weeks after planting). Three callus were formed in treatment N4D1 and one callus each in treatment N0D3, N4D3 and N6D12. Callus textured crumbs and compact. Callus color is white, transparent white, and greenish yellow. One liver and cotyledon somatic embryos were found in the N2D1 treatment. In the second study produced 17 callus. At 0.5 Picloram; 1; 2 mg/L produced compact and crumb callus, callus color was white and transparent. At 4 and 8 mg/L picloram produced a white compact callus. The success of callus induction is still small, so it is necessary to continue the use of types and concentrations of auxins and cytokinins and younger explants of immature seeds. The higher number of callus can be analyzed for ploidy level so that triploid plant material can be produced
ANALISIS PERTUMBUHAN TANAMAN UJI SPESIES MERANTI (Shorea spp) dan KERUING (Dipterocarpus spp) DI IUPHHK-HA PT. ERNA DJULIAWATI KALIMANTAN TENGAH Abdurrani Muin; Diana Citra Dewi; Reine Suci Wulandari
Jurnal Pemuliaan Tanaman Hutan Vol 16, No 1 (2022): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2022.16.1.11-22

Abstract

Meranti is the dominant native species in the IUPHHK-HA PT. Erna Djuliawati in Central Kalimantan. Its use is quite a lot and its existence is dominant as well as a native species, so that this species becomes a top priority in forest development activities in logged-over areas. To get the right species according to the ecological conditions of the site to be planted, PT. Erna Djuliawati conducted a species trial in the IUPHHK-HA area of Central Kalimantan. In the species test plot, the company has planted as many as 20 types of meranti from its own area since 2005. The purpose of this study was to analyze differences in growth and determine the best species in the IUPHHK-HA area. Species trials were carried out using a Randomized Complete Treatment Block Design (RCBD). Parameters measured in this study were total height, diameter, percentage of life and increment of diameter. Based on the analysis results, it turns out that there are differences in growth between 20 species of meranti aged 15 years tested in the species test plot. The fast growing and superior species are Shorea macrophylla, Shorea platyclados and Shorea parvofolia. Other species that can be developed in the IUPHHK-HA area are Shorea johorensis, Shorea johorensis and Shorea stenoptera. Keywords:,Dipterocarpus spp,growth, Shorea spp, species test
VARIASI MORFOLOGI DAUN JELUTUNG DARAT (DYERA COSTULATA) DAN JELUTUNG RAWA (DYERA POLYPHYLLA) Arniana Anwar; Iskandar Z Siregar; Ulfah Juniati Siregar; Laswi Irmayanti; Reyna Ashari
Jurnal Pemuliaan Tanaman Hutan Vol 16, No 1 (2022): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2022.16.1.23-33

Abstract

Jelutung is a tree species of the family Apocynaceae that is famous for its latex use as material for chewing gum. In Indonesia there are two species, namely jelutung land (Dyera costulata) and jelutung swamp (Dyera polyphylla). Until now, information on the differences in the two types of jelutung morphology is still very limited. There is therefore the need for a study on the variation of both types cultivated. This study was conducted to estimate variations in leaf morphology for land and swamp jelutung to assess 14 variables of its leaf morphology. Results of multivariate analysis (PCA, MCA, and CDA) showed that jelutung can be classified into two groups: D. costulata and D. polyphylla. PCA and MCA showed three variables that most distinguishes the two types of jelutung consecutive leaf length (PL), Roving Leaf (KL) and the shape of apex (AS). The CDA variables that play a major role in the classification of Dyera are the shape of apex (AS), the form of the base of the leaf (BS), leaf vein angle (SD).
KELIMPAHAN POPULASI DAN SERANGAN ULAT Heortia vitessoides PADA TANAMAN PENGHASIL GAHARU (Aquilaria microcarpa) DI KALIMANTAN SELATAN Fajar Lestari; Beny Rahmanto
Jurnal Pemuliaan Tanaman Hutan Vol 16, No 1 (2022): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2022.16.1.35-43

Abstract

Strategy of prevention and control of agarwood pest need information about intensity of attacks and abundance of Heortia vitessoides larvae population. This study aims to get a percentage of attacks intensity and abundance of pest of H. vitessoides larvae populations in South Kalimantan. Observations and data collection was done every month by damage leaf scoring and counting the number of larvae population. Observation plots consist of 2 plots for observation of attacks intensity at each location, 5 plots for observation of larvae population abundance in Gumbil and 4 plots in Layuh. Total observations plot made were 13 plots. The results showed average of the highest intensity of larvae attacks (22.9%) occurred in September and the number of the largest larvae population (121.53 individuals/tree) in February in Gumbil village, Hulu Sungai Selatan. The  average of highest intensity of larvae attacks (21%) occurred in February ke dua pengamatan and the highest population of larvae (37.71 individuals/tree) in February ke tiga pengamatan in Layuh village, Hulu Sungai Selatan, South Kalimantan. Keywords: agarwood, Heortia vitessoides,intensity, populationABSTRAKInformasi kelimpahan populasi dan intensitas serangan suatu hama diperlukan dalam rangka pengambilan keputusan strategi pencegahan dan pengendalian yang akan dilakukan. Kelimpahan populasi ulat dan intensitas serangan hama ulat tanaman penghasil gaharu di Kalimantan Selatan bersifat fluktuatif dari waktu ke waktu. Penelitian ini bertujuan mendapatkan informasi kelimpahan populasi dan persentase intensitas serangan hama ulat Heortia vitessoides di desa Gumbil dan Layuh provinsi Kalimantan Selatan. Pengamatan dan pengambilan data dilakukan setiap bulan dengan melakukan penghitungan jumlah populasi ulat serta skoring kerusakan daun. Pengamatan kelimpahan populasi ulat di Gumbil terdiri dari 5 plot, setiap plot terdiri dari 12 – 36 batang sedangkan di Layuh 4 plot dengan jumah tanaman 12 – 24 batang. Plot pengamatan intensitas serangan terdiri dari 2 plot di Gumbil dengan jumlah tanaman 12 batang disetiap plot dan  2 plot  di Layuh dengan jumlah tanaman 14 batang disetiap plot. Total plot pengamatan yang dibuat adalah 13 plot. Hasil penelitian menunjukkan tahun ke tiga pengamatan di Gumbil rataan intensitas serangan ulat paling tinggi (22,9%) terjadi pada bulan September dan jumlah populasi ulat paling banyak (121,53 ekor/pohon) pada bulan Februari. Sementara itu di Layuh  rataan intensitas serangan ulat paling tinggi (21%) terjadi pada bulan Februari tahun ke dua pengamatan dan jumlah populasi ulat paling banyak (37,71 ekor/pohon) pada bulan Februari tahun ke tiga pengamatan. Dinamika kelimpahan populasi dan intensitas serangan ulat H. Vitessoides sangat dipengaruhi oleh ketersediaan pakan dan faktor lingkungan tempat tumbuh tanaman inang. Kata kunci : gaharu, Heortia vitessoides, intensitas, populasi

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