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Journal of Tropical Biodiversity and Biotechnology

jtbb
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Published by Universitas Gadjah Mada

ISSN : 25409573 EISSN : 25409581 DOI : -

Core Subject : Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.

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Articles 5 Documents

Search results for , issue "Vol 1, No 2 (2016): December" : 5 Documents clear

Spesies Tumbuhan Penyusun Vegetasi Lantai di Wilayah Restorasi Taman Nasional Gunung Merapi di Ngablak, Magelang, Jawa Tengah Purnomo Purnomo; Retno Peni Sancayaningsih; Dian Wulansari
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 2 (2016): December
Publisher : Universitas Gadjah Mada

Vegetation restoration by Mount Merapi National Park (TNGM) in Ngablak, Magelang, Central Java need the floristic data composition of understory vegetation. The objectives of this research were to identify and to determine the importance values of each plants species that composed of that restoration area. Sample collections were conducted using survey method for their morphological characters as voucher specimens that were used for species identification. Vegetation analysis was conducted using the quadratic method; shrubs (5x5 m2), grasses and herbs (1x1 m2), and importance values were analysed using relative frequencies and densities. The result shows that understory vegetation on the restoration area in Ngablak was composed of 60 seed plant species from 18 plant families. There were 7 plant families that dominate the region; Papilionaceae (13 species), Asteraceae (11), Poaceae (Grasses) (10), Cyperaceae (Sedges) (5), Amaranthaceae (3), Mimosaceae (3) and Commelinaceae (3). Plants species that have important value more than 25% in this restoration area were Cymbopogon citratus (sereh), Mimosa pudica (Putri malu), Gomphrena serrata (Bunga kancing), Pannisetum purpureum (Rumput gajah), Ageratum conyzoides (Wedusan), dan Euphatorium inulifolium (kirinyuh). The external factors that influence the growth of the understory vegetation are pH of soil and soil moisture.

Peptone and tomato extract induced early stage of embryo development of Dendrobium phalaenopsis Orchid Nintya Setiari; Aziz Purwantoro; Sukarti Moeljopawiro; Endang Semiarti
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 2 (2016): December
Publisher : Universitas Gadjah Mada

Germination and growth of orchid seeds can be accelerated by the addition of organic supplement and plant extract in culture medium. The objective of this study was to determine the effect of peptone and tomato extract on early stage of embryo development of Dendrobium phalaenopsis orchids. Orchid seeds were sown on NP and VW medium with addition of 10% of CW (NPCW and VWCW). Five weeks after seed germination, about 58.03% seed germination was observed on VWCW medium, and only 37.45% seed germination on NPCW. Tomato extract and peptone were added in VWCW, resulting VWCWTP medium. After 4-8 weeks on VWCWTP, 94.42% seeds was germinated into plantlet, but only 67.30% germinated seeds on VWCW. To get optimal growth and development of D. phalaenopsis orchids embryos in the in vitro condition, supplement of 100 ml.L-1 coconut water, 100 mg.L-1 tomato extract and 2 mg.L-1 peptone into VW basic medium is required.

Isolation and Characterization of Lactic Acid Bacteria from Inasua Ferymon Mahulette; Nisa Rachmania Mubarik; Antonius Suwanto; Widanarni Widanarni
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 2 (2016): December
Publisher : Universitas Gadjah Mada

Inasua is a traditionally product of wet salt fish fermentation produced by Teon, Nila and Serua (TNS) Communities in Central Maluku, Indonesia. The community made this fermented fish to anticipate the lean time when fisherman could not go to sea. The fish that used as inasua raw material is demersal fishes that live around coral reefs, such as Samandar fish (Siganatus guttatus), Gala-gala fish (Lutjanus sp.) and Sikuda fish (Lethrinus ornatus). The objective of the research was to isolate and characterize of bacterial indigenous in Inasua from three producers in Seram Island. The measurement of pH from inasua samples were 5.9, 5.0 and 5.8, respectively. The highest number of lactic acid bacteria was found from Gala – gala inasua was 2,5x107 cfu/g sample. Isolation of all isolates bacteria from inasua showed that a total of 7 isolates of bacteria was obtained from Samadar inasua, 9 isolates from Gala-gala inasua, and 7 isolates from Sikuda inasua. From a total of 23 isolates, only 6 isolates had characteristic as lactic acid bacteria that were Gram positive, negative catalase, and cocci shape. The microscopic characteristics of the isolates are coccid in pairs or uniforms which combine to form tetrads. Carbohydrate utilization test of selected isolate by using API 50 CHB kit indicated that 13 carbohydrates are fermented by these isolates after incubation for 48 hours. The research was concluded that the dominant bacteria in inasua sample is cocci-lactic acid bacteria.Keywords : fermented fish, inasua, lactic acid bacteria, MRSA medium

Biomass Production of Root and Shoot of Talinum paniculatum Gaertn. by Liquid and Solid Ms Medium with Plant Growth Hormone IBA Muhammad Hamzah Solim; Y. S. Wulan Manuhara
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 2 (2016): December
Publisher : Universitas Gadjah Mada

Talinum paniculatum Gaertn. is one of traditional medicinal plant in Indonesia which has benefits such as for vitality and maintain blood circulation. The aim of this research is to obtain biomass production of root and shoot of T. paniculatum Gaertn. by liquid and solid MS medium with IBA. This research conducted to provide biomass as raw material for secondary metabolites test. Stems as explant were induced with four treatments (liquid MS, solid MS, liquid MS + 2 ppm IBA and solid MS + 2 ppm IBA) with five repetitions. Observation did for 28 days. The parameters are the percentage of explants which formed the root and shoot, morphology, fresh and dry biomass. Result shows that percentage of root and shoot have 100% in liquid and solid MS + 2 ppm IBA. Fresh and dry biomass of root and shoot in solid MS + 2 ppm IBA higher than the others. This research found callus in liquid and solid MS + 2 ppm IBA. Morphology of root in liquid MS has thin and friable, but thick in solid MS. Shoot in solid and liquid MS has thin, short and sturdy.

Moluska Benthik di Perairan Lima Muara Sungai Kawasan Teluk Lamong, Surabaya, Jawa Timur Ucu Yanu Arbi
Journal of Tropical Biodiversity and Biotechnology Vol 1, No 2 (2016): December
Publisher : Universitas Gadjah Mada

Observation on mollusk on five estuary of Lamong Bay waters was conducted on June and October 2005. The aim of this study was to determine condition and community structure of molluscs living in those areas. Samples were collected using Eckmann grab and sieved through 0.5 mm mesh-sized. The number of mollusk species was 21 species, consist of 8 species class of Gastropoda and 13 species class of Bivalvia. A diversity index (H) ranged 0.802 to 1.028, an evenness index (J) was 0.923 to 1 and a richness index (D) was 15.126 to 20.959. In which Tellina palatum (Tellinidae) and Placemen calophyla (Veneridae) class of Bivalvia relatively wide distributed. In generally, this result showed that five estuary of Lamong Bay waters has a relatively low molluscs diversity.

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Daerah istimewa yogyakarta

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