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INDONESIAN JOURNAL OF PHARMACY

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Published by Universitas Gadjah Mada

ISSN : 23389427 EISSN : 23389486 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.

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Articles 8 Documents

Search results for , issue "Vol 13 No 4, 2002" : 8 Documents clear

In Vitro CYTOTOXIC ASSAY OF CHLOROFORM EXTRACT OF Brucea javanica L .(Meer), Ipomoea batatas Poir., Mussaenda pubescens Ait.F., AND Portulaca oleracea L. TO HeLa CELLS M, Sonlimar; ., Sismindari; ., Mustofa
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp215-222

Some plants, such as Ipomoea batatas Poir., Mussaenda pubescens Ait. F., Portulaca oleracea L., Brucea javanica L. (Meer), have been used traditionally to treat cancer, even though the scientific base of this activity has yet been completely investigated. The study was conducted to evaluate the cytotoxic activity of these plants on HeLa cell lines.The cytotoxicity of chloroform extract from these plants were determined using micro radioactive method by measuring an incorporation (3H)-hipoxanthine on the cells after 24 and 48 hours incubation with each extract, using doxorubicine as a positive control. Extract free cell culture was refered to as 100% growth. IC50 value, showing 50% inhibition of the cell growth, was determined based on the concentration versus percentage inhibition curves.The result showed the extract B. javanica was the most active to hela cells with the IC50 value of 13.69 ï‚±1.07 and 55.7ï‚±8.4 ïg/ml after 24 and 48 hours incubation respectively.Key words: Ipomoea batatas Poir., Mussaenda pubescens Ait. F., Portulaca oleracea L., Brucea javanica L. (Meer), Cytotoxic, HeLa cell lines.

MOLECULAR MODELING OF VITEOSIN-A, A TRACHEOSPASMOLYTIC COMPOUND ISOLATED FROM N-HEXANE EXTRACT OF THE LEAVES OF Vitex Trifolia L. Alam, Gemini; Sudarmanto, B.S. Ari; Astuti, Puji; Wahyuono, Subagus
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Viteosin-A, a tracheospasmolytic compound, was successfully isolated from n-hexane extract of the leaves of Vitex trifolia L. With the concentration of 0.05 and 0.15 mg/ml viteosin-A inhibited a guinea pig tracheal contraction due to histamine (10-7 â€“ 10-3 M) in vitro by 27.1 and 47.9 %, respectively. Confirmation of C-5 and C-6 configuration is necessary to determine the active reaction site of viteosin-A and its receptor for future development. This research was focused on a molecular modeling of viteosin-A using computational method with HyperChem Pro 4.0 for Windows as software. Based on spectroscopic data and molecular modeling, viteosin-A has S configuration at C-5 and C-6, and therefore was confirmed as (5S,10S)-6S-acetoxy-8R-methyl-9ï¡-hidroxy-labda-13Z-en-16,15-olide.Keywords: viteosin-A, molecular modeling

QUANTITATIVE ANALYSIS OF GENERIC AND BRANDED NAME AMPICILLIN IN TABLETS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) Putra, Effendy De Lux
INDONESIAN JOURNAL OF PHARMACY Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

The determination of ampicillin in tablets of branded and generic names by reversed phase high performance liquid chromatography (HPLC) had been carried out. Using column selectosphere C18 (25cm x 4,6 mm) as stationary phase; water : acetonitrile : potassium phosphate 1 M : acetic acid 1 N (909+80+10+1) V/V as mobile phase; flow rate = 2,5 ml/minute; sensitivity = 0,08 AUFS and uv detector at 254 nm wave length. Identification of ampicillin BPFI, ampicillin (Pharos), and ampicillin in tablets of branded and generic names got the retention time 5 minutes.Determination of calibration curve linearity showed a linear correlation between the peak area versus concentrations from 200 to 700 ïg/ml with the correlation coefficient, r = 0,9984 and got the equation of regression Y = 249,89 X - 4305,15. The recovery test of ampicillin (Pharos) showed the concentration 99,36%ï£ïï£104,76% with deviation standard = 1,6447; relative error = 2,06% and coefficient of variation = 1,61%. The quantification of ampicillin in tablets with generic names showed the concentration = 99,69%ï£ïï£104,99% (PT. Indofarma); 91,03%ï£ïï£97,53% (PT. Phapros); 95,70%ï£ïï£102, 44% (PT. Farma); and for tablets of branded names namely : Binotal (PT. Bayer) = 93,38%ï£ïï£99,44%; Kalpicillin (PT. Kalbe Farma) = 91,41%ï£ïï£97, 95%; Parpicilin (PT, Prafa) = 97,19%ï£ïï£101,67% and Cetacilin (PT. Soho) = 95,60%ï£ïï£98,58%. All samples fulfilled the requirement of Farmakope Indonesia fourth edition (1995) namely containing ampicillin not less than 90,0% and not more than 120,0% from the label declared.Key words : HPLC, Ampicillin, assay

IDENTIFICATION OF Klebsiella pneumoniae RESISTANT MUTANTS AGAINST BRL 41897A ANTIBIOTIC USING SOUTHERN BLOT ANALYSIS. M. Kuswandi
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Isolation of Klebsiella pneumoniae resistant mutants against BRL 41897A antibiotics using TnphoA has yielded 30 mutants which have been characterized. In order to identify whether the mutants are as a result of the insertion (transposition) of TnphoA, and to count how many copies of TnphoA are inserted in the each mutant chromosome, several chromosomal DNA of the mutants have been cut with restriction enzymes and analised using Southern blot method and DIG conjugation. The results showed that at least one mutant (KSL19) carried one copy of transposon, whereas at least 2 mutants (KSL38 and KSL52, ) have 2 copies. It is obvious that TnphoA mutagenesis provides an efficient method of selecting mutants which are defective in genes controlling expression of the outer membrane and the periplasmic membrane proteins.Key words: Resistant K. pneumoniae mutants- TnphoA mutagenesis – BRL 41897A- Southern Blot.

SYNTHESIS AND ANTIMICROBIAL ACTIVITY EVALUATION OF ETHYL SALICYL FUMARATE AND ETHYL FURFURYL FUMARATE Jumina .; Iqbal Tahir; Abdul Karim Zulkarnain
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

This research was conducted in order to synthesize and investigate the antimicrobial activity of ethyl salicyl fumarate and ethyl furfuryl fumarate. These two target molecules were chosen as the former is the derivative of C-9154 antibiotic containing phenolic hydroxyl group, whereas the latter is an example of C-9154 antibiotic derivative bearing furan ring instead of benzene.Ethyl salicyl fumarate was synthesized from methyl salicylate through reduction with LiAlH4, condensation of salicyl alcohol with maleic anhydride, and esterification of salicyl maleic acid with ethanol in the presence of benzenesulfonic acid as the catalyst. These reactions gave satisfactory yields (76-92 %) in all steps involved. Similar to this procedure, ethyl furfuryl fumarate was prepared from furfural through reduction with NaBH4 followed by condensation of the resulted furfuryl alcohol with maleic anhydride and esterification of furfuryl maleic acid with ethanol in the presence of benzenesulfonic acid. Although the reduction of furfural and the condensation of furfuryl alcohol with maleic anhydride could be performed smoothly, the esterification of furfuryl maleic acid with ethanol only gave 38 % yield of ethyl furfuryl fumarate.The results of antimicrobial activity test showed that the value of minimum inhibition concentration (MIC) of salicyl maleic acid and ethyl salicyl fumarate towards Staphyllococcus aureus were 500 and 100 μg/mL, whereas towards Eschericia coli were 2000 and 4000 μg/mL respectively. In contrast, the MIC values of furfuryl maleic acid and ethyl furfuryl fumarate towards Staphyllococcus aureus and Eschericia coli were 150 and 100 μg/mL respectively.Keywords: synthesis, activity, C-9154 antibiotic, fumarate

THE CLEAVING ACTIVITY ASSAY ON SUPERCOILED DNA BY PROTEIN FRACTIONS FROM Morinda citrifolia LEAVES Nanik Sulistyani; Sismindari .; Sudjadi .
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Study on supercoiled DNA cleaving activity assay of protein fraction from Morinda citrifolia leaf had been done. The aim of this research is to compare the supercoiled DNA cleaving abilities among the fractions. This is a preliminary screening to find RIP from Morinda citrifolia Fractionation of protein was done by adding Ammonium Sulphate up to 20%, 40%, 60%, 80% and 100% in the crude extract, saturated solution was then centifugated to get the pellet fractions and coded as F-20, F-40, F-60, F-80 and F-100. The mixture of pUC19 plasmid and protein fractions, in the same protein concentration were incubated at room temperature for one hour, the cleaving ability of protein fraction on supercoiled DNA.was investigated. The results indicated that protein fractions, of F-80 had the highest cleaving activity to supercoiled DNA.Key words : Ribosome-Inactivating Protein (RIP), supercoiled DNA , protein fraction, Morinda citrifolia.

ANTI-FUNGAL ACTIVITY OF ESSENTIAL OIL DISTILLED FROM ETHANOL EXTRACT OF Piper betle L. LEAVES COLLECTED FROM SEVERAL REGION IN YOGYAKARTA, AGAINTS Candida albicans Triana Hertiani; Indah Purwantini
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Anti-fungal activity of essential oil distilled from exctracts of Piper betle leaves against Candida albicans and the gas chromatography profiles has been studied. The leaves were collected from Kaliurang, Kulonprogo, and Gunung Kidul. This research was aimed to find out the influences of extract production process and the difference of starting material source regions to anti-fungi activities against Candida albicans. The gas chromatographic profile of essential oils distilled from sirih (Piper betle L.) extracts were also observed. The essential oils used in this research was collected from ethanol 95% extract of sirih dried leaves by distillation method. Anti-fungi activity was assayed using Sabaroud agar-diffusion method. The results showed that the essential oil from Kulonprogo has the highest activity. The gas chromatographic profile of each essential oil had been examined individually and the results showed that the composition of substances containing in each extracts were different. The study comparison of essential oils composition of Piper betle L. fresh herbs showed that the extraction method reduced the anti-candida potency of Piper betle L. leaves extract. The habitat of the starting materials influences the gas chromatographic profile of the essential oil.Key words: Piper betle Leaves Extract; anti-fungi, chromatographic profiles

CLEANING EFFECTIVITY OF SEVERAL SURFACTANS TO PESTISIDES RESIDUES ON FRESH APEL FRUITS Endang Lukitaningsih; B.S. Ari Sudarmanto; Sri Noegrohati
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Intensification efforts in farming to increase productivity must consider the pesticide utilization, especially insecticide and herbicide. Several pestisides which are still used include carbofuran and organochlorine, some of them have lipofilic properties and might harm to human health. Therefore, an effort is required to washing off pesticides from farming products is one of the effort which can be performed. Since pesticides has lipofilic properties, therefore cleaning pesticides with water is not sufficient. Surfactant is required to increase washing off ability of water. Wash off ability of several surfactants circulated on the market i. e. SL, ML and A were investigated. The result showed that the wash off ability values of surfactants to DDT residues on fresh apples were 79.18 %, 75.19 % and 67.49 % for SL, A and ML respectively. The wash off effectiveness of surfactant A, SL and ML to -metrine were 85.29 %, 80.48 % and 64.47 % respectively.Key words: pesticide, cleaning efficiency surfactant, DDT, -metrine.

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