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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
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Articles 12 Documents
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Search results for , issue "Vol. 3 No. 1 (2013)" : 12 Documents clear
BZLF1 Expression of EBV is correlated with PARP1 Regulation on Nasopharyngeal Carcinoma Tissues wahyu nur laili fajri; Ahmad Rofi'i; Fatchiyah Fatchiyah
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Nasopharyngeal carcinomas (NPC) is a cancer that arises in the epithelial tissue that covers the inside of the nasopharyngeal mucosa and nasopharynx. Infected Epstein Barr Virus (EBV) cell in a latent infection associated with the expression of nine latent proteins. Latent Membrane Protein 1 (LMP1) is one of latent proteins, and mayor EBV oncoprotein, with functions including virus growth, and to activate BamHI-Z Leftward Reading Frame 1 (BZLF1)-EBV, which can inhibit p53 to induce apoptotic resistance, metastasis, and immune modulation. The body will respond to the expansion of EBV infection with activation of Poly(ADP-ribose)Polymerase-1 (PARP1). The objective of study is to observe the expression of BZLF1 and determine PARP1 regulation in nasopharyngeal tissues. NPC-T2, NPC-T3 and polyp tissues slides are from Ulin Hospital, Banjarmasin. To characterize the necrotic cells such as pyknosis, karyorrhexsis, and karyolysis, histological slides were stained by HE that the necrotic cells measured by using a BX-53 microscope (Olympus) with CellSens Standard software. Tissues slides were stained by using immunofluorohistochemistry with EBV-BZLF1 antibody-Mouse anti-EBV monoclonal antibody against Goat anti-mouse IgG-FITC and anti-PARP1 antibody (MC-10) against Goat anti-mouse IgG labeled Rhodamin. The expression intensities were measured by Confocal Laser Scanning Microscope (Olympus). The percentage number of necrotic cells and BZLF1 and PARP1 expression intensity were analyzed using SPSS 16.0 by one-way ANOVA test with α = 0.05, beside that we use correlate and regression analyze. The research showed that the amount of karryorhexis higher than pyknosis and karyolysis in both tissues. BZLF1 expression 1.79 INT/sel (in polyp), 2.76 INT/sel (NPC Type 2) and 4.36 INT/sel (NPC Type 3), PARP1 expression 2.25 INT/sel (in polyp), 3.31 INT/sel (NPC Type 2), dan 5.93 INT/sel (NPC Type 3).The high of intensity of expression BZLF1 induced the increasing of PARP1 expression, but not correlated with percentage of necrotic cell. Interestingly, in all tissues (NPC-T1, NPC-T2 and polyp) is increased number of karyolysis cells properly, but not in pyknosis and karryorrhexis cells. The conclusion of this study indicated that the BZLF1 induce PARP1 to repair DNA damage against EBV infection.Keywords: BZLF1, CLSM, EBV, nasopharyngeal carcinoma, PARP1.
Biomass production and antibacterial activity of Justicia gendarussa Burm. f. - A valuable Medicinal plant P Sugumaran; N Kowsalya; Raju Karthic; S Seshadri
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
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Abstract

Rooting and biomass production of Justicia gendarussa has been achieved through a hydroponic system of cultivation. The obtained biomass of leaves, stem and root were examined for antibacterial activity against various human pathogenic organisms such as Staphylococcus aureus, Escherichia coli, Shigella sp., Pseudomonas sp. and Klebsiella pneumoniae. Methanolic extract of J. gendarussa root responded against E. coli. The growth of Shigella sp., Pseudomonas sp. and K. pneumonia were inhibited by leaf extract. The maximum inhibition zone against S. aureus was observed in stem extract treatment.
Survey on the use of synthetic Food Colors in Food Samples procured from different educational institutes of Karachi city. Nida Saleem; Zahida Nasreen Umar; Seema Ismat khan
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
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Abstract

The present study was carried out to find the type of food colors added to various food products especially those vended at or near different educational institutes of Karachi city. Different types of food items were analysed for isolation and identification of the added synthetic food colors. The majority of branded food items contained permitted colors however some foods manufactured locally, contained non-permitted colors. About 11% branded and 44% unbranded food items, respectively, were found with not permitted colors for human consumption. Similarly, 4% branded and 30% unbranded beverages were found unfit due to the presence of prohibited colors. Incidences of the use of non-permitted food colors were higher in case of unorganized food makers. Constant vigilance is needed to ensure that the local manufacturers comply with the regulations of food colors not only in terms of non-permitted food colors but also about the control and limits of permitted food colors. Moreover, consumption of colored food items should also be controlled by making the society aware of the hazardous effects of food colors.
The Effectivity of Scirpus Grossus and Limnocharis Flava As Fitoremediation Agents of Nitrate-Phosphate To Prevent Microcystis Blooming In Fresh Water Ecosystem Aliyah Siti Sundari; Catur Retnaningdyah; Soeharjono Soeharjono
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
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Abstract

The aims of this study were to reduce the concentration of dissolved nitrate-phosphate, furthermore it can inhibit the growth and prevent the occurrence of Microcystis bloom waters. The study was conducted experimentally in the laboratory. The Microcystis isolation carried out in Sutami reservoir. Then, remediation treatment with hydromacrophyte (Scirpus grossus, Limnocharis flava and combination of both hydromicrophyte) were done during 15 day incubation period. Abiotic factors were measured on day 0, 6, 12 and 15, but the abundance of Microcystis cells was counted daily. The productivity of hydromacrophyte was measured at the end of the research. The research results showed that hydromacrophytes were effective to reduce nitrate and phosphate concentrations. Combination of S. grossus and Microcystis reduced nitrate concentration up to 99.89 %, while highest reduction of dissolved phosphate (98.22 %) carried out by combination of L. flava and Microcystis. The combination treatment of L. flava and S. grossus was capable to prevent Microcystis growth rate and carrying capacity that is 65 cells.L-1.day-1 and 6.93 x 104 cells/mL.
Accumulation of Calcium Crystal in Rat (Rattus norvegicus) Kidney A'liyatur Rosidah; Sri Widyarti; Sri Rahayu
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
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The aim of this research is to know the effect of tuber Porang flour (Amorphophallus muelleri) to the accumulation of calcium crystal. This research uses 2,5 months old male rats that are orally given by tuber Porang flour from three different location (Klangon, Sumber Baru, and Sumber Bendo) with doses 6 mg/100 gr BW/day for 3 months. Kidneys taken to analyzed distribution of crystal calcium accumulation. Observation of distribution crystal calsium accumulation with microscope of Olympus CX 31 at 400x magnification. The data to be analysed by using test of ANOVA with SPSS 16 for windows. The result of research known that the treatment of tuber Porang flour induce calcium crystal accumulation in rat kidneys. Using tuber porang flour from different location can generate different impact at accumulation of crystal calcium. The lowest calcium crystal accumulation in kidneys with giving Klangon Porang flour that is equal to 8,19±2,17 spot/ area. Consumption of the tuber Porang flour cause the accumulation of crystal calcium in rat kidney.
Control of Microcystis spp . Bloom By Combination of Indigenous Denitrifying Bacteria From Sutami Reservoir With Fimbristylis globulosa and Vetiveria zizanoides Bayu Agung Prahardika; Catur Retnaningdyah; Suharjono -
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
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Abstract

The purpose of this research were to know the ability of polyculture macrophyte (Fimbristylis globulosa and Vetiveria zizanoides) and combination of both with consortium of indigenous denitrifying bacteria from Sutami reservoir that added by Microcystis spp. or not to reduce the concentration of nitrate, dissolved phosphate and the carrying capacity of Microcystis spp. Experiment was done in medium of Sutami reservoir water enriched with 16 ppm of nitrate and 0,4 ppm of phosphate. Denitrifying bacteria were used in this research were DR-14, DU-27-1, DU-30-1, DU-30-2, TA-8 and DU-27-4 isolated from Sutami reservoir. The treatments were incubated during 15 days. Microcystis spp. abundance was calculated every day, but measurement of the the concentration of nitrate and dissolved phosphate were done every six days. The results showed that both treatment and a combination of both macrophytes with a consortium of denitrifying indigenous bacteria were added or not either Microcystis able to reduce nitrate at 99% and 93-99% orthophosphoric. Combination of macrophytes with denitrifying indigenous bacterial consortium from Sutami reservoir was able to inhibit the carrying capacity of Microcystis spp. highest until 47.87%. They can also significantly reduce the abundance of Microcystis from 107 cells/mL in early of treatment to become 0.35x104 cells/mL after fifteen days incubation.
Observation of Frog Species in State University of Malang as a Preliminary Effort on Frog Conservation Dian Ratri Wulandari; Muhammad Habibi; Dwi Listyorini
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.3.1.%x

Abstract

Frog is an amphibian which widely distributed around the world. Indonesia houses 450 species which represent 11% of frog species in the world. In Java Island living 42 species of frogs and toads. Frogs can be used as an environment indicator due to the presence of frog in a particular place indicates that the place is stay natural and unpolluted. State University of Malang Campus #1 which is located in the heart of Malang District has been developing rapidly, currently. Thus, it requires for the construction of new various facilities to support it widen activities. Extensive construction can be destructive even damaging to the habitat of frog, which potentially threat the frog life, if it does not take a consideration on the environmental impact, carefully. This study aimed to identify the species of frog which is survives in State University of Malang with consideration of the frog species found in 1995. Species identification conducted by observing the morphologicalcharacter. This study found four species with three species which are survive and as found in 1995; those are Duttaphrynus melanostictus, Polypedates leucomystax, and Kaloula baleta; and one new species that is Rana chalconota. This study also reveals there are four species which are extinct; those are Fejervarya cancrivora, Fejervarya limnocharis, Ingerophrynus biporcatus, and Occidoziga lima. This situation shows the decreasing amount of species from 7 to 4 within 17 years. This result indicates that there is a serious environmental degradation which caused the losing of frog habitats. Further research is needed to study the ecological condition changing in order to save the frog species.
The Technic of Agroforestry at Communal Forest Areas in Pejarakan Village, District of Gerokgak, Regency of Buleleng, Province of Bali I putu Gede Ardhana; Sunardi Sunardi
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
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Abstract

Since 2003 year the technic of agroforestry with alley cropping systems has been implemented at the forest areas in Pejarakan Village, District Gerokgak, Regency of Buleleng Province of Bali. These activities are expected to support the progress of welfare for farmers, eventhough they have been neglected to preserve forest around village. After the research it was cleared that the result of activities didnt fulfilled to its expectation. The analysis about the compositions and structures of vegetations in this research shows that the dominant type of species which are mainly cultivated have character of Importance Value Index, the balance of species among Diversity Index are still low at all of the communal forest areas and even at the these slope sides. The composition and the structure of species were still few, and the vegetation itself was disturbed by people, in order to illegal loggings, cuttings top branches or twigs often occur, and these activities bring out obstacle growth of trees in communal forest areas. It is concluded that the technic of agroforestry at the communal forest areas has few compositions of species and structure of vegetations, so the diversity of species become very poor and not stable either for plants in forestry or for agricultural crops. The researcher could not determine that what species of vegetations superior at this area. So it necessary to enough time for intensive observations and research at this communal forest areas.
Alteration of Coral Reef Community Structure Caused by Athropogenic Activity in Coastal Area of Giliketapang Island, Probolinggo, East Java, Indonesia Lutfiatul Puspitasari; Setijono Samino; Catur Retnaningdyah
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
Publisher : Journal of Tropical Life Science

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The aim of this research was to know the effect of anthropogenic activity around the coastal Giliketapang Island to community structure of coral reef. This research used belt transect method which consist of 18 transects in three location (harbour,near the forest and transition areas)at 50-80 m from the coast, with plot size of 2x2m2. Abundance of coral reefs were observed base on the coverage. There are found 11 families of coral reefs. The percentage of coral damage was decrease and the number of species was increase at the far zone from coast. This is also affect the coverage value of coral reefs. Coral coverage at the harbour and near the forest was 53. 06 % and 65. 36 % respectively, whereas in the transition area was 38. 38%. Co-domination between Porites lobata and Chyphastrea microphthalma occurred at the harbour, while Porites mayeri and Acropora spp. co-dominate in near the forest location. Poritidae family dominate in the transition area. Anthropogenic activities at transition location cause severed damage of coral reefs.
Exploratory Evaluation of Retranslocation and Bioconcentration of Heavy Metals in Three Species of Mangrove at Las Cucharillas Marsh, Puerto Rico Carla Lorraine Mejías; Juan Carlos Musa; Juan Otero
Journal of Tropical Life Science Vol. 3 No. 1 (2013)
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Abstract

Heavy metal contamination in the coastal area of Cataño, Puerto Rico accountable to anthropogenic sources is of great concern due to the risk posed over the surrounding communities and adjacent ecosystems. Estuarine ecosystems are widely recognized for the presence of mangroves. This type of flora is recognized for their many beneficial properties for example, the ability to purge aquatic ecosystems where they stand. Exploratory analyses on the phytoaccumulative capacity of three mangrove species for ten metals (Hg, Al, As, Cd, Cr, Cu, Fe, Pb, Mg, Zn) were performed in this research. Random soil samples and both green and senescent leaves of Rhizophora mangle, Laguncularia racemosa and Avicennia germinans from three different sites of Peninsula La Esperanza were analyzed in order to apply the retranslocation efficiency (RT%) and bioconcentration factor (BCF) concepts. After calculating the RT% and the BCF, comparison analyses among the three mangrove species were performed. In general, the results showed low RT% values for Avicennia in comparison with Rhizophora and Laguncularia. BCF values confirmed RT% results for Avicennia, showing higher heavy metal concentrations in its senescent leaves in contrast with the other species. Therefore, these preliminary results suggest that Rhizophora and Laguncularia act better as phytoremediators for heavy metals in polluted areas due to their ability to accumulate lower concentrations in senescent leaves; preventing further contamination in surrounding ecosystems by encapsulating the pollutants instead of exporting them.

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