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All Journal JBIO: jurnal biosains (the journal of biosciences) Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Saintia Biologi JURNAL PENELITIAN SAINTIKA JURNAL PENGABDIAN KEPADA MASYARAKAT Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Chemistry Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Pembelajaran dan Biologi Nukleus KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Isnaini Nurwahyuni
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Indonesian Journal of Chemistry

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The Development of Reproducible and Selective Uric Acid Biosensor by Using Electrodeposited Polytyramine as Matrix Polymer Manihar Situmorang; Isnaini Nurwahyuni
Indonesian Journal of Chemistry Vol 17, No 3 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.143 KB) | DOI: 10.22146/ijc.25818

Abstract

A versatile method for the construction of reproducible and high selective uric acid biosensor is explained. Electrodeposited polytyramine is used as biosensor matrixes due to its compatibility to immobilize enzyme uric oxidase in the membrane electrode. The precise control over the charge passed during deposition of polytyramine allows concomitant control over the thickness of the deposited enzyme layers onto the surface of the electrode. The uric acid biosensor showed a sensitive response to uric acid with a linear calibration curve lies in the concentration range of 0.1–2.5 mM, slope 0.066 µA mM-1, and the limit detection was 0.01 mM uric acid (S/N = 3). The biosensor shown excellent reproducibility, the variation between response curves for uric acid lies between RSD 1% at low concentrations and up to RSD 6% at saturation concentration. Uric acid biosensor is free from normal interference. The biosensor showed good stability and to be applicable to determine uric acid in real samples. Analysis of uric acid in the reference standard serum samples by the biosensor method are all agreed with the real value from supplier. Standard samples were also analyzed independently by two methods: the present biosensor method and the standard UV-Vis spectrophotometry method, gave a correlation coefficient of 0.994. This result confirms that the biosensor method meets the rigid demands expected for uric acid in real samples.
Co-Authors . Rosmayati ., Rosmayati Andini - Hanif Arifda Ayu Deny Supriharti Dessy Merry Silitonga DWI SURYANTO Elimasni Elimasni Fathurrahman Fathurrahman Fathurrahman Fathurrahman Fauziah Harahap Fauziah Harahap Hasianna Nopina Situmorang Justin A. Napitupulu manihar Situmorang, manihar Mayang Sari Yeanny Mizawarti Mizawarti Napitupulu, Justin A Nunuk Priyani Radite Tistama Rahma Dina Rani Ernita Limbong Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Silitonga, P Maulim Sinaga, Rianto Sinaga, Riyanto Siregar, Linda Sari Steven Taniwan Vahnoni Lubis Wilander Simarmata Yurnaliza, Yurnaliza