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All Journal JBIO: jurnal biosains (the journal of biosciences) Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Saintia Biologi JURNAL PENELITIAN SAINTIKA JURNAL PENGABDIAN KEPADA MASYARAKAT Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Chemistry Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Pembelajaran dan Biologi Nukleus KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Isnaini Nurwahyuni
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Saintia Biologi

 1 
PEMANFAATAN BAKTERI KITINOLITIK DALAM MENGHAMBAT PERTUMBUHAN Curvularia sp. PENYEBAB PENYAKIT BERCAK DAUN PADA TANAMAN MENTIMUN Andini - Hanif; Dwi Suryanto; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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A study on about the utilization of chitinolytic bacterial isolates to inhibit growth of Curvularia sp. causal agent of leaf spot disease of cucumber was done in Laboratory of Pest and Disease, Medan Johor, UPT-Balai Protection Plant and Horticulture I and Laboratory Microbiology Departement of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan. Six chitinolytic isolates were tested in vitro to inhibit growth of Curvularia sp.. The result showed that Bacillus sp. BK13 inhibited more with inhibition zone of 2,75 cm and Enterobacter sp. BK15 with inhibition zone of 2,55 cm, whereas Enterobacter sp. PB17 showed the lowest inhibition zone of 1,3 cm. Soaking seed treatment in chitinolytic bacterial suspension reduced the percentage of leaf spot. Enterobacter sp. BK15 showed more ability (50%) to inhibit leaf spot attack.   Keywords: Bacillus sp. BK13, cucumber, Curvularia sp., Enterobacter sp. BK15, leaf spot
POTENSI BAKTERI KITINOLITIK DALAM PENGENDALIAN Aspergillus niger PENYEBAB PENYAKIT BUSUK PANGKAL AKAR PADA TANAMAN KACANG TANAH Arifda Ayu; Dwi Suryanto; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

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A study of chitinolytic bacteria ability to control Aspergillus niger, a causal agent of basal root rot of peanut seedlings was conducted in Pest and Disease Laboratory, Medan Johor and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan.  The purpose of study is to evaluate the ability of Bacillus sp. BK13, Enterobacter sp. BK15, Bacillus sp. BK17, Enterobacter cloacae LK08, Bacillus sp. KR05, and Enterobacter sp. PB17 to inhibit the growth of A. niger on the peanut seedling.  Antagonistic test showed that the most effective bacteria in inhibiting the growth of A. niger was BK15 isolate with inhibition zone of 2,88 cm and BK13 isolate with inhibition zone of 2,69 cm, whereas the least effective bacteria was BK17, with inhibition zone of 2,30 cm. Chitinolytic bacterial isolates used to cover peanut seed through soaking enabled to reduce seed basal root rot. BK15 isolate showed potential to reduce the basal root rot by 58,82% while the lowest inhibition was BK13 by 47,06%.   Keywords: Aspergillus niger, Bacillus sp., chitinolytic bacteria, Enterobacter sp., peanut
ISOLASI DAN UJI POTENSI ISOLAT BAKTERI PELARUT FOSFAT DAN BAKTERI PENGHASIL HORMON IAA (Indole Acetic Acid) TERHADAP PERTUMBUHAN KEDELAI (Glycine max L.) PADA TANAH KUNING Dessy Merry Silitonga; Nunuk Priyani; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

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Biofertilizer is a safe alternative fertilizer instead of various chemical fertilizers for increasing plant productivity which can minimize the ecological damage. There are plant symbiotic-soil bacteria which can solubilize phosphate or produce IAA. The objectives of this research are to determine the best soil bacteria in producing IAA or solubilizing phosphate and to know their roles in promoting plant growth in unfertile soil. As many as 8 isolates of phosphate solubilizing bacteria and 5 isolates of IAA producing bacteria were found. Among those isolates, there were 2 potential isolates (P2 & P4) that were able to solubilize phosphate and 1 potential isolate (I3) that was able to produce IAA. Isolate P4 produced holozone as wide as 1.45 cm and isolate P2 was 1.3 cm. Meanwhile, isolate I3 produced the highest concentration of IAA compared to the other isolates, that was 33.3 ppm. These isolates were applicated further on unfertile soil (yellow soil) as a medium for soybean growth. Soybeans have been grown for 10 weeks to observe the effect of those potential isolates on plant growth and productivity. The result showed that application of  isolate I3 was able to promote plant growth and increase plant productivity better than the other isolates as well as controls (fertile and unfertile soil). It promoted the soybean growth such as plant height was 58,8 cm; plant fresh weight was 3.5 g; plant dry weight was 1.55; pod fresh weight was 1.23; pod dry weight was 1.03; the amount of the pod was 3 and the number of the seed was 7.   Keywords : Phosphate solubilizing bacteria, IAA producing bacteria, biofertilizer, soybean.
ANALISIS KARIOTIPE DAN PERTUMBUHAN KACANG KEDELAI (Glycine max L.) SETELAH DIINDUKSI SINAR ULTRAVIOLET Rani Ernita Limbong; Elimasni D; Isnaini Nurwahyuni
Saintia Biologi Vol 1, No 3 (2013)
Publisher : Saintia Biologi

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“Karyotype and Growth Analysis of Soybean (Glycine max L.) after Exposed by Ultraviolet light” has been done at Genetic and Molecular Biology Laboratory at Natural Scienes Faculty and the greenhouse at Agriculture Faculty Sumatera Utara University Medan. The objective was to investigate design karyotype and vegetative growth of soybean that induced by UV light. The experimental design was used complete factorial randomized with 2 factors. The first are Ultraviolet (UV) light 3 levels : 10, 20 and 30 Watt and the second is the time of UV exposure with 3 levels : 5, 10, and 15 minutes. Each treatment replicates 2 times and plant that not exposed by UV used as control. The karyotype was observed by squash method. The results showed that the intensity and duration of UV light have no effect on the number of chromosomes but the light has effect on the number of chromosome type. Statistical analysis showed that the increase in UV energy up to 30 Watt and time exposure of 15 minutes, caused the increase in height, number of leaves, number of flowers, the fresh and dry weight of the plant, and chlorophyll concentration compared to untreated plants. Keywords: karyotype, mutation, soybean, ultraviolet.
Co-Authors . Rosmayati ., Rosmayati Andini - Hanif Arifda Ayu Deny Supriharti Dessy Merry Silitonga DWI SURYANTO Elimasni Elimasni Fathurrahman Fathurrahman Fathurrahman Fathurrahman Fauziah Harahap Fauziah Harahap Hasianna Nopina Situmorang Justin A. Napitupulu manihar Situmorang, manihar Mayang Sari Yeanny Mizawarti Mizawarti Napitupulu, Justin A Nunuk Priyani Radite Tistama Rahma Dina Rani Ernita Limbong Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Silitonga, P Maulim Sinaga, Rianto Sinaga, Riyanto Siregar, Linda Sari Steven Taniwan Vahnoni Lubis Wilander Simarmata Yurnaliza, Yurnaliza