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All Journal JBIO: jurnal biosains (the journal of biosciences) Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Saintia Biologi JURNAL PENELITIAN SAINTIKA JURNAL PENGABDIAN KEPADA MASYARAKAT Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Chemistry Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Pembelajaran dan Biologi Nukleus KLOROFIL: Jurnal Ilmu Biologi dan Terapan
Isnaini Nurwahyuni
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara, Medan, Sumatera Utara, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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Journal : JURNAL PENELITIAN SAINTIKA

 1 
PENGEMBANGAN METODE ANALISIS SPEKTROFOTOMETRY UNTUK PENENTUAN KOLESTEROL DI DALAM MAKANAN TRADISIONAL Situmorang, Manihar; Silitonga, P Maulim; Nurwahyuni, Isnaini; Siregar, Linda Sari
JURNAL PENELITIAN SAINTIKA Vol 12, No 2 (2012): SEPTEMBER 2012
Publisher : JURNAL PENELITIAN SAINTIKA

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Abstract

The development of analytical UV-Vis spectrophotometric method forthe determination of cholesterol is explained. The assay performed by enzymaticcatalytic reaction ·using cholesterol oxidase (COx) to . oxidise cholesterol into cholestenon and hydrogen peroxide followed by catalytic reduction of hydrogenperoxide with o-dianicidine by using enzyme peroxidase (POx) to produce red colour reduced o-dianicideine that can be measured at ii. 520 nm. The modifiedmethod gave sensitive response to cholesterol, where the detection linearity to cholesterol lies between 0.01-5.0 mM cholesterol, slope 0.158 aulmM cholesterol,and the detection limit of 0~01 mM cholesterol. The method has been applied for thedetermination of cholesterol in traditional food samples, where the concentrations ofcholesterol in food are lies between 0.052-0.799 mM cholestero)
PERTUMBUHAN OKULASI JERUK KEPROK BRASTEPU (Citrus nobilis Var. Brastepu) MENGGUNAKAN JERUK ASAM SEBAGAI BA TANG BA WAH Nurwahyuni, Isnaini; Napitupulu, Justin A; ., Rosmayati; Harahap, Fauziah
JURNAL PENELITIAN SAINTIKA Vol 12, No 1 (2012): MARET 2012
Publisher : JURNAL PENELITIAN SAINTIKA

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The growth of Citrus nobilis Var. Brastepu budding that is free fromCitrus Vein Phloem Degeneration (CVPD) is explained. The method is conductedby taking of bud stick from a selected healthy citrus Brastepu plant and is thengrafted into a sour orange (Citrus auraniium) as mother trees via budding method.The research is conduted in Desa Bukit, Brastagi North Sumatra, Indonesia.Optimum conditions on the budding technique have been obtained to produce goodqualih; seedling of Citrus nobilis Var. Brastepu. Growth characteristics of the plantin various treatment conditions have been studied based on the growth leaves, stem,and branches. Analysis of the DNA using RAPD-PCR method has confirmed thatall new seedling produced in the propagation method are free from CVPD.
Co-Authors . Rosmayati ., Rosmayati Andini - Hanif Arifda Ayu Deny Supriharti Dessy Merry Silitonga DWI SURYANTO Elimasni Elimasni Fathurrahman Fathurrahman Fathurrahman Fathurrahman Fauziah Harahap Fauziah Harahap Hasianna Nopina Situmorang Justin A. Napitupulu manihar Situmorang, manihar Mayang Sari Yeanny Mizawarti Mizawarti Napitupulu, Justin A Nunuk Priyani Radite Tistama Rahma Dina Rani Ernita Limbong Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Rizky Yudha Pratama Silitonga, P Maulim Sinaga, Rianto Sinaga, Riyanto Siregar, Linda Sari Steven Taniwan Vahnoni Lubis Wilander Simarmata Yurnaliza, Yurnaliza