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All Journal Jurnal Kedokteran Syiah Kuala Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Pharmaceutical and Clinical Research International Journal Of Science, Technology & Management (IJSTM)
Hasibuan, Poppy Anjelisa Zaitun
Universitas Sumatera Utara
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Antioxidant Activity of n-Hexane, Ethyl Acetate and Ethanol Extract from Lakoocha Leaves (Artocarpus lacucha Buch.-Ham) using DPPH Method Zaitun Hasibuan, Poppy Anjelisa; Mardiana
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 1 No. 2 (2018): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (838.267 KB) | DOI: 10.32734/idjpcr.v1i2.433

Abstract

This study aimed to investigate phytochemical screening and antioxidant activity of n–hexane, ethyl acetate and ethanol extract from lakoocha leaves. The powdered simplicia was macerated with n–hexane, ethyl acetate and ethanol 96% successively, filtered, then concentrated using rotary evaporator to obtain n–hexane extract, ethyl acetate extract and ethanol extract. Phytochemical screening and antioxidant activity was performed against these extracts. Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method using ultraviolet-visible spectrophotometer at wavelength of 516 nm after incubated for 60 minutes in dark place. Quercetin was used as positive control. The result of phytochemical screening showed n-hexane extract contains steroid, ethyl acetate extract contain steroid, tannin, glycoside, flavonoid and saponin, whereas ethanol extract contain tannin, glycoside, flavonoid and saponin. The IC50 value of n–hexane, ethyl acetate and ethanol extract was 1062.03±1.42 ppm, 323.18±0.02 ppm and 99.23±0.07 ppm respectively, whereas for quercetin was 2.32±0.01 ppm. This study showed that ethanol extract had antioxidant activity with strong category whereas n-hexane extract and ethyl acetate extract had inactive antioxidant activity with very weak categories. Keyword: Antioxidant Activity, DPPH, Lakoocha leaf
Cytotoxicity Activity of Ethanol Extract of Andaliman Fruits (Zanthoxylum acanthopodium DC.) towards 4T1 Breast Cancer Cells Rosidah, Rosidah; Hasibuan, Poppy Anjelisa Zaitun; Haro, Ginda; Satria, Denny
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 2 No. 2 (2019): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (888.661 KB) | DOI: 10.32734/idjpcr.v2i2.3220

Abstract

Breast cancer is one of the world's leading cause of death in women. Due to the resistance of chemotherapeutic agents, there is a continuous need to search of natural products with anticancer activity. The use of natural products is expected to increase the effectiveness and decrease side effect. The purpose of this study was to investigate the anticancer activity of ethanol extract of andaliman fruits (EEAF) towards 4T1 cells. Extracts were prepared by maceration using solvent ethanol 96%. 4T1 cells were grown in culture medium DMEM then given by EEAF and doxorubicin. Cytotoxic test in vitro was done by MTT method [3-(4,5-dimetiltiazol-2-il) -2.5 difeniltetrazolium bromide] which is then analyzed using SPSS 21. The results from this study showed that the cytotoxic results (IC50) after treatment with EEAF and doxorubicin were 54.48 ± 0.22 µg/mL dan 0.80 ± 0.02 µg/mL.Based on the result above, we conclude that EEAF has cytotoxic activity towards 4T1 cancer cells. Key words: andaliman fruits, Zanthoxylum acanthopodium DC., ethanol extract, breast cancer, 4T1 cell line.
Effect of Plectranthus amboinicus, Lour. Spreng. n-Hexane Extract on T47D Cells Line: Proliferation, Apoptosis and It’s Combination with Doxorubicine Zaitun Hasibuan, Poppy Anjelisa; Rosidah; Nasution , Pandapotan; Ilyas , Syafruddin
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 3 No. 1 (2020): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/idjpcr.v3i1.3323

Abstract

The objectives of the study are to investigated the growth-inhibiting and apoptosis mediating effect of Plectranthus amboinicus (Lour.) Spreng n-hexane extract (PANE) on T47D cell lines. The assays were performed in the study were cytotoxicity assay and apoptosis induction of T47D cells. The cytotoxicity effects were determined by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The effect of apoptosis were observed by ethidium bromide-acrydine orange method. The growth of T47D was inhibited by treatment with PANE on concentrations 20; 40; dan 80 µg/mL. The cell death (apoptosis) induced by PANE was characterized by orange fluorescent on the IC50 concentration. These results concluded that the n-hexane extract of Plectranthus amboinicus, (Lour.) Spreng. inhibited the growth of T47D breast cancer cells in dose and time dependent manners. PANE also could induced apoptosis on T47D breast cancer cells, but the combination of PANE-doxorubicine did not show the synergistic effect. The results suggesting that PANE may be a potent agent for the chemoprevention of breast cancer.
Effect Of Bangun-Bangun Leaves Ethanol Extract On Lipid Profile Ovariectomized Female Rats Suharyanisa; Hasibuan, Poppy Anjelisa Zaitun; Nasution, Pandapotan
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 4 No. 1 (2021): Indonesian Journal of Pharmaceutical and Clinical Research
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/idjpcr.v4i1.5360

Abstract

Menopause was a condition of the body when ovaries stopped producing ovum; ovum is the main producer of estrogen hormone. Menopause can increasing lipid profile in blood; it causes any problems in the cardiovascular system. People of North Sumatra have long used Bangun-Bangun leaves as a breastfeeding agent; it contains flavonoid bioactive compounds, which are be expected to replace lost estrogen hormone. This study determined the estrogenic activity of Bangun-Bangun leaves ethanol extract (BBLEE) on lipid levels of ovariectomized female rats as a menopausal model. BBLEE has obtained by maceration techniques with 96% ethanol and extract used on 24 ovariectomized rats which were divided into 6 groups. Group 1 (normal), group 2 (positive) was treated by estradiol at dose of 0.18 mg/kg BW, group 3 (negative) was treated by 0.5% Na-CMC and group 4, 5, 6 were treated by BBLEE at dose of 30, 60, 90 mg/kg BW given orally for 14 days. The data were analyzed by ANOVA and Post Hoc Tukey HSD test. The result showed that effective dose of BBLEE was 90 mg/kg BW which improved lipid profile (TC = 55.75±0.47; TG = 63.00±0.40; HDL = 37.00±0.81; LDL = 30.50±0.86) in blood of ovariectomized female rats as a menopausal model. BBLEE at the dose of 90 mg/kg BW can ameliorate the lipid profile in the blood of ovariectomized female rats as a menopausal model.
Uji sitotoksik metformin hidroklorida terhadap sel kanker payudara T47D Irma Yanti Rangkuti; Poppy Anjelisa Zaitun Hasibuan; Tri Widyawati; M. Ichwan
Jurnal Kedokteran Syiah Kuala Vol 19, No 3 (2019): Volume 19 Nomor 3 Desember 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v19i3.18112

Abstract

Abstrak. Latar Belakang. Kanker payudara merupakan penyakit berlebihnya pertumbuhan atau tidak terkendalinya perkembangan sel kanker payudara. Kanker merupakan suatu penyakit yang disebabkan kelainan genetik berupa mutasi DNA yang menyebakan hilangnya kontrol pertumbuhan. Gangguan genetik ini menyebabkan terganggunya siklus sel dan apoptosis. Metformin merupakan suatu antihiperglikemik yang digunakan pada pasien diabetes melitus tipe 2. Penurunan risiko kanker terjadi pada pasien diabetes melitus tipe 2 yang menggunakan metformin. Uji sitotoksik untuk agen anti kanker merupakan uji skrining awal untuk menilai potensi efek anti kanker. Tujuan penelitian ini adalah untuk mengetahui efek sitotoksik metformin hidroklorida terhadap pertumbuhan sel kanker payudara T47D. Metode: Penelitian ini merupakan penelitian eksperimental uji invitro terhadap sel kanker payudara T47D yang dipaparkan metformin HCl konsentrasi 5000; 2500; 1250; 312.5 dan 156,25 μM selama 24 jam. Sebagai pembanding digunakan paclitaxel konsentrasi 1000; 500; 250; 31,25 dan 15,625 nM. Uji sitotoksik menggunakan metode MTT untuk menentukan IC50.Data dianalaisis menggunakan analisa probit. Hasil : IC50  metformin HCl adalah 13457.3 ± 1096,5 μM. IC50 paclitaxel adalah 1577.2 ± 115.3 nM. Efek anti kanker metformin lebih kecil dibanding paclitaxel. Kata Kunci: Metformin HCl, T47D, uji sitotoksik, IC50 Abstract. Breast cancer is a disease in which there is excessive growth or uncontrolled development of breast tissue cells. Cancer is a disease caused by genetic disorders caused by DNA mutations that cause loss of growth control. This genetic disorder affects the cell cycle and cell apoptosis and causes the formation of cancer. Metformin is an antihyperglycemic in type 2 diabetes mellitus patient. The decrease in cancer risk occured in patients with type 2 diabetes mellitus who used metformin. Cytotoxic test for agent anti cancer  is screening test to investigate the potency cancer effect of substance. The goal of this study was determining the cytotoxic effect of metformin hydrochloride to T47D breast cancer cell. The method : This sudy was experimental study, invitro test to T47D breast cancer cell using metformin HCl 5000; 2500; 1250; 312.5; and 156.25 μM for 24 hours. Paclitaxel used as postiive control with concentration were 1000; 500; 250; 31,25 and 15,625 nM. Cytotoxic test using MTT method to determine IC50. Data were analyzed using probit analysis using SPSS 22 version. The result of cytotoxic test showed that IC50 metformin HCl was 13457.3 ± 1096,5 μM. While IC50 paclitaxel as control was 1577.2 ± 115.3 nM. The effect of cancer metformin HCl was lower than paclitaxel.Keywords: Metformin HCl, T47D, cytotoxic test, IC50
Total Phenolic Content of Ethanol Extract of Artrocarpus camansi Leave and its Effect to SOD (Superoxide Dismutase) Level in Mice Hendri Asrin; Poppy Anjelisa Zaitun Hasibuan; Marianne Marianne
Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss3pp101-109

Abstract

A free radical is one of the triggers of degenerative diseases that become the biggest cause of death. Excessive production of free radicals can be neutralized by antioxidant. Antioxidants can be generated from within the body (intracellular). One of them by the enzyme SOD (superoxide dismutase). However, when the production of free radicals exceeds the ability of intracellular antioxidants to neutralize it, antioxidants from outside (extracellular) is necessary. The ethanol extract of Artocarpus camansi leaves (EEACL) contains phenolic compounds which has very strong antioxidant activity based on in vitro study using the DPPH method, but the in vivo study about the total phenolic content effect of its leaves toward antioxidant activity has not been done. 25 mice were divided into 5 groups consisting of control group, a group was induced by stress and three groups were induced by stress, but given EEACL with each dose of 50 mg/kg bw, 100 mg/kg bw and 150 mg/kg bw. Inducing stress in the form of psychological stress was carried out for 7 days and continued with the EEACL administration for 7 days. The mice were dissected and the livers were isolated, then the liver morphological was examined using Hematoxyllin Eosin (HE) staining method and SOD level was examined with immunohistochemical staining method. The data were analyzed with One Way ANOVA and Duncan test using SPSS program version 19.0. Total phenolic content of EEACL is 235.03 ± 4.306 mg GAE/ g of sample. The average SOD levels in the control group is 94.05 %, stressed group is 55.94 %, stress with EEACL dose 50 mg/kg bw group is 58.40 %, stress with EEACL dose 100 mg/kg bw group is 79.68 %, stress with EEACL dose 150 mg/kg bw group is 80.90 %. Based on statistical result, SOD level increased along with the increase of EEACL dose, but not significantly (p < 0,05). Total phenolic content of EEACL has an influence to SOD levels. SOD level increased along with the increase of EEACL administration dose. The higher dose of EEACL leading to higher levels of SOD in the mouse liver.Keywords : total phenolic content, superoxide dismutase, antioxidant, Artocarpus camansi
Estrogenic Activity of Mahoni Seed Ethanolic Extract [Swietenia mahogany (L.) Jacq] on Uterus Weight, Bone Density and Mamae Gland Proliferation on Ovariectomized Rats Poppy Anjelisa Zaitun Hasibuan; Rosa Gloria Sitanggang; Robbani Syahfitri Angkat
Indonesian Journal of Cancer Chemoprevention Vol 11, No 2 (2020)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev11iss2pp75-83

Abstract

Menopause is a hypoestrogenic condition due to decreased function of the ovary. During menopause there is no reserved ovum in the ovary, as a result the synthesis of estrogen by the follicles does not take place. Deficiency of estrogen can lead to discomfort and decrease in the women quality of life. Therefore, supplements from natural resources to reduce menopausal symptoms will be needed. The objectives of the study were to determine the effect of mahogany seeds ethanolic extract (MSEE) on the development of uterus, bone density, and mammae gland proliferation on ovariectomized rats. Extract was made by maceration using 96% ethanol as the solvent, then the study of estrogenic effect was carried out on 30 female rats which were divided into 6 groups. Group 1 (normal control), group 2 (positive control) given estradiol dose of 0.18 mg/kg body weight (BW), group 3 (negative control) given Na-CMC 1% and group 4, 5, 6 given MSEE orally for 14 consecutive days with doses of 50, 100, 200 mg/kg BW. Data were analysed using ANOVA then continued with Tukey HSD Post Hoc test to see the differences between the treatments. The results of the study showed that MSEE was able to increase the weight of the uterus, the length of estrus phase in the estrus cycle, bone density and the mammae gland proliferation of rats. The results concluded that MSEE has phytoestrogenic effect on ovariectomized rats.Keywords: phytoestrogen, ovariectomy, uterus weight, bone density, mammae proliferation
Antioxidant Properties and Cytotoxic Activity of Ethyl Acetate Fraction of Plectranthus amboinicus (Lour.) Spreng. Leaves on HeLa and T47D Cell Lines Poppy Anjelisa Zaitun Hasibuan; Panal Sitorus; Denny Satria; Rizka Damela Sibuea
Indonesian Journal of Cancer Chemoprevention Vol 10, No 1 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss1pp37-45

Abstract

Research into plants with anticancer effects is actively encouraged in orderto discover new drugs with lessertoxicity but more potent effects. The aims of study are to evaluate the antioxidant properties and to investigate the cytotoxic activity of Plectranthus amboinicus (Lour.) Spreng. leaves ethyl acetate fractions on HeLa,T47D and MCF7 cell lines. The extract was prepared by graded maceration using n-hexane and ethyl acetate. The ethyl acetate extract was fractionated in vacuum liquid chromatography with n-hexane: ethyl acetate; and ethyl acetate: methanol as mobile phase. Then, the fractions were analyzed with thin layer chromatography (TLC). The free radical scavenging activity was measured by DPPH method, the total flavonoid content was calculated by quercetin equivalent and the absorbance is measured by using UV-Visible spectrophotometry. The cytotoxic activity were determined using MTT assay. The fractions contained 5 sub fractions with same TLC profile. The fractions showed antioxidant activity by DPPH method with different IC50 values, namely: 130 µg/mL(I), 127 µg/mL(II), 137 µg/mL(III), 129 µg/mL(IV), and 124 µg/ mL(V), respectively. The measurement of total flavonoid content showed 118 mg QE/g (I), 50 mg QE/g (II), 207 mg QE/g (III), 56 mg QE/g (IV), and 55 mg QE/g (V). The IC50 of each sub fractions on HeLa cell were 77 µg/mL, 46 µg/mL, 93 µg/mL, 71 µg/mL and 476 µg/mL; for T47D cell were 1621 µg/mL, 111 µg/mL, 128 µg/mL, 150 µg/mL and 209 µg/mL; and for MCF7 were 259 µg/mL, 343 µg/mL, 575 µg/mL, 408 µg/mL and 250 µg/mL. Based on the results, the fractions derived from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. leaves exhibit antioxidant. The Fraction II from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. was the most cytotoxic on HeLa, T47D and MCF7 cell lines. It is potential to undergo further isolation of its cytotoxic compounds.Keywords : antioxidant, cytotoxic, Plectranthus amboinicul (Lour.) Spreng., ethyl acetate fractions
Formulation and Evaluation of Liposome Moringa Oleifera Seed Oil (Moringa oleifera L.) as Anti-aging Putri Tri Hartini; Sumaiyah; Poppy Anjelisa Zaitun Hasibuan
International Journal of Science, Technology & Management Vol. 3 No. 4 (2022): July 2022
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v3i4.546

Abstract

Moringa oil is a natural vegetable oil obtained from the juice of the ripe kernels of the Moringa tree (Moringa oleifera L). Moringa seeds have an oil content of up to 40%, and have antioxidant activity with vitamin E content reaching 51% but have low stability. The purpose of this study was to find the best formulation as an anti-aging on liposome delivery system with a ratio of phosphatidylcholine soy lechitin and cholesterol. The method used is thin layer hydration (Thin Film Hydration). The results of the observation on the best formula are the 6th formula on organoleptic examination which is milky yellow in color, has a distinctive smell of lecithin and cholesterol and is in the form of a thick suspension, with a pH of 6.0, particle size 83.98nm using a particle size analyzer and entrapment efficiency of 96.8 % using a UV-Vis spectrophotometer.
Antibacterial Activity of Patch Silver Nanoparticles and Chitosan with Cellulose Nanofibers Carriers against Staphylococcus aureus and Escherichia coli Andre Prayoga; Poppy Anjelisa Zaitun Hasibuan; Yuandani
Indonesian Journal of Pharmaceutical and Clinical Research Vol. 4 No. 2 (2021): Indonesian Journal of Pharmaceutical and Clinical Research (IDJPCR) Vol. 04, No
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/idjpcr.v4i2.6169

Abstract

One of the medical needs whose demand continues to increase is wound dressings. The wound cover must also be non-toxic, non-allergenic, made of widely available biomaterials, and have antibacterial properties that can prevent infection of the wound. Chitosan is known to have wound healing activity by acting as a blood-clotting agent and stimulating the formation of new tissue, and silver nanoparticles have good antibacterial activity. Silver Nanoparticles and Chitosan with Cellulose Nanofibers carriers (SNCCN) are made in the form of patches with the ratio formula between cellulose nanofibers and chitosan/silver nanoparticles is 1:9, 2:8, 3:8, 4:7, 5:5, 6:4, 7:3, 8:2, 9:1, and 10:0. Then the antibacterial activity was tested against Staphylococcus aureus and Escherichia coli to find the best formula for antibacterial activity. The analysis showed that the SNCCN patch with a ratio of 9:1 had the best antibacterial activity against Staphylococcus aureus (13.8±0.05 mm) and Escherichia coli (12.5±0.05 mm). It can be concluded that patch Silver Nanoparticles and Chitosan with Cellulose Nanofibers carriers (SNCCN) have good antibacterial activity at a concentration of 9:1 in the category of strong inhibition (10-20 mm).
Co-Authors Andre Prayoga Denny Satria Denny Satria, Denny Haro, Ginda Hendri Asrin Ilyas , Syafruddin Irma Yanti Rangkuti Junius Gian Ginting M. Ichwan Mardiana Marianne Marianne Nasution , Pandapotan Nasution, Pandapotan Panal Sitorus Putri Tri Hartini Rizka Damela Sibuea Robbani Syahfitri Angkat Rosa Gloria Sitanggang Rosidah Rosidah Rosidah Suharyanisa Sumaiyah Tri Widyawati Yuandani Yuandani