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All Journal Indonesian Journal of Cancer Chemoprevention Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat Medical Sains : Jurnal Ilmiah Kefarmasian
Aji Winanta
Fakultas Kedokteran Dan Ilmu Kesehatan, Prodi Farmasi, Universitas Muhammadiyah Yogyakarta, Jln. Brawijaya, Kasihan, Bantul, Yogyakarta 55183.
Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Medicine & Pharmacology Social Sciences

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Journal : Indonesian Journal of Cancer Chemoprevention

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Antioxidant Activity and Cytotoxic Potential of Parijoto (Medinilla speciosa (Reinw ex BL)) Fruit Fractions on HeLa Cell Line Aji Winanta; Linta Sabila Hanik; Rifki Febriansah
Indonesian Journal of Cancer Chemoprevention Vol 12, No 2 (2021)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev12iss2pp74-82

Abstract

Parijoto (Medinilla speciosa (Reinw ex BL)) is one of the Indonesian indigenous plants widely used as traditional medicine. A previous study showed that ethanol and methanol extracts of Parijoto fruit could inhibit T47D breast cancer cells. This study explores the antioxidant and cytotoxic activities of Parijoto fruit extract and fractions on HeLa cell line. The fruits were extracted using ethanol 70% and fractionated by hexane and ethyl acetate. Furthermore, the fraction was analyzed for secondary phytochemical metabolite content using thin-layer chromatography and staining reagents. The antioxidant and cytotoxic activities were determined using the DPPH scavenging assay and the MTT assay, respectively. The ethanol extract and fraction contained flavonoid and tannin compounds. Ethanol extract, ethanol fraction, and ethyl acetate fraction of Parijoto fruit had an antioxidant activity with IC50 values of 77.3, 88.64, and 46.61 μg/mL, respectively. Ethyl acetate fraction showed the highest activity on HeLa cells with an IC50 value of 45.57 μg/mL compared to ethanol extract, ethanol fraction and n-hexane fraction with an IC50 value of 233.43, 700.75, and 534.30 μg/mL, respectively. Based on these results, the ethyl acetate fraction of Parijoto fruit had the potency to be explored further to elucidate their cytotoxicity mechanism in HeLa cells.Keywords: antioxidant activity, cytotoxicity, Medinilla speciosa, Parijoto fruit fractions.
Co-Authors Andy Eko Wibowo Ingenida Hadning Linta Sabila Hanik Lutfi Chabib, Lutfi Muhammad Thesa Ghozali Pinasti Utami, Pinasti Rifki Febriansah Rifki Febriansyah Yandi Syukri