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All Journal Jurnal Kesehatan Masyarakat Biosaintifika: Journal of Biology & Biology Education
Surniyantoro, Harry Nugroho Eko
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Agriculture, Biological Sciences & Forestry Public Health

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The Cytogenetic Effects on Peripheral Blood Lymphocytes in Cancer Patients After Radiation Therapy: Chromosome Aberrations and Micronuclei Lusiyanti, Yanti; Surniyantoro, Harry Nugroho Eko; Rahajeng, Nastiti; Suvifan, Viria Agesti; Purnami, Sofiati; Choridah, Lina
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.25264

Abstract

Individual responses to radiotherapy are often observed whether or not regimes with identical treatments were applied. Patient-related factors, the therapeutic process, and therefore the intrinsic factors of individual radiosensitivity are considered to be causing the variability of side effects. A preliminary evaluation was done on cytogenetic biomarkers found in cancer patients. The purpose of this present study was to assess the individual response of patients with cancers after radiation therapy. The sample obtained from 11 patients with different types of cancer as a case group and 12 people as a control group from a healthy volunteer. Blood samples were stimulated by an in vitro culture using phytohemagglutinin, and the cultures were assessed by using the Dicentric and Cytokinesis- Block Micronucleus (CBMN-) assay. These two methods were compared. The results showed that the overall dicentric chromosome and micronuclei in binucleate cells (MN/BNC) have a significantly higher frequency in the breast, head, and neck compared to extremity cancer. A high frequency of micronuclei in lymphocyte patients was seen after radiotherapy treatment but relatively not much higher compared to the range of micronuclei backgrounds in healthy people The CBMN is the most effective assay for evaluation of the cytogenetic studies in cancer patients because it is more radiosensitive to study individual responses. By evaluating the effects of radiotherapy based on DNA damage, the severity of radiation exposure can be studied. This study can be useful for researchers and related stakeholders in the application of radiotherapy.
Worker Health Monitoring Through Whole Body Counter Examination for Safety and Radiation Protection (2017-2018 Data) Surniyantoro, Harry Nugroho Eko; Sugiyana, Sugiyana; Prasetio, Heru; Ikram, Abarrul; Syaifudin, Mukh
KEMAS: Jurnal Kesehatan Masyarakat Vol 17, No 2 (2021): KEMAS (Article in Press)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v17i2.25911

Abstract

Abstract. Radiation protection is an action or effort taken to reduce the effects of damaging radiation exposure. Therefore, it is necessary to routinely monitor the condition of workers, among others, with a whole-body counter (WBC) either for daily activities or in a radiation emergency. In this research 78 male and female workers with an average age of 44.51 years who have been worked for 1 up to more than 30 years at the Center for Technology of Radiation Safety and Metrology (PTKMR) - BATAN in 2018-2019 have been determined with standard procedures. The results showed that the average K-40 count for the year 2018 was 3767.35 ± 975.33 Bq. Among the 80 participants tested, internal radioisotopic contamination with Cs-137 was detected in one person, but the levels were marginal and just exceeded the detection limit (250 Bq/body). This average value was lower than that of the previous year (2017) i.e. 4274.74 ± 740.85 Bq for 43 respondents. There is no positive correlation between radioactivity with the bodyweight of respondents but correlated positively with body mass index. It was concluded that the radioactivities of the workers of PTKMR are still within a normal range.
Worker Health Monitoring Through Whole Body Counter Examination for Safety and Radiation Protection (2017-2019 Data) Sugiyana, Sugiyana; Prasetio, Heru; Ikram, Abarrul; Syaifudin, Mukh; Surniyantoro, Harry Nugroho Eko
KEMAS: Jurnal Kesehatan Masyarakat Vol 17, No 2 (2021)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v17i2.25911

Abstract

Radiation protection is an action or effort taken to reduce the effects of damaging radiation exposure. Therefore, it is necessary to routinely monitor the condition of workers, among others, with a whole-body counter (WBC) either for daily activities or in a radiation emergency. In this research 78 male and female workers with an average age of 44.51 years old who have been worked for 1 up to more than 30 years at the Center for Technology of Radiation Safety and Metrology (PTKMR) - BATAN in 2018-2019 have been determined with standard procedures. The results showed that the average K-40 count for the year 2018 was 3767.35 ± 975.33 Bq. Among the 80 participants tested, internal radio-isotopic contamination with Cs-137 was detected in one person, but the levels were marginal and just exceeded the detection limit (250 Bq/body). This average value was lower than that of the previous year (2017) i.e. 4274.74 ± 740.85 Bq for 43 respondents. There is no positive correlation between radioactivity with the bodyweight of respondents but correlated positively with body mass index. It is concluded that the radio-activities of the workers of PTKMR are still within a normal range. 
Co-Authors Abarrul Ikram Heru Prasetio Lina Choridah Mukh Syaifudin Rahajeng, Nastiti SOFIATI PURNAMI Sugiyana Sugiyana, Sugiyana Viria Agesti Suvifan Yanti Lusiyanti