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All Journal BERITA BIOLOGI Biosaintifika: Journal of Biology & Biology Education Manilkara: Journal of Bioscience
Kartika, Titik
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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JAMUR ENTOMOPATOGEN DAN AKTIVITAS ENZIM EKSTRASELULERNYA Suciatmih, Suciatmih; Kartika, Titik; Yusuf, Sulaeman
BERITA BIOLOGI Vol 14, No 2 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i2.1818

Abstract

Isolation of entomopathogenic fungi and their extracellular enzyme activity from rhizosphere soil were carried out. Soil samples were collected from rhizosphere under Arecaceae plant collection and a shrub in Bogor Botanic Garden, West Java; and another samples were gathered from forest floor on peat land in Sebangau National Park, Central Kalimantan. Insect bait method was used to catch fungi from soil samples by using Coptotermes sp. termites and Xystrocera festiva larva in the laboratory. A total of 38 fungal isolates belonging to 18 species and 12 genera were successfully isolated and identified. Isolated fungi were dominated by Deuteromycotina; and Fusarium is the most common (5 species). Peat and Pinanga coronata rhizospheres; and termites bait gave the highest fungal diversity of 9 species respectively. All fungal isolates did not indicate chitinase activity, but 60.53 %, 10.53 % and 13.16 % had both of lipase and protease; lipase and protease activities, respectively. Only 15.79 % fungal isolates were negatively in lipase or protease activities.
Larvicidal Activity of Brugmansia candida against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae) Himmi, Setiawan Khoirul; Tarmadi, Didi; Meisyara, Dita; Fajar, Anugerah; Kartika, Titik; Guswenrivo, Ikhsan; Yusuf, Sulaeman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24634

Abstract

Mosquitoes are well known as vectors of hazardous diseases for human. Plant extracts can be used as an alternative for larval control due to they are a rich source of bioactive chemicals and safe for the environment. The present study investigated the larvicidal activity of crude extracts derived from leaf and flower of Brugmansia candida against the second larval instar of Aedes aegypti and Culex quinquefasciatus. The larval mortality was observed at 24 and 48 h exposure of both leaf and flower extracts, at the concentration of 100, 250, 500, and 1000 ppm. The 24 h exposure of both extracts at the concentration of 500 and 1000 ppm resulted in larval mortality rates were significantly lower than those of 48 h exposure. However, the mortality rate was not significantly different at the lower concentrations of crude extracts. The results also suggested that there was no significant difference in the larvicidal effect between leaf and flower extracts at 24 and 48 h exposure for all concentrations. The LC50 values at 48 h exposure for leaf extract were 789 and 791 ppm for Ae. aegypti and C. quinquefasciatus, respectively, whereas for flower extract were 772 and 780 ppm for Ae. aegypti and C. quinquefasciatus, respectively. Overall, B. candida showed larvicidal activity against Ae. aegypti and C. quinquefasciatus. This research contributes to new finding regarding the larvicidal activity of B. candida. This finding also supports the next study to develop B. candida as an alternative source for larval control agent.
Analisis Kemampuan Produksi Auksin dari Bakteri Endofit dan Plant Growth Promoting Rhizobacteria (PGPR) dalam Akar Cabai Merah Keriting (Capsicum annum L.) Maulida, Nurdalila; Rahmawati, Arrum; Dewi, Tirta Kumala; Simarmata, Rumella; Widowati, Tiwit; Kartika, Titik; Guswenrivo, Ikhsan; Nugroho, Deddy Triyono; Supriatna, Ateng
MANILKARA: Journal of Bioscience Vol 3 No 1 (2024): Agustus
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33830/manilkara.v3i1.9817.2024

Abstract

Endophytic bacteria found in the roots, stems, and leaves of plants have potential as biocontrol agents and drivers of plant development, besides that in plant roots there are also plant growth promoting rhizobacteria (PGPR) with the same ability. The consortium of the two bacteria will produce Indole 3 acetic acid (IAA) compounds which are then converted into auxins that are beneficial to plants. The research aims to determine the ability of endophytic bacteria and PGPR from the roots of curly red chili plants in producing auxin hormones. The research work procedure includes rejuvenation of bacterial isolates, macroscopic and microscopic characteristics, measuring auxin levels using HPLC (High Performance Liquid Chromatography). The results showed that the consortium of 6 bacteria (3 endophytes and 3 PGPR) produced an auxin concentration of 46.122 ppm, a consortium of endophytic bacteria of 24.201 ppm, a consortium of PGPR bacteria of 162.723 ppm, and finally single endophytic bacteria and single PGPR bacteria produced auxin of A 158.913 ppm; B 64.882 ppm; C 93.923 ppm; CB1 240.817 ppm; CB2 186.807 ppm; and CMBC 11.689 ppm, while for the control 0 ppm. In conclusion, the highest auxin concentration was obtained from the single result of PGPR bacteria CB1 240.817 ppm.
Co-Authors Ateng Supriatna Deni Zulfiana Dewi, Tirta Kumala Fajar, Anugerah Guswenrivo, Ikhsan Himmi, Setiawan Khoirul Ismayati, Maya Maulida, Nurdalila Meisyara, Dita Nugroho, Deddy Triyono Rahmawati, Arrum Rumella Simarmata, Rumella Suciatmih, Suciatmih Tarmadi, Didi Tiwit Widowati, Tiwit Yusuf, Sulaeman Zulfitri, Apriwi