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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences BERITA BIOLOGI Wahana Forestra: Jurnal Kehutanan Biosaintifika: Journal of Biology & Biology Education
Yusuf, Sulaeman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Biological Control of Wood Destroying Organism Using Plant Extracts Collected from Mt. Merapi National Park, Indonesia Ismayati, Maya; Zulfiana, Deni; Tarmadi, Didi; Lestari, Anis Sri; Krishanti, Ni Putu Ratna Ayu; Himmi, Setiawan Khoirul; Fajar, Anugerah; Yusuf, Sulaeman
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 3 (2019): Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i3.20102

Abstract

Due to the climate change and global warming, the biodiversity database has gained the attention of the government. In line with the Indonesian Biodiversity Strategy and Action Plan (IBSAP), we have collected plants with insecticidal activity based on the local wisdom. This program aimed to protect Indonesian biodiversity from deforestation along with the loss of the number of species. This research?s goal was to evaluate termiticidal and antifungal properties from some plant extracts collected from the Turgo forest area, Mt. Merapi National park, Java. Three potential plants were evaluated. Based on specimen identification, the three plant samples were Kina (Cinchona sp.), Kamadoh (Dendrocnide stimulans (L.f.) Chew), and Keremi (Homalanthus populneus (Geiseler) Pax). The phytochemical test showed that Kina contained alkoloid, falvonoid, saponin, and tannin, whereas Kamadoh and Keremi contained saponin and tanin, respectively. Overall, all plant extracts have the termiticidal activities and able to inhibit wood- decay fungi with the inhibition percentage around 60% - 100%. Leaf extracts of Kina (Cinchona sp) and Keremi (Homalanthus populneus (Geiseler) showed the highest activity as wood-decay fungi inhibitor. The disclosure of the potential of bioinsecticides from some plants originating from Mount Merapi is very important before being lost due to deforestation and the Mount Merapi disaster. Thus, the potential bioinsecticide in these plants can increase its economic value as a substitute for synthetic insecticides that are friendly to the environment.
JAMUR ENTOMOPATOGEN DAN AKTIVITAS ENZIM EKSTRASELULERNYA Suciatmih, Suciatmih; Kartika, Titik; Yusuf, Sulaeman
BERITA BIOLOGI Vol 14, No 2 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i2.1818

Abstract

Isolation of entomopathogenic fungi and their extracellular enzyme activity from rhizosphere soil were carried out. Soil samples were collected from rhizosphere under Arecaceae plant collection and a shrub in Bogor Botanic Garden, West Java; and another samples were gathered from forest floor on peat land in Sebangau National Park, Central Kalimantan. Insect bait method was used to catch fungi from soil samples by using Coptotermes sp. termites and Xystrocera festiva larva in the laboratory. A total of 38 fungal isolates belonging to 18 species and 12 genera were successfully isolated and identified. Isolated fungi were dominated by Deuteromycotina; and Fusarium is the most common (5 species). Peat and Pinanga coronata rhizospheres; and termites bait gave the highest fungal diversity of 9 species respectively. All fungal isolates did not indicate chitinase activity, but 60.53 %, 10.53 % and 13.16 % had both of lipase and protease; lipase and protease activities, respectively. Only 15.79 % fungal isolates were negatively in lipase or protease activities.
Antimicrobial Production by an Actinomycetes Isolated from The Termite Nest Krishanti, Ni Putu Ratna Ayu; Zulfina, Deni; Wikantyoso, Bramantyo; Zulfitri, Arpiwi; Yusuf, Sulaeman
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (497.087 KB)

Abstract

Actinomycetes are Gram positive bacteria that have been detected in diverse ecological niches. Their member species are known to be a main source of various bioactive compounds. The discovery of Actinomycetes from diverse and unexplored resources has also been linked to increased opportunities to obtain novel bioactive compounds. Insect nest material is being investigated as a new source of novel antimicrobial producing Actinomycetes, which could be harnessed for therapeutic potential. A total of 10 Actinomycetes isolates were collected from the nest of Nasutitermes sp. in Pananjung Pangandaran Nature Reserve. These isolates were evaluated for antimicrobial activity against the challenge bacteria (Eschericia coli, Staphylococcus aureus, Bacillus subtilis, Serratia Marcescens) and fungi (Fomitopsis palustris, Fusarium oxysporum, Trichoderma viridae) by dual culture method. The result revealed that several isolate were active against fungi and bacteria. Isolate Pn-TN2 showed the highest level of antibacterial inhibition and the highest antifungal inhibition with Inhibition Rate value more than 80%. By morphological and 16S rRNA gene sequence analysis strongly suggested that the isolate Pn-TN2 belonged to Streptomyces prasinopilosus. We suggested that termite nest is a potential source of bioactive strains of cultivable Actinomycetes for future biotechnological needs.
SIFAT FISIS KAYU BERKUALITAS RENDAH DARI RIAU SETELAH MELALUI PROSES PENGAWETAN POHON DENGAN TEKNIK BANDAGE DAN INFUS Sribudiani, Evi; Somadona, Sonia; Sulaeman, Rudianda; Syafrinal, Syafrinal; Yusuf, Sulaeman; Amin, Yusup; Tarmadi, Didi; Pramasari, Dwi Ajias; Damayanti, Ratih; Djarwanto, Djarwanto
Wahana Forestra: Jurnal Kehutanan Vol. 14 No. 2 (2019)
Publisher : Fakultas Kehutanan Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/forestra.v14i2.3515

Abstract

The potential of high-quality wood for various purposes over the past decade is very difficult to obtain. This is due to the high rate of doforestation and forest degradation, especially in Riau Province. Examples of the use of high-quality wood, especially in Riau Province, is as material for creating 'pathways' in the context of preserving track culture in the Kuantan Singingi Regency. The purpose of this study is to determine the physical properties of red balam wood and bintangur that grow in Riau before and after the preservation process, and determine the strength class of red balam wood and bintangur that grows in Riau. The research was carried out on the starur trees and red balam with a diameter of 34-38 cm, tree height 18-22 m. The tree sample comes from the Rumbio customary forest and the Minas Tahura KPHP Riau Province. Examination of physical properties using British standards in 1957. The results showed that based on specific gravity, the red balam wood and bintangur before preserved and included as strong class II. The average moisture content of each wood is 14-30%. The largest wood shrinkage development in each wood occurs at the base of the wood.
Larvicidal Activity of Brugmansia candida against Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae) Himmi, Setiawan Khoirul; Tarmadi, Didi; Meisyara, Dita; Fajar, Anugerah; Kartika, Titik; Guswenrivo, Ikhsan; Yusuf, Sulaeman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.24634

Abstract

Mosquitoes are well known as vectors of hazardous diseases for human. Plant extracts can be used as an alternative for larval control due to they are a rich source of bioactive chemicals and safe for the environment. The present study investigated the larvicidal activity of crude extracts derived from leaf and flower of Brugmansia candida against the second larval instar of Aedes aegypti and Culex quinquefasciatus. The larval mortality was observed at 24 and 48 h exposure of both leaf and flower extracts, at the concentration of 100, 250, 500, and 1000 ppm. The 24 h exposure of both extracts at the concentration of 500 and 1000 ppm resulted in larval mortality rates were significantly lower than those of 48 h exposure. However, the mortality rate was not significantly different at the lower concentrations of crude extracts. The results also suggested that there was no significant difference in the larvicidal effect between leaf and flower extracts at 24 and 48 h exposure for all concentrations. The LC50 values at 48 h exposure for leaf extract were 789 and 791 ppm for Ae. aegypti and C. quinquefasciatus, respectively, whereas for flower extract were 772 and 780 ppm for Ae. aegypti and C. quinquefasciatus, respectively. Overall, B. candida showed larvicidal activity against Ae. aegypti and C. quinquefasciatus. This research contributes to new finding regarding the larvicidal activity of B. candida. This finding also supports the next study to develop B. candida as an alternative source for larval control agent.
Co-Authors Deni Zulfiana Desyandri Desyandri Djarwanto Djarwanto Evi Sribudiani Fajar, Anugerah Guswenrivo, Ikhsan Himmi, Setiawan Khoirul Ismayati, Maya Kartika, Titik Krishanti, Ni Putu Ratna Ayu Lestari, Anis Sri Meisyara, Dita Pramasari, Dwi Ajias Rudianda Sulaeman Somadona, Sonia Suciatmih, Suciatmih Syafrinal, Syafrinal Tarmadi, Didi Wikantyoso, Bramantyo Yusup AMIN Zulfina, Deni Zulfitri, Arpiwi