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All Journal Jurnal Bahan Alam Terbarukan The Journal of Pure and Applied Chemistry Research Abdimas Galuh : Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Papua
Permatasari, Nur Umriani
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Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Other

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Journal : The Journal of Pure and Applied Chemistry Research

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Potential Cassava Skin Waste (Manihot esculenta C.) In the Production of Bioethanol by Enzymatic Hydrolysis and Fermentation Using Zymomonas mobilis Bacteria Permatasari, Nur Umriani; Dali, Seniwati; Wahyuni, Eka Sry
The Journal of Pure and Applied Chemistry Research Vol 12, No 2 (2023): May-August 2023
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2023.012.02.3304

Abstract

Cassava is one of the most widely produced agricultural products in Indonesia with cassava peel waste of 20%. Cassava peel contains carbohydrates and stores a high cellulose content so that it has a potential to be a bioethanol. This study aims to utilize cassava peel waste for bioethanol production with an alkaline pretreatment process, cellulase enzyme hydrolysis and fermentation by using Zymomonas mobilis bacteria. Alkaline pretreatment with 14% NaOH is used to hydrolyze lignocellulose. The hydrolysis optimization process enzymatically applies the Surface Response Method (RSM) to determine the optimum conditions at hydrolysis pH in the range of 2-10 and hydrolysis temperature in the range of 30-70 °C by analyzing glucose levels using the Dinitrosalicylic Acid (DNS) method and UV-vis spectrophotometry instruments. Surface Response Method (RSM) is likewise implemented to decide the greatest conditions of the fermentation process. The pH measurement ranges 2-10, and fermentation time takes 6 to 168 hours. Based on the results of research, it results a lignin content of 6.68% (b/b), cellulose content of 58.4% (b/b), and hemicellulose content of 27.3% (b/b). The optimum conditions of the hydrolysis process obtained an optimum glucose level of 9.22mg/mL at pH 2 and a hydrolysis temperature of 50°C. The optimum conditions of the fermentation process use Zymomonas mobilis at pH 6 while fermentation time takes 168 hours analyzed using a refractometer produced a bioethanol content of 37.75% (v/v) and a gas chromatography produced a bioethanol content of 54.94% (v/v).
Co-Authors Abd. Karim, Abd. Ananda, St. Namira Anggriani, Dhea Faraditha Dwayana, Zaraswati Gani, Fuad Hasnah Natsir Johanes, Eva Muhammad, Syafrian Nur Musa, Bulkis Nur Haedar Nur Haedar Ruslan Umar Seniwati Dali Situmorang, Sarah Victoria Octovianni Wahyuni, Eka Sry Zhafirah, Nur Afifah