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POTENSI ABALON TROPIS Haliotis asinina L. SEBAGAI SUMBER INOKULUM JAMUR SIMBION PENGHASIL ANTIMIKROBA Magdalena Litaay; Karlina Sari; Risco B Gobel; Nur Haedar
Jurnal Ilmu Kelautan SPERMONDE VOLUME 3 NOMOR 1, 2017
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v3i1.2124

Abstract

The research about “The Potencial of Tropical Abalone Haliotis asinina L. As Source of Mushroom Antimicroba ProducingSymbionts” had been done. This research aimed to know the abalone potency as a source of inoculum and to characterizeisolate fungal symbionts H. asinina L. Isolation of fungi symbionts H. asinina L. was performed used a PDA medium (PotatoDextrose Agar). Characterization of isolates fungal symbiont from H. asinina L. consists of macroscopic and microscopicobservations, and activity testing against pathogenic bacteria and fungi. The results showed that there were isolates of fungalsymbionts H. asinina L. (Abl.J.1, Abl.J.2, and Abl.J.3). The results of macroscopic observation colony indicated Abl.J.1 andAbl.J.3 isolate had a surface likes flour and Abl.J.2 isolate had a flat surface such as cotton; Abl.J.1 isolate green, Abl.J.2 isolatelight green and Abl.J.3 isolate black in colours. Three isolates had concentric circles; isolates Abl.J.1 and Abl.J.3 had radiallines and isolate Abl.J.2 had not radial line. The result of microscopic observation showed that three isolates had not septa,and hyaline (colorless); three isolates had asexual spores conidioshpore and all isolates was suspected to belong to the genusAspergillus. All isolates were able to inhibit the growth of Salmonella thypi bacteria and Candida albicans fungus and theresulting compounds were bacteriocidal and fungicidal.Keyword: Gastropods, H. asinina L., Symbiont fungus, Antimicrobal, Aspergillus.
Ekplorasi dan Karakterisasi Biokimia Bakteri Resisten Timbal (Pb) dari Sungai Tallo Makassar Fahruddin Fahruddin; Nur Haedar; Slamet Santosa; Sri Wahyuni
Jurnal Serambi Engineering Vol 5, No 3 (2020)
Publisher : Fakultas Teknik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32672/jse.v5i3.2133

Abstract

Industrial progress and waste of urban domestic activities have an impact on the Pb heavy metal pollution on the environment, this will have an impact on human health. Pb metal can be overcome with biological methods by utilizing bacteria in reducing Pb metal. The study aimed to obtain the species  of resistant bacterial isolates of Pb metal and determine its characteristics. Sediment and water samples were obtained from the Tallo River. Isolation and selection of Pb metal resistant bacteria were carried out on nutrient agar media is 10 ppm added of PbAgNO3, afterwards the colonies that grew and differed were characterized by morphological morphology and several biochemical tests. The characterization results obtained 8 isolates of Pb metal resistant bacteria consisted of 8 isolates from sediment samples and 3 isolates from water samples. The characteristics of each bacterial isolate on cell morphology are the same and biochemical tests show different results.
EDIBLE COATING BERBASIS PATI SINGKONG DENGAN PENAMBAHAN EKSTRAK JAHE MERAH SEBAGAI ANTIJAMUR UNTUK MEMPERPANJANG UMUR SIMPAN CABAI MERAH Capsicum annuum L Henra -; Eva Johannes; Nur Haedar
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 8 No. 2 (2023): Bioma : Juli - Desember 2023
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cabai merah Capsicum annuum L. merupakan komoditas yang rentan terhadap kerusakan dan mudah mengalami pembusukan oleh jamur karena kadar air yang tinggi sehingga tidak dapat bertahan lama. Penelitian ini bertujuan untuk mengetahui pengaruh edible coating dengan penambahan ekstrak jahe merah dalam menghambat pertumbuhan jamur pembusuk serta mengetahui umur simpan cabai merah. Jamur pada cabai merah diisolasi dengan menggunakan metode penanaman langsung (direct planting) dan diperoleh jamur Aspergillus flavus. Selanjutnya dilakukan uji daya hambat terhadap jamur Aspergillus flavus menggunakan ekstrak jahe merah konsentrasi 5%, 10%, 20% dan 40%. Hasil yang diperoleh menunjukkan ekstrak jahe merah mampu menghambat pertumbuhan jamur Aspergillus flavus serta konsentrasi yang tepat sebagai pengawet adalah konsentrasi 5%. Pengamatan hasil pengaplikasian edible coating dilakukan melalui uji susut bobot, uji tekstur, uji warna dan uji Total Plate Count (TPC). Hasil yang diperoleh menunjukkan pengaplikasian edible coating dengan penambahan ekstrak jahe merah dapat menurunkan susut bobot, memperlambat pelunakan dan mempertahankan warna dari cabai merah dengan jumlah koloni jamur yang relatif sedikit yaitu 2,8 x 102 CFU/mL dibandingkan perlakuan kontrol dengan jumlah koloni jamur 2,3 x 104 CFU/mL. Dari hasil tersebut memperlihatkan edible coating dengan penambahan ekstrak jahe merah dapat memperpanjang umur simpan sampai pada hari ke-9, serta mampu menghambat pertumbuhan jamur pembusuk pada cabai merah. Kata Kunci : Edible coating, Cabai merah, Ekstrak jahe merah, Aspergillus flavus