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All Journal Biospecies Biology, Medicine, & Natural Product Chemistry Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Jurnal Prima Medika Sains
Natali, Oliviti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Medicine & Pharmacology Nursing Public Health

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Isolasi Lactobacillus sp dari susu segar kemasan dan uji antimikroba terhadap bakteri patogen Salmonella sp dan Streptococcus mutan Lubis, Adhayani; Natali, Oliviti; Arhamni, Ade; Masyitah, Masyitah; Ariyanto, Ariyanto; Marpaung, Hans Hotma Haposan; Panjaitan, Linda
Jurnal Prima Medika Sains Vol. 3 No. 1 (2021): Juni
Publisher : Program Studi Magister Kesehatan Masyarakat Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/jpms.v3i1.1763

Abstract

Nilai gizi yang tinggi pada susu merupakan media yang baik untuk pertumbuhan berbagai macam mikroorganisme, baik yang menguntungkan maupun yang dapat membahayakan manusia. Penelitian ini bertujuan menganalisis kemampuan Lactobacillus sp yang diisolasi dari susu segar terhadap bakteri patogen Gram (+) dan Gram (-) yaitu Salmonella sp, Streptococcus mutan secara in vitro. Hasil penelitian menunjukkan total populasi BAL pada susu segar kemasan adalah 200x108 CFU/g. Pada pewarnaan Gram menujukkan hasil pewarnaan Gram yang positif, sedangkan pada uji katalase menjukkan reaksi yang negatif dan uji aktivitas antimikroba yang menggunakan metode difusica kram MRS broth. Susu segar kemasan mampu menghambat Salmonella sp dengan zona hambat tertinggi adalah 11,925 mm sedangkan pada Streptococcus mutans mampu menghambat dengan zona tertinggi yaitu 13,45 mm.
Uji efektifitas antibakteri ekstrak daun jambu biji (Psidium guajava) terhadap pertumbuhan bakteri Bacillus cereus Natali, Oliviti; Tarigan, Antje Irmella; Sarumpaet, Elpina; Salim, Susanto; Dewani, Yunita; Hanida, Wika; Yensuari, Yensuari
Jurnal Prima Medika Sains Vol. 3 No. 1 (2021): Juni
Publisher : Program Studi Magister Kesehatan Masyarakat Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/jpms.v3i1.1776

Abstract

Jambu biji (Psidium guajava L) secara medis banyak mengandung senyawa kimia yang berkhasiat (flavonoid, saponin, tanin, alkaloid dan minyak atsiri). Senyawa-senyawa tersebut diduga mampu menghambat pertumbuhan Bacillus cereus, yakni bakteri yang mengkontaminasi makanan serta menghasilkan racun penyebab diare. Tujuan penelitian ini untuk mendeskripsikan pengaruh ekstrak daun jambu biji terhadap pertumbuhan Bacillus cereus serta menentukan konsentrasi ekstrak daun jambu biji yang paling optimal dalam menghambat pertumbuhan bakteri Bacillus cereus. Penelitian ini menggunakan rancangan sistematis lengkap dengan 13 perlakuan dengan 3 pengulangan, yaitu pemberian kontrol negatif, kontrol positif, ekstrak murni, serta ekstrak daun jambu biji dengan pelarut 10%, 20%, 30%, 40%, 50%, 60%,70%, 80%, 90% dan 100%. Data yang diperoleh berupa diameter zona hambat, dianalisis menggunakan uji Kruskall Wallis. Hasil penelitian menunjukkan bahwa ekstrak daun jambu biji pada semua konsentrasi berpengaruh pada pertumbuhan Bacillus cereus dengan konsentrasi paling optimal yaitu sampai 100% yang menghasilkan diameter zona hambat sebesar 13 mm. Hasil penelitian juga menunjukkan bahwa semakin tinggi konsentrasi ekstrak daun jambu biji, maka daya hambatnya terhadap pertumbuhan Bacillus cereus juga semakin tinggi.
Liver Protection Effect of Red Rose Petal Methanol Extract in Copper-Induced Liver Injury in Male Wistar Rats Hutagalung, Caroline Febrina; Girsang, Ermi; Mutia, Maya Sari; Suhartina, Suhartina; Natali, Oliviti
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.507-513

Abstract

The human body requires low amounts of copper to maintain homeostasis, but high copper intake can cause various health problems. Many previous studies have been performed to look for natural products that can neutralize the excessive copper effect; one such natural product is a rose petal. Therefore, this study aims to investigate the liver protection effect of rose petals in copper-induced liver injury in male Wistar rats. This experimental study used thirty male Wistar rats that were grouped into five groups, including control, standard (quercetin), Rose Petal Methanol Extract-1 (400 mg/kg BW), 2 (800 mg/kg BW), and 3 (1,200 mg/kg BW). All groups were treated for 14 days, and all rats were given copper (II) sulfate suspension for the last three days. On the fifteenth day, all rats were sacrificed to obtain blood and liver tissue SGOT, SGPT, and liver histology study. The results showed that rose petal methanol extract significantly decreased SGOT and SGPT levels compared to the control group (P-value <0.05). The reduced area of ballooning degeneration and necrosis were in line with the increased dose of rose petal extract supported the liver protection effect of rose petal extract. Therefore, it can be concluded that rose petal methanol extract protects the liver against copper impacts.
- Uji Efektivitas Daun Kelor Terhadap Shigella Dysenteriae: Uji Efektivitas Daun Kelor Terhadap Shigella Dysenteriae Karo, Mellinia Alpatri Br; Ferdinanda, Fransiska; Natali, Oliviti; Nasution, Sri Wahyun
Biospecies Vol. 14 No. 1 (2021): January 2021
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v14i1.11222

Abstract

Diarrheal disease is a disease that often occurs in developing countries and one of the factors causing the incidence of diarrhea is the bacteria Shigella dysenteriae. Moringa oleifera Lamk leaves contain alkaloid compounds, tannins, saponins, flavonoids, triterpenes / steroids, and polyphenols which are reported to have high antimicrobial and antioxidant activity. This study aims to measure the effectiveness of Moringa oleifera Lamk leaf extract in inhibiting the growth of Shigella dysentriae. This study uses a Post-Test Only Control Group Design research design and uses the disc diffusion method where the extract on MHA media has been inoculated with Shigella dysentriae bacteria. The data taken is primary data, namely the inhibition zone formed at a concentration of 15%, 30%, 45 %, 60%, 75%. The test results have shown that Moringa oleifera Lamk leaves can inhibit bacteria at a concentration of 75% with an average inhibition zone of 91.1 mm.
Laporan Awal: Survei Berbasis Situs Daring Mengenai Kekambuhan Psoriaris Pascavaksinasi Coronavirus Disease (COVID)-19 di Sumatera Utara, Indonesia Chandra, Rudi; Oentari, Widyaningsih; Djohan, Djohan; Natali, Oliviti
Majalah Kedokteran Indonesia Vol 73 No 3 (2023): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.73.3-2023-972

Abstract

Introduction: Psoriasis is a chronic inflammatory skin disorder that is often associated with autoimmune conditions. Vaccination has been considered one of the external factors that can trigger psoriasis flare. Recently, there have been reports linking the COVID-19 vaccine to the occurrence of psoriasis flare. The objective of this study was to examine the impact of COVID-19 vaccination on the incidence of psoriasis flares in Indonesia. Methods: This web-based cross-sectional study utilized Google forms and was conducted from September 2020 to February 2021 in North Sumatera, Indonesia. The study included psoriasis patients aged less than 18 years who had received COVID-19 vaccines. Data were collected and analyzed descriptively. Result: Out of 130 respondents, most were women (60.8%) aged 26-35 years (33.1%) with a disease duration of 1-10 years (48.5%). The most common type of psoriasis before vaccination was plaque and scalp (20.4%). Approximately 80% had no history of COVID-19 infection prior to vaccination, and 71.5% received the Sinovac vaccine. Among them, 49 respondents (37.7%) experienced flares after vaccination, mainly following the first dose (57.1%) and predominantly with Sinovac (77.6%). Flares were more common in women (67.3%) aged 26-35 years (28.6%) with disease duration of 1-10 years (59.2%). Most flares occurred greater than 2 weeks after vaccination (51%), and the post-vaccination type of psoriasis was frequently plaque and scalp (24.5%). Conclusion: The incidence of psoriasis flares after COVID-19 vaccinations was 37.7%. The administration of COVID-19 vaccinations may play role as a potential trigger for flares in psoriasis patients.
Co-Authors Arhamni, Ade Ariyanto Ariyanto Dewani, Yunita Djohan Djohan Ferdinanda, Fransiska Girsang, Ermi Hutagalung, Caroline Febrina Karo, Mellinia Alpatri Br Lubis, Adhayani Marpaung, Hans Hotma Haposan Masyitah Masyitah Mutia, Maya Sari Nasution, Sri Wahyun Oentari, Widyaningsih Panjaitan, Linda Rudi Chandra Salim, Susanto Sarumpaet, Elpina Suhartina Suhartina, Suhartina Tarigan, Antje Irmella Wika Hanida, Wika Yensuari, Yensuari