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All Journal Bioma : Jurnal Biologi dan Pembelajaran Biologi Jurnal Riset Biologi dan Aplikasinya
Arifiyanto, Achmad
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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AKTIVITAS ENZIM HIDROLASE PADA PENAPISAN ISOLAT ACTINOMYCETES TOLERAN SALINITAS Sumardi, Sumardi; Qatrunada, Vanya; Farisi, Salman; Arifiyanto, Achmad; Ekowati, Christina Nugroho; Farisi, Salman
BIOMA Vol 6, No 1 (2021): BIOMA:JURNAL BIOLOGI DAN PEMBELAJARAN BIOLOGI
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/bioma.v6i1.3548

Abstract

Actinomycetesis one of the microorganisms which have the ability of enzymes hydrolases such as cellulose, amylase, protease, and manannase. Manufacturer of enzymes of the hydrolase can be used as a candidate probiotic. A candidate probiotic should have a good resistance in different environmental conditions including pH and salinity.  This research is conducted to filter the candidate’s probiotics from bacteria Actinomycetes that tolerance salinity and has the activity of the enzyme hydrolase. Testing the activity of the enzyme was preformed qualitatively on the variation of pH 4, 7, and 9.8.  Theability of Atinomycetesproduce enzymes hydrolases is indicated by the colony on media containing the substrate.From the test results, cellulase enzyme activity with the largest enzymatic index value is 3.5 AF2 isolates pH 9.8. Amylase enzyme activity with the largest enzymatic index value is 3.66 RH1 isolates pH 7. Protease enzyme activity with the largest enzyme index value is 0.66, AF2 isolates at pH 7. Mananase enzyme activity with the largest enzymatic index value is 0.85, namely AF2 isolates pH 4. In testing the effect of salinity test isolates were able to grow at variations in the concentration of NaCl 0, 3, and 6%.
Screening of Extracellular Enzymes on Serratia marcescens strain MBC1 Putri, Meishy Handerlin; Handayani, Kusuma; Setiawan, Wawan A; Damayanti, Berliana; Ratih, Cindy Lukyta; Arifiyanto, Achmad
Jurnal Riset Biologi dan Aplikasinya Vol 3, No 1 (2021)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v3n1.p23-29

Abstract

Hydrolase enzymes are a class of enzymes used to break down the organic substrates into simpler molecules. Hydrolase enzymes play a role in biocontrol, industry, and public health. One of the hydrolase enzyme producers is bacteria. Serratia marcescens strain MBC1 is a collection in the Microbiology Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, University of Lampung which enzymatic activities haven’t been known yet. This research aimed to determine the enzymatic activity of S. marcesescens strain MBC1 that can be used as a candidate for biocontrol agents, biosurfactant producers, industry, or in the health sector. The screening was carried out using a qualitative method plate assay using selective solid media for each test, with three replications for each test. The data obtained were analyzed statistically using analysis non-parametric kruskall-wallis test. The lipolytic activity of the S. marcescens strain MBC1 showed high efficacy with an enzymatic index of 5.52 and amylase, cellulases, protease, mannanase, and chitinase with enzymatic indexes, respectively, 4.17, 1.50, 0.69, 0.35, and 0.27. This enzyme activity of this isolate can be used as a candidate for biological control agents,biosurfactant producers, industry, or the health sector.
Co-Authors Christina Nugroho Ekowati, Christina Nugroho Damayanti, Berliana Farisi, Salman Kusuma Handayani, Kusuma Putri, Meishy Handerlin Qatrunada, Vanya Ratih, Cindy Lukyta Setiawan, Wawan A Sumardi .