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Produk Bioetanol Daging Buah Sawo (Manilkara zapota L.) secara Fermentasi Batch dengan Saccharomyces cerevisiae Eden, Yonatan; Rahayu, Sri Teguh
Archives Pharmacia Vol 1, No 2 (2019): ARCHIVES PHARMACIA
Publisher : Lembaga Penerbitan Universitas Esa Unggul

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ABSTRAKBuah sawo (Manilkara zapota L.) merupakan komoditas perkebunan yang sejauh ini kurang dimanfaatkan masyarakat Indonesia dan penelitian menunjukkan bahwa sawo memiliki kandungan karbohidrat yang sangat tinggi (72,8%). Pada penelitian ini senyawa karbohidrat yang terkandung pada daging buah sawo digunakansebagai substrat oleh Saccharomyces cerevisiae untuk membentuk senyawa alkohol. Adanya enzim intracellular β-glucosidase (GH1-1), mampu mengubah senyawa karbohidrat yang terkandung dalam daging buah sawo menjadi etanol. Pada penelitian ini etanol diperoleh dari hasil fermentasi selama 144 jam secara aaerob pada suhu ruang. Adanya variasi jumlah inoculum yang ditambahkan ke dalam medium fermentasi (5; 7,5 dan 10% (v/v) menunjukkan ada peningkatan kadar etanol yang dihasilkan, di mana berturut-turut 5,0; 6,0; dan 6,5% (v/v). Hasil pemurnian dengan metode destilasi pada suhu 78 ˚C juga memperlihatkan kemurnian produk yang tinggi, yakni berturut-turut 80, 82 dan 83%.  Oleh karena itu, produk biomassa ini dapat dikategorikan sebagai bahan alternatif penganti BBM.Kata kunci : Bioetanol, daging buah sawo, fermentasi batch, Saccharomyces cerevisiae. ABSTRACTSapodilla fruit (Manilkara zapota L.) is a commodity that’s very less useful in Indonesian and research shows that sapodilla fruit has high carbohydrate content (72.8%). In this study, the composition contained in sapodilla fruit was used as a substrate by Saccharomyces cerevisiae to convert carbohydrate to be alcohol compounds. The presence of intracellular enzymes, β-glucosidase (GH1-1) able to convert the carbohydrate compounds in sapodilla fruit into ethanol. In this study ethanol was obtained from fermentation for 144 hours by anaerob at room temperature. The variation in the amount of inoculum added to the fermentation medium (5; 7.5 and 10% (v / v) showed that there was an increase in the ethanol content produced (5.0; 6.0; and 6.5% (v / v)). The purification method by the distillation method at a temperature of 78 ˚C also assesses the high purity of the product (80, 82 and 83%).Keywords : Bioethanol, Manilkara zapota, batch fermentation, Saccharomyces Cerevisiae.
Antihypertensive and Antioxidant Activity of Herbal Medicine (Jamu) B on Rats Induced by 10% Fructose Eff, Aprilita Rina Yanti; Ayu Pertiwi, Reza; Ehe Hurit, Hermanus; Rahayu, Sri Teguh; Eden, Yonatan
Pharmaceutical Sciences and Research Vol. 9, No. 1
Publisher : UI Scholars Hub

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Abstract

Prolonged fructose intake can activate the renin-angiotensin-aldosterone system and enhance oxidative stress conditions, causing hypertension and hyperuricemia. This study aimed to determine the antihypertensive and antioxidant activity of Jamu B on the activity of superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) in the kidneys of Sprague Dawley rats induced by 10% fructose. Thirty rats were divided into six groups. Groups B1-B3 were Jamu groups at a dose of 0.12 g/kg BW (B1), 0.24 g/kg BW (B2), and 0.36 g/kg BW (B3); group C (positive control) was given captopril 0.1 g/kg BW, group D (negative control) was given only 10% fructose, and group E (normal control) was healthy rats. The 10% fructose was induced for eight weeks and the treatment was given at the week 9th and 10th. Jamu B reduced systolic and diastolic blood pressure at all doses, but not significantly different from the normal control group (p>0.05). The SOD activity in groups B1, B2, B3, and C was significantly different from the negative control group (pJamu B at dose of 0.12 g/kg BW; 0.24 g/kg BW; and 0.36 g/kg BW have antihypertensive and antioxidant activity in the kidneys of rats induced by 10% fructose. Nevertheless, Jamu B at a dose of 0.36 g/kg BW was the most effective as an antihypertensive and antioxidant.