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All Journal JURNAL ILMIAH PETERNAKAN TERPADU JURNAL KAJIAN VETERINER Jurnal Veteriner Nusantara
Nitbani, Heny
Laboratorium Anatomi, Fisiologi, Farmakologi Dan Biokimia, Fakultas Kedokteran Dan Kedokteran Hewan, Universitas Nusa Cendana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary Other

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Muscle Micromorphology and Histomorphometry of Sumba Ongole Cattle (Bos indicus) Chandraone P. Kefi Amtiran; Filphin A. Amalo; Inggrid T. Maha; Heny Nitbani
JURNAL ILMIAH PETERNAKAN TERPADU Vol 9, No 3 (2021)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v9i3.p265-278

Abstract

This study aims to knowing the micromorphology and histomorphometry of the sumba ongole cattle muscles. The sample used was 6 muscle samples consisted of the longissimus dorsi and bicep femoris muscles taken from three sumba ongole cattle slaughtered at the West Sumba slaughterhouse. Muscle tissue was fixed using 10% formalin and made histological preparations and hematoxylin-eosin (HE) staining. Histological preparations were observed using a light microscope with objective magnifications of 4, 10, 40, and 100 times. The results showed that the muscle micromorphology in the transverse section consisted of many fascicles formed by muscle fibers with cell nuclei at the edges, connective tissue, and intramuscular fat. Muscles in longitudinal sections are formed by muscle fibers with light dark stripes and connective tissue. The muscle fibers diameter, fascicle diameter and thickness of the connective tissue in the bicep femoris muscle is higher than the longissimus dorsi muscle. In contrast, the number of muscle fibers per fascicle is higher in the longissimus dorsi muscle. Muscle histomorphometry are strongly influenced by anatomical location and function of the muscles, as well as the presence of intramuscular fat.
Anatomical and Histological Morphology of The Esophagus and Proventriculus in Green Jungle Fowl (Gallus varius) from Alor Island Michaela Marisa Dael; Inggrid T. Maha; Filphin A. Amalo; Heny Nitbani
JURNAL ILMIAH PETERNAKAN TERPADU Vol 9, No 3 (2021)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v9i3.p291-310

Abstract

Green jungle fowl (Gallus varius) is a species of jungle fowl endemic Indonesia. This study aims to find out the anatomical and histomorphologIcal of esophagus and proventriculus of green jungle fowl from Alor island. Samples of esophagus and proventriculus were taken from 3 green jungle fowl collected in Alor Regency. Macroscopic study observations include size, location, shape, color and consistency, then sample is fixated in a 10% formalin and made histology preparations as well as haemotoxilin-eosin staining. The results showed that the cervical and thoracic esophagus length is 9.8 ± 0.43 cm and 5.5 ± 0.24 cm, and diameters are 0.55 ± 0.11 cm and 0.6 ± 0.22 cm. The green jungle fowl esophagus is pale red, soft constency,and has a smooth outer surface. Histologically, the walls of esophagus are consist of tunica mucosa, tunica submucosa, tunica muscularis ekterna, and tunica adventisia. Proventriculus green jungle fowl length is 3.23 ± 0.21 cm, diameter 1.83 ± 0.24 cm, and weight 2 ± 0 g. The proventriculus is fusiform, pale red in color, and has denser consistency than esophagus. Proventriculus walls are composed of tunica mucosa, tunica submucosa, tunica muscularis ekterna and tunica serosa.
KAJIAN HISTOKIMIA DISTRIBUSI KARBOHIDRAT PADA TESTIS SAPI SUMBA ONGOLE (Bos indicus) Filphin Adolfin Amalo; Inggrid Maha; Heny Nitbani; Cynthia Gaina
JURNAL KAJIAN VETERINER Vol 10 No 1 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i1.5740

Abstract

Testis is the male reproductive organ that performs both endocrine and exocrine functions. This study aimed to determine the distribution of carbohydrates in the testis of sumba ongole cattle. The sample consisted of six testis were collected from East Sumba Slaughter House, fixed in formalin 10 %, processed histologically, and continued with AB pH 2,5 - PAS staining. The result showed a weak alcian blue intensity (+) in the tunica albuginea and moderate intensity (++) in the interstitial connective tissue, basement membrane, and connective tissue in the lumen of the seminiferous tubules. A strong intensity (+++) to PAS staining was seen in spermatogonia, spermatocytes, and myoid cells. Spermatids, spermatozoa, and sertoli cells showed a negative reaction (-). In the intertubular area, Leydig cells and fibroblasts showed a strong PAS reaction (+++), connective tissue showed a moderate reaction (++). The intensity of the weak reaction (+) is indicated by the basement membrane of the seminiferous tubules. The variation in the distribution of acidic and neutral carbohydrates in the testis of sumba ongole cattle is correlated with the proliferation of different cell types and steroidogenic activity at various stages of testicular development.
Identifikasi Senyawa Bioaktif Ekstrak Daun Semak Bunga Putih (Chromolaena odorata) Asal Kota Kupang, Nusa Tenggara Timur Meity Marviana Laut; Nemay Anggadewi Ndaong; Filphin Adolfin Amalo; Inggrid Trinidad Maha; Heny Nitbani; Dede Rival Novian
JURNAL KAJIAN VETERINER Vol 10 No 2 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i2.8693

Abstract

Chromolaena odorata, a member of Asteraceae, is a tropical shrub that grows throughout the year and can dominate pastures. The tropical weed is known as a medicinal plant and has the potential as a natural insecticide. For local people in NTT, the crude extract of the leaves is used to treat myasis on the livestock. C. odorata is also used as silage or food alternative for livestock. This study aims to determine the content of bioactive compounds in C. odorata leaves and determine the extract yield value, as scientific validation of local wisdom in NTT. This research began with the collection of fresh C. odorata leaves, extraction by maceration using 70% ethanol as solvent, solvent evaporation, and phytochemical screening. Phytochemical screening showed that the leaf extract of C. odorata from Kupang City contained high amounts of flavonoids, tannins, alkaloids, triterpenes and steroids and were tested negative for saponins. The extract yield value is 6.09%, means that the extract contains bioactive compunds in high concentration. Further research needs to be done to find out in more detail the content of secondary metabolites from C. odorata leaves and its activity in promote wound healing on livestock in NTT.
Histomorfologi Dan Histomorfometri Otot Ayam Hutan Hijau (Gallus Varius) Asal Pulau Alor Meica Agatha Leli Paschalya Bengkiuk; Filphin A Amalo; Inggrid T Maha; Heny Nitbani
Jurnal Veteriner Nusantara Vol 5 No 2 (2022): Agustus 2022
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v5i2.5227

Abstract

Green jungle fowl is one of the endemic animals in Indonesia. One of the distribution areas of green jungle fowl in East Nusa Tenggara is the island of Alor. Until now there has been no research conducted to determine the histological structure of the green jungle fowl so that this study was conducted with the aim of knowing the histological structure and muscle histomorphometry of the green jungle fowl. The research samples were pectoralis and bicep femoris muscles taken from three green jungle fowl in Kalabahi, Alor Regency. Muscle tissue was fixed using 10% formalin, histological preparations were made and stained with hematoxylin and eosin (HE). The results showed that the muscle histomorphology in the transverse section showed that the skeletal muscle of the green jungle fowl was composed of muscle fibers in a polygonal shape with many nuclei at the edges and connective tissue. No intramuscular fat cells were found. In the longitudinal section, it consists of muscle fibers with a light dark line pattern, the cell nucleus is elongated at the edges and connective tissue. The results of muscle histomorphometry, namely the diameter of the muscle fibers, the diameter of the fasciculus and the thickness of the connective tissue in the bicep femoris muscle area were higher than the pectoralis muscle area. The number of muscle fibers in each fasciculus in the bicep pectoralis muscle area is more than in the bicep femoris muscle area. Muscle histomorphometry is influenced by species, breed/race, age, diet, activity level and anatomical location.
Gambaran Histologi Dan Histomorfometri Otot Babi Timor (Sus Scrofa Domesticus) Caroline Scolastika H Penga; Filphin A Amalo; Heny Nitbani; Inggrid T Maha
Jurnal Veteriner Nusantara Vol 5 No 1 (2022): Februari 2022
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn/vol5iss1pp122-132

Abstract

Babi timor (Sus scrofa domesticus) merupakan ternak monogastrik yang dapat memenuhi sumber protein hewani dan berpotensi untuk dikembangkan. Babi memiliki kemampuan dalam mengkonversi pakan yang kemudian diubah menjadi daging dan lemak dengan sangat efisien. Penentuan kualitas daging dapat ditentukan dengan mengamati struktur histologi dan mengukur parameter histomorfometri otot. Penelitian ini dilakukan dengan tujuan untuk mengetahui struktur histologi dan histomorfometri otot babi timor. Sampel penelitian berupa otot longissimus dorsi dan bicep femoris diperoleh dari tiga ekor babi timor di Pasar Ternak Lili-Camplong. Jaringan otot difiksasi dalam larutan formalin 10% dan dilakukan pembuatan sediaan histologi serta pewarnaan hematoksilin dan eosin (HE). Hasil penelitian menunjukkan mikromorfologi jarigan otot babi timor tersusun atas serabut-serabut otot berupa sel memanjang ditunjang oleh jaringan ikat, tersusun dalam berkas pararel dengan arah kontraksi terlihat jelas pada potongan longitudinal sedangkan pada potongan transversal serabut otot berbentuk poligonal dengan inti sel dibagian tepi membentuk banyak fasikulus, terdapat jaringan ikat (endomisium, perimisium, dan epimisium) serta deposisi jaringan lemak. Parameter histomorfometri jaringan otot babi timor yaitu diameter serabut otot, jumlah serabut otot per fasikulus, diameter fasikulus dan ketebalan jaringan ikat pada area otot longissimus dorsi lebih rendah dibandingkan pada area otot biceps femoris. Histomorfometri otot dipengaruhi oleh lokasi dan tingkat aktivitas otot, bangsa, umur, serta pakan/nutrisi.
Pengaruh Bentuk Pakan (Crumble Dan Pellet) Terhadap Pertumbuhan, Berat Karkas Dan Profil Saluran Pencernaan Ayam Broiler Rosalinda Kristanti Intan Tahu; Frans U Datta; Heny Nitbani
Jurnal Veteriner Nusantara Vol 5 No 1 (2022): Februari 2022
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn/vol5iss1pp143-153

Abstract

This research is an experimental type of research that uses the RAL method (Completely Randomized Design) with two treatments, namely complete feed in the form of granules (crumbles) and complete feed in the form of pellets (pellets). Each treatment used 35 broiler chickens aged 2 weeks. This study aimed to examine the effect of feed form (crumble and pellet) on growth, carcass weight and digestive tract profile of broiler chickens in the grower to finisher period. From the first day until the age of 12 days (starter period) chicks (DOC) were given complete feed in the form of crumbs. The research data were analyzed by Independent T test using SPSS. The results of data analysis showed that there was a significant effect of treatment (P<0.05) on proventriculus weight and no significant effect (P>0.05) on body weight, carcass weight, ventriculus weight, and weight and length of the small intestine of broiler chickens. It can be concluded that statistically, in the grower period, feed particle size had little effect on growth and carcass weight and digestive tract profile. However, empirically, the carcass weight of the granular group (807 g) versus the pellet group (858 g) was quite different so that economically, giving complete feed in the form of pellets was more profitable.
Gambaran dan Anatomi Ginjal Sapi Sumba Ongole (Bos indicus) Claudia Beatrice; Filphin A Amalo; Inggrid T Maha; Heny Nitbani
Jurnal Veteriner Nusantara Vol 6 No 1 (2023): Februari 2023
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v6i1.8560

Abstract

Sumba Ongole Cattle (Bos indicus) is one of the local breed cattle in Indonesia and is properly developing on the island of Sumba. One of the organ systems that has important role in cleanig the rest of metabolism in animals are kidney. This research is aimed to observe the structure of anatomy and histologycal of the kidney. This study uses 3 pairs of kidney of the male and female sumba ongole cattle with age range 3-5 years. Samples are colected in Rumah Potong Hewan (RPH) Regency of East Sumba. Macroscopic observations were performed, and sample were fixed in 10% formalin solution. Furthermore, on the histological preparations and continued with HE staining. The macroscopic of the kidney showed that sumba ongole cattle had lobed shape, elastic consistency, adn brownish red in color. Meanwhile in microscopic observations showed, there are glomerulus, Bowman’s capsule, urin space/ Bowman’s space, mesangial cell, macula densa, proximal convolated tubule, distal convulated tubule, and collecting tubule.
Uji Performa Pertumbuhan Dan Profil Sel Darah Merah Ayam Broiler (Fase Starter) Yang Diberi Pakan Tepung Magot Bsf (Black Soldier Fly) Sebagai Pakan Tambahan Pada Ransum Komersial Brechmans Nicolans Trison Ko'a; Frans U Datta; Heny Nitbani
Jurnal Veteriner Nusantara Vol 5 No 2 (2022): Agustus 2022
Publisher : Program Studi Kedokteran Hewan, Universitas Nusa Cendana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jvn.v5i2.8611

Abstract

This study aims to determine the effect of adding Black Soldier Fly (BSF) maggot flour at several levels (dose) in complete feed on feed consumption, ration conversion, growth (body weight gain) and red blood cell profile in broiler chickens. The research material used 100 chickens. This study used a Completely Randomized Design (CRD) with 4 treatments where each treatment was filled with 25 animals/treatment. Parameters observed were ration consumption, body weight gain, feed conversion and broiler blood cell profile. The data obtained will be presented in the form of a table and described descriptively. The results showed that the addition of Black Soldier Fly (BSF) maggot flour had an effect on body weight gain but did not show a significant difference between the treatment groups. The addition of BSF maggot flour did not affect the red blood cell profile where the total erythrocyte, hemoglobin, hematocrit and erythrocyte index showed normal values. The conclusion in this study was that the addition of maggot flour to commercial rations affected the degree of feed palatability, thus affecting the level of consumption and erythrocyte profile at the beginning of rearing and the addition of protein in the finisher phase had no effect on feed efficiency.
Co-Authors Brechmans Nicolans Trison Ko'a Caroline Scolastika H Penga Chandraone P. Kefi Amtiran Claudia Beatrice Filphin Adolfin Amalo Frans U Datta Gaina, Cynthia Dewi Inggrid Trinidad Maha Maha, Inggrid Trinidad Meica Agatha Leli Paschalya Bengkiuk Meity Marviana Laut Michaela Marisa Dael Nemay Anggadewi Ndaong Novian, Dede Rival Rosalinda Kristanti Intan Tahu