Sarjiya Antonius
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Cibinong

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Pengaruh Aplikasi Bacillus sp. 140-B dan Streptomyces sp. L.3.1-DW Terhadap Infeksi Fusarium oxysporum Schlecht f. sp. cubense (FOC) dan Pertumbuhan Tanaman Pisang (Musa acuminata) var. Cavendish Laili, Nur; Antonius, Sarjiya; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3660.313 KB) | DOI: 10.14203/jbi.v11i1.2157

Abstract

Fusarium oxysporum Schlecht f. sp. cubense (Foc) is the causal pathogen of wilt disease of banana. Abilities of  biocontrol agents Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW to control Foc infection in banana were studied. Application of Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as single isolate or their combination in banana were tested under greenhouse conditions for 30 days. The aims of this study were to evaluate the potential of  Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as biocontrol agents in banana. Treatments of biocontrol showed significant effect on the reduction of foc infection diseases of banana, compared to control. The lowest disease severity was found on the treatment of single isolate Streptomyces sp.L.3.1-DW with infection degree of 29,33%. Streptomyces sp. L.3.1-DW could suppress Foc population (6,25 x 105 CFU/ml) in rhizosphere area after 30 days innoculation. Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW were also act as plant growth-promoting rhizobacteria (PGPR), that indicated by improvement of banana growth, in which Streptomyces L.3.1-DW caused the highest growth of banana either with or without Foc infection.  This study indicated that Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW have potential as alternative solutions to control Fusarium wilt in banana var. Cavendish. Keywords: Bacillus sp. 140-B, banana, biocontrol, Foc, PGPR, Streptomyces sp. L.3.1-DW. 
Pengaruh Pemberian Kompos dan Pupuk Hayati terhadap Aktivitas Fosfatase dan Urease pada Tanah yang Ditanami Brokoli (Brassica oleracea L.) Rahmansyah, Maman; Antonius, Sarjiya
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2163

Abstract

Organic farming basically work on improving organic soil substrate which give impact to plant growth promoting bacteria. On the other hand, the use of agrochemicals leave chemical residues in the soil. In an effort to get the quality of organic matter and selected microbes as a biofertilizer material, this research had been done. Half ripe composting (HRC) process of organic substrate was utilized to enrich soil containing agrochemical residue, while ripe compost (RC) substance amended to soil free residue. That compost was used in broccoli cultivation. Microbial degrading pesticide was added to compost as biofertilizer formula. Even though plant biomass and the yield of flower was unclearly affected, but moreover the HRC application as due to “semi-on-site-composting” practices in soil containing agrochemical residue would cut off compost processing time. Soil microbial activity measured through soil respiration, urease and phosphomonoesterase indicated that the value was lower in samples collected from the soil free residue compared with soils containing residues. Utilization of chicken manure resulted in the availability of high nitrogen compounds and causing imbalance absorption by plants, compared with the use of HRC and RC in this study. Keywords: soil respiration, phosphatase, urease, Brassica oleracea L. 
PEMANFAATAN INOKULAN MIKROBA SEBAGAI PENGKAYA KOMPOS PADA BUDIDAYA SAYURAN [Microbial inoculants for compost enrichment on vegetables cultivation] Antonius, Sarjiya; Rahmansyah, Maman; Muslichah, Dwi Agustiyani
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (788.864 KB) | DOI: 10.14203/beritabiologi.v14i3.1824

Abstract

 Microbial existence is due to mineralize organic compound in soil and caused nutrients availability to the plants. In this study, some of microbial inoculants consist of a). Phosphate soluble bacteria (Pseudomonas sp.) collected from soils that intensively utilized agrochemical in its agriculture activities; b). Nitrogen - fixing bacteria Rhizobium leguminosarum; and c) Trichoderma sp. and Aspergillus sp.as organic degradation fungi were used for compost enrichment.These inoculants formulation were utilized to carrots (Daucus carota L.), broccoli (Brassica oleracea L.) and maize (Zea mays L.) cultivation in upland area, Cisarua, Bogor, West Java. This study was aimed to assess inoculants response to enrich fully mature and half ripe compost. Crops response to the inoculation treatment was varied among these commodities. In general, the response of plants with the use of compost enriched biofertilizer was positive, with an increase in yield of about 15- 30% for carrots, broccoli around 65- 90% and about 10% of corn crops. The significant increase in crops production as impact of organic fertilizier application indicated that C organic content of the organic farm of Cimelati and Agato were defisit.
Uji Potensi Bakteri Penghasil Hormon IAA (Indole Acetic Acid) dari Tanah Rhizosfer Cengkeh (Syzigium aromaticum L.) Sukmadewi, Desak Ketut Tristiana; Suharjono, Suharjono; Antonius, Sarjiya
Biotropika: Journal of Tropical Biology Vol 3, No 2 (2015)
Publisher : Biotropika: Journal of Tropical Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.932 KB)

Abstract

ABSTRAK Tanaman cengkeh (Syzygium aromaticum L.) merupakan salah satu komoditi perkebunan yang memiliki peranan penting bagi pendapatan devisa negara. Perkebunan cengkeh rutin mengunakan pupuk kimia sintetik dalam proses pertumbuhannya. Permintaan produk pertanian dan perkebunan yang bebas akan bahan kimia semakin meningkat. Tujuan dari penelitian ini adalah mempelajari potensi isolat  bakteri penghasil hormon IAA dari tanah rhizosfer tanaman cengkeh. Tahapan penelitian meliputi pengambilan sampel tanah, isolasi bakteri penghasil hormon IAA dan analisa hormon IAA. Bakteri yang berpotensi menghasilkan hormon IAA tertinggi adalah isolat TCKI 5 (32,84 ppm) dari Karangasem. Hasil tertinggi ini didapatkan pada waktu inkubasi 48 jam. Berdasarkan nilai OD terlihat bahwa isolat TCKI 5  pada waktu inkubasi  48 dan 72 jam memiliki nilai OD yang  sama yang  merupakan  fase stasioner   Kata kunci: bakteri, tanaman cengkeh, Indole Acetic Acid (IAA), rhizosfer 
Merah dan Pengaruhnya terhadap Biokimia Tanah Pada Percobaan Pot Menggunakan Tanah Ultisol Antonius, Sarjiya; Sahputra, Rozy Dwi; Nuraini, Yulia; Dewi, Tirta Kumala
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1258.925 KB) | DOI: 10.14203/jbi.v14i2.3744

Abstract

ABSTRACTUltisol dominated about 25% of the total land area in Indonesia. It has the potential to be used as agricultural land and shallot plants have a considerable prospect to be cultivated in ultisol soil.  Ultisol generally has not been handled properly. It needs some specific treatments due to the low nutrient contents, caused by the intensive leaching process. One of the effort to overcome the soil quality problems that occur in ultisol soils (high soil acidity, average pH <4.50, high Al saturation, and low macronutrient content such as P, K, Ca, Mg, and organic material content) is treated by the using the soil conditioner such as the supplying of organic materials in the form of compost and biochar. In this work, the biological organic fertilizer was also applied to increase microorganism activity in the soil. The experimental design used in this work was completely random design which has 8 combinations of soil treatment, compost, biochar and bio-organic fertilizer. Soil parameters measured were pH, C-Organic, P-Available, total bacterial population, soil respiration, and phosphomonoesterase enzyme activity. The agronomy parameters of shallot were also measured. The results showed that treatment of compost, biological organic fertilizer, and biochar have significant effect to increase soil microorganism activity in the form of total population of bacteria, soil respiration, P-Available and pH. The treatment also had a significant effect on supporting plant height at 2 and 6 MST, number of leaves at 2 MST, wet weight and dry weight of tubers. Keywords: biochar, bio-organic fertilizer, soil microorganism activity, shallot, ultisol 
FISIOLOGI PERTUMBUHAN, POTENSI AKTIFITAS PRODUKSI N2O DAN GEN FUNGSIONAL PENYANDINYA PADA BEBERAPA ISOLAT BAKTERI DENITRIFIKASI Agustiyani, Dwi; Laili, Nur; Antonius, Sarjiya
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4963.084 KB) | DOI: 10.14203/beritabiologi.v17i1.2340

Abstract

Physiological characters of four denitrifying bacteria (Bacillus sp. CPNS, Bacillus thuringiensis UPT1, Brevundimonas diminuta EA1 and Bacillus sp. UPSB) were studied based on the growth ability on various nitrate concentrations and the production of N2O gas. The characters of denitrifying bacteria were also evaluated through the existence of functional genes nirS and nosZ, encoding the nitrite reduction and nitrous oxide reduction enzymes which have important role on denitrification processes. The study showed that Bacillus sp. UPSB and Bacillus sp. CPNS isolates have a linear growth with the increasing concentration of KNO3. The N2O gas production of Bacillus sp. UPSB isolate was relatively high, about 70 ?/l, Bacillus sp. CPNS isolate was 25?/l, while the Bacillus thuringiensis UPT1 isolate was 5 ?/l and Brevundimonas diminuta EA1 isolate was 8 ?/l. It was also indicated that both Bacillus sp. UPSB and Bacillus sp. CPNS had high deninitrification activities. It was confirmed that all isolates were contained functional gen of nirS and nosZ.
PCR-based Specific Detection of Bacillus in Liquid Organic Fertilizer Hidayat, Iman; Laili, Nur; Agustiyani, Dwi; Antonius, Sarjiya
Journal of Microbial Systematics and Biotechnology Vol 1, No 1 (2019): June 2019
Publisher : Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (159.783 KB) | DOI: 10.37604/jmsb.v1i1.21

Abstract

Rapid molecular PCR-based detection method for Bacillus species used in the production of Beyonic® liquid organic fertilizer was carried out based on nucleotide sequence data from the 16S rRNA gene. The method involved sequencing the 16S rRNA gene of several Bacillus species and identifying around 16-22 specific nucleotide bases from 5' and 3' ends in the Bacillus 16S rRNA gene sequences. One specific primer pair for Bacillus detection was determined as follow: 5' - CAT AAG ACT GGG ATA ACT CCG GG - 3' (forward) from positions of 85-107 bp, and 5’ - CCA GGC GGA GTG CTT AAT GC - 3’ (reverse) from positions of 836-854 bp. PCR assay and gel electrophoresis analysis showed that the primer pair was specific to the genus Bacillus.
MERAH DAN PENGARUHNYA TERHADAP BIOKIMIA TANAH PADA PERCOBAAN POT MENGGUNAKAN TANAH ULTISOL Antonius, Sarjiya; Sahputra, Rozy Dwi; Nuraini, Yulia; Dewi, Tirta Kumala
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3744

Abstract

ABSTRACTUltisol dominated about 25% of the total land area in Indonesia. It has the potential to be used as agricultural land and shallot plants have a considerable prospect to be cultivated in ultisol soil.  Ultisol generally has not been handled properly. It needs some specific treatments due to the low nutrient contents, caused by the intensive leaching process. One of the effort to overcome the soil quality problems that occur in ultisol soils (high soil acidity, average pH <4.50, high Al saturation, and low macronutrient content such as P, K, Ca, Mg, and organic material content) is treated by the using the soil conditioner such as the supplying of organic materials in the form of compost and biochar. In this work, the biological organic fertilizer was also applied to increase microorganism activity in the soil. The experimental design used in this work was completely random design which has 8 combinations of soil treatment, compost, biochar and bio-organic fertilizer. Soil parameters measured were pH, C-Organic, P-Available, total bacterial population, soil respiration, and phosphomonoesterase enzyme activity. The agronomy parameters of shallot were also measured. The results showed that treatment of compost, biological organic fertilizer, and biochar have significant effect to increase soil microorganism activity in the form of total population of bacteria, soil respiration, P-Available and pH. The treatment also had a significant effect on supporting plant height at 2 and 6 MST, number of leaves at 2 MST, wet weight and dry weight of tubers. Keywords: biochar, bio-organic fertilizer, soil microorganism activity, shallot, ultisol 
PENGARUH APLIKASI BACILLUS SP. 140-B DAN STREPTOMYCES SP. L.3.1-DW TERHADAP INFEKSI FUSARIUM OXYSPORUM SCHLECHT F. SP. CUBENSE (FOC) DAN PERTUMBUHAN TANAMAN PISANG (MUSA ACUMINATA) VAR. CAVENDISH Laili, Nur; Antonius, Sarjiya; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2157

Abstract

Fusarium oxysporum Schlecht f. sp. cubense (Foc) is the causal pathogen of wilt disease of banana. Abilities of  biocontrol agents Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW to control Foc infection in banana were studied. Application of Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as single isolate or their combination in banana were tested under greenhouse conditions for 30 days. The aims of this study were to evaluate the potential of  Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as biocontrol agents in banana. Treatments of biocontrol showed significant effect on the reduction of foc infection diseases of banana, compared to control. The lowest disease severity was found on the treatment of single isolate Streptomyces sp.L.3.1-DW with infection degree of 29,33%. Streptomyces sp. L.3.1-DW could suppress Foc population (6,25 x 105 CFU/ml) in rhizosphere area after 30 days innoculation. Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW were also act as plant growth-promoting rhizobacteria (PGPR), that indicated by improvement of banana growth, in which Streptomyces L.3.1-DW caused the highest growth of banana either with or without Foc infection.  This study indicated that Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW have potential as alternative solutions to control Fusarium wilt in banana var. Cavendish. Keywords: Bacillus sp. 140-B, banana, biocontrol, Foc, PGPR, Streptomyces sp. L.3.1-DW. 
PENGARUH PEMBERIAN KOMPOS DAN PUPUK HAYATI TERHADAP AKTIVITAS FOSFATASE DAN UREASE PADA TANAH YANG DITANAMI BROKOLI (BRASSICA OLERACEA L.) Rahmansyah, Maman; Antonius, Sarjiya
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2163

Abstract

Organic farming basically work on improving organic soil substrate which give impact to plant growth promoting bacteria. On the other hand, the use of agrochemicals leave chemical residues in the soil. In an effort to get the quality of organic matter and selected microbes as a biofertilizer material, this research had been done. Half ripe composting (HRC) process of organic substrate was utilized to enrich soil containing agrochemical residue, while ripe compost (RC) substance amended to soil free residue. That compost was used in broccoli cultivation. Microbial degrading pesticide was added to compost as biofertilizer formula. Even though plant biomass and the yield of flower was unclearly affected, but moreover the HRC application as due to â??semi-on-site-compostingâ? practices in soil containing agrochemical residue would cut off compost processing time. Soil microbial activity measured through soil respiration, urease and phosphomonoesterase indicated that the value was lower in samples collected from the soil free residue compared with soils containing residues. Utilization of chicken manure resulted in the availability of high nitrogen compounds and causing imbalance absorption by plants, compared with the use of HRC and RC in this study. Keywords: soil respiration, phosphatase, urease, Brassica oleracea L.