Andi Salamah
Department of Biology, Faculty of Mathematics and Natural Sciences, University of Indonesia, Depok 16424

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Application of N2-Fixing Cyanobacteria Nostoc sp. SO-A31 to Hydroponically Grown Water Spinach (Ipomoea aquatic L.) Salamah, Andi; Fadilah, Nurrahmi; Khoiriyah, Istatik; Hendrayanti, Dian
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1867

Abstract

For this research, an application of cyanobacteria Nostoc sp. SO-A31 as a nitrogen source for the growth of water spinach (Ipomoea aquatica L.) was carried out using a modified Deep-Water Culture (DWC) hydroponic system, outdoors. A Hoagland medium was used for the growth medium, with the absence or presence of ammonium and nitrate as the nitrogen sources. A 0.7 g fresh weight biomass of 21-day-old Nostoc sp. SO-A31 was inoculated into the system. The four treatment media for this study were HA0 (Hoagland, ammonium free+inoculant), HN0 (Hoagland, nitrate free+inoculant), HA0N0 (Hoagland, ammonium free and nitrate free+inoculant), and HI (Hoagland with ammonium and nitrate +inoculant). AB-mix and complete Hoagland media were used as controls. The result showed that water spinach cultured on HA0 had good vegetative growth, as shown by the high yield of biomass, high number of leaves, high stem growth, and long roots. Inoculation of Nostoc sp. SO-A31 elongated the root of the water spinach plants in all treatments. The presence of Nostoc sp. SO-A31 in the complete Hoagland medium, though, caused chlorosis of the water spinach leaves. This study suggests that water spinach is a nitrate-dependent leafy vegetable.
INDEKS MITOSIS PUCUK DAUN Hibiscus rosa-sinensis L. VARIASI SINGLE PINK PADA BEBERAPA VARIASI WAKTU Iriani, Nur Annisa; Dwiranti, Astari; Salamah, Andi
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2099.219 KB) | DOI: 10.15408/kauniyah.v13i1.9454

Abstract

AbstrakHibiscus rosa-sinensis L. atau kembang sepatu merupakan tanaman hias yang memiliki banyak manfaat dan merepresentasikan sifat poliploidi. Tujuan penelitian ini yaitu untuk mengetahui Indeks Mitosis (IM) dan jumlah kromosom pucuk daun H. rosa-sinensis pada beberapa variasi waktu. Indeks Mitosis dan waktu pengambilan pucuk sangat diperlukan untuk studi kromosom karena pada tahap tersebut karakter-karakter kromosom dapat diamati dengan jelas dan mudah dihitung. Waktu pengambilan pucuk yang dilakukan yaitu pada 08:00, 10:00, 12:00, 14:00, 16:00 WIB. Pembuatan sediaan kromosom dilakukan menggunakan metode squash menggunakan pewarna Aceto-orcein. Tahapan perlakuan meliputi perendaman pucuk daun di dalam air dingin selama 3 jam, fiksasi dalam larutan Carnoy selama ±24 jam, dan hidrolisis dalam larutan HCl 5N selama 30 menit. Hasil penelitian menunjukkan nilai IM tertinggi meristem pucuk daun H. rosa-sinensis variasi single pink besar muncul pada pukul 10:00 sebesar 94%. Berdasarkan hasil tersebut dapat disimpulkan bahwa waktu sampling yang optimal untuk analisis kromosom H. rosa-sinensis L. variasi single pink besar adalah pukul 10:00 dengan jumlah kromosom 2n= ca. 69-111.  Hasil penelitian ini dapat dijadikan dasar untuk studi kromosom selanjutnya serta acuan untuk sampling variasi lainnya.Abstract Hibiscus rosa-sinensis L. is an ornamental plant that has many benefits and represents the character of polyploidy. The purpose of this study is to find out the Mitotic Index of leaf shoots Hibiscus rosa-sinensis on several shoots sampling times. The Mitotic Index and the timing of shoots sampling time are very necessary for chromosome studies because at this stage chromosomes characters can be clearly observed and easily calculated. Period time of collection the leaf shoots is from 08:00 AM to 16:00 PM, with two hours interval each at 08:00, 10:00, 12:00, 14:00, 16:00 . The chromosome preparation was carried out by using the squash method and aceto-orcein staining. The treatment steps included soaking the leaf shoots in cold water for 3 hours, fixation in Carnoy solution for ± 24 hours, and hydrolysis in 5N HCl solution for 30 minutes. The results showed that chromosomes were clearly visualized during the phase with the highest Mitotic Index.  In addition, the percentage of Mitotic Index was found to be in line with the percentage of cells in late prophase. Among several sampling time variations, the highest Mitotic Index of Hibiscus rosa-sinensis leaf shoots appeared at 10:00 at 94% with the chromosome numbers of 2n= ca. 69-111. According to the data obtained, it is concluded that 10 AM is the most optimum sampling time that can be used as the basic information for further chromosome studies.
Variasi Jumlah Kromosom Planlet Taraxacum officinale Weber ex FH. Wigg Hasil Regenerasi in vitro dari Eksplan Akar, Helai Daun dan Tangkai Daun Ermayanti, Tri Muji; Lestari, Indah; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.648 KB) | DOI: 10.14203/jbi.v10i2.2081

Abstract

Taraxacum officinale Weber ex F. H. Wigg. is a herbaceous medicinal plant species belonging family Asteraceasewhich has apomictic and poliploid characteristics. Multiplication of shoots using tissue culture was used to obtaindefine high quality seedlings, uniform, stable or free of diseases. However, changes in chromosome number canoccur in regenerated plants. The research aim was to determine the chromosome number of T. officinale plants regeneratedfrom culture in vitro using explants of roots, petioles and leaf blades. Therefore, selection of regenerantscan be done in order to find out transplants which have high yield of secondary metabolites. Analysis of chromosomenumber from root tips samples was conducted using 24 plantlets regenerated from root, 27 plantlets regeneratedfrom leaf blade, 21 plants regenerated from petiole and 102 roots of grown seeds using orcein squash method.The results showed that germinating seeds (control) and regenerated plants had variation in chromosome number.The range of chromosome numbers from regenerated plants were 2n=8-39, and cells with diploid number (2n = 2x= 16) was as most observed. The range numbers in germinated seeds were 2n=10-38, and cells with triploid number(2n = 3x = 24) was as most observed. This results proved that variation in numbers of chromosome was caused byapomixis and poliploid characteristics of the parent plant regenerated to their regenerants.Keywords : Taraxacum officinale. Weber ex F. H. Wigg, in vitro regeneration, variasi, chromosome
Pengaruh Aplikasi Bacillus sp. 140-B dan Streptomyces sp. L.3.1-DW Terhadap Infeksi Fusarium oxysporum Schlecht f. sp. cubense (FOC) dan Pertumbuhan Tanaman Pisang (Musa acuminata) var. Cavendish Laili, Nur; Antonius, Sarjiya; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3660.313 KB) | DOI: 10.14203/jbi.v11i1.2157

Abstract

Fusarium oxysporum Schlecht f. sp. cubense (Foc) is the causal pathogen of wilt disease of banana. Abilities of  biocontrol agents Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW to control Foc infection in banana were studied. Application of Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as single isolate or their combination in banana were tested under greenhouse conditions for 30 days. The aims of this study were to evaluate the potential of  Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as biocontrol agents in banana. Treatments of biocontrol showed significant effect on the reduction of foc infection diseases of banana, compared to control. The lowest disease severity was found on the treatment of single isolate Streptomyces sp.L.3.1-DW with infection degree of 29,33%. Streptomyces sp. L.3.1-DW could suppress Foc population (6,25 x 105 CFU/ml) in rhizosphere area after 30 days innoculation. Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW were also act as plant growth-promoting rhizobacteria (PGPR), that indicated by improvement of banana growth, in which Streptomyces L.3.1-DW caused the highest growth of banana either with or without Foc infection.  This study indicated that Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW have potential as alternative solutions to control Fusarium wilt in banana var. Cavendish. Keywords: Bacillus sp. 140-B, banana, biocontrol, Foc, PGPR, Streptomyces sp. L.3.1-DW. 
VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis] Sulistiani, Sulistiani; Mangunwardoyo, Wibowo; Abinawanto, Abinawanto; Sukara, Endang; Dinoto, Achmad; Salamah, Andi
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3242.558 KB) | DOI: 10.14203/beritabiologi.v16i2.2772

Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.
Akumulasi Timbal (Pb) dan Kadmium (Cd) pada Eclipta prostrata, Synedrella nodiflora, dan Tridax procumbens yang Tumbuh Liar Di Sisi Jalan Raya Bogor Rahmadina, Amara; Yuniati, Ratna; Salamah, Andi
BIOEDUSCIENCE Vol 3 No 1 (2019): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.132 KB) | DOI: 10.29405/j.bes/3123-323399

Abstract

Eclipta prostrata, Synedrella nodiflora, dan Tridax procumbens ditemukan tumbuh liar di sisi Jalan Raya Bogor. Penelitian dilakukan untuk mengetahui konsentrasi Pb dan Cd pada tanah, akar, dan tunas (aerial parts) E. prostrata, S. nodiflora, dan T. procumbens yang tumbuh liar di sisi Jalan Raya Bogor, serta untuk mengetahui mekanisme fitoremediasi pada ketiga tanaman tersebut sebagai kandidat fitoremediator. Analisis kandungan Pb dan Cd pada tanah dan tanaman dilakukan dengan Spektrofotometri Serapan Atom (SSA). Konsentrasi Pb dan Cd di tanah berkisar dari 2,72-103,38 mg/kg untuk Pb dan tidak terdeteksi hingga 0,08 mg/kg untuk Cd. Konsentrasi Pb tertinggi pada akar E. prostrata, S. nodiflora dan, T. procumbens berturut-turut 17,79 mg/kg, 14,53 mg/kg, dan 62,14 mg/kg, sedangkan pada tunas berturut-turut 4,91 mg/kg, 8,84 mg/kg, dan 6,08 mg/kg. Konsentrasi Cd tertinggi pada akar E. prostrata, S. nodiflora dan, T. procumbens berturut-turut 0,56 mg/kg, 0,23 mg/kg, dan 0,26 mg/kg, sedangkan pada tunas berturut-turut 0,29 mg/kg, 0,25 mg/kg, dan 0,45 mg/kg. Berdasarkan nilai Translocation Factor (TF), Bioconcentration Factor (BCF) dan Bioaccumulation Factor (BAF) pada ketiga tanaman tersebut, mekanisme yang cenderung digunakan E. prostrata, S. nodiflora, dan T. procumbens untuk penyerapan Pb, serta E. prostrata dan S. nodiflora untuk penyerapan Cd adalah fitostabilisasi, sedangkan  T. procumbens terhadap logam Cd berpotensi digunakan untuk fitoekstraksi
PENGARUH APLIKASI BACILLUS SP. 140-B DAN STREPTOMYCES SP. L.3.1-DW TERHADAP INFEKSI FUSARIUM OXYSPORUM SCHLECHT F. SP. CUBENSE (FOC) DAN PERTUMBUHAN TANAMAN PISANG (MUSA ACUMINATA) VAR. CAVENDISH Laili, Nur; Antonius, Sarjiya; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2157

Abstract

Fusarium oxysporum Schlecht f. sp. cubense (Foc) is the causal pathogen of wilt disease of banana. Abilities of  biocontrol agents Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW to control Foc infection in banana were studied. Application of Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as single isolate or their combination in banana were tested under greenhouse conditions for 30 days. The aims of this study were to evaluate the potential of  Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW as biocontrol agents in banana. Treatments of biocontrol showed significant effect on the reduction of foc infection diseases of banana, compared to control. The lowest disease severity was found on the treatment of single isolate Streptomyces sp.L.3.1-DW with infection degree of 29,33%. Streptomyces sp. L.3.1-DW could suppress Foc population (6,25 x 105 CFU/ml) in rhizosphere area after 30 days innoculation. Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW were also act as plant growth-promoting rhizobacteria (PGPR), that indicated by improvement of banana growth, in which Streptomyces L.3.1-DW caused the highest growth of banana either with or without Foc infection.  This study indicated that Bacillus sp. 140-B and Streptomyces sp. L.3.1-DW have potential as alternative solutions to control Fusarium wilt in banana var. Cavendish. Keywords: Bacillus sp. 140-B, banana, biocontrol, Foc, PGPR, Streptomyces sp. L.3.1-DW. 
VARIASI JUMLAH KROMOSOM PLANLET TARAXACUM OFFICINALE WEBER EX FH. WIGG HASIL REGENERASI IN VITRO DARI EKSPLAN AKAR, HELAI DAUN DAN TANGKAI DAUN Ermayanti, Tri Muji; Lestari, Indah; Salamah, Andi
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2081

Abstract

Taraxacum officinale Weber ex F. H. Wigg. is a herbaceous medicinal plant species belonging family Asteraceasewhich has apomictic and poliploid characteristics. Multiplication of shoots using tissue culture was used to obtaindefine high quality seedlings, uniform, stable or free of diseases. However, changes in chromosome number canoccur in regenerated plants. The research aim was to determine the chromosome number of T. officinale plants regeneratedfrom culture in vitro using explants of roots, petioles and leaf blades. Therefore, selection of regenerantscan be done in order to find out transplants which have high yield of secondary metabolites. Analysis of chromosomenumber from root tips samples was conducted using 24 plantlets regenerated from root, 27 plantlets regeneratedfrom leaf blade, 21 plants regenerated from petiole and 102 roots of grown seeds using orcein squash method.The results showed that germinating seeds (control) and regenerated plants had variation in chromosome number.The range of chromosome numbers from regenerated plants were 2n=8-39, and cells with diploid number (2n = 2x= 16) was as most observed. The range numbers in germinated seeds were 2n=10-38, and cells with triploid number(2n = 3x = 24) was as most observed. This results proved that variation in numbers of chromosome was caused byapomixis and poliploid characteristics of the parent plant regenerated to their regenerants.Keywords : Taraxacum officinale. Weber ex F. H. Wigg, in vitro regeneration, variasi, chromosome
DEPOSISI CALLOSE HASIL INDUKSI LIPOPOLISAKARIDA BAKTERI Pseudomonas syringae pv. tabaci PADA TANAMAN TEMBAKAU Marianingsih, Pipit; Salamah, Andi; Ichinose, Yuki
Prosiding Seminar Nasional MIPA 2012: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2012
Publisher : Prosiding Seminar Nasional MIPA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAKCallose, β-1,3-glukan, merupakan senyawa alami yang terdapat pada tanaman dan terlibat dalam sistem pertahanan tanaman. Callose merupakan bentuk pertahanan tanaman yang penting karena dapat menjadi penghalang awal masuknya patogen ke dalam tanaman. Deposisi callose termasuk ke dalam sistem pertahanan aktif karena akan terinduksi jika tanaman terserang patogen. Molekul patogen yang dilaporkan dapat menginduksi deposisi callose adalah lipopolisakarida (LPS), komponen utama membran luar bakteri gram negatif. Telah dilakukan penelitian untuk mempelajari induksi deposisi callose pada tanaman tembakau oleh LPS yang diekstraksi dari bakteri Pseudomonas syringae pv. tabaci (Pta). Bakteri Pta merupakan fitopatogen penyebab penyakit wildfire pada tembakau. Untuk pengamatan deposisi callose, daun tembakau diinfiltrasi dengan LPS Pta 400 μg/ml dan 800 μg/ml serta diinkubasi selama 24 jam dan 48 jam. Selanjutnya, klorofil daun diluruhkan menggunakan larutan laktofenol dan daun diwarnai dengan aniline blue. Hasil pengamatan menunjukkan deposisi callose yang telah diwarnai dengan aniline blue tampak berpendar warna hijau ketika diamati menggunakan mikroskop fluoresensi. Intensitas deposisi callose, hasil induksi LPS bakteri Pta, dalam satu daun tampak tidak merata di semua bagian daun, namun dan cenderung terdeposisi di sekitar pertulangan daun.