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All Journal Microbiology Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Borobudur
Ariyanti Oetari
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Agriculture, Biological Sciences & Forestry Arts Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Penapisan Aktivitas Selulase Isolat-isolat Khamir dari Moluska, Serasah, dan Tumbuhan di Taman Nasional Gunung Halimun, Jawa Barat Wibowo Mangunwardoyo; Reno Fitri; Ariyanti Oetari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v12i3.2805

Abstract

A total of 236 yeast isolates from mollusc, litter, and plant samples from Gunung Halimun National Park were screened for cellulolytic activity based on Smith method by using 0,2% (w/v) cellulose-azure for 30 days. The results showed that 12 isolates (9 isolates from plants, 2 isolates from molluscs, dan 1 isolate from litter) have cellulolytic activity. These isolates were further screened based on Teather and Wood method for six days to determine their cellulases components by using specific substrates. Carboxymethyl cellulose (CMC) 0,1% (w/v) was used as a specific substrate to determine endoglucanase activity. Avicel 0,1% (w/v) was used as a specific substrate to determine exoglucanase activity. Cellobiose 0,1% (w/v) was used as a specific substrate to determine β-glucosidase activity. The results showed that 6 isolates from plant have β-glucosidase activity, and 1 isolate from plant have β-glucosidase and endoglucanase activities. Five isolates (2 isolates from plants, 2 isolates from molluscs, and 1 isolate from litter) showed no cellulase activity on specific substrates after six days incubation.
Ecological and Taxonomical Perspective of Yeasts in Indonesia WELLYZAR SJAMSURIDZAL; ARIYANTI OETARI; ATIT KANTI; RASTI SARASWATI; CHIHARU NAKASHIMA; YANTYATI WIDYASTUTI; ANDO KATSUHIKO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8632.042 KB) | DOI: 10.5454/mi.4.2.3

Abstract

In the course of ecological and taxonomical study of yeasts in Indonesia, a total of 2147 yeast isolates from 315 samples in the year 2003, 2005, 2006, and 2008 had been obtained from eight locations e.g. Liwa (Sumatera), Cibinong (Java), Cibodas (Java), Kutai (Kalimantan), Enrekang (Sulawesi), Pucak (Sulawesi), Gili and Kuta (Lombok), and Kupang (Timor). Leaves, flowers, litters, soils, epiphytic soils, insects and insect's nests were collected for yeasts isolation. Our molecular identification based on D1/D2 region of nuclear large-subunit rDNA and the internal transcribed spacer (ITS) regions sequence data on 525 representative isolates revealed that 306 isolates belong to 48 described species (18 genera) and 209 strains belong to 19 undescribed species (19 genera), and 10 isolates were discarded because of contamination. Based on their substrates, litter had the highest yeasts genera (19) followed by soils (18), flowers (10), leaves (6), epiphytic soils (4), and insects and insect's nests (4). Genera found on soils were also common on litters. Yeasts genera found on flowers and epiphytic soils were common on leaves and litters. The genera Aureobasidium, Cryptococcus, Pseudozyma, Rhodotorula and Sporidiobolus were found in all substrates. Based on their locations, Kutai had the highest number of genera (15) followed by Cibodas (10), Cibinong (10), Enrekang (10), Kupang (10), Pucak (9), Liwa (7), and Lombok (7). The genus Cryptococcus was found in all locations. Our study shed a light to detection of many new taxa of yeasts, 41% of yeasts found in this study represented novel taxa.
Identifikasi Penyebab Kerusakan Biologis Gambar Cadas Gua Prasejarah Maros, Sulawesi Selatan Moh Habibi; Ariyanti Oetari; R. Cecep Eka Permana
Borobudur Vol. 14 No. 1 (2020): Jurnal Konservasi Cagar Budaya Borobudur
Publisher : Balai Konservasi Borobudur Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33374/jurnalkonservasicagarbudaya.v14i1.229

Abstract

Penelitian ini bertujuan untuk mengidentifikasi mikroorganisme (kapang dan bakteri) yang terdapat pada gambar cadas dan berpotensi sebagai biodeteriogen gambar cadas. Metode penelitian meliputi pengambilan sampel dan identifikasi molekuler. Identifikasi molekuler mengunakan primer 9F (5’-GAG TTT GAT CIT IGC TCAG-3’) dan 1510R (5’-GGT TAC CTT GTT ACG ACTT-3’) untuk bakteri, ITS1F (5’-CTT GGT CAT TTA GAG GAA GTAA-3’) dan ITS4R (5’-TCC TCC GCT TAT TGA TAT GC-3’) untuk kapang. Siklus PCR yang digunakan pada penelitian ini sebanyak 35 siklus, meliputi Denaturasi (95oC selama 15 detik), annealing, dan elongation (72oC selama 10 detik). Berdasarkan hasil identifikasi molekuler di peroleh tiga belas jenis mikroorganisme yang terdiri dari empat jenis Bakteri dan sembilan jenis Kapang.
Co-Authors ANDO KATSUHIKO Atit Kanti CHIHARU NAKASHIMA Gatot F Hertono Moh Habibi R. Cecep Eka Permana RASTI SARASWATI Reno Fitri Sitaresmi . Wellyzar Sjamsuridzal Wibowo Mangunwardoyo Yantyati Widyastuti