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Ni Putu Desy Aryantini
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Eksopolisakarida dari Lactobacillus sp. Isolat Susu Kuda Sumbawa dan Potensinya sebagai Prebiotik (EXOPOLYSACCHARIDES FROM LACTOBACILLUS SP. ISOLATED FROM SUMBAWA MARE’S MILK AND ITS POTENTIAL APPLICATION AS PREBIOTICS) I Nengah Sujaya; Ni Putu Desy Aryantini; Ni Wayan Nursini; Cok. Istri Dewiyani Cakrawati; Ni Luh Made Ema Juliasari; Ni Made Utami Dwipayanti; Yan Ramona
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research aimed to isolate exopolysaccharides (EPS) producing Lactobacilli isolated fromsumbawa mare’s milk and its potential as prebiotics for modulating the growth of Bifidobacteriumbreve. Nine strains of Lactobacillus sp. were screened for their capabilities to produce EPS usingmodified MRS medium containing sucrose. Prebiotics potential of the EPS was verified by culturingB. breve JCM1273 in TOS medium containing EPS. The results showed that all strains ofLactobacillus sp. produced EPS on MRS sucrose medium and two strains (Lactobacillus SK3.1 andLactobacillus SK4) produced more EPS compared to the other strains tested. Bifidobacteriumbreve JCM1273 showed weak activity while in direct metabolism of EPS produced by Lactobacillussp. SK4 and its growth was enhanced on acid hydrolyzed EPS. Since this phenomenon mighthappened when the EPS exposed by the low pH during gastric passage, hence the EPS might be apotential source to be developed as prebiotics. Nevertheless, further investigation is necessary toevaluate the bifidogenic affects of EPS in Lactobacillus sp. SK4.
Identifikasi dan Karakterisasi Bakteri Asam Laktat Isolat Susu Segar Sapi Bali (IDENTIFICATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM BALI CATTLE’S RAW MILK) I Nengah Sujaya; Komang Ayu Nocianitri; Ni Putu Desy Aryantini; Wayan Nursini; Yan Ramona; Yoshitake Orikasa; Fukuda Kenji; Tadashu Urashima; Yuji Oda
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bali cattle is an indigenous spesies in Bali, which pay great attention due to its uniqueness. Numerousarticles have been published on Bali cattle especially related to its disease, nutritional requirement forgrowth and domestication. Nevertherless, it was no any report has been published on the lactic acidbacteria (LAB) assosiated with the cattles raw milk and its potential used as probiotic. This work isaimed to identify LAB isolated from bali cattle raw milk and its resistance to secondary bile acid (sodiumdeoxy cholic), a prequisite in development of probiotic for human. The results revealed that based upon thehomology studies of the variable region I, II, and III sequences of the 16S rDNA showed that 44 out of 62isolates were closely related to Pediococcus acidilactici; 11 out of 62 isolats were closely related to Enterococusgallinarum, five out of 62 isolates were closely related to Lactococcus garvieae, while only one isolate was closely related to Lactobacillus plantarum and Weisella confusa. Some isolates showed resistant to 0.2-0.6mM deoxy cholic acid, which might be also resist in human gastrointestinal tract conditions. Based onthose finding, it can be concluded that the LAB associated with raw bali cattle milk were closey related toP. acidilactici, E. gallinarum, Lac. garvieae, Lb. plantarum and W. confusa, which different from thosecommonly LAB found in others cattle raw milk. Somes isolates were potential to be developed as probioticfrom human helath.
Co-Authors Cok. Istri Dewiyani Cakrawati Fukuda Kenji I Nengah Sujaya KOMANG AYU NOCIANITRI Ni Luh Made Ema Juliasari Ni Made Utami Dwipayanti Ni Wayan Nursini Tadashu Urashima Yan Ramona Yoshitake Orikasa Yuji Oda