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All Journal HAYATI Journal of Biosciences Indonesian Journal of Biotechnology BIOPROSPEK
Pudji Astuti
Department Of Physiology, Faculty Of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Veterinary

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The Potency of Anadara nodifera Shell as Natural Testosterone Booster for Male Canary (Seriunus canaria) Pudji Astuti; Muhammad Naufal Prayitno Putra; Muhammad Fariz Ash Shiddiq; Rizki Fitrawan Yuneldi; Claude Mona Airin; Sarmin Sarmin
HAYATI Journal of Biosciences Vol. 29 No. 1 (2022): January 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.1.107-113

Abstract

Anadara clam shell contains high Zinc. This microelement is recognised as acting natural aromatase blocker (NAB) that capable of boosting testosterone level. The aim of this study was to evaluates the testosterone level of Canaries following Anadara nodifera administration. Adult male Canaries (n = 27) were group into 3 with 9 repetition of each i.e T0 [control, was given by Carboxymethyl Cellulose Sodium (Na-CMC)], T1 (Zinc Sulphate 0.013 mg/30 g BW), and T2 (NAB 0.3 mg/30 g BW). The T2 treatment was NAB or shell powder of Anadara nodifera which was diluted 1:1 in Na-CMC. The birds were treated orally for 21 days. The complete blood cell count was performed in a haemo analyser except the erythrocyte was calculated manually in the Improved Neubauer counting chamber. Testosterone level was measured in ELISA. Subsequently gen CYP19 aromatase in syrinx, brain and testis were identified employing IHC and descriptively analysed. The result indicated significant elevated level of testosterone (p<0.05). The expression of CYP19 aromatase receptor was depressed in syrinx, brain and testis. However there was no significant changes on the blood profile (p>0.05). To conclude, Anadara nodifera at 0.3 mg/30 g BW was capable of acting as NAB to promote testosterone level. This was confirmed by the reduction of CYP19 aromatase gene receptors in syrinx, brain and testis. However NAB treatment was not significantly affecting routine blood profile.
The effect of methanol extract of soybean seeds (Glycine max L.Merr.) on the histology and immunohistochemical distribution of Cyp19 aromatase in rat testis (Rattus norvegicus L.) Retno Aryani; Sukarti Moeljopawiro; Laurentius Hartanto Nugroho; Pudji Astuti
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (839.696 KB) | DOI: 10.22146/ijbiotech.24199

Abstract

Soybean (Glycine max L. Merr.) contains phytoestrogens that have a chemical structure resembling estrogen in the body. They function like estrogen and antiestrogen, affecting the metabolism of sex steroid hormones. This research aimed to determine the effect of the methanol extract of soybean on the histological structure and distribution of immunohistochemical Cyp19 aromatase in rat testis . Twenty males of Wistar rats were divided into 4 groups of 5. The frst group was the control and the second to fourth groups were given soybean extract (250 mg/kg of body weight, 500 mg/kg of body weight) and genistein (0.3 mg/kg of body weight), respectively, for 52 days. The results of this study indicate that the effect of methanol extract from soybean caused weight gain, and the weight of the testis and epididymis decreased. In addition, the histological results showed that seminiferous tubules were reduced in size, became irregular, were separated by a wide interstitium, and spermatogenic cells were decreased. The immunohistochemical results showed that the expression of Cyp19 aromatase in the rats decreased both in spermatocyte cells and Leydig cells. It could be concluded that the methanol extract of soybean induced testicular damage and reduced Cyp19 aromatase expression in rat testis.
Penetapan Kadar Genistein Dalam Ekstrak Metanol BijiKedelai Glycine Max L. Merr. Varietas Grobogan MenggunakanMetode KLT Dan HPLC Retno Aryani; Pudji Astuti; Soekarti Moeljopawiro; Laurentius Hartanto Nugroho
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 10 No 2 (2015): Bioprospek: Jurnal Ilmiah Biologi: Volume 10 Number 2 2015
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.279 KB)

Abstract

Soybean (Glycine max L. Merr.) is one of the foodstuffs that are oftenconsumed most of Indonesian. Soy contains phytoestrogens, whichhave chemical structures resemble the hormone estrogen in the body,namely the isoflavones, especially genistein. Genistein is known notonly have various beneficial physiological effects but also act as anendocrine disruptor is because estrogen can play a role as well asantiestrogen activity that affects the metabolism of sex hormones andrelated activities biologi. The objective of this study was to know thequality of genistein in soy extracts Grobogan varieties that have beenpeeled the husk with Thin Layer Chromatography (TLC) and thequantity of soy extract genistein levels Grobogan varieties that havebeen peeled the husk with a method of High Performance LiquidChromatography (HPLC). The method used for extraction ofisoflavones genistein is maceration. Soybean seed samples which havebeen hulled begins with removal of non polar compounds byextraction using n-hexane solvent extraction followed macerationusing 80% methanol. The methanol extract obtained fractionated witha stationary phase of silica gel GF 254, the mobile phase toluene-ethylacetate-aseton- formic acid 20: 4: 2: 1 (v / v). To determine whether ornot a compound genistein compounds in the extract by using acomparison standard genistein further Rf sample extract comparedwith the price of a standard Rf. While the determination of genisteincompound quantitatively with HPLC method genistein standard wear≄ 98% at a concentration of 2, 4, 6, 8, 10 ppm. The identification ofgenistein using Thin Layer Chromatography (TLC), shows the stainsthat have Rf of about 0.43. Results genistein content by HPLC showedpeaks of the chromatograms that have a retention time of about 2,050minutes. HPLC analysis results in three replication showed levels ofgenistein in soya bean varieties Grobogan was 1 g samples of soyextracts that have been flayed husk there was an average of 0.6356 mggenistein. In the soybean seed Grobogan varieties contains isoflavonesgenistein. Levels of genistein in 1 g of sample soy extracts Groboganvarieties that have been flayed epidermis was an average of 0.6356 mggenistein.
Co-Authors Claude Mona Airin L. Hartanto Nugroho Muhammad Fariz Ash Shiddiq Muhammad Naufal Prayitno Putra Retno Aryani Rizki Fitrawan Yuneldi Sarmin Sarmin Soekarti Moeljopawiro Sukarti Moeljopawiro