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The effect of methanol extract of soybean seeds (Glycine max L.Merr.) on the histology and immunohistochemical distribution of Cyp19 aromatase in rat testis (Rattus norvegicus L.) Retno Aryani; Sukarti Moeljopawiro; Laurentius Hartanto Nugroho; Pudji Astuti
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Soybean (Glycine max L. Merr.) contains phytoestrogens that have a chemical structure resembling estrogen in the body. They function like estrogen and antiestrogen, affecting the metabolism of sex steroid hormones. This research aimed to determine the effect of the methanol extract of soybean on the histological structure and distribution of immunohistochemical Cyp19 aromatase in rat testis . Twenty males of Wistar rats were divided into 4 groups of 5. The frst group was the control and the second to fourth groups were given soybean extract (250 mg/kg of body weight, 500 mg/kg of body weight) and genistein (0.3 mg/kg of body weight), respectively, for 52 days. The results of this study indicate that the effect of methanol extract from soybean caused weight gain, and the weight of the testis and epididymis decreased. In addition, the histological results showed that seminiferous tubules were reduced in size, became irregular, were separated by a wide interstitium, and spermatogenic cells were decreased. The immunohistochemical results showed that the expression of Cyp19 aromatase in the rats decreased both in spermatocyte cells and Leydig cells. It could be concluded that the methanol extract of soybean induced testicular damage and reduced Cyp19 aromatase expression in rat testis.

EVALUASI DIET KOLIN DAN METIONIN TERHADAP PERTUMBUHAN DAN EFISIENSI PAKAN IKAN MAS (Cyprinus carpio) (Evaluation of Dietary Choline and Methionine on The Growth Performance and Feed Indices of Common Carp (Cyprinus carpio)) Rahma Suci; Rudy Agung Nugroho; Retno Aryani; Hetty Manurung; Rudianto Rudianto
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 16, No 2 (2020): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.16.2.%p

Kolin dan metionin bermanfaat dalam imunitas, pertumbuhan, deposisi lipid dan protein pada ikan. Penambahan kolin dan metionin dalam pakan ikan mas (Cyprinus carpio) perlu dilakukan untuk meningkatkan pertumbuhan dan efisiensi pakan. Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan kolin dan metionin pada pakan terhadap pertumbuhan dan efektivitas pakan ikan mas. Sejumlah 1.350 ekor ikan (umur ±2 bulan, berat awal 25,29±0,10 gram, panjang 10,5±11,2 cm) dibagi menjadi tujuh kelompok perlakuan dan satu kelompok kontrol dengan masing-masing tiga pengulangan. Kelompok kontrol dan perlakuan diberi diet basal (5 kali sehari) dengan penambahan persentase (%) rasio kolin dan metionin berbeda. Kelompok D1 = tanpa kolin dan metionin (kontrol); D2-8 adalah kelompok ikan dengan penambahan formulasi kombinasi kolin dan metionin sebagai berikut: D2 (0%:0,15%), D3 (0,04%:0%), D4 (0,04%:0,15%), D5 (0,08%:0%), D6 (0,08%: 0,15%), D7 (0,12%:0%), D8 (0,12%: 0,15%) selama 12 minggu. Parameter pertumbuhan yaitu: berat akhir, pertambahan berat badan, pertambahan berat harian, laju pertumbuhan spesifik, rasio konversi pakan, efisiensi pakan, laju efisiensi protein, dan kelangsungan hidup ikan mas yang diukur pada akhir minggu ke 12. Hasil penelitian, menunjukkan bahwa nilai tertinggi berat akhir, pertambahan berat badan, pertambahan berat harian, laju pertumbuhan spesifik, rasio efisiensi protein, dan efisiensi pakan didapat pada ikan mas kelompok D8. Sementara rasio konversi pakan ikan kelompok D3 menunjukkan hasil yang lebih tinggi dari ikan kelompok lainnya. Kesimpulan penelitian adalah diet D8 dapat diterapkan dan bermanfaat untuk meningkatkan pertumbuhan ikan. Sementara diet D3 bermanfaat untuk menaikkan efisiensi pakan dan rasio konversi pakan ikan mas. Choline and methionine are beneficial in immunity, growth, lipid and protein deposition in fish. A supplementation of choline and methionine in Carp fish (Cyprinus carpio) feed should be performed to improve growth and feed efficiency. This study evaluated the importance of choline and methionine supplementation on the growth and feed efficiency. In total 1,350 fish (± 2 months old, initial weight 25.29 ± 0.10 g, length 10.5 ± 11.2 cm) were randomly divided into triplicates seven treatment groups and one control group. For 12 weeks, the control and treatment fish groups were given a basal diet (5 times per day) with a different percentage (%) ratio of choline and methionine. Namely: D1 = without choline and methionine (control); D2-8 are groups of fish with a combination of formulation and methionine as follows: D2 (0%: 0.15%), D3 (0.04%: 0%), D4 (0.04%: 0.15%), D5 (0.08%: 0%), D6 (0.08%: 0.15%), D7 (0.12%: 0%), D8 (0.12%: 0.15%). Growth parameters such as: final weight (FW), body weight gain (BWG), daily weight gain (DWG), specific growth rate (SGR), feed conversion ratio (FCR), feed efficiency (FE), protein efficiency rate (PER), and survival rate were determined at the end of the week 12. The results showed that optimum FW, BWG, DWG, SGR, PER, and FE were obtained in D8 group. Meanwhile, the FCR of D3 group was higher than other groups. In conclusion, D8 diet is beneficial to to increase fish growth, while D3 diet is useful for increasing FE and FCR of carp.

Hubungan Panjang Berat Dan Faktor Kondisi Relatif Lima Spesies Ikan di Sungai Suwi Muara Ancalong, Kutai Timur Rudy Agung Nugroho; Apri Pauci Florentino; Lariman Lariman; Retno Aryani; Rudianto Rudianto; Monica Kusneti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 2 (2021): June 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i2.3524

The length-weight relationship of fish and the condition factor provides an overview of the fish growth conditions in the river. The purpose of this study was to determine the length-weight relationship and factor condition of five fish species in the Suwi River wetlands. The method of catching fish used gill nets, ceiling nets, fishing rods, traps, and other nets. The research was conducted for to months in the Suwi river wetlands. The results the length-weight relationship of five fish were varied, Ompok bimaculatus had b value 2,93, Helostoma temminckii (b value 2.15), Mystus nemurus (b value 2,62), and Channa) micropeltes (b 2.61) which indicated a negative allometric growth pattern. Meanwhile Leptobarbus hoevenii (b 3.12) was a positive allometric growth pattern. Negative allometric indicates growth in length faster than weight growth, thus the physical shape looks flattened, while positive allometric indicates growth in fish length slower than growth in fish weight. Based on the relative weight (Wr) of Ompok bimaculatus 101.28 ± 19.13, Helostoma temminckii 101.05 ± 15.51, Mystus nemurus 100.95 ± 14.84, Channa micropeltes 101.09 ± 14.14, Leptobarbus hoevenii 103.71 ± 34.57. Condition factor above 100, indicates sufficient food availability or low predator density

Pembekalan Teknik Ekstraksi, Skrining, dan Uji Antimikrobia Bahan Alam bagi Guru-Guru SMA Di Kota Samarinda Eko Kusumawati; Rudy Agung Nugroho; Retno Aryani; Hetty Manurung; Yanti Puspita Sari
Jurnal Pemberdayaan Masyarakat Vol 6 No 1 (2021): Mei
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jpm.v6i1.4040

Sebagai salah satu faktor penting dalam pendidikan, guru harus meningkatkan pengetahuan, baik teoritis maupun keterampilan, seperti praktik laboratorium, terutama untuk guru biologi. Berdasarkan hal ini, tujuan program pengabdian masyarakat adalah untuk meningkatkan keterampilan teoritis dan praktis guru biologi SMA. Program pengabdian masyarakat ini diikuti oleh 30 guru dari berbagai SMA di Samarinda. Teori tentang ekstraksi, penyaringan, dan teknik uji antimikrobia dari sumber daya alam dilakukan di ruang kelas, sedangkan penggunaan rotary evaporator, freeze drying, skrining uji fitokimia, dan uji antimikrobia dilakukan di laboratorium yang dibantu oleh teknisi laboratorium. Setelah menerima program pengabdian masyarakat ini, nilai rata-rata kepuasan yang diberikan oleh para guru melalui kuisioner yaitu: (skala 5) sebanyak 49%, baik (skala 4) sebanyak 47%, cukup (nilai skala 3) ) sebesar 5% dan lebih kecil (skala nilai 2) sebesar 1%. Kesimpulan, kegiatan pengabdian ini berjalan dengan baik dan mempunyai manfaat baik bagi guru biologi SMA di Samarinda.

Pemberdayaan Masyarakat dalam Budidaya dan Pemanfaatan Maggot Lalat Tentara Hitam Nova Hariani; Eko Kusumawati; Retno Aryani; Sus Trimurti; Lariman
Jurnal Pemberdayaan Masyarakat Vol 7 No 1 (2022): Mei
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21067/jpm.v7i1.6266

After 2 years of pandemic which caused by corona virus and followed by Restrictions Towards Community Activities (PPKM) was issued by government, has caused negative impact on various sector of community life. The impact on economic sector of society was quite large. Hence, community economic wheels which disrupted by laid off / stay home policy, needs to be maintained by offering home business with low budget. One of community empowerment efforts that can be introduced to society is utilization of household waste / small scale / fresh market to keep maggot black soldier fly (BSF). This maggot BSF is kind of clean fly and not classified as disease vector, otherwise it has many benefits as chicken feed, fish feed, substitution for protein feed, bird feed etc. Maggot has become alternative protein as animal feed. After the participant satisfaction test was held, we got participant satisfaction average values, ranged from very good (rated as 5) 60.2 %, good (rated as 4) 38.6%, and fair (rated as 3) 1.2 %. Overall, all participants were greatly satisfied with our presentation about mentioned knowledge and technology.

Subchronic Toxicity Test of Indian Almond (Terminalia catappa) Leaves Water Extract on The Liver Histology of Mice (Mus musculus) Nugroho Rudy; Ni Cening Sri Puspa; Retno Aryani; Widha Prahastika; Rudianto; Hetty Manurung
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Background: Indian almond (Terminalia catappa) is used as an alternative medicine by the community to treat various diseases. However, limited research has been conducted on the subchronic toxicity of Indian almond water extracts on the liver. Present study aimed to determine the subchronic toxic effects of Indian almond leaf water extract on the liver histology of mice and to evaluate the safe dose of Indian almond leaf water extract with various doses (125, 250, 500, 750 and 1000 mg/kg). Methods: In total of 33 male mice were randomly divided into 11 groups, each group consisting of three mice as replication. For 30 days, mice in five treatment groups were orally treated every day with water extracts of green Indian almond leaf while other five groups were given water extracts of brown Indian almond leaves and compare with control group. At the end of day 30, all mice were sacrificed, and hepar was taken out for histological preparations. The histopathological changes in the liver was observed and assessed based on histological damage in the term of degeneration and necrosis. Results: The administration of green and brown Indian almond water extract at doses of 125, 250, 500, 750 and 1000 mg / kg BB showed a toxic effect on the liver of mice. The toxic effect was due to the high doses, causing a cell swelling damage and increased liver necrosis. Conclusion: Indian almond leaf water extract can cause toxic effects on the liver of mice at the doses implemented.

Penetapan Kadar Genistein Dalam Ekstrak Metanol BijiKedelai Glycine Max L. Merr. Varietas Grobogan MenggunakanMetode KLT Dan HPLC Retno Aryani; Pudji Astuti; Soekarti Moeljopawiro; Laurentius Hartanto Nugroho
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 10 No 2 (2015): Bioprospek: Jurnal Ilmiah Biologi: Volume 10 Number 2 2015
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.279 KB)

Soybean (Glycine max L. Merr.) is one of the foodstuffs that are oftenconsumed most of Indonesian. Soy contains phytoestrogens, whichhave chemical structures resemble the hormone estrogen in the body,namely the isoflavones, especially genistein. Genistein is known notonly have various beneficial physiological effects but also act as anendocrine disruptor is because estrogen can play a role as well asantiestrogen activity that affects the metabolism of sex hormones andrelated activities biologi. The objective of this study was to know thequality of genistein in soy extracts Grobogan varieties that have beenpeeled the husk with Thin Layer Chromatography (TLC) and thequantity of soy extract genistein levels Grobogan varieties that havebeen peeled the husk with a method of High Performance LiquidChromatography (HPLC). The method used for extraction ofisoflavones genistein is maceration. Soybean seed samples which havebeen hulled begins with removal of non polar compounds byextraction using n-hexane solvent extraction followed macerationusing 80% methanol. The methanol extract obtained fractionated witha stationary phase of silica gel GF 254, the mobile phase toluene-ethylacetate-aseton- formic acid 20: 4: 2: 1 (v / v). To determine whether ornot a compound genistein compounds in the extract by using acomparison standard genistein further Rf sample extract comparedwith the price of a standard Rf. While the determination of genisteincompound quantitatively with HPLC method genistein standard wear≥ 98% at a concentration of 2, 4, 6, 8, 10 ppm. The identification ofgenistein using Thin Layer Chromatography (TLC), shows the stainsthat have Rf of about 0.43. Results genistein content by HPLC showedpeaks of the chromatograms that have a retention time of about 2,050minutes. HPLC analysis results in three replication showed levels ofgenistein in soya bean varieties Grobogan was 1 g samples of soyextracts that have been flayed husk there was an average of 0.6356 mggenistein. In the soybean seed Grobogan varieties contains isoflavonesgenistein. Levels of genistein in 1 g of sample soy extracts Groboganvarieties that have been flayed epidermis was an average of 0.6356 mggenistein.

PELATIHAN BUDIDAYA MAGGOT BAGI KLASTER SAPI BERKAH USAHA BERSAMA, DESA TELUK PANDAN BONTANG, KALIMANTAN TIMUR Hetty Manurung; Imam Rosadi; Ari Susandy Sanjaya; Wulan Lyhig Ratna sari; Rudianto Rudianto; Rudy Agung Nugroho; Retno Aryani; Andy Nurcahyono
LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 6 No 2 (2022)
Publisher : Department of Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Andalas Kampus Limau Manis - Padang, Sumatera Barat Indonesia-25163

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/logista.6.2.102-107.2022

Faktor harga pakan terutama komponen protein merupakan salah satu komponen utama yang menjadi masalah karena mahal harganya. Oleh karena itu, para peternak umumnya mencari alternatif sumber protein lain, salah satunya adalah maggot atau larva lalat tentara hitam (Hermetia illucens). Tujuan Pengabdian kepada masyarakat ini adalah untuk memperkenalkan dan melatih budidaya maggot kepada kelompok peternak sapi klaster sapi berkah usaha bersama, desa Teluk Pandan Bontang, Kalimantan Timur untuk menghasilkan maggot yang berprotein tinggi. Metode yang dilaksanakan mulai dari tahap persiapan, pelaksanaan yang berupa ceramah, tanya jawab, dan praktik budidaya. Hasil pengabdian yaitu, anggota kelompok peternak sapi klaster sapi berkah usaha bersama, desa Teluk Pandan Bontang, Kalimantan Timur ikut aktif berpartisipasi dalam kegiatan pelatihan, adanya interaksi tanya jawab dan juga praktek mandiri budidaya maggot. Kesimpulan yang didapat dari kegiatan pengabdian ini adalah anggota kelompok peternak sapi klaster sapi berkah usaha bersama, desa Teluk Pandan Bontang, Kalimantan Timur telah mengenal dan mampu melakukan budidaya maggot yang akan digunakan sebagai sumber protein untuk menekan harga pakan dalam usaha peternakan. Kata kunci: maggot, peternak sapi, bontang ABSTRACT The price of feed, especially protein, is an important factor because it has an expensive price. For this reason, farmers usually search for other alternative protein sources, one of which is maggot/Black Soldier Fly larvae (Hermetia Illucens). The purpose of this community service is to introduce and train maggot cultivation to the cattle breeder community in a group of cow farmer clustered Teluk Pandan Village, Bontang City, East Kalimantan to produce high protein nutrient from maggot. The method implemented was began with preparation stage, implementation in the form of lectures, questions and answers, and cultivation practices. The results of the community service were that members of the group of cow farmer clustered Teluk Pandan Village, Bontang City, East Kalimantan actively participated in training activities, question and answer interactions, and performed the maggot cultivation. The conclusion obtained from this community service activity was that members of the group of cow farmer clustered Teluk Pandan Village, Bontang City, East Kalimantan are familiar with and able to cultivate maggot which will be used as a source of protein to reduce feed prices in the livestock business. Keywords: black soldier fly larvae, cow farmer, bontang

Pertumbuhan dan Kelangsungan Hidup Ikan Patin (Pangasius sp.) Setelah Pemberian Pakan Bersubstitusi Biji Ketapang (Terminalia catappa L.) Retno Aryani; Rudy Agung Nugroho; Windi Rosiana Dewi; Yanti Puspita Sari; Hetty Manurung; Rudianto Rudianto
Al-Kauniyah: Jurnal Biologi Vol 16, No 2 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.12800

AbstrakPenggunaan biji tumbuhan yang mengandung protein tinggi sebagai bahan substitusi tepung ikan dalam pelet telah beberapa dilakukan. Tujuan dari penelitian ini adalah untuk mengetahui pertumbuhan dan kelangsungan hidup ikan patin (Pangasius sp.) setelah pemberian pakan bersubstitusi biji Terminalia catappa L. selama 12 minggu sebanyak 120 ekor ikan yang diberi pakan variasi substitusi tepung ikan: 0% (kontrol), 10, 15, dan 20%. Pertambahan bobot ikan (BWG), penambahan berat harian (DWG), pertambahan bobot mingguan (AWG), laju pertumbuhan relatif harian (SGR), pertambahan panjang total (LG), konversi pakan (FCR), efisien pakan (FE), dan kelangsungan hidup (SR) diukur setiap minggu. Hasil penelitian menunjukkan bahwa substitusi biji 10–20% dibandingkan dengan kontrol tanpa substitusi tidak ada beda nyata dalam parameter BWG, DWG, AWG, SGR, LG, FE dengan nilai tertinggi pada perlakuan 15% (P2) dan FCR 20% (P3). Sementara, SR berkisar 83,33–100%. Biji Ketapang dapat digunakan sebagai substitusi tepung ikan antara 10–20% untuk pertumbuhan dan kelangsungan hidup ikan yang optimum.AbstractThe use of nut plant which contains high protein level as a substitution for fish meal in the diet of fish has been performed in some researches. This study was conducted to determine the growth and survival of catfish (Pangasius sp.) fed ketapang (Terminalia catappa L.) seeds substitution for fish meal in their test diet for 12 weeks. The aquarium contained 120 fish that fed various concentrations of substitution with ketapang seeds in the test diet, vis: 0% (control), 10, 15, and 20%. Fish weight gain (BWG), daily weight gain (DWG), weekly weight gain (AWG), relative daily growth rate (SGR), total fish length (Length gain), feed conversion (FCR), feed efficiency (FE), and survival (SR) were measured every week. The results showed that 10 to 20% ketapang seed substitution compare to control had no significant effect in term of BWG, DWG, AWG, SGR, Length gain. The highest FE was achieved at 15% substitution while FCR 20%. The SR showed the percentage ranges from 83.33–100%. This study concluded that ketapang seed can be used as a fish meal substitution from 10–20% for optimum growth and survival of catfish.

Subchronic Toxicity Test of Indian Almond (Terminalia catappa) Leaves Water Extract on The Liver Histology of Mice (Mus musculus) Nugroho Rudy; Ni Cening Sri Puspa; Retno Aryani; Widha Prahastika; Rudianto Rudianto; Hetty Manurung
BIOEDUSCIENCE Vol 4 No 2 (2020): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/425173

Background: Indian almond (Terminalia catappa) is used as an alternative medicine by the community to treat various diseases. However, limited research has been conducted on the subchronic toxicity of Indian almond water extracts on the liver. Present study aimed to determine the subchronic toxic effects of Indian almond leaf water extract on the liver histology of mice and to evaluate the safe dose of Indian almond leaf water extract with various doses (125, 250, 500, 750 and 1000 mg/kg). Methods: In total of 33 male mice were randomly divided into 11 groups, each group consisting of three mice as replication. For 30 days, mice in five treatment groups were orally treated every day with water extracts of green Indian almond leaf while other five groups were given water extracts of brown Indian almond leaves and compare with control group. At the end of day 30, all mice were sacrificed, and hepar was taken out for histological preparations. The histopathological changes in the liver was observed and assessed based on histological damage in the term of degeneration and necrosis. Results: The administration of green and brown Indian almond water extract at doses of 125, 250, 500, 750 and 1000 mg / kg BB showed a toxic effect on the liver of mice. The toxic effect was due to the high doses, causing a cell swelling damage and increased liver necrosis. Conclusion: Indian almond leaf water extract can cause toxic effects on the liver of mice at the doses implemented.

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