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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) International Journal of Renewable Energy Development Jurnal AgroBiogen Journal of Tropical Horticulture
Nindita, Anggi
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Energy

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Induksi Tunas pada Kotiledon dan Hipokotil Tanaman Jarak Pagar (Jatropha curcas L.) melalui Organogenesis Tak Langsung Iswari S Dewi; Anggi Nindita; Bambang S. Purwoko; Darda Efendi
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p89-96

Abstract

Propagation through tissue culture of plantspecies with rich secondary metabolites such as Jatrophacurcas L. is difficult to obtain. However, once established, itcan be used as one of the alternatives to supply uniformpropagules. The effects of auxin and cytokinin on theregulation of de novo woody plants shoot development havebeen studied through shoot induction, differentiation anddevelopment. The objective of this research was to identifyexplant and suitable culture media for in vitro shoot inductionthrough indirect organogenesis. Factorial experimentwas arranged in a completely randomized design, replicated20 times. The first factor was explants, i.e. cotyledons andhypocotyls. The second factor was MS media containingcombination of plant growth regulator IAA (0, 0.05, and 0.1mg/l) and BAP (0, 1.0, 2.0, 3.0 mg/l). The results of theexperiment showed that the fastest callus initiation wasachieved by MS + IAA 0.1 mg/l, i.e. 9.5 days after explantswere cultured. Shoots with leaves can be induced from bothcotyledons and hypocotyls. However, hypocotyls gave moreshoots and leaves than cotyledons when cultured in MS +IAA 0.1 mg/l + BAP 3.0 mg/l. Shoots obtain from hypocotylsand cotyledons were successfully rooted in MS mediumwithout any growth regulator.
The Study of Early Selection Method through Vigor and Viability Parameter in Citrus amblycarpa (Hassk) under Salinity and Drought Stress Condition Anggi Nindita; Choirul Umam; Siti Fatimah; Faiza Suwarno
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1505.466 KB) | DOI: 10.33089/jthort.v1i1.4

Abstract

The study was aimed to obtain information regarding the early selection method of Limau lime under saline and drought condition including the proper method for seed extraction, observation period in germination testing, and type of germination media. The experiment was conducted from March 2015 to January 2016 at the Laboratory of Seed Science and Technology, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. It consisted of four main experiments. The first experiment was the extraction method on Limaulime seed using distilled water, ash from the husk, talcum powder, and HCl 1%. The second experiment was germination testing of lime seed using three media i.e. sand, the combination of sand: soil (1:1) (v:v), and towel tissue. The third experiment has tested the vigor of Limau lime seeds in different saline conditions by using NaCl, i.e. 1500 ppm, 3000 ppm, and 4500 ppm. The fourth experiment, tested the vigor of Limau lime seeds in drought stress condition using PEG 6000, i.e. 0, - 0.75 bar, -1.5 bar, and -2.25 bar. The result of the first experiment showed that the treatments had no significantly different effect on all variables observed except for the seed moisture content. The second experiment revealed that the best germination media was elucidated from sand treatment and sand: soil (1:1) with the first and the second count at 20th and 29th day respectively. The result of the third and four experiment elucidated that the higher concentration of NaCl (Salinity stress condition) or PEG 6000 (drought stress condition), the smaller the germination percentage of Limau Lime’s seeds.
Co-Authors Ade Wachjar Ade Wachjar Adi Nugraha Widayatmo Andi Sasmito Bambang S. Purwoko Bambang Sapta Purwoko Choirul Umam Delima Ragil Kartika Desta Wirnas Efendi, Darda Elvita Dwi Jayaningsih Faiza Suwarno Faradila Median Rini Fitrah Ramadhan HAJRIAL ASWIDINNOOR Iswari S Dewi Iswari Saraswati Dewi Kartika, Delima Ragil Laksono Trisnantoro Leopold O. Nelwan Maryamah, Umi Nita Kartina Praptiningsih G.A Rima Margareta R. Gumelar Rini, Faradila Median Roy Hendroko Satriyo K Wahono Siti Fatimah Surjono H. Sutjahjo Surjono Hadi Sutjahjo Tony Liwang Umi Maryamah Umi Salamah Widayatmo, Adi Nugraha Willy Bayuardi Suwarno