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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrohorti Bulletin (e-Journal) Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Hortikultura Indonesia Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Tanaman Tembakau, Serat & Minyak Industri AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL BIOLOGI INDONESIA International Journal of Applied Biology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan ANNALES BOGORIENSES Journal of Tropical Crop Science Warta Penelitian Perhubungan Menara Perkebunan JURNAL BUDIDAYA PERTANIAN Jurnal Penelitian Tanaman Industri (Littri) Buletin Tanaman Tembakau, Serat & Minyak Industri Perbal : Jurnal Pertanian Berkelanjutan

Efendi, Darda

Agronomy And Horticulture Department, Faculty Of Agriculture, Bogor Agricultural University Jl. Meranti, IPB Darmaga Campus, Bogor 16680, Indonesia

Author-ID : 689363

Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Economics, Econometrics & Finance Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Transportation

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DEHIDRASI DAN PEMBEKUAN JARINGAN APEKS TEBU UNTUK PENYIMPANAN JANGKA PANJANG ROOSTIKA, IKA; DEWI LIMA WATI, RARA PUSPITA; EFENDI, DARDA
853-8212
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

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ABSTRAKTebu (Saccharum officinarum L.) merupakan tanaman yangdiperbanyak secara vegetatif. Kriopreservasi merupakan metode yangpaling sesuai untuk penyimpanan jangka panjang bagi tanaman yangdiperbanyak secara vegetatif. Dehidrasi dan pembekuan jaringan merupa-kan tahapan paling kritis yang menentukan keberhasilan kriopreservasi.Tujuan penelitian adalah untuk memperoleh durasi dehidrasi yang optimaldan metode pembekuan jaringan apeks tebu. Penelitian dilakukan diLaboratorium Kultur Jaringan, Kelompok Peneliti Biologi Sel danJaringan, Balai Penelitian dan Pengembangan Bioteknologi dan Sumber-daya Genetik Pertanian, Bogor pada Mei 2013 sampai Februari 2014.Untuk optimasi metode dehidrasi, apeks direndam dalam larutan PVS2(MS + gliserol 30% + etilen glikol 15% + dimetil sulfoksida 15% +sukrosa 0,4 M) selama 10, 20, 30, dan 40 menit. Untuk optimasi metodepembekuan, diujikan kombinasi perlakuan prakultur (dengan sukrosa 0;0,1; dan 0,3 M selama 5 hari) dan pemuatan dalam larutan LS (MS +gliserol 2 M + sukrosa 0,4 M) selama 0, 10, 20, dan 30 menit sebelumtahapan dehidrasi dan pembekuan jaringan di dalam nitrogen cair (-196 o C). Hasil penelitian menunjukkan durasi dehidrasi jaringan yangterbaik adalah 30 menit dalam larutan PVS2. Kombinasi perlakuanprakultur dengan sukrosa 0,3 M dan pemuatan dengan larutan LS selama10 menit merupakan metode terbaik untuk pembekuan jaringan. Persentasetumbuh sebelum dan setelah pembekuan dalam nitrogen cair berturut-turutadalah 100 dan 40%. Setelah kriopreservasi, biakan mampu tumbuhdengan tingkat multiplikasi tunas sekitar 10 tunas/eksplan. Metode yangdiperoleh pada penelitian ini berpeluang diterapkan untuk penyimpananplasma nutfah tebu dalam jangka panjang secara kriopreservasi daneliminasi patogen obligat secara krioterapi.Kata kunci: Saccharum officinarum L., apeks, dehidrasi, pembekuan,nitrogen cairABSTRACTSugarcane (Saccharum officinarum L.) is vegetatively propagatedplant. Cryopreservation is the most suitable method for long-termpreservation of vegetatively propagated plant. Dehydration and freezingare critical steps of successful cryopreservation so that it should beoptimized. The research aimed to obtain the optimal duration ofdehydration and freezing method of sugarcane apex tissues. Theexperiments were conducted at Tissue Culture Laboratory, Plant CellTissue Biology Group, Indonesian Center for Agricultural BiotechnologyandÂ GeneticÂ ResourcesÂ ResearchÂ andÂ DevelopmentÂ onÂ May2013âFebruary 2014. To optimize dehydration method, the tissues wereexposured in PVS2 solution (MS + 30% glycerol + 15% ethylene glycol +15% dimethyl sulphoxide + 0.4 M sucrose) for 10, 20, 30, and 40 minutes.To optimize freezing method, the combined treatment of preculture withsucrose (0, 0.1, dan 0.3 M) for 5 days and loading in LS solution (MS + 2M glycerol + 0.4 M sucrose) for 0, 10, 20, dan 30 minutes) were testedbefore dehydration for 30 minutes and freezing in liquid nitrogen (-196 o C).The best duration of dehydration was 30 minutes. The combined treatmentof preculture on 0.3 M sucrose and loading for 10 minutes was the bestmethod for tissues freezing. Percentage of regrowth before and afterfreezing in liquid nitrogen was 100 and 40% respectively. Aftercryopreservation, the cultures could grow with high shoot multiplicationrate about 10 shoots/explant. The method resulted in this study can beapplied for long-term storage of sugarcane germplasms by cryopreser-vation and (elimination of obligate pathogens by cryotherapy.Keywords: Saccharum officinarum L., apex, dehydration, freezing, liquidnitrogen.

The Analysis of Î²-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Sumiasih, Inanpi Hidayati; Poerwanto, Roedhy; Efendi, Darda; Agusta, Andria; Yuliani, Sri
International Journal of Applied Biology Vol 1, No 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

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Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially Î²-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of Î²-cryptoxanthin with Î²-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the Î²-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 Â°C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase Î²-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: Î²-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.

Somatic Embryo Germination of Jatropha curcas L in Presence of Sucrose and Poly Ethylene Glycol (PEG) Rudiyanto, Rudiyanto; Efendi, Darda; Ermayanti, Tri Muji
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Jatropha curcas L. is a potential source of a non-edible biofuel. Conventional propagation of J. curcas technique has some limitations. Somatic embryo can produce a large number of embryos and obtain a large number of plants all year round. Treatment of sucrose in combination with polyethylene glycol (PEG) was proven to enhance germination of somatic embryos in many plant species. The aim of the study was to investigate the effect of sucrose in combination of PEG on somatic embryo germination in J. curcas. Globular somatic embryos at 0.025-0.030 g fresh weight having 0.4-0.5 cm in diameter were grown on MS medium solidified with 3 g/l of Gelzan supplemented with sucrose at 20, 30, 40, and 50 g/l in combination with PEG at 0, 2.5, 5, 10, and 15%. Results showed that the best medium for germination of J. curcas somatic embryo cultures was MS medium supplemented with 20 and 30 g/l of sucrose in combination with 5% of PEG. The numbers of germinated embryos per clump had significant enhancement on those medium compared with the control (PEG free treatment) (2.65 to 5.65) and (2.55 to 5.50). In addition, those treatments resulted in the highest percentage of clumps forming germinated embryos (100%), with an average of normal germinated embryos at 94.163 and 96.065%. The addition of 40 and 50 g/l of sucrose in combination with 15% of PEG caused all embryos to fail at germinating.

The Potentials of Limau (Citrus amblycarpa Hassk. Ochse) as A Functional Food and Ornamental Mini Tree Based on Metabolomic and Morphological Approaches Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda
Journal of Tropical Crop Science Vol 4 No 2 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Limau (Citrus amblycarpa (Hassk.) Ochse) is one of the native Citrus species of West Java that is grown in the local backyard, thus is easily found in local cuisines. Limau has great potentials for use as a potted plant in home gardens for its aesthetic value and fragrance. The objective of this research was to describe the metabolites fingerprint and morphology of Limau as a potential functional and ornamental mini tree. Methanol leaf extracts from one-year-old Limau originated from Bogor were analyzed using untargeted Gas Chromatography Mass Spectrometry (GCMS). Methanol leaf extract was dominated by vitamin E and composed of other 18 metabolites including phytosterols, fatty acids and terpenes. The major portion of fruit extract consisted of 6-octadecenoic acid, palmitate and various fragrance compounds such as alpha sinensal, alpha limonene, beta citronellal, citronellol, and sabinene. Limau fruits are also a functional food due to its rich antioxidant and aromatic content in the fruits. The morphology of limau tree is suitable as a potted ornamentals or mini-fruits tree for its small crown, sparse branching with unifoliate aromatic leaves and small spines, and plentiful aromatics mini fruits with the diameter of around 2-4 cm.

Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA Efendi, Darda; Putra, Mirza R
Journal of Tropical Crop Science Vol 4 No 3 (2017): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

Somatic Embryogenesis of Melon (Cucumis melo L.) As Affected by Culture Media and Composition of Plant Growth Regulators Furqoni, Hafith; Efendi, Darda
Journal of Tropical Crop Science Vol 5 No 3 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Conventional production of melon hybrid seeds requires a long time. Propagation through plant tissue culture can be an alternative method to produce hybrid melon seedlings in order to fulfill the high demand for uniform seedlings. Our current study was aimed to determine the type of propagation media and the best concentration of picloram for the induction of somatic embryogenesis in melon from young seed explants. The study was expanded to examine the effective concentrations of two of auxins, 2-4 D and NAA, combined with BAP to induce somatic embryogenesis in melon using hypocotyl explants. The experiment was conducted at Plant Tissue Culture Laboratory, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. The experiment was arranged in a randomized complete block design with three replications. The first experiment tested three types of planting media (MS, B5, and WPM) and four levels of picloram concentration (0, 0.5, 1.0, 1.5 mg.L-1). The second experiment tested auxin (2,4-D and NAA) concentrations of 0, 1, 2, 3, 4, 5 mg.L-1 and two BAP concentrations, 0 and 1 mg.L-1. The first study showed that no somatic embryos were formed with the media types and picloram concentration tested; the seeds, however, germinated and formed callus. In the second study, there was an interaction between concentration levels of auxin (2,4-D and NAA) and BAP on induction of somatic embryos using hypocotyl explants. Somatic embryo formation can be induced with treatment of 1 mg.L-1 NAA + 0.1 mg.L-1 BAP and 2 mg.L-1 NAA + 0.1 mg.L-1 BAP. The highest yield of embryos formation was with the treatment of 1 mg.L-1 NAA + 0.1 mg.L-1 BAP at 0.53 embryos per explant. Keywords: embryogenesis, tissue culture media, melon, Picloram, 2,4 D, BAP

A Review of Root Pruning to Regulate Citrus Growth Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda
Journal of Tropical Crop Science Vol 6 No 01 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Root is the important plant organ hiding below the soil that serves as plant supporting structure, water and nutrient supplier and assimilates storage. Root growth occurs in turn with shoot part, forming repeated cyclic pattern because of the photosynthate competition. Root growth regulation is one of the promising practices to boost the yield of fruit tree, especially citrus that is economically important and highly demanded fruit. Root pruning varied based on the scale of intensity and plant growth stage, including air root pruning, knife root pruning and modern pruning using root pruner machine mounted to a tractor. Root pruning to induce the growth of more fi brous root system and to correct root deformation at seedling stage have potentials to improve post-transplant growth. Besides, it has a potential to replace the use of chemical plant growth regulator for a more environmentally friendly practice. The pruning of root impeded the canopy growth, altered the plant water status leading to fl ower promotion. In addition, it is also a promising practice to overcome the alternate bearing on fruit tree, including citrus, through the suppressing of excessive growth and restricting the high fruit load during the ‘on year’ and allowing a better carbohydrate storage for the improvement of yield during the ‘off year’.

Production, Post-Harvest and Marketing of Kaffir Lime (Citrus hystrix DC) In Tulungagung, Indonesia Budiarto, Rahmat; Poerwanto, Roedhy; Santosa, Edi; Efendi, Darda; Agusta, Andria
Journal of Tropical Crop Science Vol 6 No 02 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Kaffir lime (Citrus hystrix DC) is one of the Citrus species that is popular as food spices in Indonesia. This study was aimed at describing the production system, post-harvest handling, marketing and evaluating the product variation of kaffir lime cultivated in the Tulungagung district, East Java Province, Indonesia. Data was collected through a survey by interviewing kaffir lime farmers, distributors and customers in June 2018. Kaffir lime is cultivated by local farmers using traditional and conventional agriculture practices. The farmer used good quality seedlings as planting materials and applies animal manure or compost to the trees. Kaffir lime is grown on rain-fed land; fertilizers and pesticides are applied sparingly. The kaffir lime leaves could be harvested twice a year in different seasons. Better leaf quality can be obtained during the rainy seasons. The leaf production per tree is estimated at about 100 gram per plant per season. Post-harvest handling involved sorting, packing and marketing without any cooling treatment. The standard gate price of kaffir lime leaf in June 2019 was IDR 6,000-7,000, IDR 9,000-10,000 at the collector level, IDR 11,000-12,000 at wholesaler, and IDR 14,000-16,000 at small retailers. Leaves were distributed to traditional market in Tulungagung, neighbouring districts such as Kediri, Trenggalek and Blitar, and also to capital cities such Jakarta. The main product of kaffir lime is the fresh leaf for food seasoning and as a raw material to produce essential oil and dry leaf powder. The stems have been used for fuel in home industries. The fruits of kaffir lime are rarely found in the market and have great potentials for essential oil production and recently, a promising commodity for export.

Study of Incubation Duration After Degreening and Storage Methods on the Quality of Tangerine "Garut" (Citrus reticulata B.) Tiara, Dede; Poerwanto, Roedhy; Efendi, Darda
Journal of Tropical Crop Science Vol 6 No 03 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Ripened citrus fruits with an orange rind have a higher market demand relative to unripen citrus with a green rind. This research was aimed to evaluate the combination of post-degreening incubation duration before storage with the type of storage to increase orange rind color and storage life of citrus fruit. A randomized block design with two factors was used; the first factor was post-degreening incubation duration before storage that comprised of without incubation, incubation for two and four days. The second factor was storage method that comprised of storage at room temperature (29 ± 1oC), at 18 ± 1oC, waxing and storage at room temperature, waxing and storage at 18 ± 1oC. The result shows that post-degreening incubation duration that increased orange color of citrus rind was four days. The citrus which was stored at 18 ± 1oC had the smallest fruit weight loss. The best treatment combination which increased orange color formation and had lower percentage of fruit weight loss was a combination of 4 days incubation after degreening and storage at 18± 1oC.

PUSAT PENELITIAN BIOTEKNOLOGI-LIPI, JL. RAYA BOGOR KM. 46, CIBINONG, 16911, JAWA BARAT, INDONESIA Susetio, Muhammad; Efendi, Darda; Sari, Laela
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3761

ABSTRACTBentul taro (Colocasia esculenta) is one of taro cultivar which has high productivity. Genetic improvement has been done by obtaining tetraploid taro. Drought tolerant cultivars can be achieved by addition of poly ethylene glycol (PEG) in vitro. The purpose of this study was to determine the effect of the treatment of PEG concentrations on in vitro growth of tetraploid shoots and to propagate shoots after PEG treatment. The explants used were diploid taro shoots, tetraploid clones 2.1.2 and 2.4.2. Shoots were planted in MS media without PEG and with PEG of 5, 10 and 15%. The growth parameters observed were petiole length, number of leaves, and number of roots every week for 6 weeks. Proline content and LC50 values were analyzed at 6 weeks after planting. Propagation of shoots after PEG treatment was carried out on MS media containing 2 mg / L BAP, 1 mg / L thiamine, and 2 mg / L adenine. Each treatment consisted of 15 shoots as replicates. The growth parameters observed were petiole length, number of leaves, and number of shoots every week for 6 weeks. The results showed that Bentul taro clones significantly affected the concentration of PEG on growth parameters. LC50 value of diploid clone was 12.16%, clone 2.1.2 was 13.54%, and clone 2.4.2 was 12.74%. The highest proline content was found at Bentul tetraploid clone 2.1.2. After PEG treatment, growth was significantly affected by PEG concentrations. All Bentul taro clones after PEG treatment produced multiple shoots. Keywords: Taro (Colocasia esculenta), in vitro selection, diploid, tetraploid, proline, LC50, propagation

Co-Authors . Angela . Rudiyanto Ade Wachjar Ade Wachjar Alfia Annur Aini Azizi and Gustaaf Adolf Wattimena Andria Agusta Andria Agusta ANDRIA AGUSTA Angela, . Anneke Pesik Asti Kusriyanti Azizi, Alfia Annur Aini Azizi, Alfia Annur Aini Bambang S PURWOKO Bambang S. Purwoko Bambang S. Purwoko C Hanny Wijaya Cece Suhara dan Joko Ridho Witono Dede Robiatul Adawiyah Deden Derajat Matra Deden Sukmadjaja Dewi Sukma Dianto, Fajar Didy Sopandie DINARTY, DINY Diny Dinarti Djoko Santoso Djoko Santoso Don R LaBonte Dorly Dorly Dwi Utami Nur Usmani Dyra Haryanti E. Gunawan Edi Santosa Entit Hermawan Erwin Al-Hafiizh Evan Maulana Fajar Dianto Fajarudin, A Fitri Fatma Wardani Fitri Fatma Wardani Furqoni, Hafith Gunawan, E. Gustaaf A Wattimena Hanifah Muthmainnah heliyana hermawati Ika Mariska Ika Roostika IKA ROOSTIKA Ika Roostika Ika Roostika Ika Roostika Ika Roostika Tambunan Imanullah Dacholfany Imron Riyadi Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari Iswari S Dewi Joko Ridho Witono Jollanda Effendy Jollanda Effendy Kasutjianingati . Katerin Ninariyani Ketty Suketi Kusriyanti, Asti Laela Sari laela Sari, laela Lisnandar, Dea Silvia Lolliani MASKROMO, ISMAIL Maulana, Mohamad Akhbar Maya Melati Mayasari Yamin Mohamad Akhbar Maulana Mutiara Utami Naimatul Farida Ninariyani, Katerin Nindita, Anggi NOVARIANTO, HENGKY Nurul Khumaida Nurul Khumaida Nurul Khumaida Odit Ferry Kurniadinata Ogie Satriadi Putra, Mirza R Rahmat Budiarto Rahmi Fajri RARA PUSPITA DEWI LIMA WATI RARA PUSPITA DEWI LIMA WATI, RARA PUSPITA Reflinur Reflinur Resa Sri Rahayu Resa Sri Rahayu Riry Prihatini ROEDHY POERWANTO Rudiyanto Rudiyanto Rudiyanto Rudiyanto, Rudiyanto S Noorrohmah Satriadi, Ogie Sedyo Harsono SEDYO HARTONO Slamet Susanto Sobir Sobir Soekisman Tjitrosemito Sony Hartono Wijaya SRI HENDRASTUTI HIDAYAT Sri Yuliani Sri Yuliani Sri Yuliani Sudarsono Sukma, Dewi Sulassih, . Surya Diantina Susetio, Muhammad Tambunan, Ika Roostika Tambunan, Ika Roostika Tanari, Yulinda Taruna Shafa Arzam TENDA, ELSJE T. Tiara, Dede TRI ASMIRA DAMAYANTI Tri Istianingsih Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Trikoesoemaningtyas Triokoesoemaningtyas Triokoesoemaningtyas Vandra Kurniawan Wachjar, Ade Wahyu Fikrinda Wida W. Khumaero Willy Bayuardi Suwarno Winarso D. Widodo Yamin, Mayasari Yande Artha Gautama Yosi Zendra Joni Yosi Zendra Joni Yundari, Yundari

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