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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Biotropika Indian Journal of Forensic Medicine & Toxicology Journal of Global Pharma Technology
Muhaimin Rifa’i
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Journal of Global Pharma Technology

 1 
Potential Regulatory Functions of Propolis to Ameliorate Hematopoietic Disorder in Diabetic Mouse Model Muhaimin Rifa’i
Journal of Global Pharma Technology Volume 09 Issue 03
Publisher : Journal of Global Pharma Technology

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Abstract

Diabetes mellitus (DM) in autoimmune case, related to cytotoxic T cells activation as a response to autoantigen. In the recent studies, propolis is known as a potential immunomodulator. The aim of this study is to determine the effect of propolis as a regulator to ameliorate diabetic mouse model. In vivo experiment was done using streptozotocin-induced diabetic mice. There are five experiment groups; that are DM group (diabetic mice model without treatment); propolis ethanolic extract treatment group doses of 50, 100, and 200 mg/kg BW; and normal mice group. Effect of propolis was observed 14 days post treatment by flow cytometric analysis. The number of CD8+CD62L-, CD4+CD25+CD62L+(Treg), B220+,  and NK+ cells was assessed from splenic cells. The result showed that administration of propolis for 14 days could reduce the number of cytotoxic T lymphocytes (CTL) memory significantly (p ≤ 0, 05). Especially the doses of 100 and 200 mg/kg BW could suppress the activation of CTL quite similar to those normal groups. Propolis with the dose of 200 mg/kg BW increase the number of  Treg, B220, and NK cells. The increase of Treg may reduce the activation of CTL and prevent tissue damage by autoreactive cells, and finally hematopoietic imbalance can be overcome. Keywords: B220, diabetes mellitus, immunomodulatory, NK, propolis.
Effectiveness of Ethanolic Propolis Extract (EPE) Administration in Ameliorating Diabetic Mice Model Muhaimin Rifa’i
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

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Abstract

Diabetes mellitus (DM) is a group of metabolic disorder characterized by hyperglycemia resulting from defects in insulin action, insulin secretion, or both. Many efforts have been made to overcome this disease but until now has not been fully successful. This study aimed at examining CD34+, TER119+VLA4+, B220+  cell population,  and stromal cell-derived factor 1 (SDF-1) expression in an attempt to correct homeostasis indiabetic mice model with active disease.Three different EPE doses were tested (50, 100, and 200 mg/kg BW).  The oral administration of EPE was conducted for 14 days. On day 15, the bone marrow cells were isolated and then subjected to flow cytometry analysis, and data was processed by One-way ANOVA (p < 0.05) and Tukey test using SPSS 16.0 for Windows. The results showed that administration of EPE with the doses of 50 and 100 mg/kg BW) potentially increased cell proliferation. In contrast, the doses of 200 mg/kg BW seem to be in potentially suppressing cell proliferation. Taken together, propolis, which is involved in the induction of proliferation, show a rather in low doses, while high dose will give a suppression effect on cell proliferation and differentiation. Therefore, the use of propolis for treatment should consider the appropriate dose.  Keywords: B220, CD34, Diabetes mellitus, SDF-1, TER-119+VLA-4+.
Co-Authors Akhmad Fathir Aryati Haruhiko Suzuki Hideo Tsuboi Iswinarno Doso Saputro Izumi Nakashima Khaled Hossain Moch Sasmito Djati Mohammad Hefni Septin Mauludiyana Swastika Pinca Pinca Widodo - Yoes Prijatna Dachlan Yundari, Yundari Zumrotul Mufidah