Article Per Year (5 Year)
p-Index From 2019 - 2024
0.23
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner Biotropika Indian Journal of Forensic Medicine & Toxicology Journal of Global Pharma Technology
Muhaimin Rifa’i
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 9 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 9 Documents
Search

 1 
Immune Response to Burn Injury: Hyperinflammation and Immunosuppression Septin Mauludiyana; Aryati; Yoes Prijatna Dachlan; Iswinarno Doso Saputro; Muhaimin Rifa’i
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15936

Abstract

Burn is an injury to the skin or other tissue. Mostly, it caused by contacting with hot liquids, solids, and flames. The important thing that should be consider in burn incident is the severity of burn and it based on the depth and area of the burn injury. The severity of burns will cause differences of pathophysiological responses. This was a literature review study. Various articles were collected from online database including reports, journals, and published in the last 10 years. The articles were from the scholar journals. The systemic inflammatory response in severe level of burns was not given good response to disappear of lesion burn and initiating tissue repair. Moreover, it was given an organ failure to the patient. The body responded to this incident by releasing antiinflammatory mediators. This response is very strong and prolonged, so it caused immunosuppression and increase the risk of secondary infection to the patients. Burns affects the patient’s immune system. The ratio between pro and antiinflammatory mediators are determining the patient’s subsequent status.
Analisis Mobilisasi Sel T CD4+ dan CD8+ pada Timus Ayam Pedaging Pasca Infeksi Salmonella typhimurium dan Pemberian Simplisia Polyscias obtusa Swastika Pinca Pinca; Muhammad Sasmito Djati; Muhaimin Rifa’i
Biotropika: Journal of Tropical Biology Vol 1, No 1 (2013)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Penggunaan antibiotik dalam pakan sebagai perlindungan bagi ternak dari penyakit memiliki dampak negatif yakni adanya kandungan residu sehingga diperlukan adanya bahan substitusi antibiotik. Simplisia daun kedondong laut (Polyscias obtusa) diduga mengandung senyawa flavonoid yang diduga dapat berperan sebagai agen imunomodulator dalam sistem imun seluler. Tujuan dari penelitian ini adalah untuk mengetahui peran kandungan simplisia daun Polyscias obtusa dalam pakan ayam sebagai imunomodulator pada ayam yang telah diinfeksi bakteri Salmonella typhimurium, ditinjau dari profil ekspresi CD4+, CD8+ dan B220+ yang dianalisis melalui Flowcytometri. Tahapan penelitian meliputi uji konfirmasi bakteri S.typhimurium, persiapan kultur bakteri dan simplisia daun P. obtusa, infeksi bakteri secara oral 500 µl, pembuatan pakan konversi dengan perlakuan simplisia dosis 1 (0.08%), dosis 2 (0.16%) dan dosis 3 (0.26%), isolasi organ timus dan sel T-limfosit dan analisis flowcytometri. Hasil yang diperoleh menunjukan bahwa jumlah relatif CD4+, CD8+ dan B220+ mengalami peningkatan pada perlakuan pakan pabrik+infeksi bakteri Salmonella dan perlakuan penambahan simplisia dosis 2 (0.16%) dengan hasil yang berbeda nyata berdasarkan waktu pemberian pada jumlah relatif sel CD8+. Hal ini membuktikan bahwa simplisia daun P. obtusa memiliki kemampuan dalam memaksimalkan fungsi sistem imun.
Aktivitas Imunomodulator Ekstrak Buah Mengkudu pada Mencit yang Diinfeksi Staphylococcus aureus (IMMUNOMODULATORS ACTIVITY OF NONI (MORINDA CITRIFOLIA L.) FRUIT EXTRACT IN MICE INFECTED WITH STAPHYLOCOCCUS AUREUS) Zumrotul Mufidah; Muhaimin Rifa’i; Sri Rahayu
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.221 KB)

Abstract

This study aim was to determine the immunomodulatory activity of noni (Morinda citrifolia L.) fruitextract in mice infected with Staphylococcus aureus. Mice were divided into two group :  non-infected  andinfected. Non Infected group was without S. aureus infection whereas the infected group was infected withS. aureus. Group contain control, dose 1 (25 mg/kg BW), dose 2 (100 mg/kg BW), and dose 3 (300 mg/kg BW).Oral treatment carried out for 20 days in every morning and each sample was injected with  S. aureus atday 21 with 109 cell/mL. Relative number of T cell (CD4+, CD4+CD25+),) and cytokine interferon-ã fromCD4+ T cell (CD4+IFN-ã+) subsets was measured using the BD FACSCaliburTM Flowcytometer. Data wereanalyzed by using Analysis of Varians (p<0,05) and SPSS 16 for windows. The result showed thatadministration of noni crude extract was significantly change the relative number of CD4+, CD4+IFN-ã+,and CD4+CD25+ T cells. Treatment of noni crude extract in non-infection group could increase  relativenumber of CD4+, CD4+IFN-ã+  and CD4+CD25+ T cells that might be caused by active compounds of noni asmitogen.  Giving of noni crude extract in infected group could reduce  the relative number of CD4+, CD4+CD25+and CD4+IFN-ã+ T cells due to it  active compounds as anti-inflamation. Noni fruit extract can be used aspreventive therapy on S. aureus infection  because it contains active compounds as an anti-inflammationeffect.
Aktivitas Ekstrak Daun Kelor Terhadap Sel-T Helper dan Sel-T Sitotoksik pada Mencit yang Diinfeksi Salmonella thypi (ACTIVITY OF AQUEOUS LEAF EXTRACT OF HORSERADISH TREE ON HELPER T- CELL AND CYTOTOXIC T- CELL IN MICE INFECTED WITH SALMONELLA THYPI) Akhmad Fathir; Muhaimin Rifa’i; Widodo .
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.914 KB)

Abstract

Typhoid fever is caused by Salmonella typhi infection, and it is a still problem in many developingcountries, including Indonesia. Typhoid fever occurs due to T cells, immune system, especially CD4+ andCD8+ T cells, are deficient. This condition can cause S. thypi infects human body cells . The study aim wasto evaluate profile CD4+ and CD8+ T cells in mice spleen (Mus musculus) infected with  S. thypi afterinducted with aqueous leaf extract of horseradish tree. An experimental laboratory studiy was conductedusing completely factorial randomized design. Mice were divided into two groups, ie non infection group(induced with aqueous leaf extract of horseradish tree, at dose 0 mg/kg BW, 14 mg/kg BW, 42 mg/kg BWand 84 mg/kg BW) and infection group, the Micewere infected with S. thypi (induced with aqueous leafextract of horseradish tree, at dose 0 mg/kg BW, 14 mg/kg BW, 42 mg/kg BW and 84 mg/kg BW). The resultshowed that aqueous leaf extract of horseradish tree increased the number CD4+  of and CD8+ T cells  in allgroups of mice in conclusion administration of aqueous leaf extract of horseradish tree at high dose havecauses immunosuppressive in immune system. Aqueous leaf extract of horseradish tree have aimmunostimulatory and immunosuppressive functions in CD4+ and CD8+ T cells.
Aktivitas Ekstrak Daun Kelor terhadap Respons Imun Humoral pada Mencit yang Diinfeksi Salmonella typhi (ACTIVITY OF KELOR LEAF EXTRACT ON HUMORAL IMMUNE RESPONSE IN MICE POST SALMONELLA TYPHI INFECTION) Mohammad Hefni; Muhaimin Rifa’i; Widodo .
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.095 KB)

Abstract

The aim of this research was to analyze the activity of  kelor (Moringa oleifera Lam) leaf extract onhumoral immune response in mice infected with Salmonella typhi. Mice were divided into two groups : non-infected and infectedS. typhi groups. Each group was administered orally for 20 days with varied doses ofkelor leaf extract i.e. dose (0 mg/kg BW), dose 1 (14 mg/kg BW), dose 2 (42 mg /kg BW), and dose 3 (84 mg/kg BW).  Then all of the sample in infected groups were injected with 108 cells S. typhi. The humoralimmunity responses were determined by observing the number of lymphoid B cell (B220) and naive Thecell (CD4+CD62L+) by using software BD CellQuest Flowcytometry. The data were analysed using Two-Way ANOVA (P<0.05), with SPSS 16.0 for Windows.  The kelor leaf extract showed imunostimulatoryactivity by significantly improved the number of lymphocyte B cell (B220),  and naive Th Cell (CD4+CD62L+)in mice infected with S. typhi.  The lower doses (dose of 14 mg/kg BW, and 42 mg/kg BW) of kelor leafextracts was more effective than the highest dose (84 mg/kg BW). On the other  hand, the high dose showedimunosupresor activity on naive Teessor Th Cell.  However, immunosupressor activity on naïve Th cell wasobserved on the mice given the highest dose of extract.
Sel T Regulator CD4+CD25+ Mencegah Terjadinya Fenotip Letal pada Mencit Defisiensi CD122 Muhaimin Rifa’i; Widodo -
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.417 KB)

Abstract

Mice with a deficiency of cluster of differentiation/CD122 molecules experience increased memory Tcells. Increased memory T cells are activated and interfered with homeostasis that cause death at the ageof 10 ~ 12 weeks. To clarify whether the expression of CD25 molecules on CD4+ T cells responsible for thedevelopment of lethal phenotypes in CD122-/- mice, we did a transfusion of CD4+CD25+ T cells from normalmice to CD122-/- neonates. Transfusion of purified CD4+CD25+ T cells as much as 3 x 104 can prevent theoccurrence of lethal phenotypes generally experienced by CD122-/- mice. Transfusion of CD4+CD25+ T cellsin CD122-/- neonates cause all of the abnormalities that occur in T cell and leukocyte cells can be preventedand develop into normal. Similarly, the hematocrit that decreased dramatically in CD122-/- mice candevelop normally after receiving a transfusion of CD4+CD25+ T cells. In contras, transfusion of CD4+CD25-T cells in CD122-/- mice did not have the effect of preventing the development of the abnormalities inCD122-/- mice. CD4+CD25+ T cells that are lost in periphery of CD122-/- mice can restore to normal afterreceiving a transfusion of CD4+CD25+ T cells. These results clearly show that the expression of IL-2R?(CD25) on CD4+ T cells become pre-requisite for CD4 T cell population in order to play a role as regulatorcells.
The Control of Human Immunosystem by Using Paeony Root Drug Hideo Tsuboi; Muhaimin Rifa’i; Khaled Hossain; Izumi Nakashima; Haruhiko Suzuki
Journal of Tropical Life Science Vol. 1 No. 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Paeoniflorin (PF), isolated from paeony root, has been used as a herbal medicine for more than 1200 years in China, Korea and Japan for its anti-allergic, anti-inflamatory and immunoregulatory effects. In this study, we found that PF induces apoptosis in both murine T-lineage cells and human T-cell leukemia Jurkat cells. This apoptosis was mediated through the reduction of mitochondrial membrane potential, activation of caspase and fragmentation of DNA. Interestingly, PF induced generation of reactive oxygen species (ROS) and a reducing agent, dithiothreitol (DTT), and a ROS scavenger, N-acetyl cysteine (NAC), successfully attenuated the PF-induced apoptosis. Additionally, PF induced the phosphorylation of three mitogen-activated protein (MAP) family kinases, extracellular signal-regulated kinase, c-Jun amino-terminal kinase (JNK) and p38 MAP kinase. Curcumin, an anti-oxidant and JNK inhibitor, inhibited PF-induced apoptosis, suggesting the possible involvement of curcumin-sensitive JNK or other redox-sensitive elements in PF-induced apoptosis. These results partially explain the action mechanism of PF-containing paeony root as a herbal medicine.Keywords: paeoniflorin; paeony root; apoptosis; reactive oxygen species (ROS); redox; CD8+ CD122+ regulatory T cells
Potential Regulatory Functions of Propolis to Ameliorate Hematopoietic Disorder in Diabetic Mouse Model Muhaimin Rifa’i
Journal of Global Pharma Technology Volume 09 Issue 03
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes mellitus (DM) in autoimmune case, related to cytotoxic T cells activation as a response to autoantigen. In the recent studies, propolis is known as a potential immunomodulator. The aim of this study is to determine the effect of propolis as a regulator to ameliorate diabetic mouse model. In vivo experiment was done using streptozotocin-induced diabetic mice. There are five experiment groups; that are DM group (diabetic mice model without treatment); propolis ethanolic extract treatment group doses of 50, 100, and 200 mg/kg BW; and normal mice group. Effect of propolis was observed 14 days post treatment by flow cytometric analysis. The number of CD8+CD62L-, CD4+CD25+CD62L+(Treg), B220+,  and NK+ cells was assessed from splenic cells. The result showed that administration of propolis for 14 days could reduce the number of cytotoxic T lymphocytes (CTL) memory significantly (p ≤ 0, 05). Especially the doses of 100 and 200 mg/kg BW could suppress the activation of CTL quite similar to those normal groups. Propolis with the dose of 200 mg/kg BW increase the number of  Treg, B220, and NK cells. The increase of Treg may reduce the activation of CTL and prevent tissue damage by autoreactive cells, and finally hematopoietic imbalance can be overcome. Keywords: B220, diabetes mellitus, immunomodulatory, NK, propolis.
Effectiveness of Ethanolic Propolis Extract (EPE) Administration in Ameliorating Diabetic Mice Model Muhaimin Rifa’i
Journal of Global Pharma Technology .
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Diabetes mellitus (DM) is a group of metabolic disorder characterized by hyperglycemia resulting from defects in insulin action, insulin secretion, or both. Many efforts have been made to overcome this disease but until now has not been fully successful. This study aimed at examining CD34+, TER119+VLA4+, B220+  cell population,  and stromal cell-derived factor 1 (SDF-1) expression in an attempt to correct homeostasis indiabetic mice model with active disease.Three different EPE doses were tested (50, 100, and 200 mg/kg BW).  The oral administration of EPE was conducted for 14 days. On day 15, the bone marrow cells were isolated and then subjected to flow cytometry analysis, and data was processed by One-way ANOVA (p < 0.05) and Tukey test using SPSS 16.0 for Windows. The results showed that administration of EPE with the doses of 50 and 100 mg/kg BW) potentially increased cell proliferation. In contrast, the doses of 200 mg/kg BW seem to be in potentially suppressing cell proliferation. Taken together, propolis, which is involved in the induction of proliferation, show a rather in low doses, while high dose will give a suppression effect on cell proliferation and differentiation. Therefore, the use of propolis for treatment should consider the appropriate dose.  Keywords: B220, CD34, Diabetes mellitus, SDF-1, TER-119+VLA-4+.
Co-Authors Akhmad Fathir Aryati Haruhiko Suzuki Hideo Tsuboi Iswinarno Doso Saputro Izumi Nakashima Khaled Hossain Moch Sasmito Djati Mohammad Hefni Septin Mauludiyana Swastika Pinca Pinca Widodo - Yoes Prijatna Dachlan Yundari, Yundari Zumrotul Mufidah