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All Journal Jurnal Fitopatologi Indonesia Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL ILMIAH AGRINECA
Christanti Sumardiyono
Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, UGM Jl Flora no.1, Bulaksumur, Yogyakarta 55281
Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Materials Science & Nanotechnology Medicine & Pharmacology

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Potensi Ekstrak Lengkuas sebagai Fungisida Nabati untuk Mengendalikan Penyakit Karat Daun Anggur (Phakopsora euvitis) Ani Widiastuti; Reza Fredo Simarmata; Christanti Sumardiyono
Jurnal Fitopatologi Indonesia Vol. 16 No. 3 (2020)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.16.3.135-143

Abstract

Potency of Galangal Extract as Botanical Fungicide to Control Grape Leave Rust Disease (Phakopsora euvitis) Grape leaf rust caused by Phakopsora eutivitis is an important disease on grape plants. Botanical pesticide is important to be developed as its application may reduce the usage of synthetic chemical pesticides. This study aimed to determine potency of galangal extract as botanical fungicide against P. euvitis, compared with mancozeb. The methods were galangal extract preparation in evaporated methanol, in vitro toxicity test of galangal extract for LC50 determination, and in planta test using grape seedlings in polybags. LC50 was determined by using SAS JMP Statistical Discovery Program. In planta test was carried out by spraying urediniospore suspension with density of 1 x 106 spores.mL-1, followed by galangal extract or mancozeb spraying at LC90 concentration three days after inoculation. The result showed that galangal extract inhibited spore germination of P. euvitis. LC50 of the galangal extract was 18.33 ppm; LC90 was 53.72 ppm; while mancozeb LC50 was 65.52 ppm and LC90 was 190.71 ppm. In planta experiment showed that galangal extract of 53.72 ppm (LC90) reduced the disease intensity of leaf rust by 16% on the 18th day, while mancozeb of 190.71 ppm (LC90) reduced the disease intensity by 26.4% compared to positive control or untreated-inoculated plants. This study showed that galangal extract is potential to be developed as botanical fungicide to control grape leaf rust disease
Karakterisasi Isolat Rhizoctonia sp. Patogenik dan Rhizoctonia Mikoriza Pada Tanaman Anggrek Tanah Spathoglottis plicata Soelistijono Soelistijono; Achmadi Priyatmojo; Endang Semiarti; Christanti Sumardiyono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v16i2.121

Abstract

Rhizoctonia mikoriza merupakan jamur yang mampu berasosiasi dengan anggrek tanah. Selain sebagai mikoriza, terdapat isolat Rhizoctonia sp. patogen dan penyebab penyakit busuk akar pada Spathoglottis plicata. Penelitian ini bertujuan mengetahui perbedaan antara Rhizoctonia sp. patogen dan Rhizoctonia mikoriza secara morfologi dan molekular menggunakan teknik RAPD. Hasil penelitian secara morfologi menunjukkan bahwa warna koloni, panjang dan jumlah inti sel isolat Rhizoctonia sp. patogen dan Rhizoctonia mikoriza pada S. plicata tidak berbeda, tetapi berbeda pada ketebalan sel dan pengelompokan isolat berdasarkan uji anastomosis hifa. Teknik molekuler RAPD menunjukkan bahwa setiap isolat Rhizoctonia sp. patogen dan Rhizoctonia mikoriza memiliki perbedaan pada struktur DNA.
Dominansi dan Seleksi Jamur Aspergillus Perusak Gaplek H. A. Oramahi; Christanti Sumardiyono; Nursamsi Pusposendjojo; Haryadi Haryadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i1.2646

Abstract

Dried cassava is a suitable host for the growth and development of the genus Aspergillus. Fungi reported to grow on dried cassava were A. clavatus, A. flavus, A. foetidus, A. fumigatus, A. niger, A. ochraceus, A. oryzae, A. tamarii, A. zonatus, dan Aspergillus sp. From those species, A. flavus is the most important species because of its toxigenic characteristic on agricultural product. This study was conducted to study dominant species of Aspergillus causing the most severe deterioration on stored dried cassava and causing the highest deterioration in dried cassava. The isolates were then cultured for determination of dominant species. The in vitro experiment was to obtain the species of Aspergillus that is resulted in highest change of dried cassava spoilage. Based on relative index frequency (Rif) and presence index of the fungus (Pif), A. flavus was the most dominant species and causing the highest deterioration on the dried cassava.
Pengaruh Lengas Nisbi dan Suhu terhadap Kerusakan Gaplek Akibat Aspergillus flavus Selama Penyimpanan H. A. Oramahi; Christanti Sumardiyono; Nursamsi Pusposendjojo; Haryadi Haryadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2723

Abstract

A study on the spoilage of dried cassava by Aspergillus flavus was still limited so that such study is very important to do. This experiment was done to examine the effect of relative humidity (RH) and storage room temperature on the development of A. flavus and dried cassava spoilage during storage time. Based on the population of A. flavus, starch content, reduction sugar content, and water content of dried cassava, it was concluded that RH 65%, the temperature of 30 and 35oC had better storage condition for dried cassava because it resulted in the lowest population of A. flavus and the lowest deterioration of dried cassava.
KARAKTERISTIK ISOLAT Rhizoctonia sp. PATOGENIK DAN Rhizoctonia MIKORIZA PADA TANAMAN ANGGREK TANAH (Spathoglottis plicata) S. Soelistijono; Christanti Sumardiyono; Achmadi Priyatmojo; Endang Semiarti
JURNAL ILMIAH AGRINECA Vol. 12 No. 1 (2012): JURNAL AGRINECA
Publisher : Fakultas Pertanian, Universitas Tunas Pembangunan Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36728/afp.v12i1.497

Abstract

Mycorrhizal Rhizoctonia is a fungus that capable to associate with terrestrial orchids. Apart from being mycorrhizal, there are isolates of Rhizoctonia sp. that are pathogenic and caused root rot disease on Spathoglottis plicata. This study aimed to know the differences between pathogenic Rhizoctonia sp. and mycorrhizal Rhizoctonia in morphology and molecular structure using RAPD technique. The results showed that colony colour,  cell lenght and nucleus number a several isolates of pathogenic Rhizoctonia sp. and of mycorrhizal Rhizoctonia on S. plicata had no differences, but had differences on cell thickness and isolate grouping based on hyphal anastomosis test. RAPD molecular technique showed that each isolate of pathogenic Rhizoctonia sp. and mycorrhizal Rhizoctonia had differences on DNA structure.
Co-Authors Achmadi Priyatmojo Ani Widiastuti Endang Semiarti Haryadi Haryadi Hasan Ashari Oramahi Nursamsi Pusposendjojo R Soelistijono Reza Fredo Simarmata