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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)
Alfarisa Nururrozi
Gadjah Mada University
Veterinary Other

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Journal : Jurnal Veteriner

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KAJIAN FILOGENETIK ULAR SANCA BATIK (Malayopython reticulatus) LOKALITAS JAWA BERDASAR SEKUEN GEN SITOKROM B Slamet Raharjo; Sri Hartati; Soedarmanto Indarjulianto; Rini Widayanti; Alfarisa Nururrozi
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.009 KB) | DOI: 10.19087/jveteriner.2018.19.2.284

Abstract

Reticulated python (Malayopython reticulatus) Javan locality has different phenotype among West Java, Central Java and East Java locality. This research aim was to study the phylogenetic of reticulated python (M. reticulatus) Java locality based on the partial gene cytochrome b sequences. Fifteen males and females adult healthy reticulated pythons (5 from West Java, 5 from Central Java and 5 from East Java) were used in this study. As much as 1 mL blood samples collected from ventral coccygeal vein, put in Eppendorf with EDTA then sent to Laboratory of Biochemistry, Faculty of Veterinary Medicine, Gadjah Mada University for total Deoxyrybo Nucleic Acid (DNA) extraction and DNA amplification with Polymerase Chain Reaction (PCR) method. DNA sequencing was done in First Base Company Singapore by PT. Genetika Science Jakarta. The gene cytochrome b sequences were phylogenetically analysed using Neighbor joining (MEGA v. 5.0) method. The length of partial gene cytochrome b nucleotides sequence of M. reticulatus from West Java, Central Java and East Java locality was 819 bp. The phylogram using Neighbor joining method showed that Malayopython reticulatus Central Java locality was differentiated from West Java locality, while the East Java locality was undifferentiated from West Java and Central Java locality, namely in the same group. It could be concluded that reticulated python (M. reticulatus) Java locality has partial gene cytochrome b sequences with 819 bp. The phylogenetic of Malayopython reticulatus West Java and several from East Java were located at same branch with M. reticulatus GenBank, while the Central Java and several from East Java were located at different branch. It needs further research with more samples and validity of localities for better result.
Studi Retrospektif Profil Hemogram Kasus Peritonitis Menular Tipe Efusif pada Kucing Alfarisa Nururrozi; Agistanya Andimi; Yanuartono Yanuartono; Soedarmanto Indarjulianto
Jurnal Veteriner Vol 23 No 1 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.642 KB) | DOI: 10.19087/jveteriner.2022.23.1.112

Abstract

Feline Infectious Peritonitis (FIP) merupakan penyakit pada kucing dengan tingkat mortalitas tinggi,sehingga membutuhkan diagnosa yang cepat untuk kepentingan prognosis. Penelitian ini bertujuan mengevaluasi profil hemogram kucing yang terinfeksi FIP tipe efusif. Dua puluh ekor kucing yang telah terdiagnosa FIP di Klinik Hewan Departemen Ilmu Penyakit Dalam, Fakultas Kedokteran Hewan Universitas Gadjah Mada digunakan dalam penelitian. Diagnosa FIP ditegakkan berdasarkan pemeriksaan klinis, ultrasonografi, rontgen, uji rivalta, dan uji rapid test. Profil hemogram yang dianalisis meliputi gambaran hematologi rutin dan kimia darah. Profil hemogram pada kucing terinfeksi FIP tipe effusif, diketahui mengalami penurunan hematokrit, hiperproteinemia, dan leukositosis dengan rerata masing-masing 22,9±7,4%; 9,0±2,2 g/dL; 22425±4116 sel/mm3. Gambaran eritrosit, hemoglobin dan fibrinogen masih dalam kisaran normal. Sebanyak 90% kucing terinfeksi FIP efusif mengalami neutrofilia dan 75% kucing mengalami limfopenia dengan rerata masing-masing 20066±3337 sel/mm3 dan 1861±1818 sel/mm3. Profil hemogram kimia darah diketahui 60% kucing mengalami kenaikan SGPT dan SGOT dengan rerata 138,4±72,3 IU/L dan 101±60,5 IU/L. Sebanyak 90% kucing mengalami hiperglobulinemia dengan rerata 6,7±0,8 g/dL dan semua kucing memiliki rasio albumin:globulin yang rendah dengan rerata 0,3±0,1. Kucing terdiagnosa FIP efusif memiliki gambaran hemogram leukositosis, neutrofilia, limfopenia, hiperglobulinemia, dan penurunan rasio albumin-globulin.
Co-Authors Agistanya Andimi Jeffi Chandra Ajiguna Mulya Fitranda Rini Widayanti Slamet Raharjo Soedarmanto Indarjulianto Sri Hartati Yanuartono Yanuartono Yanuartono, Yanuartono