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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner
Khrisdiana Putri
Departemen Kesmavet Fakultas Kedokteran Hewan Universitas Gadjah Mada
Veterinary

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Detection of Antibiotic Residues in Chicken Meat and Eggs from Traditional Markets at Yogyakarta City Using Bioassay Method Dyah Ayu Widiasih; Yatri Drastini; Doddi Yudhabuntara; F. Lintang R. Daru Maya; Prisha Lini Sivalingham; Heru Susetya; Widagdo Sri Nugroho Sri Nugroho; M. Th. Khrisdiana Putri; Roza Azizah Primatika; Bambang Sumiarto
Acta VETERINARIA Indonesiana 2019: Special Issues
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.823 KB) | DOI: 10.29244/avi.0.0.1-6

Abstract

Studies on antibiotic residues content in food of animal origin are currently needed to support veterinary public health programs. The present study was described bioassay method for the detection of antibiotic residues in chicken meat and eggs from traditional market at Yogyakarta City. A number of twenty-four chicken meat samples and 24 egg samples were taken from 8 traditional markets in Yogyakarta city. Samples were examined at Centre for Veterinary Wates, Yogyakarta, Indonesia using bioassay method for screening detection of penicillin, aminoglycoside, macrolide and tetracycline residues. This bioassay method using some bacteria, such as Bacillus stearothermophillus, B. cereus, B. subtilis, and Kocuria rizophila. A percentage of the results showed that 8.33% (2/24) samples of chickens tested positively contained the oxytetracycline antibiotic residues. Meanwhile, as much as 75% (18/24) samples of positive eggs contain penicillin antibiotic residues, positive residues of aminoglycoside amounted to 12.5% (3/24) and the positive residues of oxytetracycline also amounted to 12.5% (3/24).
Gonadotropine Releasing Hormone (GnRH) Receptor Profile from Cow’s Hypothalamus Irma Dian Nurani; Claude Mona Airin; Pudji Astuti; Khrisdiana Putri; Bambang Sutrisno
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.51882

Abstract

Gonadotropine Releasing Hormone (GnRH) is one of the recommended hormones to overcome ovulation problems and it can increase pregnancy rate so that it is used in government programs to increase cattle population in Indonesia, although the results are not yet optimal. The purpose of this study is to compare the composition of bovine GnRH receptor nucleotides with the GenBank reference so that the level of genetic diversity of bovine receptors in Indonesia is known. PCR product sequencing using Promoter F and Exon 1 R primers were further aligned with the reference sequences of Bos Taurus GnRHR mRNA GenBank using the MEGA X program. The results of the analysis found the presence of Single Nucleotide Polymorphism (SNP) in the coding region  of 1st cow  position 38(A> T), 261(C > T), 342(C >T), 411(C >T) and 495(C > T) and 2nd cow positions 261(C >T). Changes in amino acids were also detected in 1st cow position 13 (H> L). The presence of SNP was found to indicate genomic variation between individuals at cattle receptors in Indonesia.
Co-Authors Bambang Sumiarto Bambang Sutrisno Claude Mona Airin Doddi Yudhabuntara Dyah Ayu Widiasih F. Lintang R. Daru Maya Heru Susetya Irma Dian Nurani Prisha Lini Sivalingham Pudji Astuti Roza Azizah Primatika Widagdo Sri Nugroho Sri Nugroho Yatri Drastini