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All Journal AGRIVITA, Journal of Agricultural Science agriTECH Jurnal Riset Industri Hasil Hutan Jurnal Kimia Mulawarman The Indonesian Biomedical Journal Jurnal Ilmiah Manuntung Journal of Tropical Pharmacy and Chemistry JURNAL ILMU KEFARMASIAN INDONESIA Jurnal Ilmiah Inovasi ULIN: Jurnal Hutan Tropis Jurnal Riset Teknologi Industri Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society Bivalen: Chemical Studies Journal
Enos Tangke Arung, Enos Tangke
Faculty Of Forestry, Mulawarman University Samarinda
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Other

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Journal : The Indonesian Biomedical Journal

 1 
Brucea javanica Leaf Extract Induced Apoptosis in Human Oral Squamous Cell Carcinoma (HSC2) Cells by Attenuation of Mitochondrial Membrane Permeability Britanto Dani Wicaksono; Enos Tangkearung; Ferry Sandra
The Indonesian Biomedical Journal Vol 7, No 2 (2015)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v7i2.76

Abstract

BACKGROUND: Brucea javanica extract has been reported to have anti-proliferative and cell death induction activities. B. javanica extract was reported to induce apoptosis through caspase cascade. Most of investigated B. javanica extracts were derived from seeds and fruits, or commercially available oil emulsion. Therefore we conducted a study on B. javanica leaf extract (BJLE) in oral cancer cells.METHODS: B. javanica leaves were collected, identified, minced, dried, extracted with distilled ethanol at room temperature for 24 hours, filtered and evaporated. Resulted BJLE was stored at 4°C. Human oral squamous cell carcinoma (HSC)-2 cells were fasted for 12 hours and treated with BJLE in various concentrations for 24 hours. Cells were then quantified with 3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide (MTT) assay, demonstrated with 4',6'-diamidino-2-phenylindole (DAPI) staining. To find out mitochondrial membrane permeability (MMP), mitochondrial membrane potential (ΔΨM) was analyzed.RESULTS: BJLE reduced percentage of viable HSC-2 cells in a concentration dependent manner. BJLE induced apoptosis in HSC-2 cells. With treatment of 50 μg/ml BJLE, fragmented nuclei were seen. ΔΨM of HSC-2 cells treated with 50 μg/ml BJLE were shifted to the left, meaning that BJLE induced reduction of ΔΨM and attenuation of MMP.CONCLUSION: Our results suggested that BJLE could induce apoptosis by attenuating MMP.KEYWORDS: Brucea javanica, leaf, apoptosis, HSC-2, MTT, DAPI, mitochondria, permeability
Co-Authors A.A. Ketut Agung Cahyawan W Abdul Rasyid Zarta Abdul Rasyid Zarta Amalia Riska Setyani Bayu Susetya Ananda Britanto Dani Wicaksono Dyah Ayu Dewi Listyaningrum Edy Budiarso Edy Budiarso Edy Budiarso Enih Rosamah Ernita Obeth Ernita Obeth Eva Nurmarini Farida Ariani Farida Aryani Ferry Sandra Hamka Nurkaya Heriad Daud Salusu Heriad Daud Salusu Heriad Daud Salusu Irawan Wijaya Kusuma, Irawan Wijaya Khairunnisa, Binti Kuniyoshi Shimizu KUNIYOSHI SHIMIZU Kusuma, Irawan W Mark Rayment Nataniel Tandirogang Nor Hikmah Rico Ramadhan Ritson Purba Ritson Purba, Ritson Rosamah, Enih Rosamah, Enih Rudianto Amirta Ryuichiro Kondo Saat Egra Sarah Azzara Dikarulin Sari, Ayu Mitha Sukemi Sukemi Suwinarti, Wiwin Tasya Ananda Putri Teddy Tranoto Teddy Tranoto, Teddy Wijaya Kusuma, Irawan Wiwin Suwinarti Yanti Puspita Sari Yuliansyah Yuliansyah Yundari, Yundari