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Phylogenetic relationship of Gram Negative Bacteria of Enterobacteriaceae Family in the Positive Widal Blood Cultures based on 16S rRNA Gene Sequences Darmawati, Sri; Sembiring, Langkah; Asmara, Widya; Artama, Wayan T.; Kawaichi, Masashi
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Abstract

The purpose of this study was to analyze the phylogenetic relationship of Gram negative bacteria (3strains of Salmonella typhi, 1 strain of Escherichia coli, 1 strain of Serratia marcescens, and 3 strains of Enterobactercloacae) of Enterobacteriaceae family in positive Widal blood cultures based on 16S rRNA gene sequences. Theresults respectively showed that each two 16S rRNA gene clones of Serratia marcescens KD 08.4 had a closerelationship with 16S rRNA gene of Serrratia marcescens ATCC 13880 (similarity: 99.53-99.8%), Eschericia coliBA 30.1 with Eschericia coli ATCC 11775T (similarity: 99.38-99.67%), Salmonella typhi BA 07.4, Salmonella typhiKD 30.4, and Salmonella typhi SA 02.2 with Salmonella typhi ATCC 19430T (similarity: 99.4-100%) as well as theisolates of Enterobacter cloacae SA 02.1, Enterobacter cloacae BA 45.4.1, one 16S rRNA gene clone of Enterobactercloacae TG 03.5 with Enterobacter cloacae ATCC 23373 (similarity: 99.0-99.87%).
Chemosystematic of Enterobacteriaceae Familia Obtained from Blood Cultures Based on Total Protein Profiles Darmawati, Sri; Sembiring, Langkah; Asmara, Widya; Artama, Wayan T.; Anwar, Syaiful
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

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Abstract

The purpose of this study was to determine the chemosystematic of 14 strains of bacteria in blood cultures from Semarang using 1 reference strain S. typhi NCTC 786, based on the total protein profi les with the similarity relationship analysis based on Simple Matching Coeffi cient (SSM) analysis and algorithm methodof unweighted pair group with averages (UPGMA) presented in a dendrogram. The results showed that thechemosystematic based on the total protein profi les using SDS-PAGE method can classify the member ofbacterial strains of each species. The Clusters respectively consist of 4 strains of S. typhi (similarity: 89.7%),2 strains of Ser. marcescens (similarity: 89.7%), two strains of E. coli, and one strain of Salmonella ssp, S. typhi NCTC 786 (similarity: 100%). Those three incorporated clusters had the similarity value of 75.3%. Those four strains of Ent. cloacae composed in one cluster (similarity: 100%) are incorporated in a cluster consisting of one strain of Kleb. pneumoniae (similarity: 92.9%). Both clusters were incorporated in a cluster consisting of S. typhi NCTC 786 (similarity: 67.9%).Key words: Enterobacteriaceae, chemosystematic, blood cultures, protein profile
Profil Protein Lima Jenis Daging yang Direndam Daun Pepaya Berbasis SDS-PAGE Susanti, A.Meryam; Darmawati, Sri; Maharani, Endang Tri Wahyuni
Gorontalo Journal of Public Health VOLUME 2 NOMOR 1, APRIL 2019
Publisher : Universitas Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32662/gjph.v2i1.482

Abstract

Meat is an important food for fulfill nutritional needs, many of meats are consumed as a source of highest quality nutrition for humans, especially as a source of protein. Papaya leaves contain the enzyme papain (a protase enzyme that can hydrolyze proteins), so that it can be used to soften meat. The purpose of this study was to look at an overview of protein profiles in five types of meat, namely goat, beef, buffalo, free-range chicken and broiler chicken which were soaked in papaya leaves. The protein profile of five types of meat was analyzed using the SDS-PAGE 12% method. The results showed that the control meat of goat, beef, buffalo, free-range chicken and broiler chicken which were not soaked in papaya leaves showed that there were many major protein bands compared to minor protein bands. Whereas in goat, beef, buffalo, free-range chicken and broiler chicken which have been soaked in papaya leaves, the results were different compared to the control, there were many minor protein bands. While the major bands only have 6 to 9 protein bands. Based on these results indicate that immersion with the enzyme papain contained in papaya leaves can break down peptide bonds, if it works on meat it can be broken down so the meat becomes tender and protein bands in the form of micromolecules.Daging merupakan bahan pangan yang penting dalam memenuhi kebutuhan gizi, banyak dikomsumsi sebagai sumber nutrisi yang berkualitas bagi manusia terutama sebagai sumber protein. Daun pepaya mengandung enzim papain (enzim protase yang dapat menghidrolisa protein), sehingga dapat digunakan untuk melunakkan daging. Tujuan penelitian ini untuk melihat gambaran profil protein pada lima jenis daging yaitu daging kambing, sapi, kerbau, ayam kampung dan ayam potong yang direndam daun pepaya. Profil protein lima macam daging dianalisis menggunakan metode SDS-PAGE 12%. Hasil penelitian menunjukkan pada daging kontrol yaitu daging kambing, sapi, kerbau, ayam kampung dan ayam potong yang tidak direndam daun pepaya menunjukkan terdapat banyak pita protein mayor dibandingkan pita protein minor. Sedangkan pada daging kambing, sapi, kerbau, ayam kampung dan ayam potong yang telah direndam daun pepaya menunjukkan hasil yang berbeda dibandingkan dengan kontrol yaitu pada semua daging terdapat banyak pita protein minor. Sedangkan pita mayor hanya terdapat 6 sampai 9 pita protein saja. Berdasarkan hasil tersebut menunjukkan bahwa perendaman dengan enzim papain yang terdapat dalam daun pepaya dapat memecah ikatan peptida, jika bekerja pada daging dapat diuraikan sehingga daging menjadi empuk, dan pita protein berbentuk mikromolekul.
Analisis Profil Protein Daging Kerbau dengan Variasi Konsentrasi Garam serta Pengasapan Berbasis SDS-Page La’lang, Marselaonety; Darmawati, Sri; Kartika, Aprilia Indra
Jurnal Labora Medika Vol 2, No 2 (2018): Jurnal Labora Medika
Publisher : Jurnal Labora Medika

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Abstract

Daging kerbau memiliki nilai gizi protein dan susunan asam amino lengkap. Kandungan air dan protein yang tinggi menyebabkan daging mudah busuk, sehingga perlu dilakukan pengawetan penggaraman serta pengasapan. Protein daging kerbau dapat dipengaruhi oleh pengolahan bahan pangan, seperti kadar pemberian garam dan pengasapan. Tujuan penelitian untuk menganalisis profil protein daging kerbau dengan variasi konsentrasi garam  10%b/b, 20% b/b, 30% b/b, 40% b/b penggaraman 3 jam serta pengasapan 2 jam. Profil protein daging kerbau dapat dianalisis menggunakan metode SDS – PAGE 12%. Hasil penelitian dari profil protein daging (kontrol), penggaraman 3 jam konsentrasi garam 10%, 20%, 30% dan 40% berturut – turut 28 sub unit protein, 26 sub unit protein,  25 sub unit protein, 23 sub unit protein, dan 21 sub unit protein, sedangkan daging yang diasapkan tanpa garam, daging penggaraman 3 jam serta pengasapan 2 jam konsentrasi garam 10%, 20%, 30% dan 40% berturut -turut 22 sub unit protein, 24 sub unit protein, 20 sub unit protein, 13 sub unit protein dan 12 sub unit protein. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa semakin tinggi konsentrasi garam maka kandungan protein yang terdapat dalam daging kerbau akan rusak dan semakin sedikit hal ini menyebabkan denaturasi protein yang ditandai dengan berkurangnya sub unit protein. Namun protein daging kerbau lebih banyak rusak apabila diasapkan.
KEANEKARAGAMAN DAN POLA RESISTENSI BAKTERI PADA PASIEN YANG TERDIAGNOSA SEPSIS Batara, Murni; Darmawati, Sri; Prastiyanto, Muhammad Evy
Jurnal Labora Medika Vol 2, No 2 (2018): Jurnal Labora Medika
Publisher : Jurnal Labora Medika

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Abstract

Sepsis atau Systemic Inflammatory Response Syndrome (SIRS) adalah kondisi klinis akut dan serius yang muncul akibat adanya infeksi bakteri di dalam darah. Penelitian ini bertujuan untuk mengetahui keanekaragaman bakteri dan pola resistensi terhadap antibiotik pada sampel darah pasien yang terdiagnosa sepsis  di Laboratorium Klinik Swasta di Semarang pada bulan Mei-Juni tahun 2018. Metode penelitian ini yaitu deskriptif. Hasil penelitian didapatkan sebanyak 42 sampel darah yang ditanam pada media Bact Alert, 12 sampel dinyatakan positif tumbuh. Dari ke 12 sampel tersebut didapatkan 6 jenis bakteri penyebab sepsis yaitu Staphylococcus haemolitycus (25%), Staphylococcus aureus (16,66), Staphylococcus hominis (16,66%), Staphylococcus epidermidis (16,66%), Staphylococcus saprophyticus (8,33%),  Pantoea sp (8,33%), dan Bordetella hinzii (8,33%). Bakteri gram positif resisten terhadap antibiotik Benzylpenicillin, Oxacillin, Penicyline, dan antibiotik betalaktam lainnya. Sedangkan bakteri Pantoea sp resisten terhadap antibiotik Pipericillin/Tazobactam dan Bordetella hinzii resisten terhadap antibiotik Pipericillin/Tazobactam dan Nitrofurantoin
PENENTUAN GOLONGAN DARAH SISTEM ABO DENGAN SERUM DAN REAGEN ANTI-SERA METODE SLIDE Darmawati, Sri
Gaster : Jurnal Kesehatan Vol 17, No 1 (2019): FEBRUARI
Publisher : P3M STIKES Aisyiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.796 KB) | DOI: 10.30787/gaster.v17i1.330

Abstract

Golongan darah ABO pada manusia ditentukan berdasarkan jenis antigen dan antibodi yang terkandung dalam darahnya. Pemeriksaan golongan darah untuk mendeteksi keberadaan antigen di permukaan membran sel darah merah dengan cara mereaksikan darah manusia dengan anti A dan anti B. Penggunaan serum untuk pemeriksaan golongan darah jarang dilakukan. Tujuan penelitian ini untuk menganalisis perbedaan hasil pemeriksaan golongan darah sistem ABO menggunakan serum dan reagen dengan metode slide. Bahan yang digunakan adalah darah manusia golongan A,B,AB dan O serta reagen anti A, anti B dan anti AB. Pemeriksaan golongan darah dengan metode slide,  penilaian menggunakan skoring ( likert scale). Hasil penelitian menunjukkan grade aglutinasi yang dihasilkan oleh serum berbeda dengan grade aglutinasi menggunakan  reagen anti-sera, karena di dalam serum tidak hanya berisi antibodi tetapi ada komponen yang lainnya yang mempengaruhi reaksi aglutinasi antara antigen pada permukaan eritrosit dengan serum anti A, anti B maupun anti AB. Pemeriksaan Golongan darah manusia hasilnya lebih baik menggunakan reagen antisera
KEANEKARAGAMAN SPESIES BAKTERI PADA KULTUR DARAH WIDAL POSITIF ASAL KOTA SEMARANG BERDASARKAN KARAKTER FENOTIPIK Darmawati, Sri; Sembiring, Langkah; Asmara, Widya; T. Artama, Wayan
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Tujuan penelitian ini untuk menentukan keanekaragaman spesies bakteri pada kultur darah Widal positif  Asal kota Semarang berdasrkan karakter fenotipik. Sampel darah yang dikultur sebanyak 136 sampel berasal dari pasien rawat inap dan rawat jalan di 4 rumah sakit serta 2 puskesmas di kota Semarang (RSUD Kota Semarang, RSUD Tugurejo, RS. Islam Sultan Agung,  dan 2 Puskesmas yaitu Kedungmundu dan Bangetayu.  Kultur darah digunakan medium BacT/Alert FAN blood culture bottles (Biomerieux), subkultur digunakan medium Blood Agar Plate (BAP, OXOID) dan Mac Conkey (MC, OXOID), dilanjutkan  uji biokimia digunakan medium API 20E dan API 50CHB/E untuk identifikasi strain anggota familia Enterobacteriaceae serta APIStap (Biomerieux) untuk identifikasi spesies anggota Staphylococcus. Kultur darah positif sebanyak 59 sampel (43.4%) terdiri dari 44 sampel (32,4%) positif Staphylococcus sp. (S. aureus, S. saprophyticus, S. xylosus, S. warnei, S. hominis, S. cohnii) dan 15 sampel (11%) positif bakteri batang gram negatif anggota familia Enterobacteriaceae yaitu Enterobacter cloacae, S. typhi, Serratia marcescens, Escherichia coli, Salmonella ssp., Klebsiella pneumoniae ssp. Ozanae. Berdasarkan karakter fenotipik  bakteri batang gram negatif dapat dikelompokkan menjadi 4 kluster, kluster pertama beranggotakan S. typhi , kluster kedua beranggotakan E. coli dan Salmonella ssp., kluster ketiga beranggotakan Ser. Marcescens dan kluster keempat beranggotakan Enterobacter cloacae dan Kleb. pneumoniae ssp. Ozaenae. Bakteri kokus gram positif berdasarkan karakter fenotipiknya dapat dikelompokkan menjadi 6 kluster  yang tampak sangat bervariasi   Kata kunci:  Widal, Kultur darah, BacT/Alert FAN, API 20E, API 50 CHB/E, API Stap
Antibacterial Activity of Various Extracts of Averrhoa bilimbi against Multidrug Resistant Bacteria Prastiyanto, Muhammad Evy; Wardoyo, Fandhi Adi; Wilson, Wildiani; Darmawati, Sri
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.23600

Abstract

The multi-drug resistance (MDR) bacteria is a global health problem that causes high mortality every year. Therefore, novel antibacterial agents are needed from natural biological sources. This research aimed to investigate the antibacterial activities of various crude extracts of Averrhoa bilimbi against MDR bacteria. The antibacterial activity was calculated based on the use agar well diffusion assay and the minimum bactericidal concentration (MBC) using Mueller–Hinton broth in a microdilution method. Bacteria from wells were subcultured using inoculating loop onto a 5% sheep BAP. The best antibacterial activity, calculated as the most widely inhibitory zone and the smallest MBC values. The ethanolic extract showed antibacterial activity against the all MDR bacterial test in the agar well diffusion assay (10-14.5 mm inhibition diameter). The MBC of water extract against ESβL + CR Pseudomonas aeruginosa showed the best antibacterial activity (12.5 mg/mL). The fruit of bilimbi was shown to be potentially developed as antibacterial agents, especially for MDR strains. Further in vivo research and discovery of action mode are needed to shed light on their antibacterial effects. This study can provide new information about the benefits of bilimbi as a source of natural antibacterial againts MDR-bacteria
PENINGKATAN KETERAMPILAN BERBICARA DALAM PEMBELAJARAN BAHASA INDONESIA DENGAN DISKUSI KELOMPOK KELAS V Darmawati, Sri; ., Rosnita; ., Rustiyarso
Jurnal Pendidikan dan Pembelajaran Khatulistiwa Vol 2, No 1 (2013): Januari 2013
Publisher : Jurnal Pendidikan dan Pembelajaran Khatulistiwa

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Abstract

Peningkatan Keterampilan Berbicara dalam Pembelajaran Bahasa Indonesia dengan Diskusi Kelompok Kelas V bertujuan untuk perbaikan pembelajaran bahasa Indonesia dalam meningkatkan keterampilan berbicara dengan menggunakan metode diskusi kelompok di kelas V Sekolah Dasar Negeri 12 Sembatu Kabupaten Landak. Metode yang digunakan dalam penelitian ini adalah metode deskriptif. Bentuk penelitian deskriftif yang digunakan adalah penelitian survey. Jenis penelitian ini adalah Penelitian Tindakan Kelas. Rerata aktivitas Pra-Tindakan adalah 33,4 dan rerata setelah siklus III adalah 71,4. Maka dapat disimpulkan terdapat peningkatan keterampilan berbicara dalam pembelajaran bahasa indonesia dengan metode diskusi kelompok pada siswa kelas V SDN 12 Sembatu. Abstract : Speaking at the Learning Skills Improvement Indonesian with Class V Group Discussion SDN 12 Sembatu aims to improve the learning of Indonesian in improving speaking skills by using group discussion in class V 12 Sembatu Elementary School, Landak Regency. The method used in this research is descriptive method. The form used is descriptive research survey research. This research was classroom action research. The mean pre-action activities was 33.4 and the mean after the third cycle was 71.4. So we can conclude there is a growing skills in learning to speak Indonesian with the method of group discussion in class V SDN 12 Sembatu. Kata Kunci : Keterampilan Berbicara, Penelitian Tindakan Kelas, pada Mata Pelajaran Bahasa Indonesia
IDENTIFIKASI KONTAMINASI Salmonella Typhi BERDASARKAN AMPLIFIKASI GEN fliC PADA CINCAU DI PASAR TRADISIONAL DAN MINIMARKET KOTA SEMARANG Ernanto, Aditya Rahman; Palimbongan, Junita Rensa; Manufandu, Anjar Richardo; Darmawati, Sri
Jurnal Teknologi Laboratorium Vol 9 No 2 (2020): (In Progress)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i2.207

Abstract

Grass-jelly is one of the most popular plants consumed by people in various forms of beverage mix because it is easy to gets in traditional markets. Microorganism contamination can occur in grass-jelly due to several factors such as water for processing, equipments, processing facilities and from people who did the process. Contamination can cause various diseases, one of those is typhoid fever by Salmonella typhi. The purpose of this study was to detect S. typhi in grass-jelly based on molecular detection by amplification of the fliC gene using PCR. Steps of the methods were DNA extraction from grass-jelly samples, genomic DNA quality testing, amplification of fliC gene and agarose electrophoresis. Validation was done by culture methods on SSA media and biochemical testing. The fliC gene amplification results in grass-jelly samples (A1, A2, B1, B2, C1 and C3) showed the DNA fragments size about 1500 bp. Colony and biochemical characters isolates Peterongan were lead to S. typhi, whereas another isolate were another Salmonella spp. The conclusion of this study was that grass-jelly samples from Peterongan market in Semarang were positively contaminated by S. typhi and isolate from Pedurungan and minimarket were another Salmonella spp. Molecular-based food testing is fast enough and accurate for detecting types of bacterial contaminants but amplification of fliC gene cannot specific for S. typhi.