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All Journal Jurnal Fitopatologi Indonesia METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS
NI NYOMAN DARSINI
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology

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Keanekaragaman Jenis Tumbuhan Bahan Ramuan “Boreh Basanbuat” Untuk Memperlancar Produksi Air Susu Ibu (ASI) di Bali Enik Kriswiyanti; Ni Nyoman Darsini; Junita Hardini; Ni Putu Ariwathi
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p15

Abstract

Boreh (bahasa Bali) merupakan salah satu bentuk ramuan tradisional berupa campuran rempah-rempah yang dihaluskan, ditambah cairan (air/arak/minyak kelapa) kemudian dilulurkan pada bagian tubuh. Salah satu boreh yang dapat digunakan untuk memperlancar produksi air susu ibu (ASI) adalah boreh basanbuat. Penelitian ini bertujuan untuk mengetahui jenis dan bagian tumbuhan yang dapat digunakan untuk membuat boreh basanbuat di Bali. Metode penelitian yang digunakan: purposive sampling, pengumpulan data dengan cara wawancara, observasi dan dokumentasi dengan pedagang crakenan, pengobat tradisional (battra) dan masyarakat dari kota dan kabupaten wilayah propinsi Bali, sebanyak 90 responden. Hasil penelitian didapatkan 54 jenis tumbuhan (52 genus, 29 familia) untuk membuat 64 jenis ramuan, jenis tumbuhan yang paling banyak digunakan dari anggota famili Zingiberaceae, bagian tumbuhan yang paling banyak digunakan adalah daun (23,21%). Ramuan boreh basanbuat umumnya menggunakan 5-6 jenis tumbuhan, yaitu menggunakan: beras (Oryza sativa L.), biji kelabet (Trigonella feonum-graecum L.), akar wangi (Androphogon zizanioides (L) Urban), kayu/bubuk cendana (Santalum album L.), daun delem/ nilam (Pogostemon cablin (Blanco) Benth.) dan rimpang kencur (Kaemferia galanga L). Manfaat bahan boreh selain memperlancar produksi ASI, juga untuk aroma terapi, antiinflamasi, anti pembengkakan, antibakteri, antijamur, dan lain sebagainya.
Formulasi Sediaan Masker Gel Peel-off Ekstrak Daun Kelor (Moringa oleifera Lam.) dan Minyak Atsiri Serai Wangi (Cymbopogon nardus L. Rendle) Erlina Adhayanti; Ni Luh Arpiwi; Ni Nyoman Darsini
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p10

Abstract

Peel-off gel masks are practical masks, because it can be removed without rinsing with water. Peel-off gel mask useful for clean and tighten facial skin. Moringa leaves and citronella essential oil can be used as masks cause have antioxidant and antibacterial activities that act as anti-aging and anti-acne. The purpose of this study was to determine the combination of citronella essential oil and concentration of moringa leaf extract that produced the best mask. Essential oil was extracted by steam distillation, moringa leaves were extracted by maceration using 96% ethanol. Mask was formulated with 0, 1, 2, and 3% moringa leaf extract. The yield of citronella leaf essential oil was 0.36%±0.07 % w/w. The organoleptic test results showed that all mask formulas were stable during storage. The results of the physical properties test of the mask showed that the F3 formula (combination of 1% Moringa leaf extract and 0.30% lemongrass essential oil) produced the best mask preparation. All mask formulas had homogeneous results and met the standards on the viscosity, pH and dispersibility test, but the F1 formula (positive control) on the adhesion test and the F1 formula (positive control) and the F2 formula (negative control) on the drying time test did not meet the requirements good standard of mask. The F3 mask formula is preferred by probandus and all mask formulas do not cause irritation to the skin. Keywords: Cymbopogon nardus L. Rendle, gel, mask, Moringa oleifera Lam., Peel-off
INDEKS MITOSIS UJUNG AKAR KECAMBAH CABE BESAR (Capsicum annuum L.) SETELAH PERLAKUAN SUSPENSI Trichoderma sp. PetroneLa Deno Raja; Eniek Kriswiyanti; Ni Nyoman Darsini
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui indeks mitosis ujung akar kecambah cabe besar (Capsicum annuum L.) setelah perlakuan suspensi Trichoderma sp. Penelitian ini dilakukan di laboratorium Struktur Perkembangan Tumbuhan Jurusan Biologi FMIPA, Universitas Udayana dari Oktober 2013-November 2013. Metode yang digunakan adalah metode squash, biji cabe untuk kontrol direndam dalam air ± 6 jam, untuk perlakuan biji setelah direndam air, direndam lagi dalam suspensi Trichoderma sp. 10-7 selama ± 6 jam, selanjutnya dikecambahkan. Ujung akar kecambah 2 mm dipotong, difiksasi dalam larutan farmer ± 2-24 jam, dihidrolisis dalam larutan 3N HCL ± 2-5 menit dan kemudian pewarnaan dengan aceto orcein ± 5 menit. Pengamatan dilakukan dengan mikroskop binokuler, data pembelahan tiap fase mitosis dihitung (%), dicatat dan difoto, dan dianalisis dengan menggunakan uji paired T tes.Hasil penelitian menunjukkan bahwa Trichoderma sp. berpengaruh terhadap indeks mitosis sel ujung akar Capsicum annuum L.,  pada fase metafase berbeda nyata antara kontrol dan perlakuan, sedangkan pada fase profase, anafase dan telofase berbeda tidak nyata.  Pada perlakuan persentase fase profase, metafase, anafase dan telofase (77,14%; 12,96 %; 5,88 % dan 5,23 %) lebih tinggi dari kontrol (66,40 %; 5,44 %; 4,96 % dan 4,66 %).
PERKEMBANGAN LATISIFER PADA KULTUR KALUS CATHARANTHUS ROSEUS (L) G. DON YANG DIINDUKSI DENGAN KOMBINASI ZAT PENGATUR TUMBUH KINETIN + NAA NI NYOMAN DARSINI
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The development of laticifer on callus culture of Catharanthus roseus (L) G Don in Zenk medium supplemented with combination of plant growth regulator kinetin + NAA was studied. The explants were taken from the second folium from shoot apex. Development of laticifer was observed using descriptive analysis method for callus anatomy and percentage of laticifer was observed during 4–14 weeks of callus development. The percentage of laticifer was determined by counting the average number of the laticifer and the average number of surrounding cells in every optical field of few under light microscope. The results showed that early development of laticifer which was induced with plant regulator growth kinetin + NAA was found in the 9 weeks old callus. The laticifer has specific characteristics i.e. thicker cell wall and longer cell than sorounding cell. Elongated laticifer was observed at 12 weeks old callus. The highest percentage of laticifer on callus C. roseus induced with combination of kinetin and NAA was found in 12 weeks old callus i.e. 0,12%. At 13 and 14 weeks old callus, the anatomy of laticifer was similar to that at 12 weeks old callus, but the percentage was lower.
ANALISIS KEANEKARAGAMAN JENIS TUMBUHAN OBAT TRADISIONAL BERKASIAT UNTUK PENGOBATAN PENYAKIT SALURAN KENCING DI KECAMATAN KINTAMANI, KABUPATEN BANGLI PROVINSI BALI Ni Nyoman Darsini
Bumi Lestari Journal of Environment Vol 13 No 1 (2013)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

AbstractThe research has done on medicinal plants are believed to cure or treat urinary tractdisorders in 15 villages of Kintamani district Bangli. Based on the results of researchthrough the interview process with the public, traders and some interviewees ceraken JeroBalian it is known there are 47 types of plants are believed to overcome the disease urinarytract disorders. Various kinds of urinary tract disorders can be cured by 47 different herbsare-different. Of the 47 plant species are classified as rare plants, three plants namely: pule(Astolnia scholaris), purnajiwa (Euchresta horsfieldii (Lesch) Benn), and suren (Taonasuroni Merr). The dominant plant organ used as medicine are the leaves (59.57%).
JENIS DAN MANFAAT FLORA PULAU DERAWAN KABUPATEN BARAU PROVINSI KALIMANTAN TIMUR Ni Nyoman Darsini; I .G. A. Sugi Wahyuni; A.A.K. Darmadi; Ni Nyoman Wirasiti
Bumi Lestari Journal of Environment Vol 8 No 1 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on identication of plants species available on Derawan Island, Berau Regency, East Kalimantan has been conducted based on exploration survey around the island between 2002-2005. The plants whicht have observed were then listed on a table, their scientific names wrre recorded, their status (whether being protected or not) were determined, and the benefits or use of the plants were also listed. From this study it can be found 45 species of 30 families. They may bring benefits for the locals as vegetables, source of medicine, providing shady spots, or decorative plants.
PERKEMBANGAN MIKROGAMETOFIT DAN UJI VIABILITAS SERBUK SARI KELAPA (Cocos nucifera L. “Ancak”) Ni Luh Gede Cerli Tonika Sari; Eniek Kriswiyanti; Ni Nyoman Darsini
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

The research observed the type of pollen shape, development of microgametophyte, andpollen viability of Cocos nucifera L. “Ancak”, that is the pollen structure Cocos nucifera L. usedpreparation with acetolysis method. Development of microgametophyte, and pollen viabilitywith germinated pollen in vitro used 0,8% of agar media in sugar solution 0%, 50%, and 70%.The result showed that the type of pollen shape Cocos nucifera L. “Ancak”: circular, sulkus,media, Subferoidal, P/E: (1.12-1.18). Development of microgametophyte Cocos nucifera L.“Ancak” the flowers are ripe generally at the level of uninucleate (> 60%) and binukleat (<40%).Male gametes (trinukleat) started on day 2,4. percentage of pollen viability in vitro low average(< 2.5%).Keywords: Pollen structure, microgametophyte, viability test, in vitro
Mansoa alliacea and Allamanda cathartica Extract Formula for The Control of Stem Rot Disease on Soybean Plants Ni Made Susun Parwanayoni Made Susun; Nyoman Darsini
Jurnal Fitopatologi Indonesia Vol 19 No 2 (2023)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.2.57-62

Abstract

Potensi keanekaragaman hayati flora di Indonesia belum dimanfaatkan secara maksimal sebagai pestisida nabati yang ramah lingkungan, dan mengurangi penggunaan pestisida sintetis pada tanaman. Daun Mansoa alliacea dan Allamanda cathartica memiliki potensi sebagai pestisida nabati karena secara in vitro mampu menghambat Athelia rolfsii, penyebab penyakit busuk batang pada tanaman kedelai. Penelitian dilakukan pada skala lapangan untuk menguji formula aplikasi ekstrak daun M.allicea dan A. cathartica yang berpengaruh terhadap perkembangan penyakit busuk batang, pertumbuhan dan produktivitas tanaman kedelai. Tahapan penelitian meliputi: ekstraksi, pembuatan formula dengan mencampurkan ekstrak daun M.allicea dan A. cathartica, aplikasi formula pada tanaman kedelai, pengamatan, dan pemanenan. Formula ekstrak 1.5% daun M. alliacea dan A. cathartica direkomendasikan untuk diaplikasikan karena dapat menekan penyakit busuk batang, meningkatkan pertumbuhan tanaman dan hasil panen di lapangan.
Co-Authors Anak Agung Ketut Darmadi ENIEK KRISWIYANTI Erlina Adhayanti I Gusti Ayu Sugi Wahyuni Junita Hardini MADE SUSUN PARWANAYONI Ni LUH ARPIWI Ni Luh Gede Cerli Tonika Sari Ni Nyoman Wirasiti Ni Putu Ariwathi PetroneLa Deno Raja