Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

1.431

P-Index

This Author published in this journals

All Journal International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences SIMBIOSIS Jurnal Mikologi Indonesia

Junita Hardini

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana. Jl. Raya Kampus UNUD, Jimbaran, Kuta Selatan, 80361, Bali, Indonesia.

Author-ID : 2925726

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Published : 9 Documents Claim Missing Document

Claim Missing Document

Articles

DIVERSITY OF SPECIES CRUSTOSE LICHEN OF Plumeria spp. IN BALI ISLAND Junita Hardini; Rina Sri Kasiamdari; Santosa Santosa; Purnomo Purnomo
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p19

The research of species crustose lichen was conducted in the lowlands of the Bali Island and was conducted randomly at a height of 0-500 m above sea level. The aim is to find out the diversity of species crustose lichen living in Plumeria spp. The method used is descriptive qualitative analysis method. Data collection was done by field observation and specimen collection. The lichen specimens were observed and identified morphologically, anatomically, and chemically. In this study found of four families consisting of six genera and 15 species, namely Graphina sp., Phaeographina sp., Graphis sp., Graphis immersella, G. nilgiriensis, G. modesta, G. nana, and G. conferta (Graphidaceae); Lecanora sp.1, and Lecanora sp.2 (Lecanoraceae); Lepraria sp. (Stereocaulaceae); Caloplaca sp. (Teloschistaceae). The most common species is Graphis sp. (88%).

Keanekaragaman Jenis Tumbuhan Bahan Ramuan “Boreh Basanbuat” Untuk Memperlancar Produksi Air Susu Ibu (ASI) di Bali Enik Kriswiyanti; Ni Nyoman Darsini; Junita Hardini; Ni Putu Ariwathi
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p15

Boreh (bahasa Bali) merupakan salah satu bentuk ramuan tradisional berupa campuran rempah-rempah yang dihaluskan, ditambah cairan (air/arak/minyak kelapa) kemudian dilulurkan pada bagian tubuh. Salah satu boreh yang dapat digunakan untuk memperlancar produksi air susu ibu (ASI) adalah boreh basanbuat. Penelitian ini bertujuan untuk mengetahui jenis dan bagian tumbuhan yang dapat digunakan untuk membuat boreh basanbuat di Bali. Metode penelitian yang digunakan: purposive sampling, pengumpulan data dengan cara wawancara, observasi dan dokumentasi dengan pedagang crakenan, pengobat tradisional (battra) dan masyarakat dari kota dan kabupaten wilayah propinsi Bali, sebanyak 90 responden. Hasil penelitian didapatkan 54 jenis tumbuhan (52 genus, 29 familia) untuk membuat 64 jenis ramuan, jenis tumbuhan yang paling banyak digunakan dari anggota famili Zingiberaceae, bagian tumbuhan yang paling banyak digunakan adalah daun (23,21%). Ramuan boreh basanbuat umumnya menggunakan 5-6 jenis tumbuhan, yaitu menggunakan: beras (Oryza sativa L.), biji kelabet (Trigonella feonum-graecum L.), akar wangi (Androphogon zizanioides (L) Urban), kayu/bubuk cendana (Santalum album L.), daun delem/ nilam (Pogostemon cablin (Blanco) Benth.) dan rimpang kencur (Kaemferia galanga L). Manfaat bahan boreh selain memperlancar produksi ASI, juga untuk aroma terapi, antiinflamasi, anti pembengkakan, antibakteri, antijamur, dan lain sebagainya.

Tumbuhan Pewarna Alami Dan Pengolahannya Pada Tenun Ikat Amarasi Di Desa Tunbaun, Kecamatan Amarasi Barat, Kabupaten Kupang, Nusa Tenggara Timur Fransiska Nitti; Junita Hardini; Made Pharmawati
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p17

This study aims to identify the types of plants, its parts that are used and the processing methods for amarasi weaving fabrics. Data collection in this study were carried out in of the village of Tunbaun, West Amarasi Sub-district, Kupang District, East Nusa Tenggara as one of the centers for the production of amarasi woven fabrics. Qualitative method was used in this study with interview, observation, documentation and literature study techniques. The results showed that the weaving craftsmen in Tunbaun village, used four types of plants as natural dye for weaving including noni root bark (Indigofera tinctoria L., Fabaceae), indigo leaves (Indigofera tinctoria L., Fabaceae), young teak leaves (Tectona grandis L.f., Lamiaceae) and turmeric rhizome (Curcuma domestica Val., Zingiberaceae). Processing of ground noni root bark, then boiled in water to get a red color. Indigo leaves are soaked for 2x24 hours, added lime and let with solution and allowed to stand for 24 to obtain a blue indigo paste. Young teak leaves are boiled in water until they get a purple color. While the turmeric rhizome is grated and then boiled in water to get a yellow color. Keywords: Amarasi, Natural Dyes, Weaving

Potential of Awar-awar Leaf Methanol Extract (Ficus septica Burm. f.) Against Aspergillus niger on Balinese Lontar Ni Komang Deny Julyeda; Ida Bagus Gde Darmayasa; Junita Hardini
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p10

Ficus septica Burm. f. is a wild plant that has antifungal compounds. Exploration of antifungal compounds from natural material important in inhibiting the growth of Aspergillus niger, one of Balinese lontar destructive fungus. Natural material use to reduse lontar damage due to synthetic materials. This study aims to inhibition of awar-awar leaf methanol extract (in vitro). The method used was a complete randomized design with 7 treatments for the concentration of the awar-awar leaf methanolic extract of 10.000, 20.000, 30.000, 40.000, 50.000 ppm (b/v), positive control (nystatin), and negative control (methanol). The minimum inhibitory concentration (MIC) test uses 6 treatments for the concentration of the awar-awar leaf methanolic extract of 100, 500, 1000, 2000 ppm (w/v), positive control (nystatin), and negative control (methanol). Giving the extract to the diffusion well with potato dextrose agar (PDA) media. The research data were analyzed statistically ANOVA (DMRT with 5% significance level) with the SPSS 23 version for windows. The results showed that the awar-awar leaf methanol extract had MIC at a concentration of 500 ppm (w/v). Concentrations of 10.000-50.000 ppm (w/v) indicate the inhibition zone diameter of 20.50 mm, respectively; 23.00 mm; 23.50 mm; 23.75 mm; 24.75 mm. The concentration of 10.000 ppm (w/v) has higher inhibition than the positive control. The concentration of 20.000 ppm (w/v) was the optimum concentration of inhibition of awar-awar leaf methanol extract. Conclusion of this research was awar-awar leaf methanol extract had anti-fungal compounds which inhibited the growth of A. niger. This extract can be used as an alternative to inhibit the growth of fungi that cause lontar damage. Keyword: Aspergillus niger, destructive fungi, Ficus septica Burm. f.

EFFECTIVENESS OF Trichoderma asperellum TKD WITH MEDIATOR OF MANURE TO CONTROL FUSARIUM DISEASE ON RED PEPPER PLANT (Capsicum annum L.) Nyoman Mega Antari; Ida Bagus Gede Darmayasa; Junita Hardini
SIMBIOSIS Vol 8 No 2 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Red pepper is agricultural products in Indonesia which is often attacked by fusarium wilt disease due to the fungus Fusarium oxysporum f.sp. capsici, so in this study aims to control of fusarium wilt disease using Trichoderma asperellum TKD with mediator of manure. Method in this study is RAL (Completely Randomized Design) with 7 treatments P1 = Control I (without treatment); P2 = Control II (F.oxysporum f.sp. capsici (disease)); P3 = disease + T. asperellum TKD 10 mL; P4 = disease + T. asperellum TKD 20 mL; P5 = disease + T.asperellum TKD 30 mL; P6 = disease + T. asperellum TKD 40 mL; P7 = disease + T. asperellum TKD 50 mL. The results showed that T.asperellum TKD was able to inhibit F.oxysporum f.sp. capsici in in vitro by 100%. The highest percentage of fusarium wilt disease (100%) is shown in plants that are only given F.oxysporum f.sp. capsici and without T. asperellum TKD, as well as plant height, total of fresh and dried leaves in plants added by T. asperellum TKD is better compared to plants that are not given T. asperellum TKD. The best plant height, number of freh leaves, and lowest number of dry leaves found in plants with 50 mL of T.asperellum TKD. Keywords: biocontrol, pathogenic fungi, Trichoderma asperellum TKD

Peranan FMA Glomus sp. dan Pupuk Anorganik terhadap Produktivitas Tanaman Kedelai (Glycine max (L.) Merrill) var. Lokal Bali Naomi Anggi Triarta; Meitini Wahyuni Proborini; Junita Hardini
Jurnal Mikologi Indonesia Vol 3, No 2 (2019): Desember 2019
Publisher : Perhimpunan Mikologi Indonesia

Produktivitas tanaman kedelai (Glycine max var. Lokal Bali) dapat ditingkatkan melalui pemupukan. Salah satu pupuk hayati yang bisa diaplikasi sebagai pendamping pupuk anorganik adalah fungi mikoriza arbuskula (FMA). Penelitian ini bertujuan untuk mengetahui peranan FMA Glomus sp. dan pupuk NK terhadap produktivitas tanaman kedelai lokal Bali. Penelitian ini dilakukan di laboratorium Taksonomi Tumbuhan/Mikologi dan Shading house Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana. Penelitian ini menggunakan Racangan Acak Kelompok (RAK) dengan 5 perlakuan yaitu (A) Tanah steril (kontrol) atau tanpa inokulasi FAM dan pupuk NK; (B) Tanah steril, 50 spora Glomus sp.; (C) Tanah steril, 50 spora Glomus sp. dan pupuk NK 5 g; (D) Tanah steril, 50 spora Glomus sp. dan pupuk NK 10 g; (E) Tanah steril, 50 spora Glomus sp. dan pupuk NK 15 g. Variabel yang diamati adalah tinggi tanaman, berat kering tajuk, berat kering akar, jumlah polong isi dan polong hampa, berat basah dan berat kering polong, dan persentase kolonisasi FMA Glomus sp. Hasil penelitian menunjukkan bahwa pemberian 50 butir spora Glomus sp. dan pupuk NK 5 g pada perlakuan C berperan meningkatkan produktivitas tanaman kedelai terlihat pada jumlah polong isi sebanyak 51 polong per tanaman yang berbeda nyata secara statistik dan memiliki berat kering sebesar 9,10 g, tetapi tidak memberikan pengaruh yang berbeda nyata terhadap pertumuhan tanaman kedelai.

DAYA HAMBAT EKSTRAK LENGKUAS PUTIH (Alpinia galanga L.) TERHADAP PERTUMBUHAN Escherichia coli O157:H7 dan Staphylococcus epidermidis Deny Christine Sidabutar; Ida Bagus Gede Darmayasa; Junita Hardini
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Escherichia coli O157:H7 and Staphylocccus epidermidis are bacteria that can cause disease in humans. Alternative control efforts are very important because the use of antibiotics can cause adverse side effects. This study aims to obtain extracts of white galangal (Alpinia galanga L.) in inhibiting the growth of E. coli O157:H7 and S. epidermidis. In this test, white galangal extract was macerated with three solvents at different polarity levels (96% ethanol, n-hexane and ethyl acetate). The ability of white galangal extract in inhibiting bacteria was tested using the Kirby Bauer method (paper disc). The concentration of galangal extract tested was 0%; 5% ; 10% ; 15%; 20% (w/v), control (ciprofloxacin) and MIC (Minimum Inhibition Concentration). To determine the toxicity of the extract, the LC50 test was carried out. Meanwhile, to determine the class of compounds contained in the galangal extract, phytochemical tests were carried out using the phytochemical screening method. The results showed that white galangal extract with 96% ethanol as solvent and n-hexane was able to inhibit the test bacteria. Inhibition against E. coli O157:H7 was most effective in n-hexane solvent with an extract concentration of 20% (inhibition zone diameter 20.2 mm). while for S.epidermidis was n-hexane solvent with a concentration of 20% (inhibition zone diameter 23.5 mm). LC50 against E. coli O157:H7 with 96% ethanol and n-hexane as solvents 11.2% and 10.4%, respectively. Meanwhile, the LC50 of S. epidermidis with 96% ethanol and n-hexane solvents were 9.9% and 9.3%, respectively. Qualitatively, white galangal plant extract contains a class of flavonoid compounds, saponins, terpenoids, and tannins.

PEMANFAATAN Trichoderma spp. SEBAGAI BIOKONTROL Sclerotium rolfsii Sacc. PADA TANAMAN KEDELAI (Glycine max L.) Candra Dewi Oktaviawati; Sang Ketut Sudirga; Junita Hardini
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Sclerotium rolfsii Sacc. on soybeans was one of the diseases in the cultivation that caused a decrease in soybeans (Glycine max L.) production. One of the efforts in increasing the growth and the resistance of the soybean was by using the biological agent named Trichoderma spp. This study aimed to control the intensity of the Sclerotium rolfsii that can cause disease in soybeans. this research was conducted from July 2021 to September 2021 at the Biochemical laboratory and at the Green House owned by the Biology Department FMIPA, Udayana University. The research used an completely randomized design (CRD) with five treatments; a) growing media without the treatment (control); b) growing media + 10 g Trichoderma spp., and without S. rolfsii; c) growing media without Trichoderma spp., + 5 g S. rolfsii; d) growing media + 15 g Trichoderma spp., and 5 g S. rolfsii; e) growing media + 20 g Trichoderma spp., and 5 g S. rolfsii. In this research, the treatment was repeated 5 times and 25 combinations of treatments were obtained. Analysis of Variance (ANOVA) was used in this study to analyze the data, it was found that there was a different result <0,05, then it was continued by using the analysis of Post Hoc Duncan Test. According to the research, it was found that there were two different results. Based on in vitro method, it can be concluded that Trichoderma spp. can inhibit S. rolfsii by about 55,56%. Meanwhile, based on in vivo method, it can be concluded that Trichoderma spp. can inhibit S. rolfsii about 40% which the optimal dose of Trichoderma spp. was about 20 g, the highest plant height was 64.4 cm, the highest average dry weight of shoots was 5.1 g, the highest average dry weight of roots was 0.5 g, and the highest average pod weight was 17.0 g.

Callus Induction In Leucaena (Leucaena leucocephala (Lam.) de Wit) As An Effort To Provide Target Transformation Through Agrobacterium tumefaciens Ni Nyoman Nila Arieswari; Ida Ayu Astarini; Junita Hardini; Austin Ryan Garrido; Debora Margareth; Jennifer Crismonika; Sebastian S. Cocioba
International Journal of Biosciences and Biotechnology Vol 10 No 1 (2022): INTERNATIONAL JOURNAL OF BIOSCIENCES AND BIOTECHNOLOGY
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2022.v10.i01.p02

Leucaena is a plant that produces biomass productivity in the form of hardwood for fuel with low humidity and high calorific value. However, Leucaena is also classified as an invasive plant which can cause the urgency of native plant species and ecosystems in Indonesia. Therefore, the formation of sterile Leucaena needs to be done, one of which is through genetic transformation using Agrobacterium tumefaciens. Callus is used as a target for transformants in the genetic transformation process, so it is necessary to use appropriate media and PGR. This study aimed to determine the type of media and the concentration of 2,4-D on callus induction. This research is an experimental study with a completely randomized design (CRD) method with two factors. The first factor is the type of media (MS and WPM) and the second factor is the concentration of 2,4-D (0; 0.25; 0.50; 0.75; 1.00; 1.25 and 1.50 mgL-1 ). Each treatment was repeated three times so that 42 experimental units were obtained. Parameters observed were callus initiation, callus fresh weight (gram), callus texture and color. Quantitative data is analyzed by analysis of variance (ANOVA). The results showed that the use of media had a significant effect (P<0.05) on callus fresh weight. The use of 2,4- D concentration had a significant effect (P>0.05) on callus texture. The use of WPM media resulted in the fastest callus emergence time (6.67±0.57), the best callus texture (crumb callus type 2) and the best callus color (green). Meanwhile, the highest fresh weight (2,48±0.83) was in the use of MS media. The fastest callus emergence time occurred in the control (without the addition of 2,4-D) (7.33±0.57 and 6.67±0.57), the highest average fresh callus weight (2,48±0.83 and 2.35±0.32) occurred in the treatment with the addition of 1.00 mgL-1 2,4-D with a crumb callus texture of type 2 and callus green color only appeared in the treatment with a concentration of 0.25 mgL-1.

Co-Authors Austin Ryan Garrido Candra Dewi Oktaviawati Debora Margareth Deny Christine Sidabutar ENIEK KRISWIYANTI Fransiska Nitti IDA AYU ASTARINI Ida Bagus Gede Darmayasa Jennifer Crismonika MADE PHARMAWATI Meitini Proborini Wahyuni Naomi Anggi Triarta Ni Komang Deny Julyeda NI NYOMAN DARSINI Ni Nyoman Nila Arieswari Ni Putu Ariwathi Nyoman Mega Antari Purnomo Purnomo Rina Sri Kasiamdari SANG KETUT SUDIRGA Santosa Santosa Sebastian S. Cocioba

Title

Found 9 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 9 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 9 Documents
Search