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All Journal Jurnal Fitopatologi Indonesia METAMORFOSA Journal of Biological Sciences Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS
NI NYOMAN DARSINI
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology

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Journal : Jurnal Biologi Udayana

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INDEKS MITOSIS UJUNG AKAR KECAMBAH CABE BESAR (Capsicum annuum L.) SETELAH PERLAKUAN SUSPENSI Trichoderma sp. PetroneLa Deno Raja; Eniek Kriswiyanti; Ni Nyoman Darsini
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengetahui indeks mitosis ujung akar kecambah cabe besar (Capsicum annuum L.) setelah perlakuan suspensi Trichoderma sp. Penelitian ini dilakukan di laboratorium Struktur Perkembangan Tumbuhan Jurusan Biologi FMIPA, Universitas Udayana dari Oktober 2013-November 2013. Metode yang digunakan adalah metode squash, biji cabe untuk kontrol direndam dalam air ± 6 jam, untuk perlakuan biji setelah direndam air, direndam lagi dalam suspensi Trichoderma sp. 10-7 selama ± 6 jam, selanjutnya dikecambahkan. Ujung akar kecambah 2 mm dipotong, difiksasi dalam larutan farmer ± 2-24 jam, dihidrolisis dalam larutan 3N HCL ± 2-5 menit dan kemudian pewarnaan dengan aceto orcein ± 5 menit. Pengamatan dilakukan dengan mikroskop binokuler, data pembelahan tiap fase mitosis dihitung (%), dicatat dan difoto, dan dianalisis dengan menggunakan uji paired T tes.Hasil penelitian menunjukkan bahwa Trichoderma sp. berpengaruh terhadap indeks mitosis sel ujung akar Capsicum annuum L.,  pada fase metafase berbeda nyata antara kontrol dan perlakuan, sedangkan pada fase profase, anafase dan telofase berbeda tidak nyata.  Pada perlakuan persentase fase profase, metafase, anafase dan telofase (77,14%; 12,96 %; 5,88 % dan 5,23 %) lebih tinggi dari kontrol (66,40 %; 5,44 %; 4,96 % dan 4,66 %).
PERKEMBANGAN LATISIFER PADA KULTUR KALUS CATHARANTHUS ROSEUS (L) G. DON YANG DIINDUKSI DENGAN KOMBINASI ZAT PENGATUR TUMBUH KINETIN + NAA NI NYOMAN DARSINI
Jurnal Biologi Udayana Vol 15 No 2 (2011): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The development of laticifer on callus culture of Catharanthus roseus (L) G Don in Zenk medium supplemented with combination of plant growth regulator kinetin + NAA was studied. The explants were taken from the second folium from shoot apex. Development of laticifer was observed using descriptive analysis method for callus anatomy and percentage of laticifer was observed during 4–14 weeks of callus development. The percentage of laticifer was determined by counting the average number of the laticifer and the average number of surrounding cells in every optical field of few under light microscope. The results showed that early development of laticifer which was induced with plant regulator growth kinetin + NAA was found in the 9 weeks old callus. The laticifer has specific characteristics i.e. thicker cell wall and longer cell than sorounding cell. Elongated laticifer was observed at 12 weeks old callus. The highest percentage of laticifer on callus C. roseus induced with combination of kinetin and NAA was found in 12 weeks old callus i.e. 0,12%. At 13 and 14 weeks old callus, the anatomy of laticifer was similar to that at 12 weeks old callus, but the percentage was lower.
Co-Authors Anak Agung Ketut Darmadi ENIEK KRISWIYANTI Erlina Adhayanti I Gusti Ayu Sugi Wahyuni Junita Hardini MADE SUSUN PARWANAYONI Ni LUH ARPIWI Ni Luh Gede Cerli Tonika Sari Ni Nyoman Wirasiti Ni Putu Ariwathi PetroneLa Deno Raja