Tri Ratnaningsih
Clinical Pathology Departement, Medicine Public Health And Nursing Faculty, Universitas Gadjah Mada

Published : 18 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 18 Documents
Search

Increased Mean Platelet Volume (MPV) in Patients with ST Elevation Myocardial Infarction (STEMI) Compared to Non-ST Elevation Myocardial Infarction (NSTEMI) Patients Tri Ratnaningsih; Istiqomah Istiqomah
Medical Laboratory Technology Journal Vol. 6 No. 2 (2020): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.885 KB) | DOI: 10.31964/mltj.v6i2.320

Abstract

Platelets are known to have a role major in the pathogenesis of atherothrombosis. More giant and hyperreactive platelets accelerate the formation of an intracoronary thrombus. An increased MPV as an indicator of larger and more reactive platelets represents a risk factor for overall vascular mortality, including myocardial infarction. This study aimed to identify the increase of Mean Platelet Volume in Patients with Acute Coronary Syndromes, especially in ST-elevation myocardial infarction and Non-ST-elevation myocardial infarction. Thus this parameter can be used as consideration for diagnosis and treatment decisions. This research is an analytic observational with a cross-sectional method. The subjects were patients with STEMI and NSTEMI who were hospitalized in ICCU Dr.Sardjito Hospital Yogyakarta. Thirty-four subjects STEMI and NSTEMI patients at Dr. Sardjito Hospital, consisting of 28 males (82,35%) and six women (17,65%) with a mean age of 55,5 ± 10,3 years. The results showed that MPV in AMI was higher in patients with STEMI than NSTEMI, but this difference is not significant (p = 0,091). This study concludes that MPV to be higher in patients with STEMI compared to NSTEMI. MPV may be used as a marker of myocardial infarction in clinically appropriate situations.
Iron deficiency screening with content hemoglobin reticulocyte (chr) in children aged 6 months to 5 years Dea Noviana Pramantik; Tri Ratnaningsih; Budi Mulyono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 3 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.082 KB) | DOI: 10.19106/JMedSci004703201501

Abstract

Anemia is a major health problem around the world and iron deficiency is the major cause. In children under 5 years old iron deficiency cause disruption in their growth and development. For that reasons iron deficiency in children should be detected as early as possible. The gold standard for the detection of iron deficiency is hemosiderin examination from bone marrow aspiration, but this examination is invasive and difficult to implement. Ferritin is often used to detect iron deficiency but it has various limitations. Reticulocyte hemoglobin content (CHr) was introduced as a new parameter for the identification of iron deficiency. This parameter measures the levels of hemoglobin in the reticulocyteswhich is newly released from bone marrow and expected to reflect the actualconditions in the bone marrow, therefore it can detect early iron deficiency. It is expected that CHr can identify iron deficiency with cheaper, easy, and applicative method. The aim of this study was to determine the diagnostic performance of CHr in screening of iron deficiency in children aged 6 months to 5 years old. The present study was a cross sectional study to determine the diagnostic performance of CHr and use ferritin as gold standard. The subjects of this study were healthy children aged 6 months-5 years old taken from Posyandu in Yogyakarta district and previously had obtained the consent of their parent/guardian. The CHr examination used a flowcytometry method by Advia120 Hematology Analyzer. The ferritin examination used an electrochemiluminescens method with Elecsys 1010. ROC curve analysis was performed using SPSS version 17. The sensitivity, specificity, positive predictive value, negative predictive value, accuracy, and likelihood ratio were calculated using a 2x2 TABLE. Youden’s index was used to choose the proper cutoff level of CHr for diagnosing iron deficiency. The study was conducted on 104 subjects. Cutoff level of CHr was ≤27.65 pg and the sensitivity, specificity, diagnostic accuracy, positive, and negative predictive values were 91.7%,78.3%, 79.8%, 35.4%, 98.63% respectively. Positive and negative results of likelihood ratios were 4.21 and 0.1. CHr is expected to be used to screen iron deficiencyin children under 5 years old.
Prognostic factors for mortality in patients with severe traumatic brain injury in Yogyakarta, Indonesia Desin Pambudi Sejahtera; Ismail Setyopranoto; Indarwati Setyaningsih; Sri Sutarni; Tri Ratnaningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 2 (2020)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (309.986 KB) | DOI: 10.19106/JMedSci005202202003

Abstract

Determining an accurate prognosis in patients with severe traumatic brain injury (TBI) still becomes a difficult challenge for neurologists. Clinical and laboratory findings have been used as important parameters to establish clinical decisions or even predict future prognosis including death in these patients. We studied the clinical, laboratory and neuroimaging parameters in predicting mortality in patients with severe TBI. This study used the medical records of severe TBI cases in Dr. Sardjito General Hospital, Yogyakarta, Indonesia from January 2015 until July 2016. We evaluated the clinical, laboratory, and neuroimaging examinations of seventy patients with severe TBI in association with mortality. The result showed among 70 patients involved, 35 were dead. Clinical findings of age <40 y.o. (OR=1.143; p=0.015), multiple injuries (OR=5.712; p=0.045), and systolic blood pressure >140 mmHg (OR=3.852; p=0.008) were associated with mortality. Laboratory and neuroimaging parameters of hyponatremia (OR=3.667; p=0.027), hyperkalemia (OR=1.771; p=0.030), and the presence of traumatic subarachnoid hemorrhage (SAH) (OR=6.526; p=0.003) in head CT-scan were significantly associated with mortality. In conclusion, our study showed that productive age <40 y.o. multiple injuries, hyponatremia, hyperkalemia and the presence of traumatic SAH increase the mortality risk in patients with severe TBI.
The Correlation of Cyp2e1 Genetic Polymorphism on Alcohol Drinking Habits in Papuan Ethnicity Mardhatillah Marsa; Yudha Nurhantari; Suhartini Suhartini; Tri Ratnaningsih
Mutiara Medika: Jurnal Kedokteran dan Kesehatan Vol 21, No 2 (2021): July
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/mmjkk.v21i2.11579

Abstract

Alcohol abuse is associated with genetic factors and is influenced by certain races and ethnicities. CYP2E1, which works on the endoplasmic reticulum, produces an enzyme that plays a significant role in alcohol metabolism. In relation to it, this study aims to identify the polymorphisms of CYP2E1*5B and CYP2E1*6 genes in alcohol drinkers of Papuan Ethnics. A total of 39 Papuans were analyzed for alcohol drinking habits. Alcohol drinkers were found to be 29 people (74.4%), and 10 people (25.6%) were non-drinkers. The drinkers mainly were late teenagers (89,7%) and males (69,2%). The CYP2E1*5B genotypes were c1/c1 as 94.9% and c1/c2 as 5.1%. Meanwhile, the CYP2E1*6 T/T genotypes were 56.4%, and T/A genotypes were 43.6%. The odd ratio for CYP2E1*5B were 18,5 and 7,7 for CYP2E1*6. p0,05 for CYP2E1*5B and CYP2E1*6 gene polymorphisms for alcohol drinking behavior in the form of frequency, duration, type, and volume of alcohol consumed. Furthermore, c1/c1 and c1/c2 genotype polymorphisms were in CYP2E1*5B; T/T and T/A genotypes were in CYP2E1*6 of Papuan ethnic at Yogyakarta. In conclusion, genotype c1/c1 had 18,5 times of the possibility of being alcoholic drinkers, and genotype T/T had 7,7 times of the possibility of being alcoholic drinkers in Papuan ethnic. It indicated that the type of genotype statistically did not significantly affect alcohol drinking behavior on the subject.
ACUTE MEGAKARYOBLASTIC LEUKEMIA (AML-M7) IN 10-MONTH-OLD BABY BOY WITH DOWN SYNDROME Tri Ratnaningsih; Aji Bagus Widyantara
Biomedika Vol 14, No 1 (2022): Biomedika Februari 2022
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v14i1.15062

Abstract

ABSTRAKLeukemia megakarioblastik akut (acute megakaryoblastic leukemia, AML-M7) merupakan subtipe AML dengan mayoritas megakarioblas. Kejadian  AML-M7 umumnya terjadi pada anak-anak dengan down syndrome. Bayi laki-laki berusia 10 bulan dengan down syndrome rujukan dari RSU PKU Muhammadiyah Yogyakarta datang dengan keterangan klinis prolonged fever  suspek keganasan akut hematologi seri mieloid. Pemeriksaan tanda vital nadi 102x/menit, suhu  36,7oC pernafasan 30x/menit. Pemeriksaan fisik: berat badan 7,1 kg, tinggi badan  66,1 cm. Hepar teraba 6 cm bawah arcus costae, limpa Schuffner II. Pemeriksaan laboratorium didapatkan lekositosis, netropenia, dan trombositopenia. Pemeriksaan morfologi darah tepi disimpulkan gambaran suspek keganasan akut seri mieloid. Gambaran aspirasi sumsum tulang mendukung diagnosis leukemia megakarioblastik akut atau  acute megakaryoblastic leukemia (AML-M7). Beberapa studi pada kelompok pediatrik menemukan sekitar setengah dari semua kasus AML-M7 terjadi pada anak-anak dengan down syndrome. Pada pemeriksaan fisik terdapat adanya organomegali. Pemeriksaan laboratorium didapatkan anemia, lekositosis, netropenia, dan trombositopenia. Pemeriksaan penunjang lain yang mendukung dalam penegakan diagnosis AML-M7 adalah morfologi darah tepi, aspirasi sumsum tulang, dan pengecatan sitokimiawi. Diagnosis acute megakaryoblastic leukemia (AML-M7) pada bayi dengan down syndrome. Diagnosis didasarkan gambaran klinis, anamnesis (alloanamnesa), pemeriksaan fisik, pemeriksaan laboratorium, dan pemeriksaan penunjang lainnya.Kata Kunci: Acute megakaryoblastic Leukemia, AML-M7, Bayi, Syndrome DownABSTRACTAcute megakaryoblastic Leukemia (AML-M7) is a subtype of AML where megakaryoblasts are the majority of the blasts. The incidence of AML-M7 is commonly seen in children with Down syndrome. A 10-month-old male baby with down syndrome, a referral from PKU Muhammadiyah General Hospital Yogyakarta, came with a prolonged fever suspected of acute hematological malignancy in the myeloid series. Vital signs examination of pulse rates: 102x / minute; temperature: 36.7oC; respiration: 30x / minute. On physical examination, the baby weighs 7.1 kgs, with a height of 66.1 cm. The palpable liver was 6 cm below the arcus rib, and the enlargement of the spleen was classified as Schuffner II. Laboratory tests explained leukocytosis, neutropenia, and thrombocytopenia. The peripheral blood morphology examination showed suspected acute malignancy with myeloid series. Bone marrow aspiration supported the diagnosis of acute megakaryoblastic Leukemia or acute megakaryoblastic Leukemia (AML-M7). Several studies in the pediatric group found that about half of all AML-M7 cases occurred in children with Down syndrome. On physical examination, there was organomegaly. Laboratory tests showed anemia, leukocytosis, neutropenia, and thrombocytopenia. Other supporting investigations in establishing the diagnosis of AMLM7 were peripheral blood morphology, bone marrow aspiration, and cytochemical staining. A diagnosis of acute megakaryoblastic Leukemia (AML-M7) in infants with Down syndrome. The diagnosis was based on clinical features, history (allo-anamnesis), physical examination, laboratory examination, and other supporting investigations. Keywords: Acute megakaryoblastic leukemia, AML-M7, Baby, Down Syndrome
THE DIFFERENCE LEVEL OF MYELOPEROXIDASE IN PLATELET CONCENTRATE BASED ON PREPARATION METHOD AND STORAGE DURATION Fuad Anshori; Teguh Triyono; Tri Ratnaningsih
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 3 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i3.1462

Abstract

The thrombocyte concentrate (TC) preparation process through its storage affects the platelets contained inside. The contaminating leukocytes in TC is an important factor implicated in storage lesion on TC during storage. Leukodepletion is a method to reduce contaminant leukocytes. Myeloperoxidase (MPO) is an enzyme produced by polymorphonuclear cells that have the potential to change structure and function of platelets when there is interaction between them during storage. The aim of this study is assessing the difference in myeloperoxidase level of TC based on its preparation method (leukodepleted and non-leukodepleted) and time storage. A cross-sectional observational study was conducted at the Blood Transfusion Services Unit, Dr. Sardjito hospital, Yogyakarta from April to December 2014. Thrombocyte Concentrate products was grouped based on storage time (≤ and >72 hours) and preparation method (leukodepleted and non-leukodepleted), their MPO was then measured. Mean difference in each group was analyzed using ANOVA test and post hoc test with statistical significance level of p < 0.05. There were 64 eligible subjects, consisted of 29 leukodepleted TCs and 35 non-leukodepleted TCs, based on their storage time, 31 TCs had ≤72 hours storage  time and the other 33 TCs > 72 hours. There were significantly lower median MPO level in ≤72 hours TCs than > 72 hours in non-leukodepleted TC group (13.23 ± 6.47 ng/mL vs 15.58 ± 7.82 ng/mL; p = 0.017). In TC group with more than 72 hours storage time, median MPO level in non-leukodepleted was significantly higher than leukodepleted TC (15.58 ± 7.82 ng/mL vs. 11.11 ± 3.97 ng/mL; p = 0,001). Myeloperoxidase level was lower in non-leukodepleted TC group with ≤ 72 hours than > 72 hours storage time. Furthermore, the MPO level was higher in leukodepleted TC than non-leukodepleted TC in > 72 hours storage time.
Hematology and Iron Status Evaluation Based on Donation Characteristics in Dr. Sardjito Hospital, Yogyakarta Fuad Anshori; Tri Ratnaningsih; Teguh Triyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 27, No 3 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i3.1876

Abstract

Blood donation will reduce iron storage in the body. A high frequency of donations and short interval inter-donations may increase the risk of iron deficiency. In Indonesia, detection of iron deficiency in blood donors is not a routine procedure. Therefore, the comparison of hematology and iron status based on donor characteristics is not widely known. For a month, this study was a cross-sectional study conducted at the Blood Transfusion Service Unit, Dr. Sardjito Hospital. Subjects were routine blood donors who met the criteria for donor selection; however, subjects were excluded if the CRP level was > 10 g/L and had a history of iron supplementation. Subjects were divided based on donation frequency and blood donation interval. The Kruskal-Wallis test was used to compare variables among groups with a statistical significance of p < 0.05. This study involved 145 subjects who met the criteria. Blood donations more than 20 times showed the lowest ferritin levels and iron saturation (16.9 ng/mL and 15.08%). Ferritin levels were also increased in line with the donation interval (35.5 ng/mL; 75.3 ng/mL; 92.7 ng/mL every three months). However, the hematological parameters and iron saturation did not differ significantly based on the donation interval. Hematological parameters are easy and fast procedures but have limitations in the early detection of iron deficiency. Serum ferritin has higher specificity, but its level is affected by inflammatory conditions. Ferritin levels were consistently at the lowest level in the subjects with the highest risk of iron deficiency compared to hematologic and iron saturation parameters.
Abnormal Complex Karyotyping in A Patient Suspected of Acute Myeloblastic Leukemia (AML-M5): A Case Study Purbosari Purbosari; Usi Sukorini; Rahmat Dani Satria; Tri Ratnaningsih; Setyawati Setyawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 28, No 2 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i2.1762

Abstract

Hemophagocytic Lymphohistiocytosis (HLH) is a condition of immune dysregulation characterized by severe organ damage induced by a hyperinflammatory response and uncontrolled T-cell and macrophage activation. Patients with Acute Myeloblastic Leukemia (AML) may be prone to develop HLH. Hemophagocytic lymphohistiocytosis syndrome in AMLpatients with an abnormal complex karyotyping can worsen the patients' prognosis and outcome. A 47-year-old-female presented with prolonged fever, chills, fatigue, weight loss, productive cough, and anemia (blood transfusion (+)). Laboratory findings: hemoglobin 8.5 g/dL, WBC 151.99x103/μL, and platelet count 28x103/μL, peripheral blood 13% blast like cells, 19% promonocytes, 43% atypical (bizarre) monocytes, 25% neutrophils. Levels of CRP>150 mg/L and procalcitonin 82.67 ng/mL, negative HBsAg, and positive IGRA test. Bone marrow morphology showed hypercellularity, decreased thrombopoiesis and erythropoiesis, increased granulopoiesis, macrophages, and hemophagocytosis. Karyotyping results: abnormal karyotypes: 46: XX (9 cells), 44: X (-18), 45: XX (-4), 45: XX (+7, -2, -16), 46: XX (chtb (3), chtb (4), chtb (5), chtb (9), chtb (12), chtb (22)), 46: XX (chtb (5), chtb (7)), 46: XX (chtb (6), chtb (12)), 46: XX (dic 2), 46: XX (chtb (1) (q12), chtb (3) (p21)), 46: XX (chtb (X) (q25) ), 46: XX (der (9), dic (9)), t (9:22)), 46: XX ((+ 21), (-13) chtb (2), p (23), t ( 9:22)). The conclusion was abnormal complex karyotyping. High concentrations of inflammatory cytokines (interleukin-1, interleukin-6, TNF-alpha, and interferon-gamma) secreted by malignant cells and increased phagocytic function of leukemic cells play an important role in the pathogenesis of HLH. Monocytic components (subtypes AML4 and AML5 of the FAB classification) are predisposing factors in cases of AML-related HLH. Cytogenetic abnormalities involving 8p11 and 16p13 are more common in AML-related HLH. Complex genetic abnormalities exacerbate the prognosis of AML, especially in treatment failure. A concluded that was diagnosed with HLH due to AML-M5 with genetic abnormalities of BCR ABL (+), monosomy, trisomy, and multiple chromatid breakage with high mortality. Karyotyping examination is important to determine the prognosis of the disease.
Reticulocyte hemoglobin content (CHr) untuk skrining defisiensi besi pada ibu hamil Bambang Sasangka; Tri Ratnaningsih
Jurnal Gizi Klinik Indonesia Vol 16, No 1 (2019): Juli
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.467 KB) | DOI: 10.22146/ijcn.27387

Abstract

Reticulocyte hemoglobin content (CHr) for iron deficiency screening in pregnant womenBackground: Anemia in pregnancy is a major public health problem in developing countries. In Indonesia, such anemia is generally caused by nutritional deficiencies, particularly iron deficiency. Iron deficiency screening is needed to prevent the development of iron-deficiency anemia in pregnant women that may affect the health of the mother and fetus.Objective: The study objective was to determine the diagnostic validity of reticulocyte hemoglobin content (CHr) for iron deficiency screening in pregnant women.Method: This research is analytic observational research with a cross-sectional design. The subjects were 73 pregnant women in Bantul district with various gestational ages. CHr was examined with an Advia 120 automatic hematology analyzer. Examination of ferritin as gold standard uses chemiluminescens method with Elecsys 1010 immunology analyzer. Table 2x2 is used to test several CHr values in the CHr range for women of reproductive age of 28.8 - 34.5 pg.Results: Based on ferritin <15 μg / L as a gold standard, 13 pregnant women were suffered from iron deficiency while others were normal.Conclusion: CHr at 30.7 pg cut off has a sensitivity of 92.31%; negative predictive value (NPV) 96.2%; and likelihood ratio (LR) produce conclusive changes in post-test probability. In this cut-off, CHr can be used as an iron deficiency screening test in pregnant women.
Pengkayaan ilmu petugas puskesmas sebagai langkah penurunan angka anemia di Kabupaten Sleman Emy Huriyati; Tri Ratnaningsih; Abdul Wahab; Arta Farmawati; Nur Imma Fatimah Harahap; Ainun Nisa
Journal of Community Empowerment for Health Vol 1, No 1 (2018)
Publisher : Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.688 KB) | DOI: 10.22146/jcoemph.38560

Abstract

ABSTRAK Fokus Dinas Kesehatan Kabupaten Sleman untuk penanggulangan anemia dewasa ini bukan hanya pada ibu hamil saja, melainkan sudah dimulai dari remaja puteri, jauh sebelum mereka hamil. Langkah pemerintah dalam menanggulangi anemia dengan adanya suplementasi besi dikhawatirkan tidak tepat sasaran. Sejauh ini deteksi anemia oleh petugas puskesmas hanya sebatas pemeriksaan haemoglobin saja dan kejadian anemia yang terdeteksi masih bersifat general, tidak spesifik anemia defisiensi besi. Subjek dalam kegiatan ini adalah petugas puskesmas meliputi dokter, ahli gizi dan ahli teknologi laboratorium medis yang diambil secara purposive sampling. Metode yang diterapkan dalam kegiatan ini adalah pelatihan dengan rangkaian kegiatan yaitu penyuluhan dengan pre-test dan post-test, tutorial kasus, dan praktikum. Analisis data hasil kegiatan menggunakan uji paired t-test antara nilai pre-test dan post-test. Uji statistik dikatakan signifikan bila p-value kurang dari 0,05. Total peserta yang mengikuti kegiatan adalah 71 orang dari target awal 75 orang (persentase kedatangan= 94,67%). Pelatihan terkait anemia defisiensi besi untuk petugas puskesmas dapat meningkatkan tingkat pemahaman sebesar 32,82 poin (p<0,0001), yang merupakan delta kenaikan nilai pre-test dan post-test. Pemilihan metode untuk kegiatan pelatihan dirasa sudah tepat dengan pertimbangan hasil evaluasi kegiatan menunjukkan sebagian besar peserta kegiatan menilai metode pelatihan efektif atau sangat efektif (74,7%). Program pelatihan dapat meningkatkan pemahaman petugas puskesmas di Kabupaten Sleman terkait anemia defisiensi besi.KATA KUNCI pelatihan; puskesmas; pengetahuan; anemia defisiensi besi ABSTRACT The focus of the Sleman District Health Office in the prevention of anemia is not only on pregnant women anymore as it currently involves young women, long before they become pregnant. The government's step in overcoming anemia with iron supplementation is argued to fail to hit the target. So far the detection of anemia by health center officials is only limited to hemoglobin examination, indicating that the detection of anemia is still general and not specific to iron deficiency anemia. The subjects of this study were community health center staffs including doctors, nutritionists and medical laboratory technology experts who were picked by purposive sampling. The methods used were training with a series of activities: education with pre-test and post-test, case tutorials, and practicum. Paired t-test was performed to compare the difference of value between pre-test and post-test. P-value of less than 0.05 was considered statistically significant. The total participants who took part in the activity were 71 people from the initial target of 75 people (percentage of arrivals = 94.67%). Training related to iron deficiency anemia for health center officers can increase the level of understanding by 32.82 points (p <0,0001). The methods chosen for training activities were considered appropriate with around three-quarters (74,7%) of the participants rated the training method effective or very effective. The training program can improve the understanding of health center staff in Sleman Regency regarding iron deficiency anemia.KEYWORDS training; community health center; knowledge; iron deficiency anemia