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All Journal Biosfer: Jurnal Tadris Biologi Jurnal Biosilampari: Jurnal Biologi Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Jurnal Pengabdian Kepada Masyarakat (JPKM) Tabikpun Journal of Sustainability Science and Technology Organisms: Journal of Biosciences

Eti Ernawiati

Universitas Lampung

Author-ID : 2524802

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Energy Health Professions Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary Other

Published : 8 Documents Claim Missing Document

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Articles

The Effectiveness of Ethanol Extract in Muli Banana Peels (Musa Acuminata) to Heal Cut Wounds in Mice (Mus Musculus L.) Eriola Maulidya; Mohammad Kanedi; Yulianty Yulianty; Eti Ernawiati
Biosfer: Jurnal Tadris Biologi Vol 11, No 1 (2020): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Muli banana peels contain flavonoids, saponins, and tannins that foster the process of wound healing. The purpose of this study was to determine the effect of ethanol extract in muli banana peels (Musa acuminata) on cut wounds healing process in mice (Mus musculus). The study design was the completely randomized design by using 24 male mice which were divided into 6 groups, namely (K+, K-, P1, P2, P3, and P4). Each treatment consisted of 4 mice. All groups of mice were treated with a 1 cm cut wound on the back. The K+ group as the positive control group were applied with povidone-iodine, the K- group as the negative control group were applied with distilled water, the P1 group was applied with a concentration of 10% extract, the P2 group with 20% extract, the P3 group with 30% extract, and the P4 group with 40% extract for 14 days. On the 15th day, histological slides were made by taking 1 sample from each group. The results showed that the ethanol extract of muli banana peels played a role in the cut wound healing process. Uji Efektivitas Ekstrak Etanol Kulit Buah Pisang Muli (Musa Acuminata) Terhadap Penyembuhan Luka Sayat Pada Mencit (Mus Musculus L.)Abstrak: Kulit buah pisang muli mengandung senyawa flavonoid, saponin dan tanin yang memiliki peranan dalam proses penyembuhan luka. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian ekstrak etanol kulit buah pisang muli (Musa acuminata) terhadap proses penyembuhan luka sayat pada mencit (Mus musculus). Rancangan penelitian yang digunakan adalah rancangan acak lengkap, menggunakan 24 ekor mencit jantan yang dibagi menjadi 6 kelompok yaitu (K+, K-, P1, P2, P3, P4) dengan masing- masing perlakuan terdiri dari 4 mencit. Semua kelompok mencit diberi perlakuan luka pada punggung sepanjang 1 cm. Kelompok K+ sebagai kontrol positif dioleskan povidone iodine, kelompok K- sebagai kontrol negatif dioleskan aquades, kelompok P1 dioleskan ekstrak konsentrasi 10%, kelompok P2 dioleskan ekstrak konsentrasi 20%, kelompok P3 dioleskan ekstrak konsentrasi 30%, dan kelompok P4 dioleskan ekstrak konsentrasi 40% selama 14 hari. Pembuatan preparat histologi dilakukan pada hari ke 15 dengan mengambil 1 sampel dari tiap kelompok. Hasil penelitian menunjukan bahwa ekstrak etanol kulit buah pisang muli berperan dalam proses penyembuhan luka sayat yang dilihat dari gambaran histologi lebih baik dibandingkan kelompok kontrol.

Efektivitas Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth) Terhadap Penyembuhan Luka Sayat pada Mencit (Mus musculus L.) Laila Novita Sari; Mohammad Kanedi; Yulianty Yulianty; Eti Ernawiati
Biosfer: Jurnal Tadris Biologi Vol 10, No 2 (2019): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Tujuan dari penelitian ini yaitu mengetahui ekstak etanol daun kenikir (Cosmos caudatus Kunth) terhadap penyembuhan luka sayat pada mencit (Mus musculus L.) dengan konsentrasi yang berbeda yaitu 10%, 15%, 20%, 25%. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL), dengan 6 perlakuan dan 4 kali pengulangan. Terdiri dari K+ dengan povidone iodine, K- dengan Aquades, P1 dengan ekstrak etanol daun kenikir 10%, P2 15%, P3 20% dan P4 25%. Pemberian perlakuan dilakukan pada pagi dan sore hari selama 14 hari. Hasil analisis yang digunakan yaitu uji homogenitas, ANOVA dan uji lanjut BNT pada taraf 5%. Hasil penelitian ini menunjukkan bahwa ekstrak etanol daun kenikir dapat mempercepat penyembuhan pada luka sayat yang dapat dilihat dari gambaran hasil pengamatan histopatologi kulit mencit.

RESISTANCE OF RED CURLY CHILI (Capsicum annuum L.) SPROUTS TO FUSARIUM OXYSPORUM INFECTION FROM SEEDS INDUCED BY 0.2 mT Essy Dumayanti; Rochmah Agustrina; Wawan Abdullah Setiawan; Eti Ernawiati; Yulianty Yulianty; Lili Chrisnawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.167

Curly red chili (Capsicum annuum L.) is widely used as industrial raw material because it has a spicy taste. The increasing demand for chili is not balanced with the level of production. Chilies are susceptible to disease, one of which is fusarium wilt. The magnetic field affected the physical and chemical properties of water so that it increased peroxidase enzyme activity and plant metabolism. This research was aim to know the protective effect seeds induced by 0.2 mT Magnetic Fiel had given to red curly chili (Capsicum annuum L.) sprouts to Fusarium oxysporum infection. This study was arranged in a completely randomized design (CRD) with 5 treatments consisting of M0F0 (control), M7F0, M7F60, M15F0, M15F60. M0 is a seed not induced by a magnetic field; M7 is seed induced by magnetic field 7 minutes 48 seconds, M15 is seed induced by magnetic field 15 minutes 36 seconds, F0 is sprouted not infected with F. oxysporum and F60 is sprouts infected with F. oxysporum for 60 minutes. Each unit is repeated 5 times. The results of the ANOVA showed that exposure to a magnetic field had a significant effect on increasing plant height at 21, 28, and 35 days after planting (HST); the wet and dry weight of 7 days old plants; and the content of chlorophyll a, b, and total before flowering 21 days after planting. Overall, the 0.2 mT magnetic field treatment for 7 minutes 48 seconds tended to give better results to increase the growth of chili plants against F. oxysporum infection.

KAJIAN STRUKTUR ANATOMI DAN MORFOLOGI DAUN PLANLET PISANG KEPOK KUNING HASIL PEMBERIAN EKSTRAK UMBI KEMBANG SUNGSANG SECARA IN VITRO Yoga Aji Saputra; Eti Ernawiati; Rochmah Agustrina; Sri Wahyuningsih
Jurnal Biosilampari : Jurnal Biologi Vol 3 No 2 (2021): Biosilampari
Publisher : LP4MK STKIP PGRI Lubuklinggau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31540/biosilampari.v3i2.1268

Plantain is a fruit commodity that has a high level of market demand, but the yield is low. To overcome this problem, tissue culture techniques are used to increase plantlet production in a short time. Plantain have parthenocarpy character, high sterility, and different levels of ploidy. Tissue culture with the addition of flame lily tuber biomutagen containing colchicine was used as a strategy to overcome the above constraints because it was thought to increase the production of polyploid banana plantlets. Changes in the anatomical and morphological structures are indicators of polyploidy. The purpose of this study was to obtain information on changes in the anatomical and morphological structures of plantain leaves as a result of giving in vitro plantlet propagation media as an indicator of the formation of polyploidy. Plantlets were grown on tissue culture media supplemented with three treatments: 10% flame lily tuber extract, 0.1% pure colchicine, and without addition (control). The parameters observed were epidermal cell size, stomata size, stomata index, number and leaf area. Epidermal cell size, stomata size and stomatal index were analyzed using range values and mean values. The number of leaves, and the average wide of leaf, is then shown in the form of a bar chart. The results showed that administration of flame lily tuber extract was able to increase epidermal cell size, stomata size, wide of a leaf, and able to reduce the stomata index and number of leaves.

Variation of resistance response of orchids induced by Rhizoctonia against the infection of Odontoglossum ringspot virus (ORSV) based on percentage of disease development Fania Nur Izzati; Mahfut Mahfut; Eti Ernawiati; Sri Wahyuningsih
Journal of Sustainability Science and Technology Vol. 1 No. 1 (2021)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/josst.v1i1.3

Orchid is one of the largest groups of flowering plants that are in great demand by society. Besides, the various flowers with a long vase life, the price of orchids is also stable and affordable. Phalaenopsis and Dendrobium are the most popular types of orchids. To anticipate this, orchid cultivation needs to be improved. However, orchid cultivation is not always accompanied by the effort of disease prevention, particularly those caused by viruses. The virus that most infects orchids is the Odontoglossum ringspot virus (ORSV). Symptoms that appear on the leaves are chlorosis, mosaic with a pattern of lines, circles, and necrotic rings/rings. Control of viral infections can utilize organisms that are able to induce resistance, namely mycorrhizae. Mycorrhizae will penetrate into the root cortex tissue and provide nutrients for the orchid. Mycorrhizae that are quite often found are Rhizoctonia and are applied by induction to orchids. Induction was undertaken by planting orchids in Rhizoctonia inoculum until anatomically a peloton structure (solid coils) was formed on the orchid roots. The aim of this study was to study the response of orchid plants in the form of percentage incidence and intensity of the disease and the level of plant resistance to ORSV infection in Phalaenopsis amabilis and Dendrobium discolour which had been induced by Rhizoctonia. This research was conducted at the Laboratory of Botany, Biology, FMIPA, Universitas Lampung, using a factorial completely randomized design (CRD). Factor 1 was the type of orchid and factor 2 was mycorrhizal induction, virus inoculation, and a combination of both with 4 replications. The finding indicated that Phalaenopsis amabilis was more susceptible to ORSV infection than Dendrobium discolour based on a higher incidence and intensity of the disease. Mycorrhizal induction and virus inoculation (MAV) in Dendrobium discolour showed an increase of resistance response compared to Phalaenopsis amabilis.

Induksi Poliploid Planlet Pisang Kepok Batu Dengan Media Kultur Jaringan Amaliya Sabana; Eti Ernawiati; Priyambodo Priyambodo; Rochmah Agustrina
Organisms Vol 2, No 1 (2022): Organisms
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Colchicine is often used in plant breeding to induce mutations and produce polyploid plants with superior traits. Cytologically polylpoid plants have variations in shape, number of chromosomes and larger cell sizes. The use of colchicine in kepok batu bananas is expected to produce polyploid banana kepok batu plants. The purpose of this study was to determine the effect of colchicine compounds on mitotic abnormalities, changes in chromosome number, decreased mitotic index, and the number and length of roots in banana kepok batu plantlets formed on tissue culture media. The research was carried out at the Botanical Laboratory, Department of Biology, FMIPA Unila. This study compared cytology with the squash method between root tip cells of banana kepok batu plantlets after the addition of 0.1% colchicine with controls. Data were analyzed descriptively and displayed in the form of tables and bar charts. The results showed that administration of 0.1% colchicine resulted in mitotic abnormalities (mitotic abnormalities) and increased the number of chromosomes in the control 2n=3x or 33 and 0.1% colchicine treatment, namely 2n=3x+6 or 39. The mitotic index decreased, but averaged – the average number of roots was more and their size was longer than the control. From these results it can be concluded that the administration of 0.1% colchicine was able to induce polyploid cells in banana kepok batu plantlets.AbstrakKolkisin sering digunakan dalam pemuliaan tanaman untuk menginduksi mutasi dan menghasilkan tanaman poliploid dengan sifat unggul. Secara sitologi tanaman polilpoid memiliki variasi bentuk, jumlah kromosom serta ukuran selnya lebih besar. Penggunaan kolkisin pada pisang kepok batu diharapkan dapat menghasilkan tanaman pisang kepok batu poliploid. Tujuan penelitian ini untuk mengetahui pengaruh senyawa kolkisin terhadap abnormalitas mitosis, perubahan jumlah kromosom, penurunan indeks mitosis, serta jumlah dan panjang akar pada planlet pisang kepok batu yang terbentuk pada media media kultur jaringan. Pelaksanaan penelitian di Laboratorium Botani Jurusan Biologi FMIPA Unila. Penelitian ini membandingkan sitologi dengan metode squash antara sel ujung akar planlet pisang kepok batu setelah penambahan kolkisin 0,1% dengan kontrol. Data dianalisis secara deskriptif dan ditampilkan dalam bentuk tabel serta diagram batang. Hasil penelitian menunjukkan bahwa pemberian kolkisin 0,1% memunculkan kelainan mitosis (abnormalitas mitosis) serta meningkatkan jumlah kromosom pada kontrol 2n=3x atau 33 dan perlakuan kolkisin 0,1% yaitu 2n=3x+6 atau 39. Indeks mitosisnya menurun, namun rata – rata jumlah akar lebih banyak dan ukurannya lebih panjang dibandingkan dengan kontrol. Dari hasil tersebut dapat disimpulkan bahwa pemberian kolkisin 0,1% mampu menginduksi sel – sel poliploid pada planlet pisang kepok batu.

Colchicine Effect of Mitosis Root Tip by Banana Kepok Yellow (Musa paradisiaca L.) Polyploid Explants Dellya Vivi Yana; Eti Ernawiati; Rochmah Agustrina; Tundjung Tripeni Handayani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.210

Colchicine can inhibit spindle formation so that doubled chromosomes fail to separate during anaphase cytologically to induce mutations that produce polyploid plants. Yellow kepok banana is a triploid banana. The supply of yellow kepok bananas is hampered by conventional cultivation methods and the limited availability of quality seeds. This study aims to determine the effect of adding colchicine compound to tissue culture media on the mitotic index, chromosome number, and chromosomal abnormalities in yellow kepok bananas and the formation of polyploid plantlets. This study was included in the experimental study by conducting mitotic preparation and observing morphology after the test sample was induced by colchicine. The results of the research that has been done show that the addition of the compound colchicine to the tissue culture medium of yellow kepok banana causes a decrease in the mitotic index, an increase in the number of chromosomes, the presence of chromosomal abnormalities, the formation of polyploid plantlets, and an increase in the number of roots, but there is a reduction in root length.

Penyuluhan Teknik Perbanyakan Tanaman Hias Sebagai Penunjang Perekonomian Keluarga Eti Ernawiati; Tundjung Tripeni Handayani; Sri Wahyuningsih; Mahfut Mahfut
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 4 No. 2 (2023)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v4i2.113

Pemberdayaan ekonomi keluarga merupakan bentuk kepedulian atas permasalahan kemiskinan dan penciptaan lapangan kerja. Ibu-ibu rumah tangga di pedesaan perlu didorong berwirausaha agar dapat menunjang ekonomi keluarga. Wirausaha budidaya tanaman hias cocok bagi ibu-ibu rumah tangga karena berskala rumahan dengan modal kecil. Perbanyakan tanaman merupakan salah satu komponen penting dalam budidaya tanaman hias untuk menjamin ketersediaan bibit berkualitas. Ibu-ibu rumah tangga di Desa Bandar Sari, Kecamatan Padang Ratu, Kabupaten Lampung Tengah sebagian besar berpendidikan rendah sehingga pengetahuan dan keterampilan budidaya tanaman hias mereka terbatas. Oleh karena itu kegiatan ini bertujuan untuk memberikan pengetahuan dan keterampilan teknik perbanyakan tanaman hias. Kegiatan dilaksanakan menggunakan metode ceramah dan demonstrasi. Hasil evaluasi kegiatan yang dilakukan melalui pretest dan posttest menunjukkan peningkatan pemahaman peserta yang cukup signifikan sebesar 76,96 %, yaitu dari nilai rata-rata pretest 48,84 meningkat menjadi 63,46 pada posttest. Sesi diskusi juga berlangsung kondusif dengan pertanyaan-pertanyaan peserta yang menunjukkan keingintahuan yang tinggi untuk memperoleh pengetahuan.

Co-Authors Agustrina, Rochmah Amaliya Sabana Dellya Vivi Yana Eriola Maulidya Essy Dumayanti Fania Nur Izzati Laila Novita Sari Lili Chrisnawati Mahfut Mohammad Kanedi Priyambodo Priyambodo Sri Wahyuningsih Tundjung Tripeni Handayani Wawan Abdullah Setiawan Yoga Aji Saputra Yulianty Yulianty

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