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All Journal AGRIVITA, Journal of Agricultural Science Tropical Wetland Journal JOURNAL OF WETLANDS ENVIRONMENTAL MANAGEMENT
Mariana Mariana
Department Of Plant Protection, Faculty Of Agriculture University Of Lambung Mangkurat
Agriculture, Biological Sciences & Forestry Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Engineering Environmental Science Medicine & Pharmacology Other

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RESISTANCE OF LOCAL BANANA VARIETIES TO FUSARIUM WILT DISEASE Mariana Mariana; Ismed Setya Budi
TROPICAL WETLAND JOURNAL Vol 3 No 1 (2017): Tropical Wetland Journal
Publisher : Postgraduate Program - Lambung Mangkurat University (ULM Press Academic)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/twj.v3i1.44

Abstract

Fusarium wilt is one of the important banana diseases that lead to low quality and quantity of banana production. The objective of this study was to examine and study the resistance of some local banana varieties to fusarium wilt. Pathogens were isolated from the weevil of kepok banana (saba banana) that showed severe symptoms of Fusarium wilt. The inoculum was prepared by propagating the pathogens on Potato Dextrose Agar (PDA) medium. Inoculation was carried out using mycelium fragment suspension (12 mg hyphae mL-1 tween 20 0.05%). The roots of the two-week Ambon banana were injured and immersed in the suspension. Pathogens were determined using Koch's postulates. The varieties tested were seven (7) local banana varieties grown in South Kalimantan. Levels of resistance were determined based on the scale of Leaf Symptom Index (LSI). The pathogens were identified as Fusarium oxysporum f.sp. cubense. The results showed that there were differences in the levels of resistance; Kepok banana (highly susceptible); yellow Ambon, Awa, Susu, Mahuli and Talas bananas (susceptible), and Manggala banana (tolerant).
Potential of Fungi Isolate as a Biological Control of White Root Disease (Rigidoporus sp.) on Rubber Plant Mariana Mariana; Kurnia Utami Dewi; Samharinto Samharinto; Ismed Setya Budi
TROPICAL WETLAND JOURNAL Vol 6 No 1 (2020): Tropical Wetland Journal
Publisher : Postgraduate Program - Lambung Mangkurat University (ULM Press Academic)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/twj.v6i1.82

Abstract

Rigidoporus Sp. is a white root fungus which is the main diseases problems on rubber plants in the wet and dry lands of South Kalimantan. An economically and ecologically beneficial control solution for this problem soil contagion was by using antagonistic fungi. This research was aimed to study the potential of rhizosphere and endophytic fungi in swampland, to control white root fungus on rubber plants. The research used exploratory method. Rhizospheric fungi were explored from swamp rubber plant roots at Pulau Damar village Hulu Sungai Utara District; endophytic fungi were isolated from leaves and jelutung swamps (Dyera lowii) which is rubber-like plants. The isolates found then were screened based on the percentage of inhibition using the dual culture method between pathogens and test isolates, also observed the interaction mechanism and viability test. The research in the laboratory was arranged using a Completely Randomized Design with one factor i.e. the type of antagonistic fungi. Five isolates which had the highest inhibitory power were isolates I13K4R, I7K3R, I1K2R, I6K2R, and I1E with successive inhibitions 95.00%, 83.00%, 76.50%, 62.50% and 53.00%. Thus, isolates have included potential antagonistic agents because they had the ability in space competition more than 50% and proven to be able to paralyze Rigidoporus. The viability test results showed that the isolate that had germination capability of more than 60% were respectively I13K4R 88.05%, I7K3R 87.33%, I1K2R 86.93%, I6K2R 72.85%, and I1E 74.78%.
Antifungal Activities of the Combination of Ulin Wood Liquid Smoke and Hiyung Cayenne Pepper Root Endophytic Fungi Against Colletothricum capsici Witiyasti Imaningsih; Mariana Mariana; Ahmad Budi Junaedi; Rasyidah Rasyidah
AGRIVITA, Journal of Agricultural Science Vol 43, No 1 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v1i1.2458

Abstract

Chili farming faces several constraints, one of which is the pathogenic fungus Colletotrichum capsici. To overcome it can be used indigenous endophytic fungus and lliquid smoke of Ulin (Eusideroxylon zwageri Teijsm. & Binn.) wood which has the potential as antimicrobial can be used. This research aimed to quantify and measure the effectiveness of an antimicrobial liquid smoke, endophytic filtrate, and the combination to suppress C. capsici growth. Subsequently, the research was conducted to apply the liquid smoke, endophytic fungi, and the two combinations of treatments on the growth of C. capsici. Thus, the results of this research showed that liquid smoke with a concentration of 0.085-1.75% can inhibit 3.56-62.17% in range. Meanwhile, the endophytic fungi filtrate, of 2% concentration can inhibit 91.69% C. capsici. Two of the combination liquid smoke in a concentration of 0.68%, 1.36% and the endophytic fungi filtrate in 2% have a demonstrated to inhibit the growth of C. capsici with the highest inhibition into 88.08%. Based on the analysis results, liquid smoke, endophytic fungi filtrate, and a combination of both showed significantly different inhibitory effects between treatments. This indicates that all those three treatments have antimicrobial potential. 
Controlling Anthracnose Disease of Locally Chili in Marginal Wetland using Endophytic Indigenous Microbes and Kalakai (Stenochlaena palustris) Leaf Extract Ismed Setya Budi; Mariana Mariana
Journal of Wetlands Environmental Management Vol 4, No 1 (2016): January-June
Publisher : Center for Journal Management and Publication

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.499 KB) | DOI: 10.20527/jwem.v4i1.51

Abstract

The research aims were to get the indigenus endophytic microbial consortium and to test the potency of kalakai leaf extract as biopesticides and biofertilizer on chili plant specific to wetlands (i.e., var. Hiyung). The microbes capable of inhibiting the growth of anthracnose have been performed on in-vitro test in pairs method.  It was found that 12 isolates have the ability to inhibit the growth of pathogens.  However, based on the results of a confirmatory endophytic test only three isolates had positive role as endophytic in chili plants, namely Trichoderma sp DN3, Trichoderma sp AK2, and Trichoderma sp BT1. The results of the effectiveness of each treatment on chilli plants in the greenhouse and the field shows that the application of endophytic could inhibit the development of anthracnose and spur the growth of plants. It could be concluded that the applications of kalakai leaf extract at the rate of 30 mL/plant can function as biopesticides and biofertilizer.
Co-Authors Ahmad Budi Junaedi Ahmad Budi Junaidi Della Adventaria Ismed Setya Budi Kurnia Utami Dewi Rasyidah Rasyidah Samharinto Samharinto Witiyasti Imaningsih Witiyasti Imaningsih