Article Per Year (5 Year)
p-Index From 2019 - 2024
1.829
P-Index
This Author published in this journals
All Journal Jurnal Kedokteran Syiah Kuala Jurnal Biosains Pascasarjana Jurnal Teknologi Laboratorium Biomolecular and Health Science Journal JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Majalah Biomorfologi (Biomorphology Journal) Indian Journal of Forensic Medicine & Toxicology Cerdika: Jurnal Ilmiah Indonesia
Purwanta, Marijam
Department Of Microbiology, Faculty Of Medicine Universitas Airlangga, Surabaya, Indonesia - Dr. Soetomo General Hospital Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

Published : 12 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 12 Documents
Search

 1   2 
The potency of green grass jelly extract (Premna oblongifolia Merr) as antihyperlipidemia towards aorta histopathology representation of rat (Rattus norvegicus) induced with high fatty diet (HFD) Winthoko, Eka Nora Vitaloka Aprilia Putri; Roosdiana, Anna; Pratama, Dyah Ayu Oktaviani A; Nugraha, Jusak; Purwanta, Marijam; Rifa’I, Muh Husni; Rendy, Achmad Nur
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.174

Abstract

Green grass jelly (Premna oblongifolia merr) is a plant containing fiber and chlorophyll which can lower cholesterol and triglyceride levels. This study have aims to investigate the potency of green grass jelly extract (Premna oblongifolia Merr) to prevent hyperlipidemia. The animal mode used for this study is male Rattus norvegicus, Wistar strain, the age of 8 weeks, and weight of 200 g which is divided into 5 groups of treatment namely group Kn (negative control), Kp (positive control), Kp1, Kp2, and Kp3 induced with HFD and green grass jelly extract at a dose of 5.27 g/ kg BW/ daily, 8.43 g/ kg BW/ daily, 9.37 g/ kg BW/ daily. The data of infiltrating fatty cells and macrophage on aorta histopathology was analyzed by description. This research showed that the treatment of green grass jelly extract (Premna oblongifolia Merr) to animals of hyperlipidemia model reduced infiltration fatty cells and macrophage. The conclusion of this study was the green grass jelly extract was able to prevent an increase of fatty cells and macrophage infiltration of rat (Rattus noervegicus) induced with HFD on dose 9.37 g/ kg/ BW/ daily.
Antibacterial Activity Test of Jatropha multifida L. sap against Staphylococcus aureus and Methicillin Resistant Staphylococcus aureus (MRSA) in vitro Anastassya Yuniar Setyo Putri; Marijam Purwanta; DantiNur Indiastuti; Arthur Pohan Kawilarang
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15611

Abstract

Background: Jatropha multifida L. is a plant that commonly used as a traditional treatment espesciallyfor wounds. It has been proven that its sap had an antibacterial activity against Staphylococcus aureus.Staphylococcus aureus has developed resistant strains named MRSA (Methicillin-Resistant Staphylococcusaureus). Due to this resistance development, hopefully the sap of Jatropha multifida L. can also performedits antibacterial activity against MRSA. Objective: To determine the antibacterial activity of the sap ofJatropha multifida L. against Staphylococcus aureus and MRSA. Methods: A well-diffusion method wasperformed on several concentration: 100%, 50%, 25%, 12.5%, and 6.25%. From this method, the diameterof the inhibition zone produced by the sap was measured. Results: The average of inhibition zone diameterwere 11.29 - 16.00 mm for Staphylococcus aureus and the bigger zone 12.15 - 17.61 mm for MRSA.Conclusion:Jatropha multifida L. had antibacterial activity against Staphylococcus aureus and MRSA. Thisplant showed higher antibacterial activity on MRSA than Staphylococcus aureus.
Efektivitas ekstrak etanol biji kurma mesir (Phoenix dactylifera L.) sebagai antibakteri terhadap Streptococcus pyogenes secara in vitro Lilia Putri Pratama; Marijam Purwanta; Ema Qurnianingsih
Jurnal Kedokteran Syiah Kuala Vol 19, No 3 (2019): Volume 19 Nomor 3 Desember 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v19i3.18113

Abstract

Abstrak. Kurma (Phoenix dactylifera L.) telah dikonsumsi secara luas di seluruh duniadan diketahui memiliki banyak manfaat di bidan kesehatan. Salah satu mafaatnya yaitu sebagai obat terhadap faringitis yang disebabkan oleh bakteri Streptococcus pyogenes. Terdapat ±517.000 kematian pertahun yang disebabkan oleh infeksi Streptococcus pyogenes dan komplikasinya. Sejauh ini telah banyak studi mengenai manfaat daging kurma, namun manfaat terhadap biji kurma belum banyak diteliti. Maka dari itu penelitian ini bertujuan untuk menguji efek antibakteri ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) pada Streptococus pyogenes  secara in vitro dengan uji Konsentrasi Hambat Minimal (KHM) menggunakan metode dilusi serial dan uji Konsentrasi Bunuh Minimal (KBM) dengan inokulasi pada media agar darah. Konsentrasi yang digunakan dalam penelitian ini adalah 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, dan 11,16 mg/ml. Dari penelitian tersebut didapatkan bahwa Konsentrasi Hambat Minimal (KHM) tidak dapat ditentukan dan nilai Konsentrasi Bunuh Minimal (KBM) sebesar 1428.57 mg/ml. Ekstrak etanol biji kurma Mesir (Phoenix dactylifera L.) efektif sebagai antibakteri terhadap Streptococcus pyogenes dengan nilai signifikan 0,002 (p0,05)Kata Kunci: Biji Kurma Mesir, Phoenix dactylifera L., Streptococcus pyogenes, faringitis Abstract. Dates (Phoenix dactylifera L.) are widely consumed in various parts of the world and are known to have many benefits in the health sector. One of them is pharyngitis medication caused by Streptococcus pyogenes. There are ±517.000 deaths per year which caused by Streptococcus pyogenes infection and its complication. So far there have been many studies on the benefits of date flesh, but the benefits of the seeds have not been widely studied. The aim of this study is to test the anti-bacterial effect of the ethanol extract of Egyptian Phoenix dactylifera L. seed on Streptococcus pyogenes in vitro by Minimum Inhibitory Concentration test using serial dilution method and Minimum Bactericidal Concentration (MBC) in blood agar plate inoculates.  The concentration used in this study are 1428.57 mg/ml, 714,29 mg/ml, 357.14 mg/ml, 178.57 mg/ml, 89.29 mg/ml, 44,64 mg/ml, 22,32 mg/ml, and 11,16 mg/ml. From this study the Minimum Inhibitory Concentration (MIC) can not be determined and the Minimum Bactericidal Concentration is 1428.57 mg/ml. Ethanol extract of Egyptian Phoenix dactylifera L. seed effective as antibacteri against Streptococcus pyogenes with a significant value of 0,002 (p 0,05).Keywords: date palm seeds, Phoenix dactylifera L, Streptococcus pyogenes, pharyngitis.
KEPATUHAN 5 WAKTU MENCUCI TANGAN PADA PPDS ILMU BEDAH DI RUANG IRNA BEDAH A RSUD DR. SOETOMO SURABAYA Emeralda Brilian Agnia; Marijam Purwanta; Ariandi Setiawan
Jurnal Kedokteran Syiah Kuala Vol 19, No 1 (2019): Volume 19 Nomor 1 April 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v19i1.18045

Abstract

Abstrak. Infeksi nosokomial merupakan salah satu masalah utama di rumah sakit. Infeksi nosokomial dapat menyebabkan masa tinggal di rumah sakit lebih lama dan peningkatan biaya perawatan kesehatan. Secara umum, infeksi nosokomial dapat ditularkan melalui tiga cara, yaitu infeksi silang, autoinfeksi, dan infeksi lingkungan. Kebiasaan mencuci tangan merupakan intervensi yang paling penting dalam mengendalikan infeksi nosokomial. Desain penelitian ini merupakan penelitian deskriptif dengan mengamati opportunity, indikasi dan action dalam mencuci tangan yang diterapkan. Metode yang digunakan dalam penelitian ini adalah pengumpulan data melalui pengamatan langsung terhadap subyek penelitian yaitu PPDS Ilmu Bedah di ruang IRNA Bedah A RSUD Dr. Soetomo Surabaya sampai memenuhi keriteria 100 opportunity. Dari 100 opportunity, terdapat 144 indikasi mencuci tangan. Indikasi yang paling banyak terjadi adalah saat setelah menyentuh pasien (37,2%). Action yang paling banyak diterapkan adalah handrub dengan menggunakan antiseptik berbasis alkohol (65,2%). Total kepatuhan mencuci tangan pada PPDS Ilmu Bedah di ruang IRNA Bedah A RSUD Dr. Soetomo adalah 23%. Kepatuhan 5 Waktu Mencuci Tangan pada PPDS Ilmu Bedah di ruang IRNA Bedah A pada bulan Oktober 2017 masih sangat rendah. Terdapat beberapa faktor yang dapat mempengaruhi tingkat kepatuhan mereka terhadap mencuci tangan secara tepat dan rutin, sehingga diperlukan intervensi untuk memperbaiki kepatuhan kebersihan tangan dari PPDS Ilmu Bedah tersebut.Kata kunci: 5 Waktu mencuci tangan, Kepatuhan mencuci tangan, Infeksi nosokomial, Pengendalian infeksi nosokomial Abstract. Nosocomial infection is one of  major problems in the hospital. Nosocomial infections may lead to longer hospital stay and increased health care costs. Broadly speaking, nosocomial infections can be transmitted in three ways, namely cross-infection, autoinfection, and environmental infection. The habit of hand hygiene remains the most important intervention in the control of nosocomial infections. This research design was a descriptive research by observing the opportunity, indication and action of hand hygiene applied. The method used in this study was data collection through direct observation of the research subjects namely Surgery Residents in IRNA Bedah A RSUD Dr. Soetomo Surabaya until 100 opportunities were completed. From 100 opportunities, there were 144 indications of hand hygiene. The most prevalent indication was the moment after touching the patient (37,2%). The most preferred technique was handrub using alcohol-based antiseptic (65,2%). Total compliance of hand hygiene in Surgery Residents in IRNA Bedah A RSUD Dr. Soetomo was 23%. Compliance of 5 Moments of Hand Hygiene in Surgery Residents in IRNA Bedah A on October 2017 was still very low. There were several factors that might influence their level of compliance in hand hygiene appropriately and routinely, so interventions were needed to improve the hand hygiene compliance of those Surgery Residents. Keywords: 5 moments of hand hygiene, Compliance of hand hygiene, Nosocomial infection, Infection control.
Bakteri patogen pada telepon genggam mahasiswa fakultas kedokteran Universitas Airlangga angkatan 2014 Ribka Brillyand Angeli Mboe; Marijam Purwanta; Lilik Djuari
Jurnal Kedokteran Syiah Kuala Vol 20, No 1 (2020): Volume 20 Nomor 1 April 2020
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v20i1.11519

Abstract

Recently people use mobile phone in the places that have a high density of microorganisms such as in the hospital, in the kitchen, and in the toilet. Some studied stated that mobile phones played a role in transmission of pathogenic bacteria. Brady et al., reported that 9% to 25% of mobile phones were contaminated with pathogenic bacteria. This research was based on experimental laboratory. The samples were mobile phone, swabbed by wet sterile cotton bud and placed on MacConkey Agar plate and Blood Agar plate. The isolated bacteria were checked by Gram stained. Catalase test for Gram-positive cocci bacteria and specific biochemical test for Gram-negative bacilli bacteria were used to determine the genus of the bacteria. The samples were mobile phone belong to 2014-entering-year students of Medical Faculty of Airlangga University. The sampling method was simple random sampling. This experiment found that 100% of the samples were Staphylococcus spp. positive, 3,3% were Shigella spp. positive and 3,3% were Proteus spp. positive. From this experiment could be concluded that mobile phone  belong to 2014-entering-year students of Medical Faculty of Airlangga University were contaminated by pathogenic bacteria. The result of this experiment found that Shigella spp. and Proteus spp. were pathogenic bacteria on mobile phone belong to 2014-entering-year students of Medical Faculty of Airlangga University.
UJI EFEKTIVITAS EKSTRAK BUNGA KAMBOJA (Plumeria alba) SEBAGAI ANTIBAKTERI TERHADAP Streptococcus pyogenes Felicia Jiwantono; Marijam Purwanta; Yuani Setiawati
Jurnal Kedokteran Syiah Kuala Vol 17, No 3 (2017): Volume 17 Nomor 3 Desember 2017
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v17i3.9066

Abstract

Abstract. infeksi adalah salah satu dari isu kesehatan yang disebabkan oleh mikroorganisme patogen dan salah satu mikroorganisme yang tersering adalah Streptococcus. Terdapat 616 kasus faringitis di dunia yang disebabkan oleh Streptococcus. Penyakit tersebut dapat menimbulkan berbgai komplikasi lain bila tidak diterapi dengan baik. Pengobatan menggunakan antibiotik memiliki risiko terjadinya resistensi. Oleh sebab itu, diperlukan pengembangan penelitian pada herbal sebagai pengobatan. Bunga kamboja putih (Plumeria alba) diketahui memiliki aktivitas antibakteri melawan berbagai macam mikroorganisme. Dengan latar belakang tersebut, tujuan dari penelitian ini adalah untuk menentukan adanya aktivitas antimikroba dari Plumeria alba terhadap Streptococcus pyogenes yang dapat diamati dari Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM). Penelitian ini dikerjakan menggunakan metode eksperimental. Metode dilusi agar digunakan untuk menentukan KHM dan KBM. Konentrasi yang digunakan pada penelitian ini adalah 250 mg/ml, 125 mg/ml, 62,50 mg/ml, 31.25 mg/ml, 15,63 mg/m, 7,81 mg/ml, 3,91 mg/ml, 1,95 mg/ml, 0,98 mg/ml, kontrol positif (+) dan kontrol negatif (-).Melalui observasi dari penelitian ini, KHM tidak dapat ditentukan. Nilai dari KBM adalah 7,81 mg/ml yang menunjukkan konsentrasi dimana tidak didapatkan pertumbuhan koloni bakteri. Ekstrak etanol dari bunga kamboja putih (Plumeria alba) memiliki aktivitas antimikroba terhadapt Streptococcus pyogenes. Dalam studi in vitro ini menggunakan metode dilusi agar, KBM untuk Streptococcus pyogenes adalah 7,81 mg/ml.
Deteksi IgM Anti Salmonella Enterica Serovar Typhi dengan Pemeriksaan Tubex TF dan Typhidot-M Ilham Ilham; Jusak Nugraha; Marijam Purwanta
Jurnal Biosains Pascasarjana Vol. 19 No. 2 (2017): JURNAL BIOSAINS PASCASARJANA
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1130.372 KB) | DOI: 10.20473/jbp.v19i2.2017.127-142

Abstract

Abstrak Bakteri Salmonella enterica Serovar Typhi merupakan bakteri Gram-negatif yang bersifat patogen fakultatif intraseluler, masuk ke dalam tubuh manusia dan menyebabkan penyakit infeksi sistemik akut yang disebut demam tifoid. Deteksi dini antibodi anti Salmonella enterica Serovar Typhi masih merupakan tantangan dalam penegakan diagnosis laboratorium demam tifoid.Tujuan penelitian ini untuk mengidentifikasi perbedaan antara hasil deteksi kit TUBEX TF dan Typhidot-M pada pemeriksaan IgM anti Salmonella enterica Serovar Typhi pasien demam tifoid, menganalisis hubungan suhu tubuh dengan hasil pemeriksaan TUBEX TF, menganalisis hubungan suhu tubuh dengan hasil pemeriksaan Typhidot-M dan menganalisi tingkat kesesuaian hasil deteksi IgM dengan pemeriksaan TUBEX TF dengan Typhidot-M.Penelitian ini merupakan penelitian deskriptif dengan pendekatan observasional, tiga puluh delapan sampel yang berasal dari pasien demam tifoid di RSUD Dr. Soetomo Surabaya.Hasil penelitian ini bahwa kit TUBEX TF menujukkan hasil (65.8%) positif dan (34.2%) negatif. Sedangkan kit Kit Typhidot-M menunjukkan (60.5%) positif dan 15 (39.5%) sampel negatif. Analisis statistik menunjukkan hasil nilai kappa: 0.887>0.75, kedua kit terdapat kesesuaian dengan tingkat kesesuaian sangat baik.Berdasarkan hasil penelitian ini disarankan kit Typhidot-M dapat digunakan sebagai diagnosis cepat bila kit TUBEX TF tidak tersedia. Untuk peneliti selanjutnya disarankan untuk membandingkan hasil TUBEX TF dan Typhodot-M dengan menggunakan kultur darah sebagai diagnosis gold standar untuk deteksi IgM anti Salmonella enterica Serovar Typhi. Kata Kunci: IgM, Salmonella enterica Serovar Typhi, TUBEX TF,  Typhidot-M
Salmonella sp. Detection in Elementary School Street Foods in Surabaya Adelia Anggasta Adzhani; Marijam Purwanta; Ivan Rahmatullah
Biomolecular and Health Science Journal Vol. 1 No. 2 (2018): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.203 KB) | DOI: 10.20473/bhsj.v1i2.9578

Abstract

Introduction: Street foods are food and drink which are processed on the selling spot and/or served as ready-to-consume foods by food vendors to sell to people. Foods and drinks which are rested for too long in the environment will make it possible to get contaminated by pathogen microbes and then results in disease. One of the microbes that is considerably contaminating street foods is Salmonella. We aim to identify Salmonella on drink which are offered in the canteens of Public Elementary School.Methods: This research is design as a descriptive observational, including incubation in Selenite broth, streak in MacConkey agar, and species identification using biochemical tests.Results: There were no Salmonella sp. in drinks sample of street foods which are offered both inside and outside the canteens of Public Elementary School 1 Surabaya, but other gram-negative bacteria contaminated foods.Conclusion: The street foods in elementary school, both the main ingredients and tools used during food processing and serving, still have contamination from pathogen, especially gram-negative bacteria. The vendors and sellers need to be taught how to process and serve foods in better hygiene.
Antibacterial Effects of Fluoride in Streptococcus mutans Growth in Vitro Yura Pradiptama; Marijam Purwanta; Harianto Notopuro
Biomolecular and Health Science Journal Vol. 2 No. 1 (2019): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.927 KB) | DOI: 10.20473/bhsj.v2i1.13232

Abstract

Introduction: Streptococcus mutans is a gram-positive coccus commonly found in the human oral cavity and is a pathogen of dental caries. S. mutans known to form biofilm in infective endocarditis heart-valve. Fluoride usage known to reduce the risk of dental caries. This study aims to analyze how usage of fluoride in inhibiting S. mutans growth.Methods: This study was an experimental study. Antibacterial activity test was performed to evaluate the minimum inhibitory concentration (MIC) using dilution method of sodium fluoride. The minimum bactericidal concentration (MBC) was determined by culturing from the previous dilution test into Chocolate Agar Plate.Results: MIC for sodium fluoride is 4,8 mg/ml and the MBC for sodium fluoride to S. mutans is 4,8 mg/ml. We found S. mutans growth in higher concentration than 19,2 mg/ml. Conclusion: S. mutans was inhibited in dilution test. Growth of the bacteria in higher concentration of sodium fluoride is explained with quasi-irreversible inhibtior effects of fluorida.
NICU Healthcare Workers Hand Hygiene Compliance during 6 Months of Direct Observation Inna Fairuuza Firdaus; Dwiyanti Puspitasari; Marijam Purwanta
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 11 No. 1 (2020): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V11I12020.41-44

Abstract

Introduction: Hospitalized neonates are at risk of developing nosocomial sepsis, a systemic infection due to unhygienic healthcare, especially in Indonesia where healthcare-associated infection incidence rate is 5.9%-8.3%. Hand hygiene by healthcare workers is an effective measure to prevent it. This study aimed to understand the healthcare workers’ hand hygiene compliance towards WHO guidelines 2009 in Dr. Soetomo General Hospital NICU. Methods: This research was an observational study. The healthcare workers’ hand hygiene compliance was obtained through 6 months of direct observation, from November 2018 to April 2019, with doctors and nurses who agreed to be observed and present during the observation period as samples. The observation was recorded with WHO observation form, and processed with Microsoft Excel 2013 to obtain compliance rate. Results: Overall, the compliance of healthcare worker in NICU Dr. Soetomo General Hospital during the 6 months of study was 84%. Nurses’ compliance was higher (87%) than doctors’ (78%). Compliance to My 5 Moments of Hand Hygiene indications varied from 82% for moments before patient contact to 93% for moments after patient contact. Conclusion: In conclusion, hand hygiene compliance of healthcare workers in Dr. Soetomo General Hospital NICU varied between profession categories and each indication. To maintain and improve hand hygiene compliance, intervention in healthcare workers’ behavior is needed, so that healthcare-associated infection can be better prevented.
Co-Authors Adelia Anggasta Adzhani Anastassya Yuniar Setyo Putri Anna Roosdiana Ariandi Setiawan Arthur Pohan Kawilarang Danti Nur Indiastuti Dwiyanti Puspitasari, Dwiyanti Ema Qurnianingsih Emeralda Brilian Agnia Felicia Jiwantono Hana Letitia Anjani Harianto Notopuro Ilham Ilham Inna Fairuuza Firdaus Ivan Rahmatullah Jusak Nugraha Jusak Nugraha Lilia Putri Pratama Lilik Djuari Maftuchah Rochmanti Muhammad Khafidin Al Alim Pratama, Dyah Ayu Oktaviani A Rendy, Achmad Nur Ribka Brillyand Angeli Mboe Rifa’I, Muh Husni Winthoko, Eka Nora Vitaloka Aprilia Putri Yuani Setiawati Yura Pradiptama