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Antibacterial Activity Test of Jatropha multifida L. sap against Staphylococcus aureus and Methicillin Resistant Staphylococcus aureus (MRSA) in vitro Anastassya Yuniar Setyo Putri; Marijam Purwanta; DantiNur Indiastuti; Arthur Pohan Kawilarang
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15611

Abstract

Background: Jatropha multifida L. is a plant that commonly used as a traditional treatment espesciallyfor wounds. It has been proven that its sap had an antibacterial activity against Staphylococcus aureus.Staphylococcus aureus has developed resistant strains named MRSA (Methicillin-Resistant Staphylococcusaureus). Due to this resistance development, hopefully the sap of Jatropha multifida L. can also performedits antibacterial activity against MRSA. Objective: To determine the antibacterial activity of the sap ofJatropha multifida L. against Staphylococcus aureus and MRSA. Methods: A well-diffusion method wasperformed on several concentration: 100%, 50%, 25%, 12.5%, and 6.25%. From this method, the diameterof the inhibition zone produced by the sap was measured. Results: The average of inhibition zone diameterwere 11.29 - 16.00 mm for Staphylococcus aureus and the bigger zone 12.15 - 17.61 mm for MRSA.Conclusion:Jatropha multifida L. had antibacterial activity against Staphylococcus aureus and MRSA. Thisplant showed higher antibacterial activity on MRSA than Staphylococcus aureus.
Diabetes Mellitus and History of Tuberculosis Treatment as Risk Factors of Developing Multidrug-Resistant Tuberculosis at TB Polyclinic Dr. Soetomo General Hospital 2019 - 2020 Muhammad Raihan Habibi; Arief Bakhtiar; Danti Nur Indiastuti; Resti Yudhawati Meliana
Jurnal Ilmiah Universitas Batanghari Jambi Vol 22, No 1 (2022): Februari
Publisher : Universitas Batanghari Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33087/jiubj.v22i1.1908

Abstract

Multidrug-Resistant Tuberculosis (MDR TB) is a condition when Mycobacterium tuberculosis were resistant to Isoniazid and Rifampicin simultaneously, with or without being followed by other first-line Anti-Tuberculosis Drugs. Diabetic patients who also have TB are more susceptible to drug resistance. There is ample evidence noting that a history of previous TB treatment is one of the main factors contributing to the development of MDR TB. This study was a case-control study. The sample of this study was all patients from TB Polyclinic Dr. Soetomo Hospital who were diagnosed with pulmonary TB by pulmonologists on January 1, 2019 – December 31, 2020, who met the inclusion criteria. The data obtained were analyzed using the IBM SPSS Statistics 23 application with a binary logistic regression test.There were 178 samples of this study. MDR TB (65.8%) was the dominant resistance type in Drug-Resistant Tuberculosis (DR TB) patients. Patients with DM were 2.2 times more likely to develop MDR TB than patients without DM. Patients with histories of previous tuberculosis (TB) treatment tended to be three times more likely to develop MDR TB than new patients. Other factors such as age, sex, BMI, history of alcohol consumption, and history of smoking did not show a significant relationship with the incidence of MDR TB. Diabetes Mellitus comorbidity and history of previous Tuberculosis treatment were significant risk factors for developing MDR TB.
Pola resistensi bakteri Pseudomonas aeruginosa dan Acinetobacter baumannii pada spesimen darah terhadap antibiotik golongan β-laktam dan aminoglikosida di Rumah Sakit DR. Soetomo periode Januari 2016 – Desember 2016 Pascalis Fabian; Lindawati Alimsardjono; Danti Nur Indiastuti
Jurnal Kedokteran Syiah Kuala Vol 20, No 1 (2020): Volume 20 Nomor 1 April 2020
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v20i1.18296

Abstract

Abstrak. Pseudomonas aeruginosa dan Acinetobacter baumannii merupakan salah satu penyebab dari infeksi bakteri di rumah sakit. Di seluruh dunia terdapat berbagai kasus yang mana bakteri tersebut memiliki resistensi terhadap antibiotik. Penelitian deskriptif ini bertujuan untuk mengetahui pola resistensi dari Pseudomonas aeruginosa dan Acinetobacter baumannii terhadap antibiotik, khususnya dari golongan β-laktam dan golongan aminoglikosida. Data penelitian ini didapat dari buku log isolat bakteri di Instalasi Mikrobiologi Klinik RSUD Dr. Soetomo pada periode Januari 2016-Desember 2016. Didapatkan hasil dari 63 sampel Pseudomonas aeruginosa yaitu kelompok umur yang paling rentan terhadap infeksi adalah kelompok umur 0-5 tahun (38%) diikuti kelompok umur 45 tahun (23,8%). Serupa, hasil dari 164 sampel Acinetobacter baumannii yaitu kelompok umur yang paling rentan terhadap infeksi adalah kelompok umur 0-5 tahun (32,9%) diikuti kelompok umur 45 tahun (29,9%). Dibagi menurut jenis kelamin, sampel Pseudomonas aeruginosa adalah 55,5% laki-laki dan 45,5% wanita. Untuk sampel Acinetobacter baumannii, 51,2% laki-laki dan 48,8% wanita. Pola resistensi dari Pseudomonas aeruginosa menunjukkan bahwa bakteri secara berurutan telah resisten terhadap ceftazidime (27,4%), gentamicin (24,1%), imipenem (14%), meropenem (11,7%), dan amikacin (4,9%). Sedangkan bakteri Acinetobacter baumannii sudah mengalami resistensi terhadap antibiotik ceftazidime (78,8%), gentamicin (70,1%), amikacin (51,2%), imipenem (49,3%), dan meropenem (48,1%).Kata kunci: Pseudomonas aeruginosa, Acinetobacter baumannii, resistensi, antibiotikAbstract. Pseudomonas aeruginosa and Acinetobacter baumannii are one of the causes of bacterial infection in the hospital. All throughout the world there had been cases of which those bacterias have developed resistance to antibiotics. This descriptive study was aimed towards finding the resistance pattern of Pseudomonas aeruginosa and Acinetobacter baumannii to antibiotics treatment, specifically from the β-lactam group and aminoglycoside group. Data was cited from the isolate logbook of the Department of Clinical Microbiology of Dr. Soetomo General and Teaching Hospital within January 2016 – December 2016. The results from 63 samples for Pseudomonas aeruginosa showed that the most susceptible age groups were within 0-5 years of age (38%) followed by age 45 years (23,8%). Whereas the results from 164 samples for Acinetobacter baumannii the most susceptible age groups were also within 5 years of age (32,9%) followed by age 45 years (29,9%). According to gender, the Pseudomonas aeruginosa samples were 55,5% male and 45,5% female. For Acinetobacter baumannii the samples were 51,2% male and 48,8% female. Resistance patterns showed that Pseudomonas aeruginosa was resistant to ceftazidime (27,4%), gentamicin (24,1%), imipenem (14%), meropenem (11,7%), and amikacin (4,9%). Acinetobacter baumannii was resistant to ceftazidime (78,8%), gentamicin (70,1%), amikacin (51,2%), imipenem (49,3%), and meropenem (48,1%),. In conclusion, both Pseudomonas aeruginosa and Acinetobacter baumannii had shown resistance towards antibiotics, in particular towards ceftazidime, imipenem, meropenem, gentamicin, and amikacin.Keywords: Pseudomonas aeruginosa, Acinetobacter baumannii, Resistance, Antibiotics
Empowering Thalassemia Patients and Family to Increase Public Knowledge on Thalassemia Peter Asa; Danti Nur Indiastuti; Mia Ratwita Andarsini; Jihan Nur Fauziah; Annette d'Arqom
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 7, No 4 (2021): December
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.15 KB) | DOI: 10.22146/jpkm.69349

Abstract

Thalassemia, the fifth-most catastrophic disease with 10,555 patients, causes physical, emotional, and economic burden for the patient, their family, and the country. Annually, IDR 500 billion are needed to cover the treatment of thalassemia. This projected number will continue to increase if no action is taken, so education and knowledge dissemination are important for thalassemia prevention. This community development involves thalassemia patients and families as partners and encourages them to share their knowledge and experience about thalassemia with the Indonesian late adolescents, young adult, and middle-aged adult population through an online webinar to increase public knowledge on thalassemia. Education media, such as posters and videos, were developed and disseminated during the activity, followed by live sharing from thalassemia patients, parents, and pediatricians. The respondents’ knowledge of thalassemia was measured using an 11-items questionnaire before and after the online educational activity. The results show that the respondents have a good knowledge of thalassemia, and it increases after attending online education event about the disease (9.74+2.4, 10.22+0.77, p<0.001). Their knowledge was associated with health-related field experience, knowing someone with thalassemia, and their willingness to undergo thalassemia testing (p<0.046, 0.013, and 0.007, respectively). Thus, these findings support the importance of education and dissemination of information regarding Thalassemia. Moreover, strong knowledge might lead to a willingness to undergo thalassemia testing, which might lead to less marriage between carriers and, eventually, a reduction in the incidence of thalassemia major.
Mortality Comparison of Using Anti Interleukin-6 Therapy and Using Standard Treatment in Severe Covid-19 Jonathan Christianto Sutadji; Agung Dwi Wahyu Widodo; Danti Nur Indiastuti
Folia Medica Indonesiana Vol. 57 No. 2 (2021): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (860.076 KB) | DOI: 10.20473/fmi.v57i2.21929

Abstract

Severe Coronavirus Disease 19 (COVID-19) can cause serious lung inflammation and death. COVID-19 is characterized by a high mortality rate. This severity is associated with the overproduction of proinflammatory cytokines called "cytokine storms". One of the cytokines that play a central role is Interleukin-6 (IL-6). High IL-6 levels are associated with mortality. Expectedly, the IL-6 blockade could reduce cytokine storms and thus reduce deaths in severe COVID-19 patients. This systematic review aimed to summarize the comparison between mortality using anti-IL-6 therapy and mortality using standard treatment in severe COVID-19 patients. We systematically searched the PubMed, ScienceDirect, and ProQuest databases until 13 August 2020. After screening, twelve studies matched the inclusion criteria. The mortality of the anti-IL-6 therapy group was lower than the standard treatment group without anti-IL-6 therapy in COVID-19 patients in 10 of the 12 studies obtained. Four of the ten studies statistically found a significant difference in mortality of the anti-IL-6 therapy group and the standard treatment group. Confirmation of anti-IL-6 therapy effectiveness in reducing mortality in severe COVID-19 patients will require randomized controlled trials.
Edukasi daring untuk pengelolaan kesehatan mental mahasiswa di masa pandemi Covid-19 Natasya Ariesta Selyardi Putri; Nabil Alfin Juhri; Abdurrahman Hasyim Asy’ari; Shofiuddin Al Mufid; Desi Rahmah Sari; Dewangga Sakti Satria Kinasih; Octaviana Galuh Pratiwi; Rezy Ramawan Melbiarta; Gabriel Pedro Mudjianto; Dinda Divamillenia; Danti Nur Indiastuti
Transformasi: Jurnal Pengabdian Masyarakat Vol. 17 No. 2 (2021): Transformasi Desember
Publisher : LP2M Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/transformasi.v17i2.3617

Abstract

[Bahasa]: Pandemi COVID-19 berdampak pada berbagai aspek, termasuk kesehatan mental mahasiswa. Berbagai studi menunjukkan adanya peningkatan kejadian gangguan mental di masa pandemi. Gangguan mental terbanyak adalah depresi, kecemasan, dan stres. Pengetahuan kesehatan mental merupakan salah satu cara untuk menekan kejadian gangguan mental. Namun, data mengenai pengetahuan kesehatan mental terhadap gangguan mental pada mahasiswa masih terbatas. Oleh karenanya, peneliti mengadakan kegiatan edukasi melalui webinar daring untuk memberikan edukasi kesehatan mental yang mencakup penelitian untuk menganalisis tingkat pengetahuan kesehatan mental dan kejadian gangguan mental terbanyak, perbedaan keduanya berdasarkan aspek sosiodemografi, serta hubungan antara dua aspek tersebut pada mahasiswa di Indonesia selama pandemi. Data diolah menggunakan analitik komparatif dan korelasi dengan teknik pengambilan consecutive sampling. Respon diambil menggunakan kuesioner DASS-21 untuk mengetahui status psikologis responden dan 16 item pertanyaan yang telah diuji validitas dan reabilitas untuk mengetahui tingkat pengetahuan responden. Sebanyak 265 responden didominasi oleh jenis kelamin perempuan (81,5%), berusia 15 – 19 tahun (57,4%), dan berasal dari Jawa (62,3%). Sebagian besar responden memiliki tingkat pengetahuan depresi kurang (42,6%), kecemasan baik (61,9%), dan stres sangat baik (48,3%), dengan tingkat depresi normal (40,3%), kecemasan sedang (30,6%), dan stres sedang (27,2%). Hasil uji komparatif tidak ditemukan perbedaan signifikan pada variabel, kecuali tingkat pengetahuan kecemasan menurut kelompok usia (p=0,033). Uji korelasi Spearman Correlation menunjukkan tidak ada hubungan antara tingkat pengetahuan kesehatan mental terhadap tingkat depresi (p=0,250), kecemasan (p=0,819), dan stres (p=0,403). Kata Kunci: kesehatan mental, depresi, kecemasan, stres [English]: COVID-19 pandemic affects many aspects of human life, including college students’ mental health. Some studies showed increase on mental disorder in pandemic era. The most common mental disorders are depression, anxiety, and stress. Mental health knowledge is a means to suppress mental disorder. However, studies that concerned in mental health knowledge on mental disorder amongst college students are limited. Therefore, we held online webinar for mental health education, including a research aimed to analyze the most common mental health knowledge and disorder level, difference of both according to sociodemographic aspects, and correlation between those two aspects in college students during pandemic. All data was processed by using comparative and correlation statistical analytic with consecutive sampling. The instruments used were DASS-21 questionnaire to assess psychological status of respondents and another questionnaire with 16 items that had passed validity and reability tests to assess mental health knowledge. A total of 265 respondents were included, dominated by female (81.5%), aged 15 – 19 years old (57.4%), and from Java (62.3%). Most of respondents had less depression knowledge (42.6%), good anxiety knowledge (61.9%), very good stress knowledge (48.3%), normal depression level (40.3%), moderate anxiety (30.6%), and moderate stress (27.2%). The results of the comparative test show no significant difference on variables, except anxiety knowledge on age group (p=0.033). The result of Spearman Correlation show no correlation on mental health knowledge to depression level (p=0.250), anxiety level (p=0.819), and stress level (p=0.403). Keywords: mental health, depression, anxiety, stress
Analgesic Effect of Bangle Rhizome Ethanol Extraction on Thermally Induced Pain in Mus musculus Margareth Ayu Caroline Pangkerego; Hamzah Hamzah; Danti Nur Indiastuti
Biomolecular and Health Science Journal Vol. 2 No. 2 (2019): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (275.277 KB) | DOI: 10.20473/bhsj.v2i2.15417

Abstract

Introduction: Pain causes deterioration of quality of life. Nonsteroidal anti-inflammatory drug (NSAID) is used to relieve pain with upper gastrointestinal side effects. Zingiber cassumunar Roxb. (known as bangle) is commonly used to relieve pain, but no study has been proved to have analgesic effect. In empirical study, bangle is a potential analgesic due to its bioactive compound named zerumbone. The aim of this study was to investigate the analgesic effect of bangle rhizome ethanol extraction on thermally induced pain.Methods: Five groups of male Mus musculus consisting of 6 mice each were used. A single dose of bangle extract 2 mg/20 g, 4 mg/20 g, 8 mg/20 g, aspirin 1.82 mg/20 g, and aquadest 0.2 ml were administrated. The analgesic effect was evaluated by hot plate test. The test was evaluated at 15th, 30th, 45th minute. Latency period was observed when mice showed pain responses including hind paw shaking, hind paw licking or jumping. The data was analyzed by paired T-test and one-way ANOVA.Results: Results showed that all doses of bangle extract and aspirin 1.82 mg/20 g produced significant latency period (p<0.05) compared to baseline in each group using paired T-test. Latency period of bangle extract 8 mg/20 g was more significant than bangle extract 2 mg/20 g and 4 mg/20 g. One-way ANOVA showed no significant difference in latency period (p=0.1) between bangle extract and aspirin (p>0.05).Conclusion: Bangle extract has analgesic effect on thermally induced pain. The minimum dose for analgesic is 2 mg/20 g or potentially less.
Combination of Red Coconut Coir (Cocos nucifera L. var rubescens) and Linezolid on Methicillin-Resistant Staphylococcus aureus in vitro Growth Trisnawati Trisnawati; Wiwin Retnowati; Danti Nur Indiastuti
Biomolecular and Health Science Journal Vol. 3 No. 1 (2020): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v3i1.19128

Abstract

Introduction: Methicillin-Resistant Staphylococcus aureus (MRSA) bacterial infections may cause poor manifestations and even increase patients’ morbidity and mortality. The use of antibiotics in the management of infections remains the main therapy. However, ever-increasing antibacterial resistance has prompted researchers to find the solution. This study aimed to determine the effect of a combination of red coconut coir (Cocos nucifera L. var rubescens) in various concentrations and linezolid 10 µ/ml on the growth of MRSA bacteria in vitro.Methods: The antibacterial activity test method was well-diffusion test. Bacteria was plated in mueller hinton agar for 24 hours at 37oC. The treatment groups were red coconut coir extract and its combination with linezolid 10 µg/ml.  The well-diffusion test results were statistically analyzed with the One-Way ANOVA and Tukey HSD post-hoc tests.Results: Mean inhibitory zone diameter formed in the C. nucifera L. var rubescens extract test in various concentrations was 12.5 ± 0.36 - 16.2 ± 0.79 mm, while its combination with 10 µg/ml linezolid produced mean inhibitory zone of 15.1 ± 1.31-18.4 ± 0.46 mm. There was a significant difference between groups (p<0.05).Conclusion: Increasing concentration of C. nucifera L. var rubescens extract was in line with the increase of mean diameter of the inhibitory zone. Extract and antibiotic combination tests showed higher mean diameter zones than the single test of materials. The combination of extract and linezolid has the potential to synergistically prevent antibacterial resistance.
Analgesic Effect of Dayak Onion (Eleutherine americana (Aubl.) Merr.) on Mice (Mus musculus) by Hot Plate Test Method Muhammad Hafizh; Danti Nur Indiastuti; Indri Safitri Mukono
Biomolecular and Health Science Journal Vol. 4 No. 1 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i1.26915

Abstract

Introduction: Pain is an unpleasant experience that reduces a person's quality of life. Pain related complain can be treated by administering analgesic drugs. Several studies show that the availability of analgesics is still low, especially opioid analgesics. Dayak onion (Eleutherine americana (Aubl.) Merr.) are used by the Dayaks to relieve pain. Several empirical studies have shown that Dayak onion contain compounds including quercetin as a potential analgesic. This research aimed to investigate the potential analgesic effect of Dayak onion using hot plate method.Methods: The research was conducted experimentally on 36 BALB/c male mice which randomly divided into 6 different treatment groups of Dayak onion exctract, aspirin, codein and aquadest. Each group were thermally pain-induced for latency period measurement by the hot plate test method. Obtained data were processed using Analysis of Variance (ANOVA) followed by Dunnett test.Results: There was a difference in the latency period between the baseline response time and the response time after being treated in each group. ANOVA test results showed significant results (p<0.05) so that the resulting latency period was significant. Dunnett test results showed significant results (p<0.05) in negative control group. Based on these results, Dayak onion are proven to have an analgesic effect on heat stimulation.Conclusion: Dayak onion possess significant analgesic effect on thermally pain-induced mice. Dayak onion extract 90 mg/kg mouse produced better analgesic effects than aspirin 65 mg/kg mouse.
Analgesic Effect Study of Young Coconut Water (Cocos nucifera L.) on Mice (Mus musculus) Induced with Pain using Acetic Acid Dini Indah Berlianti; Danti Nur Indiastuti; Gondo Mastutik; Shaohong Lai
Biomolecular and Health Science Journal Vol. 4 No. 2 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i2.30176

Abstract

Introduction: Pain signals tissue damage that is capable of reducing thequality of life. Non-Steroidal Anti-inflammatory Drugs (NSAIDs) are known as effective analgesic drugs which have various side effects, therefore natural minerals are used as an alternative medicine for pain and inflammation, one of which is known to be coconut water. Hence this research was conducted to find out the differences of the analgesic effect between young coconut water (Cocos nucifera L.) with non-selective and COX-2 selective NSAID on mice induced with pain from acetic acid 0.6% 1 ml/100gBW of mice.Methods: True experimental, conducted at the Pharmacology Laboratory in Faculty of Medicine of Airlangga University involving the sample of 48 mice (6 groups). The recorded data was tested using the oneway ANOVA methodology before then continued with the posthoc test of LSD.Results: The addition of young coconut water (Cocos nucifera L.) with the dosage of 3 ml/100gBW, 4 ml/100gBW, and 4.5 ml/100gBW of mice doesn't give any significant analgesic effect even though the analgesic protection percentage increases accordingly to its dosage (12.32%, 18.72%, 26.88%), but non-selective and COX-2 selective NSAID give significant analgesic effect (p<0.05) on mice induced with pain from acetic acid 0.6% 1 ml/100gBW of mice.Conclusion: There are differences in the analgesic effect of young coconut water (C. nucifera L.) with non-selective and COX-2 selective NSAID on mice induced with pain from acetic acid 0.6% 1 ml/100gBW of mice.