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Ema Damayanti

Unknown Affiliation

Author-ID : 259403

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Articles

The use of earthworm meal (Lumbricus rubellus) as anti-pullorum agent in feed additive of broiler chicken Ema Damayanti; Ahmad Sofyan; Hardi Julendra; Tri Untari
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The aim of this research was to study the use of earthworm meal (TCT) L. rubellus as anti pullorum agent in poultry feed additive (IP). The antibacterial activity of TCT against Salmonella pullorum was examined using diffusion agar method at each of the following concentrations: 0, 25, 50, 75 and 100% (w/v) in 100 µL DMSO. In vivo test was conducted using 80 broiler chicken and were infected by S. pullorum with treatments of: IP0: IP contained 0% TCT, IP1: IP contained 25% TCT, IP2: IP contained 50% TCT, IP3: IP contained 75% TCT and IP4: IP contained 100% TCT. Each treatment was replicated 4 times with 4 chicks each. Feed additive was periodically fed to broiler during 7 days before and 10 days after infection. Anti-pullorum activities were evaluated using serology test, isolation and biochemical identification of S. pullorum. The results showed that 75% TCT was optimum to inhibit S. pullorum in vitro. The isolation and identification of S. pullorum results showed that 0 out of 8 (0%) broilers treated with IP4 was not infected by S. pullorum whereas 1 out of 2 (50%) broilers treated with IP0 were infected by S. pullorum. The reduction of S. pullorum prevalence as followed by increasing TCT in feed additive. In conclusion, TCT as poultry feed additive could inhibit S. pullorum infection. Key words: Earthworm Meal, Feed Additive, S. Pullorum

Antibacterial activity and retained protein of earthworm meal (Lumbricus rubellus) as feed additive combined with chitosan Ahmad Sofyan; Ema Damayanti; Hardi Julendra
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

This research was conducted to enhance the bacterial growth inhibition of E. coli by using earthworm (Lumbricus rubellus) meal (TCT) which was added with chitosan and its effect on the retained protein in broilers. Inhibition of E. coli growth was tested using dilution method on the nutrient broth by additional 2% TCT combined with 0, 0.5, 1.0 and 1.5% chitosan. Retained protein was measured using broiler fed diet containing 2% TCT (w/w) and added by chitosan 0, 0.5, 1.0 and 1.50% of TCT (w/w). The numbers of 15 broilers Cobb strain 35 days old were arranged on Completely Randomized Design (CRD). Results showed inhibition of E. coli was increased using TCT mixed chitosan. The highest inhibition to E. coli growth obtained from TCT + 0.5% chitosan. Retained protein tended to increase up to 1% (w/w) chitosan. Otherwise, chitosan level more than 1% could reduce protein retention. It is concluded that use of 1% chitosan increased TCT capability to inhibit E. coli and protein retention in the broilers. Key Words: Feed Additive, Chitosan, L. rubellus, E. coli

16s rRNA Identification of Pediococcus spp. from Broiler and Studies of Adherence Ability on Immobilized Mucus Ema Damayanti; Lies Mira Yusiati; Achmad Dinoto
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

The objectives of this research were to study taxonomical status of lactic acid bacteria (LAB) isolated from broiler and adherence ability on mucus in vitro. Molecular analysis was performed by analyzing 16S rRNA gene using universal primer. The adherence assay on mucus was carried out using microplate method with total plate count (TPC), absorbance (A550) and confirmed by scanning electron microscopy (SEM). The results of this studies revealed that three of LAB isolates have closed relation to Pediococcus acidilactici (99.9%) species.Three isolates of P. acidilactici have adherence ability on broiler mucus higher than that on porcine mucin with an adherence percentage of 55.5% versus 50.8% and absorbance A550 of 0.061 versus 0.051, respectively. The highest adherence ability showed by P. acidilactici R02 with adherence percentage was 59.3% and absorbance A550 = 0.068. Adherence on mucus were affected by the addition of 3 g/l of gastric juice and 0.3% (b/v) of bile salt. Adherence analysis using SEM also showed that the adherence on broiler mucus was higher than the adherence on porcine mucin. Altogether this adherence studies, suggest that three isolates of P. acidilactici LAB were capable of colonizing host intestinal mucus in vitro as important property to be promising probiotic bacteria for broiler.Key words : adherence, broiler, Pediococcus, mucus, 16S rRNA

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