Hardi Julendra
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The use of earthworm meal (Lumbricus rubellus) as anti-pullorum agent in feed additive of broiler chicken Ema Damayanti; Ahmad Sofyan; Hardi Julendra; Tri Untari
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.826 KB) | DOI: 10.14334/jitv.v14i2.348

Abstract

The aim of this research was to study the use of earthworm meal (TCT) L. rubellus as anti pullorum agent in poultry feed additive (IP). The antibacterial activity of TCT against Salmonella pullorum was examined using diffusion agar method at each of the following concentrations: 0, 25, 50, 75 and 100% (w/v) in 100 µL DMSO. In vivo test was conducted using 80 broiler chicken and were infected by S. pullorum with treatments of: IP0: IP contained 0% TCT, IP1: IP contained 25% TCT, IP2: IP contained 50% TCT, IP3: IP contained 75% TCT and IP4: IP contained 100% TCT. Each treatment was replicated 4 times with 4 chicks each. Feed additive was periodically fed to broiler during 7 days before and 10 days after infection. Anti-pullorum activities were evaluated using serology test, isolation and biochemical identification of S. pullorum. The results showed that 75% TCT was optimum to inhibit S. pullorum in vitro. The isolation and identification of S. pullorum results showed that 0 out of 8 (0%) broilers treated with IP4 was not infected by S. pullorum whereas 1 out of 2 (50%) broilers treated with IP0 were infected by S. pullorum. The reduction of S. pullorum prevalence as followed by increasing TCT in feed additive. In conclusion, TCT as poultry feed additive could inhibit S. pullorum infection. Key words: Earthworm Meal, Feed Additive, S. Pullorum
Antibacterial activity and retained protein of earthworm meal (Lumbricus rubellus) as feed additive combined with chitosan Ahmad Sofyan; Ema Damayanti; Hardi Julendra
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.843 KB) | DOI: 10.14334/jitv.v13i3.586

Abstract

This research was conducted to enhance the bacterial growth inhibition of E. coli by using earthworm (Lumbricus rubellus) meal (TCT) which was added with chitosan and its effect on the retained protein in broilers. Inhibition of E. coli growth was tested using dilution method on the nutrient broth by additional 2% TCT combined with 0, 0.5, 1.0 and 1.5% chitosan. Retained protein was measured using broiler fed diet containing 2% TCT (w/w) and added by chitosan 0, 0.5, 1.0 and 1.50% of TCT (w/w). The numbers of 15 broilers Cobb strain 35 days old were arranged on Completely Randomized Design (CRD). Results showed inhibition of E. coli was increased using TCT mixed chitosan. The highest inhibition to E. coli growth obtained from TCT + 0.5% chitosan. Retained protein tended to increase up to 1% (w/w) chitosan. Otherwise, chitosan level more than 1% could reduce protein retention. It is concluded that use of 1% chitosan increased TCT capability to inhibit E. coli and protein retention in the broilers. Key Words: Feed Additive, Chitosan, L. rubellus, E. coli
Penggunaan Tepung Cacing Tanah (Lumbricus rubellus) sebagai Aditif Pakan terhadap Penampilan Produksi Ayam Pedaging, Profil Darah dan Kecernaan Protein (The Effect of Earthworm (Lumbricus rubellus) Meal as Feed Additive on Broiler Production Performance, Hardi Julendra; Zuprizal (Zuprizal); Supadmo (Supadmo)
Buletin Peternakan Vol 34, No 1 (2010): Buletin Peternakan Vol. 34 (1) Februari 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v34i1.103

Abstract

The objective of this research was to study the use of earthworm meal (Lumbricus rubellus) (TCT) as feed additive on broiler performance, blood profile and protein digestibility. The experiment used 100 day old chicken of cobb strain (CP 707), for 35 days feeding trial. The birds were randomly divided into four dietary treatments (R0: without TCT addition, R1: 0.5% TCT addition, R2: 1% TCT addition, R3: 1.5% TCT addition) with five replications, wich consisted five chickens each. The experimental design used Completely Randomized Design (CRD) of one way ANOVA then continued with Duncan test. Performance test was conducted by recording body weight gain, feed consumption and feed efficiency. Blood profile test was obtained by counting the number of erythrocyte, leukocyte and lymphocytes, whereas protein digestibility test used the collection method of excreta and ileum content. The result showed that feed consumption of R1 (2,065 g), R2 (2,025.4 g), R3 (2,075.3 g) were significantly different (P<0.05) fromR0 (2,309.8 g), while the body weight gain were not different among treatments and FCR of R1 (1.78), R2 (1.78), R3 (1.78) were significantly different (P<0.05) from R0 (2.04). The result of blood profile test showed that the number oferythrocyte among treatments were not significantly different, the number of leukocyte of R1 (18,575 μl), R2 (18,200 μl), R3 (20,175 μl) were found significantly higher (P<0.05) than R0 (16,675 μl) and lymphocytes of R1 (22.7%), R2 (21.8%), R3 (23.6%) were significantly higher (P<0.05) than R0 (15.2%). Protein digestibility of R3 (91.69%) was significantly different (P<0.05) from R0 (81.75%), R1 (90.48%) and R2 (85.99%), while R1 (90.48%) was significantly different (P<0.05) from R0 (81.75%), but R2 (85.99%) was not significantly different from R0 (81.75%). It could be inferred that earthworm meal addition as feed additive could improved production performance of broiler, the poultry health through blood profile, and enhanced the protein digestibility.(Key words: Feed additive, Earthworm meal, Broiler)