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All Journal As-syifaa Jurnal Farmasi

Fitriana Fitriana

Fakultas Farmasi Universitas Muslim Indonesia, Makassar

Author-ID : 2645011

Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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ISOLASI BAKTERI PENGHASIL SELULOSA DARI BUAH-BUAHAN DIPASAR TRADISIONAL MAKASSAR Herwin Herwin; Fitriana Fitriana; Ayyub Harly Nurung
As-Syifaa Jurnal Farmasi Vol 12, No 1 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Isolation of bacterial cellulose from fruits in traditional market of Makassar have been done. The objective of this research is to adding diversity of microorganism that can producing cellulose and observe it’s both optimum temperature and pH. First step is isolation of bacteria from Watermelon, Papaya, Cantaloupe, Mango, Dragon fruit, and Banana that found in traditional market in Makassar using Hestrin-Schramm (HS) agar. Each colony bacteria have been separated to making pure culture using HS agar then the colony was screening in HS broth to obtain colony bacteria that producing cellulose. Screening test found that 10 isolate that can produce cellulose which is MB-B02, MS-B01, MS-B03, NB-B03, PB-B01, PB-B02, PB-B03, PB-B04, PS-B01, and PS-B03. Then the colony that producing cellulose was optimize its temperature and pH. Cellulose obtained from optimization was measure it’s weight to determine optimum temperature and pH. Based from optimization found that 250C was optimum temperature for MS-B01, MS-B03, NB-B03, dan PB-B04, and 370 was optimum temperature for MB-B02, PB-B01, PB-B03, PS-B01, dan PS-B03, meanwhile for isolate PB-B02 optimum both in 250C dan 370C dan pH optimum for all isolate is pH 6

PENELUSURAN FUNGI ENDOFIT SEBAGAI PENGHASIL SENYAWA ANTIBIOTIKA DARI DAUN NANAS (Ananas comosus (L) Meer) Fitriana Fitriana; St. Maryam; Tadjuddin Naid; Maryana Maryana
As-Syifaa Jurnal Farmasi Vol 8, No 1 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

A search endophytic fungi from the leaves of pineapple (Ananas comosus (L) Merr) as a producer of antibiotic compounds will be carried out in order to obtain the endophytic fungi that have the ability to produce bioactive compounds useful as antibiotics.. Results of endophytic fungi isolates obtained six fungal isolates (IJA 1, IJA 2, IJA 3, IJA 4, IJA IJA 5 and 6), which have a different macroscopic characterization of forms, edge and elevation colonies. Isolates pure microbial fermentation results in the test antibiotic activity against some bacteria using agar diffusion test. All isolates pure fermentation results showed inhibitory activity against all bacteria in test as Bacillus subtilis, Esherichia coli, Pseudomonas aeruginosa, Salmonella thypi, Shigella dysenteriae, Staphylococcus aureus, Streptococcus mutans, and Vibrio cholerae. The inhibition of fungi isolates shown in (IJA 4) with a 26 mm zone of inhibition against Esherichia coli bacteria.

PROFIL BIOAUTOGRAM AKTIVITAS ANTIBAKTERI EKSTRAK ISOLAT JAMUR LAUT PADA ALGAE Kappaphycus alvarezii SECARA KLT BIOAUTOGRAFI Fitriana Fitriana; Rusli Rusli
As-Syifaa Jurnal Farmasi Vol 9, No 2 (2017): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

The Research Chromatogram Profile Of Activities Antibacterial Extracts Isolates Marine Fungi in Algae Kappaphycus alvarezii by TLC Bioautography has been done with the intention of doing the screening test of pathogenic bacteria that can be inhibited by the extract of F3 (extract liquid culture and extract mycelia) and extracts of E (extract liquid culture and extract mycelia ) and see chromatogram profile of liquid culture extract of F3 on bacteria Escherichia coli and mycelia extract of E on bacteria Bacillus subtilis. This research was conducted purification of Fungi isolates E and F3 followed by fermentation process of fungi isolates for 3 weeks. Then proceed with the extraction process using an ethyl acetate solvent. The solvent was evaporated to obtain a dry extract, followed by a screening test of antibacterial activity against bacterial pathogenic, antibacterial activity test by TLC Bioautography to liquid culture extract of F3 and extract mycelia of E. The results of the screening test with a concentration of 250 µg was obtained to extract liquid culture of F3 and E provide activity against 5 pathogenic bacteria to extract mycelia while F3 and E provide activity against two bacterial pathogens. Separation of compounds by TLC using the ethyl acetate eluent: ethanol: water (8: 2: 1). The result of TLC Bioautography test of liquid culture extract of F3 to bacteria Escherichia coli and mycelia extract of E on bacteria Bacillus subtilis showed the existence of antibacterial activity.

UJI AKTIVITAS ANTIOKSIDAN ISOLAT FUNGI ENDOFIT DAUN GALING-GALING (Cayratia trifolia L.) DENGAN METODE 1,1-DIPHENYL-2-PICRYLHYDRAZIL (DPPH) Magfirah Abdullah; Fitriana Fitriana; St. Maryam
As-Syifaa Jurnal Farmasi Vol 12, No 2 (2020): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jifa.v12i2.617

Endophytic fungi generally produce secondary metabolites that have beneficial biological activities such as anticancer, antiviral, antibacterial or antioxidant compounds. The aim of this study was to examine the antioxidant activity of galling-galing leaf endophytic fungi isolate (Cayratia trifolia L.) by 1,1-diphenyl-2-picrylhydrazil (DPPH) method. This study used IDGG-03 endophytic fungi isolate fermented for 21 days using MYB medium then extracted and evaporation was carried out. The fermentate extract obtained was then tested qualitatively and quantitatively for antioxidant activity. Qualitative results using TLC plates indicate that galling-leaf fungi isolates have antioxidant activity which is characterized by the presence of yellow spots on a purple background. And in the quantitative test IC50 68,133 µg / mL was obtained, which indicated the antioxidant activity of galling-vane leaf endophytic fungi isolate (Cayratia trifolia L.) classified as a strong antioxidant.

PENENTUAN WAKTU OPTIMUM PRODUKSI METABOLIT SEKUNDER ISOLAT BAKTERI ACTINOMYCETES DARI TANAH RHIZOSFER AKAR TANAMAN JARAK PAGAR (Jatropha curcas. L) TERHADAP BAKTERI PATOGEN Fitriana Fitriana; Rusli Rusli
As-Syifaa Jurnal Farmasi Vol 10, No 1 (2018): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

The study of optimum time determination of secondary metabolite production of Actinomycetes bacteria isolate from Jatropha curcas L root of the soil (Jatropha curcas (L) of pathogenic bacteria has been done with the aim to determine pathogen bacteria that can be inhibited by Actinomycetes bacteria and determine the optimum time in producing secondary metabolite to pathogenic bacteria. The first phase of this study is to isolate Actinomycetes purification IBPT 01. Then performed a screening test of activity against pathogenic bacteria. Furthermore, the timing of production of antibiotics or secondary metabolites with bacterial fermentation Actinomycetes IBPT 01 begins on the 1st day until the 28th day and carried out the activity of antibiotics for 28 days. The results obtained in the screening test Actinomycetes bacteria activity IBPT code 01 provides antibacterial activity with category which has a diameter of 20 mm 11- barriers against 8 bacteria while one bacterium with diameter> 20 mm including strong category. Optimization results showed that the isolated bacteria Actinomycetes capable of producing secondary metabolites on 9 day, 8 day, 7 day, 4 day and 26 day. From this research can be concluded that the bacterial isolates of Actinomycetes IBPT 01 can provide the optimum time to produce different secondary metabolites.

PENENTUAN AKTIVITAS ANTIBAKTERI EKSTRAK ISOLAT FUNGI ENDOFIT DARI DAUN GALING-GALING (Cayratia trifolia L) Ayyub Harly Nurung; Fitriana Fitriana; Herwin Herwin
As-Syifaa Jurnal Farmasi Vol 14, No 1 (2022): AS-SYIFAA JURNAL FARMASI
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jifa.v14i1.785

Utilization of wild plants around us needs to be done to expand the chances of wild plants being used as traditional medicine. These plants have the main content of the active compounds in the form of steroids, terpenoids, flavonoids, tannins and is reported to have antibacterial activity. This research aims to determine the concentration of endophytic fungi isolates IDGG 02, IDGG 03 and IDGG 06 from the leaves of galing-galing (Cayratia trifolia L). The first stage purification isolates of endophytic fungi in order to obtain single isolates. Then fermented using a shaker for 21 days at room temperature. Fermented extracted with ethyl acetate solvent using a liquid-liquid extraction methods to obtain a dry extract. Testing the minimum inhibitory concentration (MIC) with concentrations of 2048, 1024, 512, 256, 128, 64, 32, 16, 8 and 4 µg / mL and testing for antibacterial activity using agar diffusion methods against bacteria Staphylococcus aureus, Staphlococcus epidermidis, Escherichia coli and Vibrio cholerae.The results obtained MIC 2048 MIC ug / mL to extract isolates IDGG 02 and IDGG 06, while the extract obtained 03 isolates IDGG 1024 MIC ug / ml against all bacteria. Results of testing the antibacterial activity of endophytic fungi isolated extract IDGG 02, 03 IDGG produce diameter zone of inhibition at a concentration of 2048 mg / mL against Staphylococcus aureus, Staphlococcus epidermidis, Escherichia coli and Vibrio cholerae.

Co-Authors Herwin Herwin Magfirah Abdullah Maryana Maryana Nurung, Ayyub Harly Rusli Rusli St. Maryam Tadjuddin Naid

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