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Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle Maharani, D.; Fathoni, A.; Sumadi, S.; Hartatik, T.; Khusnudin, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.87-93

MC4R gene is known as an important candidate gene for the growth trait. The purpose of this research was to identify the MC4R gene in Kebumen Ongole grade cattle and examine its association with growth traits. Data of birth weight (BW), weaning weight (WW), birth body length (BBL), birth chest circumference (BCC), birth shoulder height (BSH), weaning body length (WBL), weaning chest circumference(WCC), weaning shoulder height (WSH) and average daily gain (ADG) were collected and used for analysis of MC4R gene. Sixty blood samples were collected for DNA isolation and PCR amplification. The single nucleotide polymorphisms (SNP) g.1133 C>G was used for genotyping by using PCR-RFLP methods. The frequenciy of G allele (0.59) was greater than C allele (0.41). The highest genotype frequencies have been detected in CG heterozygote animals (0.52) followed by GG (0.33) and CC (0.15) in homozygote animals. The results of Pearson ‘s Chi-square test indicated that the population was not deviate (P>0.05) from the Hardy-Weinberg equilibrium (HWE). The SNP g. 1133 C>G of MC4R gene indicated affecting high birth body length with GG genotype (P<0.05). In conclusion, the SNP g. 1133 C>G may can be a marker for birth body length of calf selection.

IDENTIFICATION OF MELANOCORTIN 1 RECEPTOR (MC1R) GENE BASED ON COAT COLOR OF BALI COWS OF KUPANG BY USINGTHE PCR-RFLP METHOD Tabun, A.C.; Hartatik, T.; Sumadi, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 2 (2013): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.2.86-91

The objective of this study was to identify the Melanocortin 1 Receptor (MC1R) gene in sorrel-,black- and white-Bali cows originated from Kupang-East Nusa Tenggara. Blood samples were takenfrom 46 cows consisted of of 17, 18 and 8 of sorrel, black and 8 heads, respectively, and 3 heads of bull.The PCR-RFLP method was performed to identify the gene. Amplification of PCR gene MC1R ofKupang's Bali Cattle (KBC) was 296 bp. The digestion by using MspI enzyme showed that there wereEE genotype (169, 136 bp) in Bali bull, sorrel and white cows. Two 2 genotypes, those were EE (169,136 bp) and Ee (296, 169, 136 bp) were found in black Bali cow. The frequency of E alele of KBC was0.99 (99%) and e alele was 0.01 (1%). MC1R gene in KBC was monomorphic and the it can be used asthe marker of the coat color in the population.

Association of Melanocortin 4 Receptor gene polymorphism with growth traits in Bligon goat Latifah, L.; Maharani, D.; Kustantinah, A.; Hartatik, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 4 (2018): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.4.343-351

Melanocortin 4 receptor gene is a potential marker gene for growth performance in ruminant and non-ruminant animals. The objective of this study was to perform allele and genotype frequencies, and association of melanocortin 4 receptor with growth traits in Bligon goat. Seventy seven blood samples were collected and DNA isolated successfully. One pair primers were used for PCR of MC4R gene (F: 5’-TCGGGCGTCTTGTTCATCAT-3’ and R: 5’-CAAGACTGGGCACTGCTTCA-3’). Two SNPs (g.998A/G and g.1079C/T) were identified in exon of Bligon MC4R by DNA sequencing and PCR-RFLP. The SNP g.998A/G identified amino acid change from isoleucine to methionine (I-M), while the SNP g.1079C/T was synonymous polymorphism. Based on relationship analysis between Genotype of MC4R gene (SNP g.998A/G) and growth traits indicated that GG genotype was lowest in weaning weight (WW; AA=13.0±1.7; AG=11.2±3.6; GG=9.2±3.9), weaning body length (WBL; AA=46.8±2.4; AG=46.3±6.0; GG=43.8±5.3) and weaning chest circumference (WCC; AA=50.9±4.0; AG=50.7±4.6; GG=47.1±7.3) compare to AA and AG genotype (p<0.05). Individual with CC and CT genotypes at SNP g.1079C/T had higher (P<0.05) in WW (CC=12.6±2.1; CT=12.5±2.5) and average daily gain (ADG; CC=81.2±21.8; CT=79.3±26.2) than TT genotype (WW=10.6±3.1; ADG=58.3±30.5). This results suggested that SNP g.998A/G and g.1079C/T of MC4R gene could be used as the genetic marker of growth traits for selection in Bligon goat.

Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle Hartatik, T.; Putra, D. E.; Volkandari, S. D.; Kanazawa, T.; Sumadi, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 1 (2018): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.1.1-8

Growth hormone gene that controls metabolism and body size of animals and polymorphism of the gene is known related to performance of livestock. Identification of gene polymorphisms was important to get early information to determine genetic markers associated with economically desirable traits. Genetic mapping is one of important characterizations in the selection process. Pesisir cattle is one of local cattle in Indonesia that are reared in West Sumatera and have become adaptive to local environment. The present study aimed to identify the genotype of Growth Hormone gene (GH891│MspI) of the Pesisir cattle and crossbred Simmental-Pesisir cattle (designated as SimPes) and relationship between growth performance (body weight and body size). The present study was conducted to 30 blood samples consisted of 15 Pesisir cattle and 15 SimPes cattle. Body weight and body size were measured at 12-18 months of age. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method was used in this research. The results showed that there exist three variant genotypes (MspI-/-, MspI+/-, MspI+/+) and that allele frequencies of MspI- and MspI+ were 53.3% and 46.7%, respectively. Non-significant effect has found between GH’s genotype and growth traits (Body measurement and body weight) in both of breed cattle. In conclusion, three variant genotypes (GH891│MspI) in Pesisir and SimPes (Simmental x Pesisir) cattle population were found and no significant effect on growth trait performance.

GROWTH HORMONE GENE GENOTYPING BY Msp I RESTRICTION ENZYME AND PCR-RFLP METHODS IN ACEH CATTLE BREED AT INDRAPURI DISTRICT OF ACEH PROVINCE Putra, W.P.B.; Hartatik, T.; Sumadi, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 4 (2013): (December)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.4.207-211

The objective of this research was to identify growth hormone (GH) genes genotype in selectedAceh cattle at Indrapuri’s Breeding and Forage Centre (IBFC) of Aceh Cattle. Fourty one cattleconsisting of 21 male and 20 female cattle were used in this study. The genomic DNA was extractedfrom blood using Sambrook et al. (1989) methods. Polymerase Chain Reaction - Restriction FragmentLength Polymorphism (PCR-RFLP) and mehod of sequencing was used to detect MspI site on GH gene.Based on sequencing results, it can be concluded that all cattle were monomorphism. The frequency ofTT genotype were 1.00 and same as T allele frequency. The transition of C (cytosine) into T (thymine)on 1548 bp caused the lost of restriction site.

MORPHOLOGY AND GENETIC DIVERSITY OF MITOCHONDRIAL DNA D-LOOP REGION USING PCR-RFLP ANALYSIS IN MAGELANG DUCK AND OTHER NATIVE DUCK Purwantini, D.; Yuwanta, T.; Hartatik, T.; Ismoyowati, I.
Journal of the Indonesian Tropical Animal Agriculture Vol 38, No 1 (2013): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.38.1.1-9

The aim of this study was to investigate the different of plumage colors on morphological diversityof Magelang duck and genetic diversity using PCR-RFLP mtDNA D-loop region analysis of Magelangduck and four others native duck population (Tegal, Mojosari, Bali and Alabio duck) in Indonesia. Bloodsample was taken from 50 Magelang ducks and 20 of each native ducks. Morphological characteristicsof body measurement, production ability and egg quality of Magelang duck were analyzed usingCompletely Randomized Design with 11 plumage colors as treatments. PCR technique was administeredto amplify fragments in mtDNA D-loop region and PCR products were digested with endonucleaserestriction enzyme AluI and HaeIII. The result showed that morphology diversity of Magelang duck wasstatistically affected by different plumage colors. PCR-RFLP analysis using AluI and HaeIII restrictionenzyme resulted in six combinations of restriction fragment pattern shown in six haplotypes (A, B, C, D,E and F). Haplotype difference showed genetic diversity in the population of Magelang duck and theother native ducks. In conclusion, the different plumage colors affected morphology diversity ofMagelang duck. Genetic diversity of Indonesian native duck population could be identified by usingPCR-RFLP analysis on mtDNA D-loop region.

ASSOCIATIONS BETWEEN POLYMORPHISM OF GROWTH HORMONE GENE WITH MILK PRODUCTION, FAT AND PROTEIN CONTENT IN FRIESIAN HOLSTEIN CATTLE Hartatik, T.; Kurniawati, D.; Adiarto, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 3 (2015): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.3.133-137

The aim of the research was to determine the associations between polymorphism of the bovine growth hormone (GH) gene (Leu/Val) and milk production of Friesian Holstein Cattle. A total of 62 cows which consist of two Friesian Holstein cattle groups (from New Zealand=19 heads and Australia=43 heads) were used for the research. We performed the Polymerase Chain Reaction and followed by Restriction Fragment Length Polymorphism using AluI restriction enzyme. The genotype was observed base on the target gene GH 211 bp in the part of intron 4 and exon 5 of growth hormone gene. The frequencies of genotypes LL were found higher than genotype LV in both groups. Friesian Holstein cattle from New Zealand showed the genotype LL and LV as 84% and 16%, respectively. Friesian Holstein cattle from Australia show the genotype LL and LV as 79% and 21%, respectively. The association between Leu/Val polymorphism on milk production, fat and protein content in both groups did not show the significant effect. Base on two groups of the origin of cattle, the result showed the significant different on fat and protein content of milk. Fat and protein contents of milk were higher in breed of FH imported from Australia compared to those in breed of FH imported from New Zailand.

Association of IGFBP-3 gene polymorphism g. 3.930 G>A with birth size and birth weight in crossbred beef cattle Hartatik, T.; Priyadi, D. A.; Panjono, P.; Bintara, S.; Ismaya, I.; Budisatria, I. G. S.; Widyobroto, B. P.; Agus, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.356-363

Crossing between cattle breeds is the one way to improve the genetic quality of cattle. Controlling crosses using genetic markers will speed up the process. The IGFBP-3 gene is a potential marker of selection due to its role in regulating growth and metabolism in cattle. The aim of this study was to determine the effect of genotype on birth size and birth weight using IBFBP-3 gene marker. A total of 32 cattle consisted of 10 Brahman Cross cattle, 14 cattle results of Brahman Cross cow crosses with Belgian Blue bull, and 8 cattle results of Brahman Cross cow crosses with Wagyu bull. Sequences of 563 bp, including the part intron 2, exon 3, and the part intron 3. Genotyping was done by sequencing method and PCR-RFLP method with the restriction enzyme PvuII. An SNP was identified at location g 3,930 (G>A). Two genotypes were observed, namely GA and GG. The genotype did not affect the performance of birth (body weight, body length, height at withers, heart girth) of the research sample. Birth traits are influenced by the cattle breed, it can be seen that Brahman Cross cattle have a body length that is bigger than the other crossbred.

Polymorphism of MC4R gene associated with feed intake, nutrient digestibility, ADG and FCR at post-weaning in Bligon goats Latifah, L.; Maharani, D.; Kustantinah, K.; Hartatik, T.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 3 (2020): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.3.173-180

The melanocortin-4 receptor (MC4R) gene plays an important role in sympathetic nerve activity, as well as adrenal and thyroid function and mediates the effects of leptin on energy homeostasis. This study aimed to investigate the effect of the 642 bpMC4R gene on feed intake, nutrient digestibility, average daily gain (ADG),and feed conversion ratio (FCR)at post-weaned in Bligon goat. Forty-six Bligon were used for the blood sample collection, and genomic DNA was extracted using the Geneaid isolation kit. Feed intake data were collected on 46 Bligon goats kept on 2 different animal houses, including the laboratory’s farm of Faculty of Animal Science Universitas Gadjah Mada (FAS UGM) with n=23 and Banyusoco farm (n=23). Variables observed were feed intake, nutrient digestibility,ADG,and FCR. All samples were genotyped using the PCR-RFLP method with the KpnIenzyme. One SNP was located in the exonic region (g.1079C/T). The t-test analysis revealed a significant association between SNP g.1079C/T and the digestibility of dry matter (DM), organic matter (OM),and total digestible nutrient (TDN) of Bligon goats at 7 months of age kept on FAS UGM, as well as the digestibility of DM, crude protein (CP), nitrogen-free extract (NFE),and TDN of Bligon goats at 7 months of age kept on Banyusoco. In conclusion, it is possible to use SNP g.1079C/T for animal genotyping and asaMAS for nutrient digestibility in Bligon goats

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