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Carcass and physical meat characteristics of thin tail sheep (TTS) based on calpastatin gene (CAST) (Locus intron 5 â exon 6) genotypes variation Dagong, Muhammad Ihsan Andi; Herman, R; Sumantri, C; Noor, R.R; Yamin, M
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 1 (2012)
Publisher : Indonesian Animal Sciences Society

The quality of sheep carcass is mostly determined by the total lean meat production, meat distribution on the carcass and the quality of meat. Calpastatin gene (CAST) is known to have an association with carcass and meat quality traits. The objective of this research was to identify the association between CAST polymorphisms and carcass characteristics in Thin Tail Sheep (TTS). Thirty three heads of sheep representing three genotypes of CAST (CAST-11, CAST-12 and CAST-22) were identified for carcass and meat characterisation. There was no significant difference between CAST polymorphisms with meat tenderness, pH, water holding capacity and cooking loss, neither with carcass weight and dressing percentage among genotypes. Shoulder proportion of CAST-11 genotype was larger than that of CAST-12 or CAST-22, but the lean meat proportion of CAST-22 genotype in shoulder, rack and loin were higher than those of CAST-11 but not different from CAST-12. The fat percentage of CAST-11 was the highest among the genotypes. CAST-22 genotype has higher lean meat percentage than the CAST-11. Variation in CAST gene could be used as marker assisted selection in sheep for higher lean meat proportion. Key Words: Calpastatin, Polymorphisms, Carcass Characteristics, Meat Quality, Thin Tail Sheep

Tingkah Laku Makan Kambing Lokal Persilangan yang Digembalakan di Lahan Gambut: Studi Kasus di Kalampangan, Palangkaraya, Kalimantan Tengah R Setianah; S Jayadi; R Herman
Media Peternakan Vol. 27 No. 3 (2004): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Central Kalimantan is one of the province passed by equator line. The temperature is relatively hot, during the day time is 32 0C and 23 0C during night time. The average rainfall index is 1900-3100 mm per year. This province has remarkably wide peatland area with strong acidity, high organic matter, and low fertility for plant cultivation. Various existing vegetation can be used as feed. Goats are able to utilize many type of grasses, leaves and tree bark. They have high ability to adapt various environments and eat many type of plants. Due to their browsing ability, goats can utilize tall bushes. The objective of this experiment was to study grazing behaviour of Crossed Local goats. The Pattern of grazing behaviour of goats can be used as a basis for managing animals and range land on the peatland areas. The experiment used 5 male goats aged 8-12 months and 5 females aged 10-24 months. Recording methode used One Zero with 15 minutes intervals. Data were analysed using Comparison of Two Samples or t-Test (t student) at level 5%. Result of research indicated that the goat activity in day time (09.00-16.00) was dominated by grazing activity (male 66,28%, female 60,82%). The goats spent more time for eating in the morning and evening (09.00-10.00 and 13.00-16.00). Grazing rumination and resting activities during investigation between male and female were not significantly different. Browsing is the most activity observed compared to other activities. Crop types are diverse in peatland areas. The result show that sasendok vegetation (Plantago mayor), Delingu (Dianella ensifolia sp.) and Kelakai (Stenochlaena palustris) were the most preferred vegetation by the goats on the peatland areas. Key words: grazing behaviour, Crossed Local goats, peatland

Produksi Karkas dan Non Karkas Domba Priangan dan Ekor Gemuk pada Bobot Potong 17,5 dan 25,0 Kg R Herman
Media Peternakan Vol. 28 No. 1 (2005): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

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Carcass and non-carcass production of Priangan and Fat Tail sheep were studied at the Department of Animal Production, Faculty of Animal Science, Bogor Agricultural University. Six male Priangan and six male Fat Tailed lambs were killed at 17.5 kg slaughter weight. A similar number of both breeds were also killed at 25.0 kg. Priangan and Fat Tail of 18.483 ± 0,160 and of 18.583 ± 0.262 kg body weight were fasted 24 hours (water was allowed) and fasted body weight (slaughter weight) were 17.600 ± 0.089 and 17.490 ± 0.020 kg, respectively. Similarly, Priangan and Fat Tail of 26.190 ± 0.207 and 26.283 ± 0.349 kg were also fasted at the same treatment and slaughter weights were 24.914 + 0.183 and 24.890 ± 0.114 kg. Before killing, they were reared on a pelleted ration containing 73.3% TDN and 16% crude protein (100% dry matter basis). Those animals were killed and dissected. The results showed that there were no significant differences in the weight of carcass, organs and offals, except in the weight of head and tail at 17.5 kg (P < 0.05) and at 25.0 kg (P < 0.01). At 17,5 kg slaughter weight, Priangan (17600 ± 89 g) produced 47.10% carcass, 11.14% head and shank, 6.67% skin, 14.38% offals, 4.84% blood and 12.71% gut content; Fat Tail (17490 ± 20 g) produced 48.76% carcass, 9.20% head and shank, 7.00% skin, 13.38% offals, 4.08% blood and 11.99% gut content. At 25.0 kg slaughter weight, Priangan (24917 ± 183 g) produced 48.70% carcass, 10.14% head and shank, 6.90% skin, 12.90 offals, 5.03% blood and 12.12% gut content. Fat Tail produced 52.39% carcass, 8.39% head and shank, 7.00% skin, 13.11% offals, 4.19% blood and 10.61% gut content. Key words: Priangan sheep, carcass

Genetic Polymorphisms of the Coding Region (Exon 6) of Calpastatin in Indonesian Sheep M I.A Dagong; C Sumantri; R R Noor; R Herman; M Yamin
Media Peternakan Vol. 34 No. 3 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Calpastatin (CAST) is an indigenous inhibitor of calpain that involved in regulation of protein turn over and growth. The objective of this research was to identify genetic polymorphisms in the entire exon 6 of calpastatin gene in Indonesian local sheep. A PCR-SSCP method was carried out to identify genetic variation of CAST gene. In total 258 heads of local sheep from 8 populations were investigated, three groups of samples were Thin Tail Sheep (TTS) from Sukabumi, Jonggol, and Kissar. The rest samples were Priangan sheep (PS) from Margawati (Garut meat type) and Wanaraja (Garut fighting type) and Fat Tail Sheep (FTS) from Donggala, Sumbawa, and Rote islands. SSCP analysis revealed that three different SSCP patterns corresponded to three different alleles in the CAST locus (CAST-1, 2, and 3 allele) with five different genotypes. Genetic variation between local sheep populations were calculated based on genotypic and allelic frequencies. Most populations studied were polymorphic, with genotype frequencies of CAST-11, CAST-12, CAST-22, CAST-32, and CAST-33 were 0.286, 0.395, 0.263, 0.046, and 0.007 respectively. CAST-1 and 2 alleles were most commonly found in all populations with total frequency was 0.970, while CAST-3 was a rare allele 0.030 and only found in TTS population. Variation in the CAST gene could be used for the next research as genetic diversity study or to find any association between CAST polymorphism with birth weight, growth trait and carcass quality in Indonesian local sheep.

Carcass and Non-carcass Components of Priangan and Javanese Fat-tailed Rams Slaughtered at Mature Live Weight M Baihaqi; R Herman
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Twenty-three of indigenous Priangan and Javanese Fat-tailed (JFT) ram breeds were used to evaluate its carcass and non carcass components slaughtered at mature live weight. Five Priangan rams and six JFT rams were slaughtered at 32.5 kg of live weight while six other rams of each breeds were also slaughtered at 40 kg of live weight. Before the rams were slaughtered, they werefattened using pellet ration which was formulated according to NRC for fattening 10 kg of lamb, containing 73.3% TDN and 16% protein in dry matter bases. Feed and water were given ad libitum. Rams were slaughtered and dissected when they reached their assigned slaughter weight (32.5 and 40 kg). The results showed that both breeds had high percentage of carcass (53%-55%) with no significantly different between breeds (P > 0.05) either were slaughtered at 32.5 kg or 40 kg. However, carcass of Priangan rams had significantly more muscle, but less fat than Javanese Fat-tailed rams at mature live weight (P < 0.05). Non-carcass components were not significantly different between breeds (P > 0.05) except for head, tail and testes (P < 0.01) and liver (P < 0.05). The edible and inedible portion of non-carcass rangedfrom 30%-32% and 12%-15% of slaughter weight, respectively.

Carcass and physical meat characteristics of thin tail sheep (TTS) based on calpastatin gene (CAST) (Locus intron 5 – exon 6) genotypes variation Muhammad Ihsan Andi Dagong; R Herman; C Sumantri; R.R Noor; M Yamin
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The quality of sheep carcass is mostly determined by the total lean meat production, meat distribution on the carcass and the quality of meat. Calpastatin gene (CAST) is known to have an association with carcass and meat quality traits. The objective of this research was to identify the association between CAST polymorphisms and carcass characteristics in Thin Tail Sheep (TTS). Thirty three heads of sheep representing three genotypes of CAST (CAST-11, CAST-12 and CAST-22) were identified for carcass and meat characterisation. There was no significant difference between CAST polymorphisms with meat tenderness, pH, water holding capacity and cooking loss, neither with carcass weight and dressing percentage among genotypes. Shoulder proportion of CAST-11 genotype was larger than that of CAST-12 or CAST-22, but the lean meat proportion of CAST-22 genotype in shoulder, rack and loin were higher than those of CAST-11 but not different from CAST-12. The fat percentage of CAST-11 was the highest among the genotypes. CAST-22 genotype has higher lean meat percentage than the CAST-11. Variation in CAST gene could be used as marker assisted selection in sheep for higher lean meat proportion. Key Words: Calpastatin, Polymorphisms, Carcass Characteristics, Meat Quality, Thin Tail Sheep

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