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All Journal Current Biochemistry Journal Biodidaktika : Jurnal Biologi dan Pembelajarannya
Fachriyan Hasmi Pasaribu
Institut Pertanian Bogor
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences Other

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Antimicrobial Activity of Allium chinense G. Don. Frans Grovy Naibaho; Maria Bintang; Fachriyan Hasmi Pasaribu
Current Biochemistry Vol. 2 No. 3 (2015)
Publisher : IPB University

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Abstract

This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-mass­spectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.
Aktivitas Antibakteri Isolat Bakteri Endofit dari Tanaman Miana (Coleus scutellariodes [L.] Benth.) terhadap Staphylococcus aureus dan Escherichia coli Dwi Endah Kusumawati; Fachriyan Hasmi Pasaribu; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Abstract

Coleus scutellarioides [L.] Benth. known as medicinal plant which contains antidiarhea and antimicrobial compounds. Extraction of bioactive compound from plants is not efficient because it needs a large biomass, therefore the one of appropiate ways is using endophytic bacteria. The purpose of this research was to isolate and test of antibacterial activity of endophytic bacteria from Coleus scutellarioides [L.] Benth. against two pathogenic bacteria i.e Eschericia coli and Staphylococcus aureus. A total of 22 isolates of endophytic bacteria were obtained from Coleus scutellarioides [L.] Benth. Based on antibacterial activity test, 13 isolates of endophytic bacteria were able to inhibit the growth of E. coli and 15 isolates were able to inhibit S. aureus, whereas endophytic bacteria which can inhibit both types of pathogens are 10 isolates.
Isolasi Bakteri Endofit dari Tanaman Sirih Hijau (Piper betle L.) dan Potensinya sebagai Penghasil Senyawa Antibakteri Ukhradiya Magharaniq Safira; Fachriyan Hasmi Pasaribu; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Abstract

Endophytic bacteria are beneficial microorganisms that interact with host plants without causing disruption or damage to the host. Some studies suggest that certain endophytic bacteria can produce chemical compounds that have an effect on health, especially endophytic bacteria isolated from medicinal plants. Green betel (Piper betle L.) is a medicinal plant that has been used for years and has many benefits. The purposes of this study are to isolating and screening of endophytic bacteria from green betel against four pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Bacillus cereus and Salmonella enteritidis). The number of endophytic bacteria that has been isolated are 14 isolates. Based on the screening results, three isolates of endophytic bacteria have potential activity (characterized by the formation of inhibition zone) against S. aureus. The inhibition zone may indicate that those isolates produce compounds that have antibacterial effects. Those isolates are AS1, BS1 and BS2. The biggest inhibition zone showed by BS1, so it can be concluded that BS1 is the most potential isolate as a novel source of antibacterial compound.
PENGEMBANGAN UJICEPATMETODE KOAGLUTINASI UNTUK MENDETEKSI ANTIGEN VIBRIO PARAHAEMOLYTICUS PENYEBAB PENYAKIT VIBRIOSIS PADA UDANG VANAME(Litopenaeus vannamei) Yan Evan; Agustin Indrawati; Fachriyan Hasmi Pasaribu
Biodidaktika : Jurnal Biologi dan Pembelajarannya Vol 16, No 1 (2021)
Publisher : Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30870/biodidaktika.v16i1.10784

Abstract

Vibrio parahaemolyticus merupakan salah satu agen penyebab penyakit vibriosis pada udang vaname (Litopenaeus vannamei. Untuk penanganan lebih dini serta mencegah tersebar luasnya penyakit ini, diperlukan metode uji yang cepat dan akurat. Tujuan penelitian adalah untuk membuatkit ujikoaglutinasi yang dapat mendeteksi antigen bakteriV. parahaemolyticus. Antibodi poliklonal V. parahaemolyticus yang digunakan merupakan hasil imunisasi pada kelinci yang disuntikan antigen V. parahaemolyticusdengan dosis 3×108cfu/ml sebanyak 1 mL. Penyuntikan antigen dilakukan sebanyak empat kali pada interval satu minggu melalui vena auricularis.Reagen koaglutinasi dibuat dengan cara melakukan pencampuran antara suspensiS. aureus yang memiliki protein A dan serum IgG V. parahaemolyticushasil purifikasi dengan perbandingan 1:1 (v/v).Reagen koaglutinasi ini selanjutnya dipergunakan untuk pengujian terhadap sampel organ hepatopankreas, usus dan daging udang yang terinfeksi bakteri V. parahaemolyticus dan bakteri lain yaitu V. harveyi dan V. alginolyticus untuk pengujian reaksi silang.Hasil pengujian reaksi koaglutinasi positif hanya terjadi pada sampel organ yang terinfeksi bakteri V. parahaemolyticus, ini ditandai dengan terbentuknya aglutinat. Sedangkan hasil pengujian reaksi silang dengan menggunakan sampel organ yang terinfeksi bakteri V. harveyi dan V. alginolyticus menunjukkan hasil negatif, ditandai dengan suspensi tetap homogen tidak terbentuk aglutinat. Berdasarkan hasil pengujian koaglutinasi membuktikan bahwa reaksi reagen koaglutinasi bersifat spesifik, cepat dan akurat.
Co-Authors Agustin Indrawati Dwi Endah Kusumawati Frans Grovy Naibaho Maria Bintang Purwanto, Ukhradiya Magharaniq Safira Yan Evan