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All Journal Current Biochemistry Journal JURNAL ILMU KEFARMASIAN INDONESIA
Maria Bintang
Institut Pertanian Bogor
Biochemistry, Genetics & Molecular Biology Education Medicine & Pharmacology

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In Silico Analysis of Curcuma longa Against PCAF Histon Asetiltransferase Artha Vinsentricia; Djarot Sasongko Hami Seno; Maria Bintang
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Abstract

Curcumin turmeric (C. longa) has been found capable of inhibiting p300/CBP associated factor (PCAF) histone asetyltransferase (HAT) as a cancer biomarker. However, the molecular mechanism of inhibition is unkown. This study is aimed to analyse the molecular inhibition mechanisms by in silico method. The analysis showed that curcumin’s Gibbs free energy (ΔG) was stable and capable of binding amino acid residues believed to be active site of PCAF HAT. Curcumin is predicted to be a PCAF HAT inhibitor, and further in vitro analysis is required.
Immobilization of Rhizopus oryzae Lipase on Zeolit, CaCO3, Silica Gel, and Cow Bone Maria Bintang; Tri Panji; Susi Saadah
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Food production in Indonesia is constrained by the high cost of lipase that is still imported from abroad. To overcome this problem, research of food production has been conducted using crude extract of lipase produced by indigenous species of fungi Rhizopus oryzae. The R. oryzae is edible indicating that it is safe to be used in the production of food products. Enzymes have an ability to catalyze specific chemical reactions with high efficiency and low energy cost. Enzyme immobilization is a recovery techique that has been studied in several years, using supporting materials as a medium to help enzyme dissolutions to the substrate. Several supporting materials such as zeolit, CaCO3, silica gel, and cow bone were selected by its ability to adsorb lipase. CaCO3 shows enzyme loading rate respectively 99.46%, giving more lipase to adsorb than zeolit (90.69%), cow bone (91.56%), and silica gel (59.63%). In this research, condition factors, such as optimum pH, optimum temperature, and storage ability of the matrix were investigated. Free lipase reacts optimally at pH 7 and temperature 30°C. Identical result showed for lipase in cow bone. Lipase in CaCO3 reacts optimally at pH 8 and temperature 35°C. Lipase in zeolit and silica gel reacts optimally at pH 8 and temperature 30°C. Half life time estimation was one week in storage condition temperature at 4°C and each optimum pH.
Isolation and Characterization of Endophytic Bacteria from Tembelekan (Lantana camara L.) as Antibacterial Compounds Producer Dina Dyah Saputri; Maria Bintang; Fachriyan H Pasaribu
Current Biochemistry Vol. 2 No. 2 (2015)
Publisher : IPB University

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Endophytic bacteria are microorganisms that live in the internal tissues of plants and have symbiotic mutualism with their host plants. Endophytic bacteria may produce secondary metabolites that can be developed for medical, agricultural, and industrial purposes. Lantana camara is a medicinal plant that has therapeutic potential to treat a variety of diseases such as fever, tuberculosis, rheumatism, asthma, and skin disease. The purpose of this study was to isolate and characterize endophytic bacteria from Lantana camara which has potential to produce antibacterial compounds. The method of this research include isolation of endophytic bacteria of Lantana camara. Antibacterial activity assay was done against four types of pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus, and Salmonella enteritidis. Characterization of endophytic bacteria was by 16S rRNA gene analysis and identification of antibacterial compounds by GC-MS analysis. Isolation of endophytic bacteria from Lantana camara resulted in BT22 as a potential isolate. Analysis of 16S rRNA gene showed that the BT22 isolate was similar to Bacillus amyloliquefaciens YB-1402 with 99% identity. The results of GC-MS analysis showed some antibacterial compounds such as: Cyclohexanone, 2-[2-(1,3-dithiolan-2-yl)propyl]-6-methyl-3-(1-methylethyl), Octadecane (CAS) n-Octadecane and Tetracosane (CAS) n-Tetracosane.
Kandungan Fitokimia, Total Fenol, dan Total Flavonoid Ekstrak Buah Harendong (Melastoma affine D. Don) Novilia Eka Syafitri; Maria Bintang; Syamsul Falah
Current Biochemistry Vol. 1 No. 3 (2014)
Publisher : IPB University

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Melastoma affine plant has effect on health such as curing wound and toothache, also as an antimalarial drug. The fruit of this plant is purple and probably contain secondary metabolite compounds such as phenols and flavonoids. The total amount of both compounds may be different in unripe and ripe fruit. The aims of this research were to analyze secondary metabolite compounds and determine total phenol and total flavonoid of Melastoma affine fruit extract. The samples used in this study were unripe and ripe Melastoma affine fruits. Both of samples were extracted by three different solvents (water, 70% ethanol, and 96% ethanol) and obtained 6 extracts. Based on phytochemical test, each extract from unripe and ripe Melastoma affine fruit contained alkaloid, triterpenoid, flavonoid, phenol, and tanin. The extract with highest total phenols was 70% ethanolic extract from unripe fruit (189.56 mg/g GAE), while the extract with highest total flavonoids was 96% ethanolic extract from unripe fruit (225.50 mg/g CE). Based on this result, we conclude that unripe fruit of Melastoma affine has more total phenols and total flavonoids than ripe fruit.
Antimicrobial Activity of Allium chinense G. Don. Frans Grovy Naibaho; Maria Bintang; Fachriyan Hasmi Pasaribu
Current Biochemistry Vol. 2 No. 3 (2015)
Publisher : IPB University

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This study aims to analyze antimicrobial activity of Allium chinense G. Don extract against Eschericia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis and Candida albicans, and to examine the active compounds. Allium chinense G. Don was extracted using maseration method and treated with ethanol 70% (v/v), ethanol 96% (v/v), ethyl acetate, n-hexane, and aquadest. Antimicrobial activity assay was conducted using agar difusion method and compounds analysis using Gas chromatography-mass­spectrometry (GC-MS). Antimicrobial activities assay showed that all extracts could inhibit microbial growth. Ethyl acetate extract has the highest antimicrobial activity against all the microbial test. Minimum inhibitory concentration (MIC) of ethyl acetate extract against C. albicans, B. subtilis, E. coli, S. aureus, S. typhi was 25, 100, 250, 250, 1000 mg ml-1 respectively. As many as 25 compounds were derived from GC-MS analysis and most of them were known as the antimicrobial compounds. This study revealed that Allium chinense G. Don contains biologically active compunds as antimicrobial agent particularly anti Candida.
Induksi dan Karakter Pertumbuhan Kalus Triploid dari Endosperma Avokad (Persea americana Mill.) Edy Sukmara; Lazarus Agus Sukamto; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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The concentration of the Plant Growth Regulators (PGR) medium as well as endosperm viability determines the success of triploid avocado callus. This study was aimed to explore the best size of diameter fruit and the most responsive concentration for the induction of endosperm avocado callus. The study consisted of two phases. The first stage is to determine the best diameter size of avocado and concentration of the culture medium that gave the fastest response to be endosperm callus. The second stage used flow cytometer to obtain triploid of avocado endosperm callus. This study used four size of fruit group diameter, that A=(0.30-0.50), B=(0.51-1.00), C=(1.01-1.50) and D=(1.51-2.20) in cm. Factorial experiment with four replications was arranged in a completely randomized design. Murashige and Skoog medium (MS) was used in this culture with two PGR that the Picloram and 2.4-D. Each with concentration : 0.5, 1.0, 2.0 and 4.0 in mg/l. The most responsive diameter fruit size was group B (0.30 – 0.50 cm) with the fastest callus growth average was 7.67 weeks after culture (WAC). The most responsive concentration for endosperm callus induction was Picloram 2 mg/l, growth in 5.08 WAC. The endosperm callus growth successfully t done by the inclusion of avocados embryos. The best interaction between fruits diameter and concentration for callus avocado induction used fruits diameter A with picloram 2 mg/l. The endosperm callus were measured by using flow cytometer resulted in avocado triploid callus with region range (RN) Mean value wer 302.01, 296.60, and 298.51 respectively. While the avocado leaf control resulted in 198.82 diploid plant.
Aktivitas Antibakteri Isolat Bakteri Endofit dari Tanaman Miana (Coleus scutellariodes [L.] Benth.) terhadap Staphylococcus aureus dan Escherichia coli Dwi Endah Kusumawati; Fachriyan Hasmi Pasaribu; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Coleus scutellarioides [L.] Benth. known as medicinal plant which contains antidiarhea and antimicrobial compounds. Extraction of bioactive compound from plants is not efficient because it needs a large biomass, therefore the one of appropiate ways is using endophytic bacteria. The purpose of this research was to isolate and test of antibacterial activity of endophytic bacteria from Coleus scutellarioides [L.] Benth. against two pathogenic bacteria i.e Eschericia coli and Staphylococcus aureus. A total of 22 isolates of endophytic bacteria were obtained from Coleus scutellarioides [L.] Benth. Based on antibacterial activity test, 13 isolates of endophytic bacteria were able to inhibit the growth of E. coli and 15 isolates were able to inhibit S. aureus, whereas endophytic bacteria which can inhibit both types of pathogens are 10 isolates.
Delignifikasi Batang Kayu Sengon oleh Trametes versicolor Azmi Azhari; Syamsul Falah; Laita Nurjannah; Suryani Suryani; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Delignification is a lignin degradation, a preliminary process in industries that used cellulose containing substrates. Sengon logs are often used for the material in pulp industry because it has high levels of cellulose and low level of lignin. The aim of this study was delignification of sengon logs by using T.versicolor. The methods used include observation growth of T.versicolor compared with Phanerochaete chrysosporium, the rate of of lignin degradation (black liquor), delignification of sengon logs using T.versicolor and the chemical assay of sengon logs before and after delignification. The results of this study showed that delignification by T.versicolor was faster compared to P.chrysosporium based on the rate of lignin degradation (black liquor). The result showed that delignification by T.versicolor at room temperature reduced lignin of sengon logs by 37.31% within 20 days. Chemical assay performed on delignified sengon wood showed decreased level of ethanol benzene, soluble extractive substances, holocellulose, and cellulose and an increase of hemicellulose level.
Isolasi Bakteri Endofit dari Tanaman Sirih Hijau (Piper betle L.) dan Potensinya sebagai Penghasil Senyawa Antibakteri Ukhradiya Magharaniq Safira; Fachriyan Hasmi Pasaribu; Maria Bintang
Current Biochemistry Vol. 1 No. 1 (2014)
Publisher : IPB University

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Endophytic bacteria are beneficial microorganisms that interact with host plants without causing disruption or damage to the host. Some studies suggest that certain endophytic bacteria can produce chemical compounds that have an effect on health, especially endophytic bacteria isolated from medicinal plants. Green betel (Piper betle L.) is a medicinal plant that has been used for years and has many benefits. The purposes of this study are to isolating and screening of endophytic bacteria from green betel against four pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Bacillus cereus and Salmonella enteritidis). The number of endophytic bacteria that has been isolated are 14 isolates. Based on the screening results, three isolates of endophytic bacteria have potential activity (characterized by the formation of inhibition zone) against S. aureus. The inhibition zone may indicate that those isolates produce compounds that have antibacterial effects. Those isolates are AS1, BS1 and BS2. The biggest inhibition zone showed by BS1, so it can be concluded that BS1 is the most potential isolate as a novel source of antibacterial compound.
Identifikasi Lektin Umbi dari Typhonium flagelliforme (Lodd.) Blume MUHAMMAD ALFARABI; SISWA SETYAHADI; MARIA BINTANG; MIFTAHUDIN MIFTAHUDIN; CHAIDIR CHAIDIR
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 1 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Typhonium flagelliforme atau yang dikenal dengan keladi tikus merupakan tumbuhan perdu yang banyak tumbuh di Indonesia. Tumbuhan ini mengandung lektin pada umbinya yang memiliki aktivitas antikanker. Namun hingga saat ini, tidak ada informasi ilmiah yang mengkaji mengenai lektin yang terdapat di umbi T. flagelliforme yang tumbuh diberbagai wilayah Indonesia. Tujuan dari penelitian ini adalah untuk mengidentifikasi dan mengkarakterisasi lektin umbi dari tujuh aksesi T. flagelliforme di Indonesia. Umbi yang digunakan dalam penelitian berumur 6 bulan tanam. Ekstrak protein umbi yang didapat dianalisis kandungan protein, toksisitas, dan aktivitas hemaglutinasinya. Aksesi yang memiliki aktivitas hemaglutinasi tertinggi, lektinnya dipurifikasi menggunakan kolom DEAE-Sepharose dan CM monolitic. Hasil menunjukkan bahwa protein umbi dari setiap aksesi memiliki toksisitas dan aktivitas hemaglutinasi. Aksesi Solok memiliki total protein dan aktivitas hemaglutinasi tertinggi dibanding aksesi lainnya. Aksesi ini memiliki kandungan lektin dengan bobot molekul 12,67 kDa. Lektin ini stabil pada temperatur 20 hingga 40 °C dan pada pH 5,0 hingga 7,2. Informasi karaktersitik ini dapat membantu dalam pengembangan obat antikanker berbasis lektin.
Co-Authors Artha Vinsentricia Azmi Azhari CHAIDIR CHAIDIR Dina Dyah Saputri Djarot Sasongko Hami Seno DJIHAN RYN PRATIWI Dwi Endah Kusumawati Edy Sukmara Fachriyan H Pasaribu Fachriyan Hasmi Pasaribu Frans Grovy Naibaho Laita Nurjannah Lazarus Agus Sukamto Miftahudin . Muhammad Alfarabi Novilia Eka Syafitri Purwanto, Ukhradiya Magharaniq Safira Simanjuntak, Partomuan SISWA SETYAHADI Suryani Suryani Susi Saadah Syamsul Falah Tri Panji