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KUALITAS SEMEN CAIR SAPI BALI SELAMA PENYIMPANAN SUHU RUANG MENGGUNAKAN PENGENCER SKIM MILK DENGAN PENAMBAHAN FILTRAT KECAMBAH KACANG HIJAU Zenni Nur Fadilah; Nurul Isnaini; Muhammad Nur Ihsan
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 1 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Penelitian ini bertujuan untuk mengetahui kualitas semen cair sapi Bali yang menggunakan pengencer skim milk dengan penambahan filtrat kecambah kacang hijau selama penyimpanan di suhu ruang (29oC). Hasil penelitian diharapkan dapat menjadi kajian ilmiah serta sumber informasi bagi akademisi dan Balai Inseminasi Buatan. Materi penelitian yang digunakan adalah semen segar sapi Bali yang dipelihara secara intensif di BBIB Singosari Malang dengan kriteria umur 6-11 tahun, motilitas massa ++ dan motilitas individu 50-70%. Metode penelitian yang digunakan adalah percobaan laboratorium menggunakan pola Rancangan Acak Kelompok (RAK) dengan 4 perlakuan dan 5 kali ulangan, P0 sebagai kontrol (100% Skim Milk + 0% Filtrat Kecambah Kacang hijau); P1 (98% SM + 2% FKKH); P2 (96% SM + 4% FKKH) dan P3 (94% SM + 6% FKKH). Analisis data yang yang digunakan adalah Analisis Ragam atau Analysis of Variant (ANOVA), apabila terdapat perbedaan yang nyata atau sangat nyata maka dilakukan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa penambahan filtrat kecambah kacang hijau ke dalam pengencer skim milk memberikan perbedaan yang tidak nyata (P>0,05) terhadap kualitas semen cair sapi Bali selama penyimpanan suhu ruang (29oC) baik motilitas individu, viabilitas maupun abnormalitas spermatozoa. Kata kunci: antioksidan, motilitas, viabilitas, abnormalitas

KUALITAS SEMEN SEGAR DAN PRODUKSI SEMEN BEKU SAPI SIMMENTAL PADA UMUR YANG BERBEDA Annisa Nyuwita; Trinil Susilawati; Nurul Isnaini
TERNAK TROPIKA Journal of Tropical Animal Production Vol 16, No 1 (2015): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Penelitian dilakukan di Balai Inseminasi Buatan Daerah Ungaran Jawa Tengah. Tujuan penelitian untuk mengetahui perbedaan kualitas semen segar dan produksi semen beku sapi Simmental pada umur yang berbeda. Materi yang digunakan dalam penelitian ini adalah catatan produksi semen yang terdiri dari kualitas semen segar dan jumlah straw semen beku dari 8 ekor sapi Simmental. Metode yang digunakan adalah studi kasus dengan Nested Design. Variabel yang diamati meliputi volume semen, pH, motilitas massa, persentase motilitas individu, konsentrasi spermatozoa, total spermatozoa, total spermatozoa motil dan jumlah straw semen beku. Hasil penelitian menunjukkanbahwa kualitas dan kuantitas semen segar pada 4 kelompok umur 3, 4, 7 dan 8 tahun secara berturut-turut adalah volume: 6,9±1,7; 7,3±0,5; 7,8±0,3 dan 9,2±1,6 ml, pH: 6,4±0,1; 6,5±0,1; 6,5±0,1 dan 6,5±0,1, motilitas massa: 2±0,04; 2±0; 2±0,05 dan 1,8±0,22 (+), persentase motilitas individu: 72.1±1.2; 71.3±0.8; 70.3±0.5 dan 66.3±5%, konsentrasi spermatozoa: 1.703,3±167,2; 1.612,2±172,2; 1.450,7±172,2 dan 1.254,6±113,6 (juta/ml), total spermatozoa: 11.586,5±1945,6; 10.468,1±1148,8; 12.593,6±947,8 dan 11.570,2±2642,2 juta sel, total spermatozoa motil: 8.341,8±1.282,5; 7.471,7±845,6; 8.857,6±662,7 dan 7.820,3±2.229,4 juta sel, jumlah straw semen beku: 300±38,2; 263,6±57,8 dan 236,6±180,1 buah straw. Kata kunci: semen beku, motilitas, konsentrasi, spermatozoa motil

PENGARUH PENGGANTIAN BOVINE SERUM ALBUMIN (BSA) DENGAN PUTIH TELUR DALAM PENGENCER DASAR Cep-2 TERHADAP KUALITAS SEMEN KAMBING BOER PADA SIMPAN DINGIN Ayu Sulvi Istanty; M Ade Salim; Nurul Isnaini; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

The purpose of this research wasinvestigated the substitution effect of Bovine Serum Albumin (BSA) with albumen on CEP-2 diluent to semen quality of Boer goat that was stored at 3-50C. Research was conducted at Reproduction Laboratory and Field Laboratory SumberSekarDau Animal Husbandry Faculty of Brawijaya University from December 2016until January 2017. The materials used for this research were fresh semen from 3,5-4,5 years old Boer goat which was collected with artificial vagina. Semen diluent was divided into two groups, those were P0 (90% CEP-2 + 10% Egg Yolk) and P1 (90% CEP-2 (without BSA) + 0,4% albumen + 10% Egg Yolk). Data of the research were analyzed using paired design t test. The result showed that after eight days of chilled preservation, the sperm motility of P0 was not significantly different with P1 (P>0,05). The average percentage of motility during eight days preservation P0 was higher than P1. The viability between P0 and P1 showed highly significantly difference (P<0,01). The average percentage of viability during eight days preservation P0 was higher than P1. The abnormality between P0 and P1 showed highly significant difference (P<0,01). The average percentage of abnormality between eight dayspreservation P1 was higher than P0. Total motile sperms count after seven days chilled preservation was not significantly different with hope value 40 million motile sperm/ml. The conclusion of this research was the substitution of BSA with albumen could maintain Boer goat semen quality. Keywords : CEP-2, Bovine SerumAlbumin, Semen, Cryoprotectant

PENGARUH LAMA SIMPAN SEMEN DENGAN PENGENCER TRIS AMINOMETHAN KUNING TELUR PADA SUHU RUANG TERHADAP KUALITAS SPERMATOZOA KAMBING BOER M Fajar Agustian; M Nur Ihsan; Nurul Isnaini
TERNAK TROPIKA Journal of Tropical Animal Production Vol 15, No 2 (2014): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

This study was conducted from May to June 2014 at Sumber Sekar Labolatory of Animal Husbandry Faculty, University of Brawijaya, Malang. The purpose of the study was to find out quality of semen Boer goat in room temperature with different storage duration. The result showed that storage treatment of semen with yolk tris aminhomethan diluents had highly signicant defferen as (P<0.01) on individual motility, viability and abnormality of semen. The conclusion of this result was optimal storage time was 240 minutes in room temperature with 53.50±2.42% of individual motility, 61.29±3.15% of viability and 15.15±2.42% of abnormality. Keyword: semen, Boar goat, tris aminomethane yolk, storage

PENGARUH PENAMBAHAN GLUTATHIONE PADA PENGENCER TRIS AMINOMETHANE KUNING TELUR DALAM MEMPERTAHANKAN KUALITAS SPERMATOZOA SAPI LIMOUSIN SELAMA PENYIMPANAN SUHU RUANG Rahman Maulana; Nurul Isnaini; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 17, No 1 (2016): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Penelitian ini dilaksanakan di Balai Besar Inseminasi Buatan (BBIB) Singosari pada bulan Maret sampai April 2016. Tujuan penelitian untuk mengetahui pengaruh pemberian glutathione pada pegencer Tris Aminomethane kuning telur dalam mempertahankan kualitas semen sapi Limousin selama penyimpanan suhu ruang. Materi yang digunakan adalah semen segar dengan motilitas individu 45-55%. Metode penelitian yang digunakan adalah percobaan. Metode percobaan dengan menggunakan Rancangan Acak Kelompok dan dianalisis dengan menggunakan analisis ragam. Perlakuan pada penelitian ini P0 ( 0 mM glutathione), P1 (0,5 mM glutathione), P2 (1 mM glutathione) dan P3 (1,25 mM glutathione) dengan 10 ulangan. Hasil penelitian menyatakan bahwa penambahan glutathione pada pengencer Tris Aminomethane kuning telur berbeda sangat nyata (P<0,01) persentase motilitas individu selama penyimpanan 3 dan 24 jam, sedangkan viabilitas dan abnormalitas spermatozoa pada semua perlakuan tidak berbeda nyata (P>0,05). Penyimpanan semen 3 jam dan 24 jam disuhu ruang sebaiknya menggunakan penambahan 0,75 mM glutathione dalam Tris Aminomethane kuning telur agar mempertahankan motilitas spermatozoa.Kata kunci : abnormalitas, Motilitas, Pengencer,semen, viabilitas

KUALITAS SEMEN SEGAR DAN RECOVERY RATE (RR) SAPI LIMOUSIN PADA MUSIM YANG BERBEDA Siti Sunami; Nurul Isnaini; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

This research aimed to determine the quality of fresh semen and recovery rate (RR) Limousin bulls at different seasons in Indonesia. The research materials were used semen sample and recording of 10 Limousin bulls on November 2015 until September 2016. The analyze was used by descriptive, while seasonal factors were determined by rainfall, they were divided into 2 stage (summer and rainy). The result was showed that the average of individual motility (60±0.07 %) on summer and (57.7±0.09 %) on rainy, Consentration of spermatozoa (1043±208 million/ml) on summer and (1013±175 million/ml) on rainy, post thawing motility (44±0.02 %) on summer and (41±0.05 %) on rainy, RR value (65±0.05 %) on summer and (55±0.09 %) on rainy, the production of frozen semen (1979±644.2 straw) on summer and (1623±379 straw) on rainy. It was concluded that the best quality of fresh until frozen semen was generally on summer with low rainfall. The seasonal effect for quality of semen Limousin bulls were individual motility, concentration, PTM, and RR value will be impacted for frozen semen produce. It was advisable to rejected the Limousin bulls with poor semen quality.Keywords: Seasonality, Summer, Rainy, Quality of Semen

PENGARUH LEVEL FILTRAT KECAMBAH KACANG HIJAU DALAM PENGENCER SUSU SKIM TERHADAP KUALITAS SEMEN CAIR PEJANTAN SAPI MADURA PADA PENYIMPANAN SUHU RUANG Yulia Indri Anastasia; Nurul Isnaini; Sri Wahjuningsih
TERNAK TROPIKA Journal of Tropical Animal Production Vol 16, No 2 (2015): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Tujuan dari penelitian ini yaitu untuk mengevaluasi pengaruh penambahan filtrat kecambah kacang hijau dalam pengencer susu skim terhadap kualitas spermetozoa pejantan sapi Madura pada penyimpanan suhu ruang. Materi dalam penelitian ini adalah semen segar sapi Madura berusia 6-11 tahun dengan persentase motilitas 50-65% diambil dari Balai Besar Inseminasi Buatan Singosari, Malang. Metode yang digunakann dalam penelitian ini adalah percobaan di laboratorium dengan rancangan acak kelompok yang terdiri dari empat level penambahan filtrat kecambah kacang hijau yaitu 0%, 2%, 4%, dan 6% dari jumlah 1ml pengencer susu skim dengan masing-masing 7 pengulangan dan disimpan pada suhu kamar selama 6 jam, setiap 2 jam dilakuakan evaluasi kualitas semen cair yaitu motilitas, viabilitas dan abnormalitas spermatozoa.Hasil penelitian menunjukkan bahwa pengaruh level penambahan filtrat kecambah kacang hijau dalam pengencer susu skim pada penyimpanan suhu ruang tidak memberikan perbedaan yang signifikan (P> 0,05) padamotilitas, viabilitas, dan abnormalitas spermatozoa. Kata kunci: semen cair, susu skim, filtrat kecambah kacang hijau, penyimpanan suhu ruang

PENGARUH PERBEDAAN KEMATANGAN AIR KELAPA HIJAU SEBAGAI BAHAN PENGENCER YANG DITAMBAH 10% KUNING TELUR TERHADAP KUALITAS SEMEN CAIR KAMBING BOER Rara Putri Audia; Muhammad Ade Salim; Nurul Isnaini; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

This study is conducted on December 2016until January 2017 in Sumber Sekar Field Laboratory, Faculty of Animal Husbandry, Brawijaya University. The purpose of this study is to determine the effect of different maturity levels of green coconut water dilution with 10% of egg yolk to the sperm quality of Boer goat during storage. Materials used in the study were male Boer kept in Sumber Sekar Field Laboratory. The semen was collected routinely once in 2 days using artificial vagina with fresh semen quality requirement individual motility ≥70%. The method used in this study is Randomnized Block Design which consists of three treatments, there are P0 as control (CEP-2 + 10% Egg Yolk); P1 (Young Green Coconut Water + 10% Egg Yolk) and P2 (Mature Green Coconut Water + 10% Egg Yolk). If the difference between the treatments showed real influence, then the Duncan’s Multiple Range Test will be done. The grouping is sorted by the semen observation time. The observed variables macroscopically include volume, smell, consistency, color and acidity, and microscopically which include individual motility, mass motility, viability and abnormality.The study result for the mean and standard deviation (SD) of motility in storage until the 4th day. The motility of P0 (36.67 ± 32.96)%, then P1 (20.0 ± 13.78)%, and the last P3 (18.33 ± 19.15). Dilution with the best viabillity average in the 4th day in P0 (58.02 ± 19.93)%, in P1 (26.10 ± 18.47)% and the last is P2 (25,87 ± 20,60)%.the average value of abnormality on the 4th day shows that the lowest average abnormality found in P0 (1.49 ± 0.73)%, then P1 (1.89 ± 1.68)% and the highest average value of abnormality is in P2 (2,87 ± 1.41).Keywords : liquid semen, boer goat, green coconut water, CEP-2, cold storage

PENGARUH AIR KELAPA MERAH YANG MUDA DAN TUA SEBAGAI PENGENCER TERHADAP KUALITAS SEMEN KAMBING BOER SELAMA PENYIMPANAN DINGIN Mugiyati Mugiyati; Nurul Isnaini; Muhammad Ade Salim; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

The aim of this research was to know the effect of maturity of red coconut water as a diluent to the sperms quality of Boer goats during chilled preservation. The research was carried out at Laboratory Sumber Sekar Faculty of Animal Husbandry University of Brawijaya Malang, on 11st December 2016 until 31st January 2017. The method used in this research was laboratory experimental using Randomized Block Design with three treatments, P0 (Tris Aminomethane + 20% Egg Yolk), P1 (Young Red Coconut Water + 20% Egg Yolk) and P2 (Mature Red Coconut Water + 20% Egg Yolk) which each treatment using 10 repetitions. The observed variables include the percentage of motility, percentage of viability and percentage of abnormal spermatozoa during chilled preservation The results showed that on the 3ndday the percentage of motility of spermatozoa during chilled preservation gave a significant effect (P<0.05), while on viability gave a very significant effect (P<0.01), and on abnormality did not give a significant effect (P> 0.05). The highest of total motile spermatozoa was P0 (Tris Aminomethan + 20% Egg Yolk). P2 better than P1 on motility, but not on viability and abnormality. The conclusions was the maturity of red coconut water can improve motility, but can not improve viability and abnormality. The suggested was use Mature Red coconut water + 20% Egg Yolk as a diluent and do further research on coconut water to be able to maintain spermatozoa longer.Keyword : Sperms, egg yolk, red coconut water, tris aminomethane

PENGARUH WAKTU SIMPAN SEMEN SEGAR DENGAN PENGENCER ANDROMED PADA SUHU RUANG TERHADAP KUALITAS SEMEN KAMBING BOER Try Puji Lestari; M Nur Ihsan; Nurul Isnaini
TERNAK TROPIKA Journal of Tropical Animal Production Vol 15, No 1 (2014): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

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This research was conducted from 20th Mei to 10th June 2014 at Sumber Sekar Field Laboratory Animal Husbandry Faculty Brawijaya University Malang. The aims of this experiment was to test the effect of semen storage duration diluted with Andromed solution at room temperature on the quality of Boer goat spermatozoa. The material was 1 boer goat semen aged 3 years old. Methods used experimental Randomized Design with 6 treatment and 10 replications by used Completely Randomized Design. The interval storage duration were 0, 60, 120, 180, 240 and 300 minutes. Data was analysed by analysis of variance. The result showed that interval storage duration of Boer goat semen diluted with Andromed solution were highly significant different (P<0,01) on sperm individual motility, sperm viability and sperm abnormality percentage. Optimal time for AI was 300 minutes at room temperature with 40±4,08% of individual motility and 57,94±1,71% of viability percentage. Optimal time for AI was 300 minutes in room temperature with 13,20±0,20% of sperm abnormality. The conclusion of this result was Boer goat semen diluted with Andromed at room temperature should be used not more than 300 minutes. Keyword: Boer goat, sperm motility, viability and sperm abnormality

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